LOCUS       NM_153302               1236 bp    mRNA    linear   ROD 09-APR-2024
DEFINITION  Rattus norvegicus decapping enzyme, scavenger (Dcps), mRNA.
ACCESSION   NM_153302
VERSION     NM_153302.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Wypijewska,A., Bojarska,E., Lukaszewicz,M., Stepinski,J.,
            Jemielity,J., Davis,R.E. and Darzynkiewicz,E.
  TITLE     7-methylguanosine diphosphate (m(7)GDP) is not hydrolyzed but
            strongly bound by decapping scavenger (DcpS) enzymes and potently
            inhibits their activity
  JOURNAL   Biochemistry 51 (40), 8003-8013 (2012)
   PUBMED   22985415
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Singh,J., Salcius,M., Liu,S.W., Staker,B.L., Mishra,R.,
            Thurmond,J., Michaud,G., Mattoon,D.R., Printen,J., Christensen,J.,
            Bjornsson,J.M., Pollok,B.A., Kiledjian,M., Stewart,L., Jarecki,J.
            and Gurney,M.E.
  TITLE     DcpS as a therapeutic target for spinal muscular atrophy
  JOURNAL   ACS Chem Biol 3 (11), 711-722 (2008)
   PUBMED   18839960
REFERENCE   3  (bases 1 to 1236)
  AUTHORS   Liu,S.W., Rajagopal,V., Patel,S.S. and Kiledjian,M.
  TITLE     Mechanistic and kinetic analysis of the DcpS scavenger decapping
            enzyme
  JOURNAL   J Biol Chem 283 (24), 16427-16436 (2008)
   PUBMED   18441014
REFERENCE   4  (bases 1 to 1236)
  AUTHORS   Shen,V., Liu,H., Liu,S.W., Jiao,X. and Kiledjian,M.
  TITLE     DcpS scavenger decapping enzyme can modulate pre-mRNA splicing
  JOURNAL   RNA 14 (6), 1132-1142 (2008)
   PUBMED   18426921
REFERENCE   5  (bases 1 to 1236)
  AUTHORS   Kwasnicka-Crawford,D.A. and Vincent,S.R.
  TITLE     Role of a novel dual flavin reductase (NR1) and an associated
            histidine triad protein (DCS-1) in menadione-induced cytotoxicity
  JOURNAL   Biochem Biophys Res Commun 336 (2), 565-571 (2005)
   PUBMED   16140270
REFERENCE   6  (bases 1 to 1236)
  AUTHORS   Cohen,L.S., Mikhli,C., Friedman,C., Jankowska-Anyszka,M.,
            Stepinski,J., Darzynkiewicz,E. and Davis,R.E.
  TITLE     Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in
            Ascaris embryos and characterization of C. elegans scavenger DcpS
  JOURNAL   RNA 10 (10), 1609-1624 (2004)
   PUBMED   15383679
REFERENCE   7  (bases 1 to 1236)
  AUTHORS   Kwasnicka,D.A., Krakowiak,A., Thacker,C., Brenner,C. and
            Vincent,S.R.
  TITLE     Coordinate expression of NADPH-dependent flavin reductase, Fre-1,
            and Hint-related 7meGMP-directed hydrolase, DCS-1
  JOURNAL   J Biol Chem 278 (40), 39051-39058 (2003)
   PUBMED   12871939
REFERENCE   8  (bases 1 to 1236)
  AUTHORS   Liu,H., Rodgers,N.D., Jiao,X. and Kiledjian,M.
  TITLE     The scavenger mRNA decapping enzyme DcpS is a member of the HIT
            family of pyrophosphatases
  JOURNAL   EMBO J 21 (17), 4699-4708 (2002)
   PUBMED   12198172
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Dec 5, 2020 this sequence version replaced NM_153302.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089773.1, AF411249.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760475,
                                           SAMN12840106 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-226               JAXUCZ010000008.1  41781997-41782222   c
            227-401             JAXUCZ010000008.1  41777650-41777824   c
            402-547             JAXUCZ010000008.1  41741920-41742065   c
            548-661             JAXUCZ010000008.1  41734460-41734573   c
            662-772             JAXUCZ010000008.1  41731436-41731546   c
            773-1236            JAXUCZ010000008.1  41729507-41729970   c
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q21"
     gene            1..1236
                     /gene="Dcps"
                     /gene_synonym="DCS-1; Hint-5"
                     /note="decapping enzyme, scavenger"
                     /db_xref="GeneID:266605"
                     /db_xref="RGD:628887"
     exon            1..226
                     /gene="Dcps"
                     /gene_synonym="DCS-1; Hint-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..1039
                     /gene="Dcps"
                     /gene_synonym="DCS-1; Hint-5"
                     /EC_number="3.6.1.59"
                     /note="scavenger mRNA-decapping enzyme DcpS; hint-related
                     7meGMP-directed hydrolase; histidine triad protein member
                     5; mRNA decapping enzyme; decapping scavenger enzyme;
                     histidine triad nucleotide-binding protein 5"
                     /codon_start=1
                     /product="m7GpppX diphosphatase"
                     /protein_id="NP_695214.2"
                     /db_xref="GeneID:266605"
                     /db_xref="RGD:628887"
                     /translation="MADTAPQLKRKREPEPEEAETPSTEKEAGVGNGTSAPVRLPFSG
                     FRVQKVLRESARDKIIFLHGKVNEDSGDTHGEDAVVILEKTPFQVEHVAQLLTGNPEL
                     KLQFSNDIYSTYNLFPPRHLSDIKTTVVYPATEKHLQKYMRQDLRLIRETGDDYRSLT
                     LPYLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIA
                     ICHRRGIRSLRDLTPEHLPLLRNILREGQEAILKRYQVTGDRLRVYLHYLPSYYHLHV
                     HFTALGFEAPGSGVERAHLLAEVIENLECDPKHYQRRTLTFALRTDDPLLQLLQKAQQ
                     P"
     exon            227..401
                     /gene="Dcps"
                     /gene_synonym="DCS-1; Hint-5"
                     /inference="alignment:Splign:2.1.0"
     exon            402..547
                     /gene="Dcps"
                     /gene_synonym="DCS-1; Hint-5"
                     /inference="alignment:Splign:2.1.0"
     exon            548..661
                     /gene="Dcps"
                     /gene_synonym="DCS-1; Hint-5"
                     /inference="alignment:Splign:2.1.0"
     exon            662..772
                     /gene="Dcps"
                     /gene_synonym="DCS-1; Hint-5"
                     /inference="alignment:Splign:2.1.0"
     exon            773..1236
                     /gene="Dcps"
                     /gene_synonym="DCS-1; Hint-5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgaaggcgg gttgggttcg tcccaggcag catggcggat acagcgcctc aactcaagag
       61 aaagcgcgaa ccggagccag aggaggcaga aacccccagc acagagaagg aagcaggcgt
      121 tggcaatggt acctctgccc ctgtccgctt acccttctcc ggctttagag tacaaaaagt
      181 gctgagggag tctgcgcggg acaaaattat tttcctgcat gggaaggtga atgaggactc
      241 tggggatact catggagaag atgctgtggt aatcctggag aagacaccgt ttcaggtaga
      301 acatgtggcg cagctcctaa cggggaaccc agagctcaag ttgcaattct ccaatgatat
      361 ctacagcacc tataacctgt ttcctccaag gcatctgagt gatataaaaa caaccgtggt
      421 ataccctgcc acagagaaac acctgcaaaa gtacatgcgt caggacctcc gcctgatccg
      481 agagactgga gatgactaca ggagcctcac tttaccctac ctggagtccc agagcctcag
      541 catccagtgg gtatataaca ttcttgacaa gaaggccgaa gctgaccgga ttgtctttga
      601 gaacccagat ccttctgatg gctttgtcct catcccagac ctcaaatgga accagcagca
      661 gcttgatgac ctgtacttga tcgccatttg ccatcgccgg ggtatcagat cacttcgaga
      721 tctcactcca gagcacctgc cactactgag gaacattctc cgagaaggac aagaagccat
      781 cctgaagcgc taccaggtaa caggagaccg tctgcgtgtg tacctgcact acctgccctc
      841 ttactaccac ctgcatgtac atttcaccgc cctgggtttc gaggctcctg gctcaggtgt
      901 ggagagggcg cacctgctgg ctgaagtgat cgagaacctg gagtgtgacc ccaagcatta
      961 tcagcggcgc actcttactt tcgccctcag gaccgacgac cccctgcttc agctcctgca
     1021 gaaggcccag caaccctgag ttgttgagtg cctgaagaga ggggaactag tgggttgggc
     1081 ttggggtagt gtgggctggg tttttatctc taagggattt tcctaaaatg tattttgtag
     1141 tttcttggcc tttcagcaaa ggtcaaatgt aagctgggtt tttttgtact ggtttattcc
     1201 tcacatgaga atgaagaact ttcttattgg tgagga
//
