LOCUS       NM_001034143            2321 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus aspartyl-tRNA synthetase 2 (mitochondrial)
            (Dars2), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001034143 XM_222804
VERSION     NM_001034143.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Neuenfeldt,A., Lorber,B., Ennifar,E., Gaudry,A., Sauter,C.,
            Sissler,M. and Florentz,C.
  TITLE     Thermodynamic properties distinguish human mitochondrial
            aspartyl-tRNA synthetase from bacterial homolog with same 3D
            architecture
  JOURNAL   Nucleic Acids Res 41 (4), 2698-2708 (2013)
   PUBMED   23275545
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Scheper,G.C., van der Klok,T., van Andel,R.J., van Berkel,C.G.,
            Sissler,M., Smet,J., Muravina,T.I., Serkov,S.V., Uziel,G.,
            Bugiani,M., Schiffmann,R., Krageloh-Mann,I., Smeitink,J.A.,
            Florentz,C., Van Coster,R., Pronk,J.C. and van der Knaap,M.S.
  TITLE     Mitochondrial aspartyl-tRNA synthetase deficiency causes
            leukoencephalopathy with brain stem and spinal cord involvement and
            lactate elevation
  JOURNAL   Nat Genet 39 (4), 534-539 (2007)
   PUBMED   17384640
REFERENCE   3  (bases 1 to 2321)
  AUTHORS   Bonnefond,L., Fender,A., Rudinger-Thirion,J., Giege,R., Florentz,C.
            and Sissler,M.
  TITLE     Toward the full set of human mitochondrial aminoacyl-tRNA
            synthetases: characterization of AspRS and TyrRS
  JOURNAL   Biochemistry 44 (12), 4805-4816 (2005)
   PUBMED   15779907
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC105774.1.
            
            [WARNING] On May 3, 2024 this sequence was replaced by
            NM_001034143.2.
            
            On Oct 14, 2005 this sequence version replaced XM_222804.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC105774.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q22"
     gene            1..2321
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /note="aspartyl-tRNA synthetase 2 (mitochondrial)"
                     /db_xref="GeneID:304919"
                     /db_xref="RGD:1308286"
     exon            1..231
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..2066
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /EC_number="6.1.1.12"
                     /note="aspartyl-tRNA synthetase, mitochondrial; aspRS;
                     aspartate--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="aspartate--tRNA ligase, mitochondrial precursor"
                     /protein_id="NP_001029315.1"
                     /db_xref="GeneID:304919"
                     /db_xref="RGD:1308286"
                     /translation="MYLGSWLNRLGRGLSRPIGKTKQPIWGSLSRSLTLSSQRVPEFS
                     SFVARTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDCHGLVQILIPQDESAASV
                     RRTLCEAPVESVVRVSGTVIARPLGQENPKMPTGEIEIKAKTAELLNACKKLPFEIKD
                     FVKKTEALRLQYRYLDLRSSQMQHNLRLRSQMVMKMREYLCTLHGFVDIETPTLFKRT
                     PGGAKEFLVPSREPGRFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPE
                     FTQIDIEMSFVDQTGIQHLVEGLLHYSWPEDKDPLVAPFPSMTFAEALATYGTDKPDT
                     RFGMKIVDISDVFRNTEIRFLQDALAKPQGTVKAICVHEGAKYLRKEDIEFIRKFAAH
                     HFSQEVLPIFLNARKNWSSPFAKFITEEERLELTRLMEIQEDDMVLLTAGQHEKACSL
                     LGKLRLECADLLETRGLALRDPALFSFLWVLDFPLFLAKEESPTELESAHHPFTAPHP
                     GDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSIRIHDAQLQRYILETLLKEDVKLLSHL
                     LQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGHDLMSNAPDTVSPED
                     LKPYHIHVSWPTDSEERASATPSKYLSS"
     exon            232..331
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            332..398
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            399..500
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            501..596
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            597..720
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            721..767
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            768..874
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            875..944
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1124
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1232
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1295
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1448
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1667
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1778
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1854
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..2283
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcctggatg catttgcgtg ggaagtagaa ttttaagggt tccctggtat tccaaagccg
       61 agttttgagc accaccacac ctgcagcgcg gattccggga cttcaacatg tatcttggtt
      121 cttggttaaa tcgtctaggc aggggtttat ccagacccat cggaaagacc aagcagccaa
      181 tctggggttc tctttccaga agtctgacgt tgagttcaca gagagttcca gaattcagta
      241 gctttgttgc tcggaccaac acatgtggag aacttcgttc ttcccactta ggccaagaag
      301 tcactctgtg tggatggatt cagtaccgaa ggcaaaacac cttcttagtc ctaagagatt
      361 gccacggtct cgttcaaatc ctcattcccc aggatgagtc ggctgcatct gtgaggagga
      421 ctttgtgtga agcccctgtg gagtctgtgg tgagagtatc tgggacggtc atcgcccgac
      481 ccctgggaca ggagaatccg aaaatgccaa caggtgagat tgaaatcaaa gctaaaacag
      541 ctgagctcct gaatgcctgc aaaaagctgc cctttgaaat taaagacttt gtgaagaaga
      601 cggaagccct tcgtctgcag tatcggtact tagacctgcg cagctcccaa atgcagcaca
      661 acctgcggct caggtcacag atggtcatga aaatgcggga atatctctgt actctgcacg
      721 gatttgtgga tatagaaacc ccaacattgt ttaagaggac tcctggaggt gccaaggagt
      781 ttttagtacc gtccagggaa cctggaaggt tttattctct ccctcagagt cctcaacagt
      841 ttaagcagct tctgatggtt ggtggtttag acaggtactt tcaggttgcc cggtgttatc
      901 gagatgaagg ctcaagaccg gataggcagc cagagtttac tcagattgac atagagatgt
      961 cctttgtaga ccagactggc attcagcatt tagttgaggg tttgctgcat tattcctggc
     1021 ctgaagataa agaccctcta gtggctcctt ttccttccat gacttttgct gaggcattgg
     1081 ctacctacgg aactgataag ccagatactc gctttgggat gaagattgtg gatatcagtg
     1141 atgtgttcag aaacacagag atcaggtttc ttcaggatgc actggctaag ccccaaggaa
     1201 ctgtcaaagc aatatgtgtt catgaaggag caaagtactt aaggaaagaa gacattgagt
     1261 tcattcgaaa gtttgcagct caccatttca gtcaggaagt ccttcctata ttcctgaatg
     1321 ccaggaagaa ctggagttca ccatttgcta aattcataac ggaggaggaa agattggagt
     1381 taaccagatt gatggagata caagaagacg acatggtgct gctgactgct gggcaacatg
     1441 agaaagcatg ctctttgttg gggaagttac gactagagtg tgcagacctt ttggaaacga
     1501 gaggactggc gctcagggac ccagcattgt tctcttttct ctgggtactt gacttcccac
     1561 tcttcctcgc caaggaagag agtcccacag agctggagtc agcccaccac ccatttactg
     1621 ctcctcaccc cggcgacatt cacctcctct acactgagcc ggaaaaggtt cgtggccaac
     1681 actacgactt ggttttaaat ggcaatgaga taggaggagg ctctatccgc attcatgatg
     1741 cacagctaca gaggtacatc ctggaaacat tgctaaagga agatgtgaaa ttgctttccc
     1801 atctgcttca ggctttagac tatggggcac ccccacatgg aggaattgcc ttagggttag
     1861 acagactggt gtgtcttgtc acgggagcac ccagcatcag agatgtcata gccttcccta
     1921 aatcctaccg aggacatgac cttatgagca atgctccaga cactgtgtct cctgaagatc
     1981 tgaagcctta ccatatccac gtctcatggc caacagactc agaagagaga gcatccgcca
     2041 caccttccaa atacttgagc tcttgacttg tcttctttgc tttactaaac ctaaaaagaa
     2101 gatccagact tgccacagac tttagaatca agtttctgtg tagaagcaat cattaccgag
     2161 taagaaacag aagctacaca attgtggtca tatgtaccat taggactttc tgtagttggg
     2221 agttctgtgg aagtttttta attggagaat tgtaaagtga aacaaaaaaa gctttttatt
     2281 ggaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//
