LOCUS       NM_024130               4423 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus dynactin subunit 1 (Dctn1), mRNA.
ACCESSION   NM_024130
VERSION     NM_024130.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Guo,C., Williams,J.C. and Polenova,T.
  TITLE     Conformational Flexibility of p150Glued(1-191) Subunit of Dynactin
            Assembled with Microtubules
  JOURNAL   Biophys J 117 (5), 938-949 (2019)
   PUBMED   31445682
  REMARK    GeneRIF: Conformational Flexibility of p150(Glued)(1-191) Subunit
            of Dynactin Assembled with Microtubules.
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Villarin,J.M., McCurdy,E.P., Martinez,J.C. and Hengst,U.
  TITLE     Local synthesis of dynein cofactors matches retrograde transport to
            acutely changing demands
  JOURNAL   Nat Commun 7, 13865 (2016)
   PUBMED   28000671
  REMARK    GeneRIF: Data show that axonal synthesis of Lis1 and p150Glued is
            required for the adjustment of retrograde transport to acutely
            changing neurotrophin signalling in the periphery of neurons.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4423)
  AUTHORS   Nirschl,J.J., Magiera,M.M., Lazarus,J.E., Janke,C. and
            Holzbaur,E.L.
  TITLE     alpha-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate
            the Initiation of Dynein-Driven Transport in Neurons
  JOURNAL   Cell Rep 14 (11), 2637-2652 (2016)
   PUBMED   26972003
REFERENCE   4  (bases 1 to 4423)
  AUTHORS   Seldin,L., Muroyama,A. and Lechler,T.
  TITLE     NuMA-microtubule interactions are critical for spindle orientation
            and the morphogenesis of diverse epidermal structures
  JOURNAL   Elife 5, e12504 (2016)
   PUBMED   26765568
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4423)
  AUTHORS   Chen,T.Y., Syu,J.S., Han,T.Y., Cheng,H.L., Lu,F.I. and Wang,C.Y.
  TITLE     Cell Cycle-Dependent Localization of Dynactin Subunit p150 glued at
            Centrosome
  JOURNAL   J Cell Biochem 116 (9), 2049-2060 (2015)
   PUBMED   25774020
REFERENCE   6  (bases 1 to 4423)
  AUTHORS   Cassimeris,L. and Morabito,J.
  TITLE     TOGp, the human homolog of XMAP215/Dis1, is required for centrosome
            integrity, spindle pole organization, and bipolar spindle assembly
  JOURNAL   Mol Biol Cell 15 (4), 1580-1590 (2004)
   PUBMED   14718566
REFERENCE   7  (bases 1 to 4423)
  AUTHORS   Schroer,T.A.
  TITLE     Dynactin
  JOURNAL   Annu Rev Cell Dev Biol 20, 759-779 (2004)
   PUBMED   15473859
  REMARK    Review article
REFERENCE   8  (bases 1 to 4423)
  AUTHORS   Collin,G.B., Nishina,P.M., Marshall,J.D. and Naggert,J.K.
  TITLE     Human DCTN1: genomic structure and evaluation as a candidate for
            Alstrom syndrome
  JOURNAL   Genomics 53 (3), 359-364 (1998)
   PUBMED   9799602
REFERENCE   9  (bases 1 to 4423)
  AUTHORS   Waterman-Storer,C.M., Karki,S. and Holzbaur,E.L.
  TITLE     The p150Glued component of the dynactin complex binds to both
            microtubules and the actin-related protein centractin (Arp-1)
  JOURNAL   Proc Natl Acad Sci U S A 92 (5), 1634-1638 (1995)
   PUBMED   7878030
REFERENCE   10 (bases 1 to 4423)
  AUTHORS   Holzbaur,E.L., Hammarback,J.A., Paschal,B.M., Kravit,N.G.,
            Pfister,K.K. and Vallee,R.B.
  TITLE     Homology of a 150K cytoplasmic dynein-associated polypeptide with
            the Drosophila gene Glued
  JOURNAL   Nature 351 (6327), 579-583 (1991)
   PUBMED   1828535
  REMARK    Erratum:[Nature. 1992 Dec 17;360(6405):695. doi: 10.1038/360695a0..
            PMID: 1361213]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Nov 30, 2020 this sequence version replaced NM_024130.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X62160.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-295               JAXUCZ010000004.1  117241723-117242017
            296-541             JAXUCZ010000004.1  117244380-117244625
            542-620             JAXUCZ010000004.1  117244906-117244984
            621-655             JAXUCZ010000004.1  117245147-117245181
            656-676             JAXUCZ010000004.1  117245750-117245770
            677-694             JAXUCZ010000004.1  117248336-117248353
            695-715             JAXUCZ010000004.1  117249600-117249620
            716-907             JAXUCZ010000004.1  117250887-117251078
            908-1105            JAXUCZ010000004.1  117251375-117251572
            1106-1310           JAXUCZ010000004.1  117251715-117251919
            1311-1389           JAXUCZ010000004.1  117251993-117252071
            1390-1549           JAXUCZ010000004.1  117252196-117252355
            1550-1654           JAXUCZ010000004.1  117252458-117252562
            1655-1846           JAXUCZ010000004.1  117252676-117252867
            1847-1963           JAXUCZ010000004.1  117252948-117253064
            1964-2116           JAXUCZ010000004.1  117253276-117253428
            2117-2277           JAXUCZ010000004.1  117254182-117254342
            2278-2446           JAXUCZ010000004.1  117254432-117254600
            2447-2515           JAXUCZ010000004.1  117255532-117255600
            2516-2578           JAXUCZ010000004.1  117255773-117255835
            2579-2728           JAXUCZ010000004.1  117255932-117256081
            2729-2890           JAXUCZ010000004.1  117256212-117256373
            2891-3022           JAXUCZ010000004.1  117256448-117256579
            3023-3148           JAXUCZ010000004.1  117256760-117256885
            3149-3291           JAXUCZ010000004.1  117257287-117257429
            3292-3458           JAXUCZ010000004.1  117257654-117257820
            3459-3473           JAXUCZ010000004.1  117259037-117259051
            3474-3607           JAXUCZ010000004.1  117259196-117259329
            3608-3800           JAXUCZ010000004.1  117259437-117259629
            3801-3880           JAXUCZ010000004.1  117260119-117260198
            3881-3970           JAXUCZ010000004.1  117260665-117260754
            3971-4423           JAXUCZ010000004.1  117261076-117261528
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..4423
                     /gene="Dctn1"
                     /note="dynactin subunit 1"
                     /db_xref="GeneID:29167"
                     /db_xref="RGD:62038"
     exon            1..295
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     CDS             263..4108
                     /gene="Dctn1"
                     /note="DP-150; DAP-150; p150-glued; 150 kDa
                     dynein-associated polypeptide; dynactin 1"
                     /codon_start=1
                     /product="dynactin subunit 1"
                     /protein_id="NP_077044.2"
                     /db_xref="GeneID:29167"
                     /db_xref="RGD:62038"
                     /translation="MAQSKRHMYNRTPSGSRMSTEASARPLRVGSRVEVIGKGHRGTV
                     AYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGAD
                     TTSPETPDSSASKILKREGADAAAKTSKLRGLKPKKAPTARKTTTRRPKPTRPASTGV
                     AGPSSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPALTSPGAAPPLPSPSKEEE
                     GLRAQVRDLEEKLETLRLKRSEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRL
                     KEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDE
                     LTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQ
                     KLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNL
                     EEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQE
                     TVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHA
                     KAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRK
                     QAQEKFDLSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVY
                     KKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPE
                     ESTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFC
                     KKIRRRMPGTDAPGIPAALAFGSQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLA
                     ENEGLPVAALEELAFKASEQIYGSPSSSPYECLRQSCSILISTMNKLATAMQEGEYDA
                     ERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVR
                     LSLLEKKLDSAAKDADERIEKVQTRLEETQTLLRKKEKEFEETMDALQADIDQLEAEK
                     TELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEQQRGGTPGQAPGALPGPGPVK
                     DSPLLLQQISAMRLHISQLQHENSILRGAQMKASLAALPPLHVAKFSLPPHEGPGGNL
                     LSGALYRKTSQLLEKLNQLSTHTHVVDITRSSPAAKSPSAQLMEQVAQLKSLSDTIEK
                     LKDEVLKETVTQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGLGQR
                     HRLVLTQEQLHQLHGRLIS"
     exon            296..541
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            542..620
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..655
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            656..676
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            677..694
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            695..715
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            716..907
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1105
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1310
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1389
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1549
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1654
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1846
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1963
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2116
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2277
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2446
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2515
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..2578
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2579..2728
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2729..2890
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2891..3022
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3148
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3291
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3292..3458
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..3473
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3607
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3608..3800
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3801..3880
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3881..3970
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3971..4423
                     /gene="Dctn1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcagagtga gactcatcag ctggcgagcg gggctcagtg tctctgggtt caggaatcct
       61 tgggcagtag aagggactta ctcctcagcc ccatcactgc ccctacccct cccgagggag
      121 gcccatggat ggagggaggt ccgttgctga cggcttctga gccggtccat gcaggcccat
      181 caccctgacc cctcctgcct aggtttctgc ctttctctgt ctttggctag ctgggggagg
      241 gggtagagtc cgggtgagca acatggccca gagcaagagg cacatgtaca atcggacgcc
      301 cagtggcagc aggatgagta cggaggcaag cgcccggccc ctgcgggtcg gctcccgtgt
      361 ggaggtgatt gggaagggcc accgtggtac tgtggcctat gttggagcta cactctttgc
      421 cactggcaag tgggtgggcg tgatcctgga tgaagcaaaa ggcaagaacg atggtaccgt
      481 tcagggaagg aagtatttca catgcgatga aggccacggc atctttgtac gccaatccca
      541 gatccaagta tttgaagatg gagcagatac tacttcccca gagactcctg attcttctgc
      601 ttcaaagatc ctcaagagag agggagctga tgcagctgca aagaccagca aactgcgggg
      661 actgaagcct aagaaggcac cgacagcccg aaagaccaca actcgacggc ccaagcctac
      721 tcgcccagcc agtactgggg tggctgggcc cagtagctcc ttgggcccct ctggatcagc
      781 atcagccggt gaactgagca gcagtgagcc cagcacccca gctcagactc cgctggcagc
      841 acccatcatc cccacaccgg ccctcacctc tcctggagca gcacccccac ttccttctcc
      901 ctctaaggaa gaggaagggc tgagggccca ggtacgggac ctggaggaga agctggagac
      961 cttgcgtctg aaacgctcag aagacaaagc aaagctgaag gagctggaga agcacaagat
     1021 ccagctggag caggtgcagg aatggaagag caaaatgcag gagcagcagg cggacctgca
     1081 gcggcgcctc aaggaggcgc ggaaggaagc caaggaggca ctggaggcaa aggagcgcta
     1141 catggaggag atggcagaca cagccgacgc catcgagatg gccactctgg acaaggagat
     1201 ggccgaggag cgggccgagt ccctgcagca ggaggtggag gcactgaagg aacgagtaga
     1261 cgaactcacc acagacttgg agatcctcaa ggctgaaatt gaagagaaag gctctgatgg
     1321 ggctgcgtcc agctaccagc tcaaacagct ggaggagcag aatgcccgcc tgaaggacgc
     1381 cctggtgagg atgcgagatc tctcttcctc agagaagcag gagcatgtga agctccagaa
     1441 gctcatggaa aagaagaacc aggagctgga ggttgtgagg cagcagcgtg agcgtcttca
     1501 ggaggaactg agccaggcag agagcaccat cgatgagctc aaagagcagg tggacgctgc
     1561 tctgggagct gaggagatgg tggagatgct gactgaccgg aacctgaatc tggaggagaa
     1621 agtgcgagaa ttacgggaga ctgtggggga cttggaagcg atgaatgaga tgaatgacga
     1681 gctgcaggag aacgcacgcg agacagaact ggagctgcgt gagcagctgg acatggcagg
     1741 cgcccgagta agggaggccc agaagcgagt ggaagccgcc caggagacgg tcgccgacta
     1801 ccagcagacc atcaagaagt accgccagtt gactgcccac ctacaggatg tcaatcggga
     1861 gctgaccaac cagcaggaag cgtctgtgga gagacagcag cagccaccgc cagagacttt
     1921 tgatttcaaa atcaagtttg ctgagaccaa ggctcatgcc aaggcgatcg agatggagtt
     1981 gaggcagatg gaagttgccc aggccaaccg gcacatgtcc ctgctgacgg cctttatgcc
     2041 tgacagcttc cttcggccag ggggagacca tgactgtgtc ctggtgctgc tgctcatgcc
     2101 ccgactcatc tgcaaggcag agctcatccg gaagcaggcc caggagaagt ttgacctgag
     2161 tgagaactgt tcggagcggc ctgggctgcg aggggctgct ggggagcagc taagctttgc
     2221 cgccgggctg gtgtactcgc tgagtctgct gcaggccaca ctgcaccgct acgagcatgc
     2281 cctctctcag tgcagcgtgg acgtgtacaa gaaggtaggc agcctgtacc cggagatgag
     2341 tgcccacgag cgctccttag atttcctcat cgagctgctg cacaaggatc agctggacga
     2401 gactgtcaac gtggagcccc tcaccaaggc catcaagtac taccagcatc tgtacagcat
     2461 ccacctcgct gagcagcccg aggagtccac catgcagctg gctgaccaca tcaagttcac
     2521 ccagagtgcc ctggattgta tgagcgtgga ggtggggcgg ctgcgtgcct tcttgcaggg
     2581 tgggcaggag gcaacagata ttgctcttct tctgcgagac ctggaaacat cctgcagtga
     2641 catccgtcag ttctgcaaga agatccgacg gcgaatgcca gggacggatg ctcctgggat
     2701 cccagcagcg ctggccttcg gctcacaggt gtctgacaca ctcctggact gcaggaagca
     2761 cctgacgtgg gtggtagctg ttctgcagga ggtggcagct gcagccgccc agctcatcgc
     2821 ccccttggca gagaacgagg ggctgcccgt ggctgcactg gaggagctgg ctttcaaagc
     2881 aagcgagcag atctacggga gcccctccag cagcccctac gaatgtctgc gccagtcatg
     2941 ctccatcctc atcagcacga tgaacaagtt ggccacagcc atgcaggaag gcgagtatga
     3001 cgcagagcgg cccccgagca agcctcctcc agttgaactt cgggctgcag cactgcgtgc
     3061 agagatcaca gatgctgaag gtctgggttt gaagcttgag gatcgagaga cagtcatcaa
     3121 ggagttaaag aagtcactca agattaaggg agaggagctg agtgaggcca acgtgcggct
     3181 gagcctcctg gagaagaagc tggacagcgc tgccaaggat gctgatgagc gaatcgagaa
     3241 agttcagact cggctggagg agacgcagac cctgctacgg aagaaggaga aagagtttga
     3301 ggagacaatg gatgcactcc aggctgacat agaccagctg gaggcagaga agacagagct
     3361 caagcagcgc ctgaacagcc agtccaagcg cactattgag ggactccggg gcccccctcc
     3421 atcaggcatt gctaccctgg tctctggcat tgctggtgag gaacaacagc gagggggcac
     3481 tcctgggcag gctccaggcg ccttgccagg cccggggccg gtgaaggact caccactgct
     3541 gcttcagcag atctctgcca tgaggttaca catctctcag ctccagcatg agaacagcat
     3601 cctcagagga gcccagatga aggcgtcctt ggcagctctg ccccctctgc atgttgcaaa
     3661 gttttccctc ccaccccatg agggccctgg tggtaacctc ctgtctgggg cactgtatcg
     3721 caagaccagc cagctgttgg agaaactaaa ccaactgagc actcacaccc acgtagtaga
     3781 catcacccga agcagcccgg ctgccaagag cccatccgct cagcttatgg aacaagtggc
     3841 ccagctcaag tctctgagcg acaccatcga gaagctcaag gatgaggtcc tcaaggagac
     3901 agtaactcag cgtcctggag ctactgttcc cactgacttt gccaccttcc cttcatctgc
     3961 cttcctgagg gccaaggaag agcagcaaga cgacacagtc tacatgggca aagtgacctt
     4021 ctcgtgtgcg gcaggcctcg gacagcgaca ccgcctggtg ctgacccagg agcagctgca
     4081 ccagcttcac ggtcgtctca tctcctaagc actccttccc tgccttccct ttggtcccac
     4141 tgcccagtgc acggccacct cagccactcc cgggttgaac cacatgctaa cttcttgagt
     4201 ggttcacctt cgcctggtct ggtcagggtt ctgctacttc cgtaacctgg gtttgcctgt
     4261 ttacccctgg cctcttccat atttgctgtt catctgctcc ctctttcctg aggggcctca
     4321 gggcattgga gggggctgac acccctccac tacagattga gtgaggtctc cctgactgag
     4381 gacttcaccc cttgattaaa gcaacttctg cttcactgct ttc
//
