LOCUS       NM_024158               4538 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus deoxycytidine kinase (Dck), mRNA.
ACCESSION   NM_024158
VERSION     NM_024158.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Wang,L. and Eriksson,S.
  TITLE     Tissue specific distribution of pyrimidine deoxynucleoside salvage
            enzymes shed light on the mechanism of mitochondrial DNA depletion
  JOURNAL   Nucleosides Nucleotides Nucleic Acids 29 (4-6), 400-403 (2010)
   PUBMED   20544526
  REMARK    GeneRIF: A cytosolic form of TK2 was identified in all tested
            tissues in addition to mitochondrial TK2. TK1 was detected in liver
            and spleen cytosolic extracts while dCK was found in liver, spleen
            and lung cytosolic extracts.
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Sabini,E., Ort,S., Monnerjahn,C., Konrad,M. and Lavie,A.
  TITLE     Structure of human dCK suggests strategies to improve anticancer
            and antiviral therapy
  JOURNAL   Nat Struct Biol 10 (7), 513-519 (2003)
   PUBMED   12808445
REFERENCE   3  (bases 1 to 4538)
  AUTHORS   Saada,A., Shaag,A., Mandel,H., Nevo,Y., Eriksson,S. and Elpeleg,O.
  TITLE     Mutant mitochondrial thymidine kinase in mitochondrial DNA
            depletion myopathy
  JOURNAL   Nat Genet 29 (3), 342-344 (2001)
   PUBMED   11687801
REFERENCE   4  (bases 1 to 4538)
  AUTHORS   Stegmann,A.P., Honders,W.H., Willemze,R., Ruiz van Haperen,V.W. and
            Landegent,J.E.
  TITLE     Transfection of wild-type deoxycytidine kinase (dck) cDNA into an
            AraC- and DAC-resistant rat leukemic cell line of clonal origin
            fully restores drug sensitivity
  JOURNAL   Blood 85 (5), 1188-1194 (1995)
   PUBMED   7532033
REFERENCE   5  (bases 1 to 4538)
  AUTHORS   Stegmann,A.P., Honders,M.W., Willemze,R. and Landegent,J.E.
  TITLE     Cloning of the Dck gene encoding rat deoxycytidine kinase
  JOURNAL   Gene 150 (2), 351-354 (1994)
   PUBMED   7821805
REFERENCE   6  (bases 1 to 4538)
  AUTHORS   Stegmann,A.P., Honders,M.W., Kester,M.G., Landegent,J.E. and
            Willemze,R.
  TITLE     Role of deoxycytidine kinase in an in vitro model for AraC- and
            DAC-resistance: substrate-enzyme interactions with deoxycytidine,
            1-beta-D-arabinofuranosylcytosine and 5-aza-2'-deoxycytidine
  JOURNAL   Leukemia 7 (7), 1005-1011 (1993)
   PUBMED   7686601
REFERENCE   7  (bases 1 to 4538)
  AUTHORS   Singhal,R.L. and Weber,G.
  TITLE     Deoxycytidine kinase and thymidine kinase activities in rat brain
  JOURNAL   Oncol Res 5 (4-5), 191-195 (1993)
   PUBMED   8305745
REFERENCE   8  (bases 1 to 4538)
  AUTHORS   Datta,N.S., Shewach,D.S., Hurley,M.C., Mitchell,B.S. and Fox,I.H.
  TITLE     Human T-lymphoblast deoxycytidine kinase: purification and
            properties
  JOURNAL   Biochemistry 28 (1), 114-123 (1989)
   PUBMED   2539852
REFERENCE   9  (bases 1 to 4538)
  AUTHORS   Bohman,C. and Eriksson,S.
  TITLE     Deoxycytidine kinase from human leukemic spleen: preparation and
            characteristics of homogeneous enzyme
  JOURNAL   Biochemistry 27 (12), 4258-4265 (1988)
   PUBMED   2844225
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On Nov 24, 2020 this sequence version replaced NM_024158.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ212366.1, SRR26643298.53867.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN11044071,
                                           SAMN12840105 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-425               JAXUCZ010000014.1  19609902-19610326   c
            426-541             JAXUCZ010000014.1  19605641-19605756   c
            542-735             JAXUCZ010000014.1  19601828-19602021   c
            736-883             JAXUCZ010000014.1  19600755-19600902   c
            884-999             JAXUCZ010000014.1  19597442-19597557   c
            1000-1090           JAXUCZ010000014.1  19595974-19596064   c
            1091-4538           JAXUCZ010000014.1  19589298-19592745   c
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p22"
     gene            1..4538
                     /gene="Dck"
                     /note="deoxycytidine kinase"
                     /db_xref="GeneID:79127"
                     /db_xref="RGD:620667"
     exon            1..425
                     /gene="Dck"
                     /inference="alignment:Splign:2.1.0"
     CDS             335..1117
                     /gene="Dck"
                     /EC_number="2.7.1.74"
                     /EC_number="2.7.1.113"
                     /EC_number="2.7.1.76"
                     /note="deoxyadenosine kinase; deoxyguanosine kinase"
                     /codon_start=1
                     /product="deoxycytidine kinase"
                     /protein_id="NP_077072.2"
                     /db_xref="GeneID:79127"
                     /db_xref="RGD:620667"
                     /translation="MATPPKRFCSSPSTSSEGTRIKKISIEGNIAAGKSTFVNILKQV
                     CEDWEVVPEPVARWCNVQSTQDEFEELTTSQKSGGNVLQMMYEKPERWSFIFQSYACL
                     SRIRAQLASLNGRLRDAEKPVLFFERSVYSDRYIFASNLYESDCMNETEWTIYQDWHD
                     WMNSQFGQSLELDGIIYLRATPEKCLNRIYIRGRDEEQGIPLEYLEKLHYKHESWLLH
                     RTLKTNFEYLQEVPILTLDVNEDFKDKHESLVEKVKEFLSTL"
     exon            426..541
                     /gene="Dck"
                     /inference="alignment:Splign:2.1.0"
     exon            542..735
                     /gene="Dck"
                     /inference="alignment:Splign:2.1.0"
     exon            736..883
                     /gene="Dck"
                     /inference="alignment:Splign:2.1.0"
     exon            884..999
                     /gene="Dck"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1090
                     /gene="Dck"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..4538
                     /gene="Dck"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgcgaggcc cgcccccgcc ggctcacagc tcgtcgcgca gggtcagtcg cctggctctc
       61 cgggttcgct tcacaagtcc cgccccctgc cctcctctcc cggggcgcct cgccctcccg
      121 gtggagttgc gcactgtgca gagctcgcga gggcaaggct tggagggtgg gagtcggggc
      181 tgccagggcg gggtccttcg caggcccgcc cagtcgcctg cgcctccgcg cgccaaagtc
      241 aaagtgcggg tcccgccggc gcgagattga cgctgcaacc ccgagctacc gacgccgccg
      301 ccgccgcctt cctgcaggtc ccgcgtcctt aggcatggcc accccaccta agaggttctg
      361 ctcttctccc tcaaccagct ctgagggcac ccgcatcaag aagatctcca tcgaagggaa
      421 catcgctgcc gggaagtcaa cgtttgtgaa tatcttaaag caagtctgtg aggattggga
      481 agtggttcct gagcctgtgg ccaggtggtg caatgtgcag agtacccaag atgagtttga
      541 ggagttgaca acatctcaga agagtggtgg gaatgttctt cagatgatgt atgagaaacc
      601 tgagcggtgg tcttttatct tccaatcata cgcctgtctc agccggatcc gggcccagct
      661 tgcctctctc aacggcaggc tcagggatgc agagaaacct gtattatttt ttgaacgatc
      721 tgtgtatagt gacaggtata ttttcgcatc taatttgtat gaatctgact gcatgaatga
      781 aacagaatgg acaatatatc aagactggca tgactggatg aatagccagt ttggccaaag
      841 ccttgaattg gatggaataa tttatcttcg agctactcca gagaagtgct taaatagaat
      901 atatatacgg ggaagggatg aagagcaagg cattcctctt gaatatttag agaaacttca
      961 ctataaacac gaaagctggc tccttcatcg gactctgaaa accaactttg aatatctcca
     1021 agaggtgcct atcctcacat tggatgttaa tgaagacttt aaagacaaac acgaaagtct
     1081 ggttgaaaag gtcaaagaat ttttgagtac tttgtgattg cacaggagac aacaggcaac
     1141 aaaatgtttc tacatacttc agttttgtat attgccatag cttgatatta ctgcaaaagt
     1201 ctttagaaga ttaaagtttt taaaccattt ttgttttaag aaaaacccct ttcctttata
     1261 acatgtatat atatatagtt tatttaatat ttgtaatcgt atgtaaaact ttgtgtcctt
     1321 cctttactcc ctacatcctc tgcttctggt cgtccttcct ttaggtttta aatcagaggt
     1381 gtgtaatctc tcacaacagg aagtacggaa gtagattttc ctagaatcag tgcagtgaat
     1441 ggataaactg ttctaacagg cccagtcttc tctgccttct cagttgtcat cctgagcagg
     1501 ttaacgtgtt ttcatggtgt tcagtggcga gcagtttcat aactggacac tttaccatga
     1561 ggatgcatat cttatgtaag gtagtgtacc tgttaaaagc tgagaagtgg cgtttgagga
     1621 gtccttgggg tagggatgtc acagcgtttg cgttgttttc ttcctgttcc ctttggtcga
     1681 gctggagatg gaagccctac agagctgtcc ctagcccttg atgttttcat caagtcctct
     1741 cctgctctgc tcgatgtgag ggtgagacca tgttagcagc accacagtga tgttcactgc
     1801 tgtggctggt gcgtttgtag aggaggaggt taaactcttt ccagtttgat aaatgtataa
     1861 ttactttata agtctcgtaa tctttaatct gtttcttttt tttttttagc atcttttttt
     1921 ttttttaaga tttattcatt tattatatat aagtacactg tagctgtcct cacacacacc
     1981 agaagagggc atcggatccc attacagatg gttgtgagcc accatgtggt tgctgggaat
     2041 ataactcagg acctctggaa gagcagtcag tgctcttaac cgctgagcca tctctccagc
     2101 cctttaatct gtttctttgt ctgggaagag tattctttat ttgcatgaaa ttttgcatca
     2161 gaagagatct ctacttaaga ggtagctaaa gtttattaca tgagtctaaa gcttcattaa
     2221 ggtagctgtt gcttgtttga attatgtttt ttgttttttt ttttttttaa gacagggtct
     2281 ctacatatcc ctagctgtcc tggagctcac tatatagacc aggctggcct ggccttgaat
     2341 ttacagaggt ccacctactt ctgcttccct ccccactccc agtgctcaga ctaaaggtgt
     2401 gcagtagcac actgggctct gatttttttg aaattatttg aggtaacctt cctcactaat
     2461 tttcttttag ttctcctgta aaatgatcat agaaggtttt cctgtaaaat gatcacaagg
     2521 ttttccattt ttccacagcc ctgagttgat aacgggtggt ctctctttag ctttggacat
     2581 ggtgagacac tcagaggcct aggttagctt ttgagcctcc cagtctgttc agccccacct
     2641 ctgataggca ggctgctgac ctgagattta aaccccattt ctgctgagag tgctgtggtc
     2701 acactaggct tcatagtttg cttgctttgt agaatttttc ttaaaagtag tcacagagaa
     2761 attactgata aaacaacttt tctttatata tagtgcatca tggaaatgta acaattttgt
     2821 tgaactaggt aataaaatta acaaagacca ggaggcctaa atctcccact aagaatgcac
     2881 gtacctctga actactttta tgtttagttg taagtatgct tggattcaac ttacgcctac
     2941 atagaattaa ccaagttttt tttttttttt aattgtttgt ttgctgtagt ttttaaagtt
     3001 aaaatgtgtg tcatttttac caagtaatta acctttctaa ttcatattaa gaaaaatgta
     3061 tgctggggtt ggggatttag ctcggtggta gagcgcttgc ctaggaagcg caaggccctg
     3121 ggttcggtcc ccagctctga aaaaaagaac caaaaaaaaa aaaaatgtat gctaaatgta
     3181 aataagaaat actcaagtta ataaaaaaaa aaagagaaat gtatgctttc ttgaaatgaa
     3241 ttgcatttct tttgaagcta taatttttct ttctaaaatg ttaccttttc tttttggttt
     3301 cttattcaaa gatgataatt cagtgggcca gcactgatct ttgaaaagag gaatcaattt
     3361 caagtcggta ataactgtag cttattttgt gatacttgtt ttttgttgtt gtttgatata
     3421 aatacatttg tccactcaaa tatttgtcaa taaatgaatt taaagttctt gagccatatg
     3481 gggagagttt taattttcca aagcaggcgt catttagttt tcttcttgga attttccaac
     3541 tttgtatttt ccacttaggt ctatgatcca ttttcagtgt tgtgacgata atcaggtttg
     3601 ttgtggattt gtgtgtgtgt acacacgtgc actgtatgcc tgttccaata ccacctttgc
     3661 ttccttttat ggctttgctc agctttttgg agactatctt tatgcaagcc tttccctagt
     3721 ttcttgacgt tggttatttt attcatcttt tttttttttt tttttttaac aatccagact
     3781 ttatcccctt cctggtccat cctctgactg ttctacccca tcccatacct cctcctccat
     3841 ctacaagagg atgtctcccc cttccccgcc agttgtagtt ttatagtaat ccagttgact
     3901 ggtgccatcc ttccatttgc ctgtttacaa gtcttggcct ggaactcagt cctgctccag
     3961 tgtccttggt gctggtttat agctgtgcac cacatggtca gcttcttttc agtgttgagc
     4021 tgggcaacgc tgagaccttt cgtcccctat ggaaatgtta gaatcggttt gccagtagcc
     4081 acaagagaac ttgctgacag cgccgattgg catgttgttg ggtctataga tcgagttggg
     4141 aagctctgac atttggatac tcatggagct ctgtcatttt gctgtcagtg aacatggatg
     4201 ggtatcattt catttagttc actgacagct tcaaagttgt gtctttttta tcatacagat
     4261 cgcatagatc ttctatgtgt ttttgtttta cgcctaagtg gtaatgcgtt tgctatgtta
     4321 atgtattcta agtttttgtt catttctgtt gttcttccgc cttaatgatc ttgctgtgga
     4381 cagagacttt ctctgtcatt gttcccaaca aaattgcctt ctctattctt tttattactt
     4441 taggtaggac tttgagaggg gattgtcgaa ccttgttccc ggtcttacca gggaagcttt
     4501 agttttctca tattaaagta atgttaattg ttccagct
//
