LOCUS       NP_446251                501 aa            linear   ROD 01-JUN-2024
DEFINITION  aspartate--tRNA ligase, cytoplasmic [Rattus norvegicus].
ACCESSION   NP_446251
VERSION     NP_446251.1
DBSOURCE    REFSEQ: accession NM_053799.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 501)
  AUTHORS   Frohlich,D., Suchowerska,A.K., Spencer,Z.H., von Jonquieres,G.,
            Klugmann,C.B., Bongers,A., Delerue,F., Stefen,H., Ittner,L.M.,
            Fath,T., Housley,G.D. and Klugmann,M.
  TITLE     In vivocharacterization of the aspartyl-tRNA synthetase DARS:
            Homing in on the leukodystrophy HBSL
  JOURNAL   Neurobiol Dis 97 (Pt A), 24-35 (2017)
   PUBMED   27816769
REFERENCE   2  (residues 1 to 501)
  AUTHORS   Topalidou,I., Cattin-Ortola,J., Pappas,A.L., Cooper,K.,
            Merrihew,G.E., MacCoss,M.J. and Ailion,M.
  TITLE     The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo
            Sorting to Dense-Core Vesicles
  JOURNAL   PLoS Genet 12 (5), e1006074 (2016)
   PUBMED   27191843
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 501)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (residues 1 to 501)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   5  (residues 1 to 501)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (residues 1 to 501)
  AUTHORS   Kaminska,M., Havrylenko,S., Decottignies,P., Gillet,S., Le
            Marechal,P., Negrutskii,B. and Mirande,M.
  TITLE     Dissection of the structural organization of the aminoacyl-tRNA
            synthetase complex
  JOURNAL   J Biol Chem 284 (10), 6053-6060 (2009)
   PUBMED   19131329
REFERENCE   7  (residues 1 to 501)
  AUTHORS   Akiyama,K., Morita,H., Suetsugu,S., Kuraba,S., Numata,Y.,
            Yamamoto,Y., Inui,K., Ideura,T., Wakisaka,N., Nakano,K., Oniki,H.,
            Takenawa,T., Matsuyama,M. and Yoshimura,A.
  TITLE     Actin -related protein 3 (Arp3) is mutated in proteinuric BUF/Mna
            rats
  JOURNAL   Mamm Genome 19 (1), 41-50 (2008)
   PUBMED   18064521
REFERENCE   8  (residues 1 to 501)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   9  (residues 1 to 501)
  AUTHORS   Lazard,M., Agou,F., Cavarelli,J., Latreille,M.T., Moras,D. and
            Mirande,M.
  TITLE     Genomic organization of the rat aspartyl-tRNA synthetase gene
            family: a single active gene and several retropseudogenes
  JOURNAL   Gene 180 (1-2), 197-205 (1996)
   PUBMED   8973367
REFERENCE   10 (residues 1 to 501)
  AUTHORS   Mirande,M. and Waller,J.P.
  TITLE     Molecular cloning and primary structure of cDNA encoding the
            catalytic domain of rat liver aspartyl-tRNA synthetase
  JOURNAL   J Biol Chem 264 (2), 842-847 (1989)
   PUBMED   2642907
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC072534.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC072534.1, J04487.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q13"
     Protein         1..501
                     /product="aspartate--tRNA ligase, cytoplasmic"
                     /EC_number="6.1.1.12"
                     /note="aspartyl-tRNA synthetase, cytoplasmic"
                     /calculated_mol_wt=56995
     Region          20..501
                     /region_name="PLN02850"
                     /note="aspartate-tRNA ligase"
                     /db_xref="CDD:215456"
     Site            52
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P14868; propagated from
                     UniProtKB/Swiss-Prot (P15178.1)"
     Site            74
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P14868; propagated from
                     UniProtKB/Swiss-Prot (P15178.1)"
     Site            249
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P14868; propagated from
                     UniProtKB/Swiss-Prot (P15178.1)"
     Region          251..254
                     /region_name="Aspartate. /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (P15178.1)"
     Site            374
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P14868; propagated from
                     UniProtKB/Swiss-Prot (P15178.1)"
     CDS             1..501
                     /gene="Dars1"
                     /gene_synonym="aspRS; Dars; DRS1"
                     /coded_by="NM_053799.3:126..1631"
                     /db_xref="GeneID:116483"
                     /db_xref="RGD:621167"
ORIGIN      
        1 mpsanasrkg qekpreivda aedyakeryg vssmiqsqek pdrvlvrvkd ltvqkadevv
       61 wvrarvhtsr akgkqcflvl rqqqfnvqal vavgdhaskq mvkfaanink esiidvegiv
      121 rkvnqkigsc tqqdvelhvq kiyvislaep rlplqlddai rpevegeedg ratvnqdtrl
      181 dnriidlrts tsqaifhlqs gichlfretl inkgfveiqt pkiisaaseg ganvftvsyf
      241 ksnaylaqsp qlykqmcica dfekvfcigp vfraedsnth rhltefvgld iemafnyhyh
      301 evveeiadtl vqifkglqer fqteiqtvnk qfpcepfkfl eptlrleyce alamlreagv
      361 emddeedlst pnekllgrlv kekydtdfyv ldkyplavrp fytmpdprnp kqsnsydmfm
      421 rgeeilsgaq rihdpqllte ralhhgidle kikayidsfr fgapphaggg iglervtmlf
      481 lglhnvrqts mfprdpkrlt p
//
