LOCUS       NM_053343               6903 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus doublecortin-like kinase 1 (Dclk1), transcript
            variant 1, mRNA.
ACCESSION   NM_053343
VERSION     NM_053343.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Vitali,F., Tortora,K., Di Paola,M., Bartolucci,G., Menicatti,M., De
            Filippo,C. and Caderni,G.
  TITLE     Intestinal microbiota profiles in a genetic model of colon
            tumorigenesis correlates with colon cancer biomarkers
  JOURNAL   Sci Rep 12 (1), 1432 (2022)
   PUBMED   35082322
  REMARK    GeneRIF: Intestinal microbiota profiles in a genetic model of colon
            tumorigenesis correlates with colon cancer biomarkers.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Nakane,T., Matsumoto,S., Iida,S., Ido,A., Fukunaga,K., Murao,K. and
            Sugiyama,Y.
  TITLE     Candidate plasticity gene 16 and jun dimerization protein 2 are
            involved in the suppression of insulin gene expression in rat
            pancreatic INS-1 beta-cells
  JOURNAL   Mol Cell Endocrinol 527, 111240 (2021)
   PUBMED   33676985
  REMARK    GeneRIF: Candidate plasticity gene 16 and jun dimerization protein
            2 are involved in the suppression of insulin gene expression in rat
            pancreatic INS-1 beta-cells.
REFERENCE   3  (bases 1 to 6903)
  AUTHORS   Nakane,T., Ido,A., Higuchi,T., Todaka,H., Morisawa,K., Nagamine,T.,
            Fukunaga,K., Sakamoto,S., Murao,K. and Sugiyama,Y.
  TITLE     Candidate plasticity gene 16 mediates suppression of insulin gene
            expression in rat insulinoma INS-1cells under glucotoxic conditions
  JOURNAL   Biochem Biophys Res Commun 512 (2), 189-195 (2019)
   PUBMED   30879761
  REMARK    GeneRIF: These results suggest that CPG16 plays a role in the
            suppression of insulin gene expression in pancreatic beta-cells
            under glucotoxic conditions.
REFERENCE   4  (bases 1 to 6903)
  AUTHORS   Quan,X., Huang,L., Yang,Y., Ma,T., Liu,Z., Ge,J., Huang,J. and
            Luo,Z.
  TITLE     Potential Mechanism of Neurite Outgrowth Enhanced by Electrical
            Stimulation: Involvement of MicroRNA-363-5p Targeting DCLK1
            Expression in Rat
  JOURNAL   Neurochem Res 42 (2), 513-525 (2017)
   PUBMED   27900578
  REMARK    GeneRIF: Electrical stimulated-neurite outgrowth is regulated by
            MicroRNA-363-5p through targeting of DCLK1.
REFERENCE   5  (bases 1 to 6903)
  AUTHORS   Lipka,J., Kapitein,L.C., Jaworski,J. and Hoogenraad,C.C.
  TITLE     Microtubule-binding protein doublecortin-like kinase 1 (DCLK1)
            guides kinesin-3-mediated cargo transport to dendrites
  JOURNAL   EMBO J 35 (3), 302-318 (2016)
   PUBMED   26758546
  REMARK    GeneRIF: The authors found that microtubule-binding protein
            doublecortin-like kinase 1 (DCLK1) labels a subset of dendritic
            microtubules and is required for KIF1-dependent dense-core vesicles
            (DCVs) trafficking into dendrites and dendrite development.
REFERENCE   6  (bases 1 to 6903)
  AUTHORS   Lin,P.T., Gleeson,J.G., Corbo,J.C., Flanagan,L. and Walsh,C.A.
  TITLE     DCAMKL1 encodes a protein kinase with homology to doublecortin that
            regulates microtubule polymerization
  JOURNAL   J Neurosci 20 (24), 9152-9161 (2000)
   PUBMED   11124993
REFERENCE   7  (bases 1 to 6903)
  AUTHORS   Mizuguchi,M., Qin,J., Yamada,M., Ikeda,K. and Takashima,S.
  TITLE     High expression of doublecortin and KIAA0369 protein in fetal brain
            suggests their specific role in neuronal migration
  JOURNAL   Am J Pathol 155 (5), 1713-1721 (1999)
   PUBMED   10550327
REFERENCE   8  (bases 1 to 6903)
  AUTHORS   Vreugdenhil,E., Datson,N., Engels,B., de Jong,J., van
            Koningsbruggen,S., Schaaf,M. and de Kloet,E.R.
  TITLE     Kainate-elicited seizures induce mRNA encoding a CaMK-related
            peptide: a putative modulator of kinase activity in rat hippocampus
  JOURNAL   J Neurobiol 39 (1), 41-50 (1999)
   PUBMED   10213452
REFERENCE   9  (bases 1 to 6903)
  AUTHORS   Berke,J.D., Paletzki,R.F., Aronson,G.J., Hyman,S.E. and Gerfen,C.R.
  TITLE     A complex program of striatal gene expression induced by
            dopaminergic stimulation
  JOURNAL   J Neurosci 18 (14), 5301-5310 (1998)
   PUBMED   9651213
REFERENCE   10 (bases 1 to 6903)
  AUTHORS   Hevroni,D., Rattner,A., Bundman,M., Lederfein,D., Gabarah,A.,
            Mangelus,M., Silverman,M.A., Kedar,H., Naor,C., Kornuc,M.,
            Hanoch,T., Seger,R., Theill,L.E., Nedivi,E., Richter-Levin,G. and
            Citri,Y.
  TITLE     Hippocampal plasticity involves extensive gene induction and
            multiple cellular mechanisms
  JOURNAL   J Mol Neurosci 10 (2), 75-98 (1998)
   PUBMED   9699150
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000002.1.
            
            On Sep 30, 2010 this sequence version replaced NM_053343.2.
            
            Summary: This gene encodes a member of the protein kinase
            superfamily and the doublecortin family. The typical protein
            encoded by this gene contains two N-terminal doublecortin domains,
            which bind microtubules and regulate microtubule polymerization, a
            C-terminal serine/threonine protein kinase domain, which shows
            substantial homology to Ca2+/calmodulin-dependent protein kinase,
            and a serine/proline-rich domain in between the doublecortin and
            the protein kinase domains, which mediates multiple protein-protein
            interactions. The microtubule-polymerizing activity of the protein
            is independent of its protein kinase activity. This gene is
            involved in several different cellular processes, including
            neuronal migration, retrograde transport, neuronal apoptosis and
            neurogenesis. Multiple transcript variants generated by two
            alternative promoter usage and alternative splicing have been
            found, but the full-length nature of the variant produced from the
            5' promoter has not been determined. Current reference sequence
            data represents two alternatively spliced transcript variants
            produced from the 3' promoter and their protein products lack the
            doublecortin domain.[provided by RefSeq, Sep 2010].
            
            Transcript Variant: Transcript Variant: This variant [1, also known
            as DCLK-short-A, DCK-beta2, KIAA0369-BL or cpg16 (candidate
            plasticity gene 16)] is produced from the 3' promoter. It encodes
            isoform 1, which contains a serine/proline-rich domain and a
            protein kinase domain.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U78857.1, SRR26643298.10973.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on computational evidence
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               JAXUCZ010000002.1  141782884-141783048
            166-260             JAXUCZ010000002.1  141783938-141784032
            261-345             JAXUCZ010000002.1  141797855-141797939
            346-454             JAXUCZ010000002.1  141800437-141800545
            455-512             JAXUCZ010000002.1  141807038-141807095
            513-632             JAXUCZ010000002.1  141807519-141807638
            633-779             JAXUCZ010000002.1  141809444-141809590
            780-913             JAXUCZ010000002.1  141820350-141820483
            914-991             JAXUCZ010000002.1  141822327-141822404
            992-1088            JAXUCZ010000002.1  141822759-141822855
            1089-1169           JAXUCZ010000002.1  141826323-141826403
            1170-1283           JAXUCZ010000002.1  141839057-141839170
            1284-6903           JAXUCZ010000002.1  141855469-141861088
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q26"
     gene            1..6903
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /note="doublecortin-like kinase 1"
                     /db_xref="GeneID:83825"
                     /db_xref="RGD:68437"
     exon            1..165
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..1448
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     double cortin and calcium/calmodulin-dependent protein
                     kinase-like 1; serine/threonine-protein kinase DCLK1;
                     doublecortin-like and CAM kinase-like 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase DCLK1 isoform 1"
                     /protein_id="NP_445795.1"
                     /db_xref="GeneID:83825"
                     /db_xref="RGD:68437"
                     /translation="MLELIEVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRISQHGG
                     SSTSLSSTKVCSSMDENDGPGEEESDEGFQIPATITERYKVGRTIGDGNFAVVKECIE
                     RSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMEL
                     VKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDG
                     SKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCG
                     FPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLVNVDQRFSAVQV
                     LEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPSSTAAGVSVIATTALDKERQVFRR
                     RRNQDVRGRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF"
     exon            166..260
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            261..345
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            346..454
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            455..512
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            513..632
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            633..779
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            780..913
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            914..991
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1088
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1169
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1283
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..6903
                     /gene="Dclk1"
                     /gene_synonym="Ania4; Cpg16; Dcamkl1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cctgcatctc attcagcccg ctccccgagc agccttagcc atccctaact aatccaactg
       61 cttcccagtc tgcgggtgct gggctcggtg ctgagaagag gctctggctc ttggctattg
      121 tcaggtcaaa cactaagact gtgtccatgt tagaactcat agaagttaat ggaacccctg
      181 gcagtcagct ctccactccg cgctccggca agtcaccaag tccatcgccc accagcccag
      241 gaagcctgcg gaagcagagg atctctcagc atggcggctc ctccacttca ctttcatcca
      301 caaaagtctg cagctcaatg gatgagaacg atggccctgg ggaagaagag tccgacgaag
      361 gtttccagat tcctgccaca ataacagaga gatacaaagt cgggagaaca ataggagatg
      421 gaaatttcgc tgttgtcaag gaatgtatag agaggtcgac tgctcgggag tatgccctga
      481 aaattatcaa gaaaagtaaa tgccgaggca aagaacacat gatccaaaac gaggtctcca
      541 tcttaaggag agtgaagcat ccgaacattg ttctcctgat tgaagagatg gatgtgccga
      601 ctgaactata tcttgtaatg gagttagtaa agggtggaga ccttttcgat gccatcactt
      661 ccactagcaa atacacagag cgggatgcca gtgggatgct gtacaacctg gccagtgcca
      721 ttaaatacct gcacagcctg aacatcgtcc atcgtgacat caagccagag aacctgctgg
      781 tgtatgagca ccaggatggc agtaagtcac tcaagttggg tgactttggc ctggccacaa
      841 ttgtcgatgg ccccctgtac acagtctgtg gcaccccaac ttatgtggct ccagaaatca
      901 ttgcagagac tggatatggc ctcaaggtgg acatctgggc agctggcgtg atcacttata
      961 tcctgttgtg tggtttccct ccattccgtg gaagtgggga tgaccaggag gtgctttttg
     1021 accagatctt gatgggccaa gtggactttc catctccgta ttgggacaat gtgtcagatt
     1081 ctgctaagga gctcatcaac atgatgctgt tggttaacgt ggaccagaga ttttcagccg
     1141 tgcaagtcct tgagcacccc tgggttaacg atgatggcct cccagaaaat gaacatcagc
     1201 tgtcagtagc tggcaagatc aagaagcatt tcaacacggg ccccaagccg agcagcacgg
     1261 cagctggagt ttctgtaata gcaaccaccg ctcttgataa ggagaggcag gttttccgac
     1321 gaagacgcaa ccaggatgtg aggggccggt acaaggcaca gccagctcca ccagaactca
     1381 actcggaatc agaagactac tcccccagct cctctgagac tgtccgctcc cccaattcgc
     1441 ccttttaata agaccccttc actcgaagtc ctagcttaac cctttaagac tctgagattc
     1501 cccctccccc caaacttctg taaaacaggt tcatctgatc tacctagcac tcagtgctga
     1561 acggcagaac agaaagtata tttcgggtac ctttgtagca agttcccttt actgaatgag
     1621 aaggcatgtg gtgtttgtga caacggtaat ttgctgctaa taggggtcct taaagggttg
     1681 aggctaattt gcgatttttt ttcttttaac atagaagtaa cgaatgtttt catcagtcag
     1741 actaggatct gcagtatgta acatagcaca tgttagccct ctgagtgcat agaatttgct
     1801 tgagaatcct tgctggggaa tttttcagac ccttgagtgt attcacacat atttcttgat
     1861 tttactgcag accaaggggc gtcattgaga cccaaaatgt ccacccaaga agagtattgg
     1921 ggaatgacac attgccaatt cttctcagtg ggcttagaca atgacgggtt ctacagcatc
     1981 agcagatgca cccctccttc ttagccatct tatgaagaag ggaagtccct ttccctacat
     2041 agtaagctgc agagccttct ctgctctgat tggagggagt taaggtttgt ggctgcctat
     2101 cccaagtgat cgacagtgtc tctccctgtc tccattcact gctgtgctga aggcgcccct
     2161 gaaatgctgc ttccccatta gctgctgcta gtgccaggca gaccctcggg gaaccaccta
     2221 gccttggctt gtgctcggtg agttgggatc tgtctgctca gggtcagaag agctctagaa
     2281 acaactggtg agaagagcaa taaattatca ggtgctctac ggggccggga atcaaccggc
     2341 aaggaaaagc aaggctctgt gttgttgttg ttgtcatttt cttttctcag ctgcttttac
     2401 acaggaccat agccaccagt gcgaaaccaa cacagaatgg aaaagacccg acccggggca
     2461 tgtgagagca agaggcaagt gctttgcaaa gaggctgaaa ggaaaataca gagaaaacac
     2521 aatccaaaag gttctaaaat gggattccta gaaccaaaca gattccatga gtaactctag
     2581 gtaccaggaa gtcagttcca gcaagtctcc cagaagatac ctcagaggct gaagccacca
     2641 ctcccactca tgattttctt tactttattt agcatggtct ctagtcctcc aggagggatg
     2701 tgttgctttt ctggtttggg gccaagcctg gctgggattg ggttttcact cctgttaggc
     2761 aatctgtctc ggaggcctgg gtttccttgg gagcccattg tttcagaatg taggcttgtg
     2821 cttccttttc cttacttccc tgctccactg ggatgcagga gtgtgtggga gggtgtggga
     2881 ggcaaggccc aacccaagcg ttgccctaat gttttctcag ctttgactca agagagaaca
     2941 attttaaact ccagggtggc tacaagttcc ttatttgctc attactgctt tgaatgtatt
     3001 tcaatgtcct gaaagtcatg aacaaccttc ccatagtcag gtcgagatat ttccgaactc
     3061 cacactgatc tgcagctgct gtgtttatac atagtcatta aactatgttc ctcacacatg
     3121 tagattttta aataacagaa tcccatattt cctgctcatt taaatataac atttatttag
     3181 ttttttttcc ttttttggga gtccagaaat gatacacata aattcacaaa atgctataaa
     3241 tccatatatt atttgaattg ggtgctggga ctagagacag ggcacagacc accaggaagg
     3301 gtgtcgattt gtagatagag tccatatagg taaaggacaa gaactgctct aaaattgcta
     3361 ctttctctgg aacggaaaag caaagcgcta ttctaagatc agcgagcaat ggcatggctg
     3421 agccttggct ccacgtgtgt gactataaaa caccctacgg ccaatagcag attgccactt
     3481 tccttgttct gaattactgc tttccccggt cattaacgta gccacagcgt ccaggggtta
     3541 caggaccaaa gtgcaaggct ctggaatttt tccatcaaca ttctctcatc acgaagaagg
     3601 gcagctcaga agaattcaga gcccattcag aaggacgatc gcagcacatc cctatagaat
     3661 ttcagtgatc aacgctgaat aagtagcgga atgtaacctg tcaagtagaa atattgagca
     3721 atcagacgga atgcagtaat tcaagagtct tgccagcgtg gagccaccaa gatcttagac
     3781 gtcagagttg taactcagag ggcgaaggga cagcacacac tgaagactgg gtgcatccag
     3841 attgctttgc tttcagtcaa cacggatatc ctccccctca acattcccag aatagctcca
     3901 gtttgaaata attaagtggt ggtgttcttt gaatttctat aaccaatcaa tcttattttt
     3961 tattttgtgt atcatagaac aactatgtgc cactatggct gtgtgtcgcc gtaattctcc
     4021 actgaactat catgactgtt tgcctatctt tgagtattga atataactct tatggtggta
     4081 gtctggtgat gggacttgcc cttccttacc aacaagacca gtcttgtggt attggaaagg
     4141 ttctttcggt ttgagagtag agtagttgaa cactccttca ttacttgctt cctgtaaatc
     4201 aattctgtag atgagaacca ttatactaat gagtacattt ccccctcaac attgtagaag
     4261 gaaccacttt atttgtcatg atcaataaat atgtgaattg ttccaaactg ttagaagaaa
     4321 acgttaaggt cttcagtccc tgggaatgct ctgactgtag ccccccgcta acccacacgt
     4381 ggtggtggtg tgtattaata tgagtccagg ggaaatatga ataaacgacg gaaaacactt
     4441 gatgctctgc gttcctgagg cttccctgca gtctgcttag cccttttatg agtggtggtt
     4501 aacaggcctg tcacatagtg ttgagagagc gcagtatatc tgtgaaagaa ggccaaaatt
     4561 gcaatctttt ccccttccag gcatatttaa gttgggaaaa aaaaacagga aagaaaaatt
     4621 gtctcagagg gccacggagc taccgaatcg ccaggactca agcacgttaa atcatggttg
     4681 tttgccgacc acaggcatcg cctagacaac tctatcccct gtgctgaagt gcagttgtgc
     4741 tgaggggaaa ctgtctccac attattgtgc tgcctctagc ccttctctgg gtgtcagcat
     4801 ttccatgata aactcctatt ctggaaggtt tttaatttga ccataaaaat gtgcctcagg
     4861 ctgaggtttg ccattcgagg cctgttcaga agagggaagg ggcacatctc acccttctca
     4921 acttctcctc cgctcccgag aaagggtcca ccccgagatg tctgattgca tagccttcat
     4981 tgtgattcca gagtcttgga atgcgccaag atccaagatg gtaattttaa caaaactatc
     5041 cattcggata tgttctctcc aaatggctca actaacatat attataagat attttttatt
     5101 atttttaact ttttaattac tatgattctc cctctctctc cctctctgtc tctccttgtc
     5161 cctctctctg tctctctctt tgtccctctc tctgtctctc tctttgtccc tctctctgtc
     5221 tctctctttg tccctctctt tgtccctctc tgtgtctttc tttgcccacc ccacgcctct
     5281 tctccctctg tccaaatatt tttacgaact tgaacttgat cagcccaaag gtccactttt
     5341 cccatgagat gcctgctact tccattggga atttgcaaca ataactctct gccccagggg
     5401 agcactggtt tgcatgctgt gtggaggatc agatacatgc ttctttagaa atctgggttt
     5461 ccggagttta atcatcagat ttcatgaagg cactctagtc tattgggaga atagcggtcc
     5521 aaggagcccc cacaattaac acttttttca actcagattc gaagccaacg taaagtaata
     5581 cacttcctac tctcccgctt aaaggcagat aaatcagaga aacttgagga catttgaaac
     5641 atttgatgac cgcaaaagct ctttgcactt gggctctctg gtgcttacac ggggagcagt
     5701 taggaactgg gaccctggga tgtcagatgg aatctttagt ttgtaattac atacagatgg
     5761 gggaggtgct ttaagccgct cttttgtttt ttaatcatct caaatatgca gccagccagc
     5821 cagtagcact aagggtcata aactgatggg aatcaggaaa tccaaggtag aggtttagaa
     5881 tgcctcactc agctcgggca tttttgttcg tttctgtggc tggctctaac atggagacct
     5941 ctgtggtcat ttttcggtaa agtgagcatc taactggcag gtgttgtgat cttgcagttt
     6001 gcccgtggag acaaacaagg ggtgtggatt tattgctttc cgtcaagaaa aggcagtctc
     6061 tcagccgcac ctggaaacct cctccaccct tttttattta tttatttttt ggtcaactaa
     6121 actgagaccc ttaatgttac tttaatgtaa aattgtatat ttttgactgt gtcatacttt
     6181 actaatttta agtaattagt gcttaagcct tcagaactat tggtattaag agtaggatgt
     6241 taaagtggta ctaaacaact taatgctaat tcattccatc gtgctcggct gtagtcaggg
     6301 acagactggg aatagttctc acttgcccct gacattaaat ccttggtgtg ttcgaagaat
     6361 aaataaagag aaactgaagc tggttcaagg ttagcgtcat atccttgaca actttttttt
     6421 ttaatttttt tattaggaaa ttaatattag cctttttcat tctggctgaa agaaaattat
     6481 taaaagatta gttgagtgtg aaattgaata gtcctttacc cctgcatact aaagaggtgg
     6541 gctggggact ggacacattt aacaagttcc cctcaaggtt ttgatttgga gaaaacaatt
     6601 caacgtttcc tttggagagt cgcccttgtc acttgctgca tggattggat gacgtaggtt
     6661 agtgtctgat tttggaatcc caatgagata actttcaaac aatttatccc cataattaaa
     6721 ggtggaatgg tatccaattt ccccccaatc cttcagttta agctcataaa ttgtaggtga
     6781 atgtggaatg aaatcatctg tgatatatta tgttcattta ccaaatgagc tctttttgat
     6841 gttgcctgtt tttatgtaaa acatgttctt aaactaataa agtaatatct cttggtgtac
     6901 aaa
//
