LOCUS       NM_053312               3166 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus dihydrolipoamide branched chain transacylase E2
            (Dbt), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_053312 XM_001073131 XM_342328
VERSION     NM_053312.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Davison,E.J., Pennington,K., Hung,C.C., Peng,J., Rafiq,R.,
            Ostareck-Lederer,A., Ostareck,D.H., Ardley,H.C., Banks,R.E. and
            Robinson,P.A.
  TITLE     Proteomic analysis of increased Parkin expression and its
            interactants provides evidence for a role in modulation of
            mitochondrial function
  JOURNAL   Proteomics 9 (18), 4284-4297 (2009)
   PUBMED   19725078
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Bogenhagen,D.F., Rousseau,D. and Burke,S.
  TITLE     The layered structure of human mitochondrial DNA nucleoids
  JOURNAL   J Biol Chem 283 (6), 3665-3675 (2008)
   PUBMED   18063578
REFERENCE   3  (bases 1 to 3166)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   4  (bases 1 to 3166)
  AUTHORS   Ono,K., Hakozaki,M., Suzuki,T., Mori,T., Hata,H. and Kochi,H.
  TITLE     cDNA cloning of the chicken branched-chain alpha-keto acid
            dehydrogenase complex. Chicken-specific residues of the
            acyltransferase affect the overall activity and the interaction
            with the dehydrogenase
  JOURNAL   Eur J Biochem 268 (3), 727-736 (2001)
   PUBMED   11168412
REFERENCE   5  (bases 1 to 3166)
  AUTHORS   Lebo,R.V., Shapiro,L.R., Fenerci,E.Y., Hoover,J.M., Chuang,J.L.,
            Chuang,D.T. and Kronn,D.F.
  TITLE     Rare etiology of autosomal recessive disease in a child with
            noncarrier parents
  JOURNAL   Am J Hum Genet 67 (3), 750-754 (2000)
   PUBMED   10915611
REFERENCE   6  (bases 1 to 3166)
  AUTHORS   Lau,K.S., Herring,W.J., Chuang,J.L., McKean,M., Danner,D.J.,
            Cox,R.P. and Chuang,D.T.
  TITLE     Structure of the gene encoding dihydrolipoyl transacylase (E2)
            component of human branched chain alpha-keto acid dehydrogenase
            complex and characterization of an E2 pseudogene
  JOURNAL   J Biol Chem 267 (33), 24090-24096 (1992)
   PUBMED   1429740
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC101904.1, CK356543.1, AB047915.1, BF547307.1, BM391990.1 and
            AA955113.1.
            
            On or before Oct 28, 2007 this sequence version replaced
            XM_342328.3, XM_001073131.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC166487.1, SRR8487231.43370.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132266, SAMD00132270
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BC101904.1         1-14
            15-715              CK356543.1         1-701
            716-801             AB047915.1         474-559
            802-2345            BC101904.1         1014-2557
            2346-2570           BF547307.1         23-247              c
            2571-3036           BM391990.1         135-600             c
            3037-3166           AA955113.1         1-130               c
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q42"
     gene            1..3166
                     /gene="Dbt"
                     /note="dihydrolipoamide branched chain transacylase E2"
                     /db_xref="GeneID:29611"
                     /db_xref="RGD:68403"
     exon            1..69
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..1467
                     /gene="Dbt"
                     /EC_number="2.3.1.168"
                     /codon_start=1
                     /product="lipoamide acyltransferase component of
                     branched-chain alpha-keto acid dehydrogenase complex,
                     mitochondrial"
                     /protein_id="NP_445764.1"
                     /db_xref="GeneID:29611"
                     /db_xref="RGD:68403"
                     /translation="MAAARVLGTWSRNAVRLTCVRYFQTYNNVHILKPQHVCSVGYPL
                     LKSSQPRHSLRTAAVLQGQVVQFKLSDIGEGIREVTIKEWYVKEGDTVSQFDSICEVQ
                     SDKASVTITSRYDGVIKRLYYNLDDIAYVGKPLIDIETEALKDSEEDVVETPAVAHDE
                     HTHQEIKGQKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNFLEKQTGAILP
                     PSPKSEITPPPPQPRDRPFPTPVSKPPVFLGKDRTEPVTGFQKAMVKTMSAALKIPHF
                     GYCDEVDLTELVKLREELKPVALARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQ
                     SITYKASHNIGIAMDTERGLIVPNVKNVQVRSVFEIAMELNRLQKLGSLGQLSTTDLT
                     GGTFTLSNIGSIGGTYAKPVILPPEVAIGALGAIKALPRFDQKGDVYKAQIMNVSWSA
                     DHRVIDGATMSRFSNLWKSYLENPAFMLLDLK"
     exon            70..193
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            194..269
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            270..451
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            452..573
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            574..790
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            791..957
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1035
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1227
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1299
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..3152
                     /gene="Dbt"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctatttcc ggagtaagat ggctgcagcg cgtgtgctgg ggacctggag ccggaacgct
       61 gtcagattga cctgtgttcg ctattttcaa acatataata acgtacacat tttgaagcca
      121 caacacgtgt gctctgtggg ttatccattg ctcaagtcca gtcagccacg tcactccctg
      181 agaacagcag ccgtgctcca gggacaggtt gttcaattca aactctccga catcggagaa
      241 gggattcgag aagtaaccat taaagaatgg tatgtaaaag aaggagatac ggtgtctcag
      301 tttgacagca tctgtgaagt tcaaagtgac aaggcctctg tcaccatcac cagccgttac
      361 gatggcgtca ttaagaggct ctattacaat ctggacgata tcgcttatgt ggggaaacca
      421 ctgatagaca tcgagacaga agctttaaag gattcagaag aagatgtggt cgaaactcct
      481 gctgtagccc atgatgaaca cacccaccaa gagataaaag gccagaaaac actagcaacg
      541 cctgcagttc gtcggctggc aatggaaaac aatatcaagc tgagtgaagt tgttggctca
      601 ggaaaggatg gcagaatact taaagaagac attctcaact ttttggaaaa gcagacagga
      661 gccatactgc ctccctcacc gaaatcggaa atcacaccac cgccaccaca gcccagagac
      721 aggccctttc ccacgccggt atcaaagcct ccagtattcc tgggcaaaga ccgaacagag
      781 cctgtgacag gcttccaaaa ggcaatggtc aagaccatgt ccgcagccct gaagattcct
      841 cactttgggt actgtgatga ggttgacctt actgaactgg ttaagcttcg agaagaattg
      901 aaacctgttg ctttggctcg tgggatcaaa ctctccttca tgccgttctt cttaaaggct
      961 gcgtctttgg gactactgca gttccctatc cttaatgcct ccgtggatga aaactgccag
     1021 agcatcacct acaaggcctc tcacaacatt gggatagcca tggataccga gcgggggtta
     1081 attgtcccca acgtgaagaa cgttcaggtg cgctctgtat ttgagatcgc catggaactg
     1141 aaccgcctcc agaaactggg ctctttgggt cagctcagta ccacagacct cacgggaggg
     1201 acattcactc tttccaacat tggatcaatt ggtggaacct atgccaagcc agtgatattg
     1261 ccacctgaag tagccattgg cgccctggga gccattaagg ctcttccccg atttgaccag
     1321 aaaggggatg tttacaaagc ccagataatg aatgtgagct ggtcagcaga tcacagggtc
     1381 attgacggcg ccaccatgtc acgattctct aacttgtgga aatcctactt agaaaaccca
     1441 gcgttcatgc tgctggatct gaaataacgg cgaaccagac atccttgaac ttttcgcact
     1501 tccaaagagt atgtcaggcc cggctgagcc agcaccgact cttgttacag ttgcattaga
     1561 ggtggcttgt gctgccttgg tagggctcta acgcacagga tttatcactg acgtattagg
     1621 ctttctgctg aaaaggagcg gagggtaacg gttctcctgg ggtatcacac tgtgtaggtc
     1681 acgagggggc ttcccactat ggtgctgaga tcattgggtc agtgagttga aacaagcaac
     1741 aaaacaaaat cgacatcact caatgtcact aataatgtca ctcaatggtg aatagcagtg
     1801 cagatgctgc acttgctctc tttttaattt gtgactgatt ttttaatgaa acttttaaat
     1861 tataattaat cggggaaagg aatttatata caaatatgtt ttcatttccc aacaatcaag
     1921 gcgactgccc tataaaataa gtggaattat gtttatttct gcagctaatg agacatatac
     1981 tcttgcctta tgtgtaaact atcatctata aatgtcttat agctataaat taggatttgt
     2041 taaaagggga agttataatt tggtcttaat tatagtttac aaccaaaaag gctgttaaaa
     2101 gtaaatgttt aaaatggata gacttctaga gctgtagaaa tgctttggca tcttgctggg
     2161 aaatacttga ctttgcaaac tcactttgtg atgtaaaata ccataaatac agcatcactg
     2221 gctatttttc tcttactact taccgattta ttgccttttg atttcattga tcttcagcta
     2281 aacatgagaa aaggacttgg aatctgttct gttctcccgt ccctcagtag gagcatgaat
     2341 aagaagagtc aaatgggaaa ttatgggcac acaattagtt tatacagaaa tgctacttta
     2401 atccgatagc gtcaggatga atcgaattca aggctgagca aacctttatt cccagcactt
     2461 gagaagcaaa gacaagtgga tctctgtgag tttgaggcca gcctgctcta ccgagtaagt
     2521 tctcagacag ccagaggtac atagagagac cctgtattaa aaaaaaaaaa atcaaataaa
     2581 atcaaattca gtattttgaa atctaggttt ggggtttttt cttctaatat tatctaatta
     2641 aatatatcat gacaatatcg cttatttaaa tgaaagtttt gtggcagaag aaaaacgggt
     2701 cattttaatg cctacttgat tttttcccaa aatggaatta tgttataaat ttagtgagta
     2761 ctgtttggtt agaaattcac atcacgaaac ccattgtttt cacataaaga aaattaagaa
     2821 aagagaacca attcagaaca ggcattgtgt ccgtaggcct gtaatcccca tacctgggag
     2881 ggaggcaaag gcaagaagga ccatggcagg aggccatttt aggtcatgta gtgagacttt
     2941 gttggaagaa ggagaaacta tagatagcaa ggagagtagc acactgctat ctataatcta
     3001 aacattatta acatgatata tgtgtctgta tacatatata agtattcctt tataaaaatg
     3061 aaataatata tctactattt tacaatttga ttttttaaaa tttatcctta tggatgtctt
     3121 tatatgccaa taaacagttc cacattatca taaaaaaaaa aaaaaa
//
