LOCUS       NM_019138               2626 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Rattus norvegicus cytochrome P450 family 7 subfamily B member 1
            (Cyp7b1), mRNA.
ACCESSION   NM_019138 XM_001060253 XM_342218
VERSION     NM_019138.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Wicher,G. and Norlin,M.
  TITLE     Estrogen-mediated regulation of steroid metabolism in rat glial
            cells; effects on neurosteroid levels via regulation of
            CYP7B1-mediated catalysis
  JOURNAL   J Steroid Biochem Mol Biol 145, 21-27 (2015)
   PUBMED   25263657
  REMARK    GeneRIF: estrogen suppresses CYP7B1-catalyzed 7alpha-hydroxylation
            of DEHA
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Yi,T., Wang,X., Kelly,L.M., An,J., Xu,Y., Sailer,A.W.,
            Gustafsson,J.A., Russell,D.W. and Cyster,J.G.
  TITLE     Oxysterol gradient generation by lymphoid stromal cells guides
            activated B cell movement during humoral responses
  JOURNAL   Immunity 37 (3), 535-548 (2012)
   PUBMED   22999953
REFERENCE   3  (bases 1 to 2626)
  AUTHORS   Noreau,A., Dion,P.A., Szuto,A., Levert,A., Thibodeau,P., Brais,B.,
            Dupre,N., Rioux,M.F. and Rouleau,G.A.
  TITLE     CYP7B1 mutations in French-Canadian hereditary spastic paraplegia
            subjects
  JOURNAL   Can J Neurol Sci 39 (1), 91-94 (2012)
   PUBMED   22384504
  REMARK    GeneRIF: This study deminitrated that CYP7B1 mutations in patients
            with hereditary spastic paraplegia in French-Canadian.
REFERENCE   4  (bases 1 to 2626)
  AUTHORS   Shi,Q.Y., Lin,Y.G., Zhou,X., Lin,Y.Q. and Yan,S.
  TITLE     [Interaction among peroxisome proliferators-activated receptor
            alpha, cytochrome P450 oxysterol 7alpha-hydroxylase and estrogen
            receptor and its association with intrahepatic cholestasis in
            pregnant rats]
  JOURNAL   Zhonghua Fu Chan Ke Za Zhi 45 (6), 440-444 (2010)
   PUBMED   21029595
  REMARK    GeneRIF: Abnormal expression of PPARalpha, CYP7B1 and ER may play a
            role in the pathogenesis of estrogen-induced intrahepatic
            cholestasis.
REFERENCE   5  (bases 1 to 2626)
  AUTHORS   Galman,C., Bonde,Y., Matasconi,M., Angelin,B. and Rudling,M.
  TITLE     Dramatically increased intestinal absorption of cholesterol
            following hypophysectomy is normalized by thyroid hormone
  JOURNAL   Gastroenterology 134 (4), 1127-1136 (2008)
   PUBMED   18395092
  REMARK    GeneRIF: Hepatic secretion of cholesterol and ABCG5/G8 expression
            are strongly stimulated in hypophysectomized rats during treatment
            with thyroid hormone.
REFERENCE   6  (bases 1 to 2626)
  AUTHORS   Weihua,Z., Lathe,R., Warner,M. and Gustafsson,J.A.
  TITLE     An endocrine pathway in the prostate, ERbeta, AR,
            5alpha-androstane-3beta,17beta-diol, and CYP7B1, regulates prostate
            growth
  JOURNAL   Proc Natl Acad Sci U S A 99 (21), 13589-13594 (2002)
   PUBMED   12370428
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2006 May 23;103(21):8298]
REFERENCE   7  (bases 1 to 2626)
  AUTHORS   Pandak,W.M., Hylemon,P.B., Ren,S., Marques,D., Gil,G., Redford,K.,
            Mallonee,D. and Vlahcevic,Z.R.
  TITLE     Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in primary
            cultures of rat hepatocytes
  JOURNAL   Hepatology 35 (6), 1400-1408 (2002)
   PUBMED   12029625
  REMARK    GeneRIF: CYP7B1 specific activity is highly regulated but does not
            seem to be rate limiting for bile acid synthesis.
REFERENCE   8  (bases 1 to 2626)
  AUTHORS   Laes,J.F., Ravoet,M., Quan,X., Van Vooren,P., Szpirer,J. and
            Szpirer,C.
  TITLE     Improved radiation hybrid map of rat chromosome 2: colocalization
            of the genes encoding corticotropin-releasing hormone and
            IL6-receptor with quantitative trait loci regulating the
            inflammatory response
  JOURNAL   Cytogenet Cell Genet 92 (1-2), 130-133 (2001)
   PUBMED   11306811
REFERENCE   9  (bases 1 to 2626)
  AUTHORS   Li-Hawkins,J., Lund,E.G., Bronson,A.D. and Russell,D.W.
  TITLE     Expression cloning of an oxysterol 7alpha-hydroxylase selective for
            24-hydroxycholesterol
  JOURNAL   J Biol Chem 275 (22), 16543-16549 (2000)
   PUBMED   10748047
REFERENCE   10 (bases 1 to 2626)
  AUTHORS   Stapleton,G., Steel,M., Richardson,M., Mason,J.O., Rose,K.A.,
            Morris,R.G. and Lathe,R.
  TITLE     A novel cytochrome P450 expressed primarily in brain
  JOURNAL   J Biol Chem 270 (50), 29739-29745 (1995)
   PUBMED   8530364
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB800565.1, CB760136.1, U36992.1, BF283070.1 and CB749347.1.
            
            On or before Oct 13, 2007 this sequence version replaced
            XM_001060253.1, XM_342218.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5756307,
                              SAMEA5760400 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-341               CB800565.1         55-395
            342-464             CB760136.1         311-433
            465-1958            U36992.1           68-1561
            1959-1991           BF283070.1         579-611             c
            1992-2059           U36992.1           1595-1662
            2060-2240           BF283070.1         330-510             c
            2241-2626           CB749347.1         55-440
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="LE"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q24"
     gene            1..2626
                     /gene="Cyp7b1"
                     /note="cytochrome P450 family 7 subfamily B member 1"
                     /db_xref="GeneID:25429"
                     /db_xref="RGD:2483"
     exon            1..234
                     /gene="Cyp7b1"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..1642
                     /gene="Cyp7b1"
                     /EC_number="1.14.14.29"
                     /note="HCT-1; cytochrome P450 7B1; oxysterol
                     7-alpha-hydroxylase; hippocampal transcript 1 protein;
                     cytochrome P450, subfamily 7B, polypeptide 1; cytochrome
                     P450, family 7, subfamily b, polypeptide 1"
                     /codon_start=1
                     /product="25-hydroxycholesterol 7-alpha-hydroxylase"
                     /protein_id="NP_062011.1"
                     /db_xref="GeneID:25429"
                     /db_xref="RGD:2483"
                     /translation="MEGATTPDAASPGPLSLLGLLFAVTLLLPVLFLLTRRTRRPCEP
                     PLIKGWIPYLGMALKFWKDPLAFLQTLQRQCGDTFTVLLGGKYITFVLNPFQYQYVMK
                     NPKQLSFEKFSRRLSAKAFSVKKLLTNDDLSNDIHRGYLLLQGKSLDGLLETMIQEVK
                     EIFESRLLKLTDWNTARVFDFCSSLVFEITFTTIYGKILAANKKQIISELRDDFLKFD
                     DHFPYLVSDIPIQLLRNAEFMQKKIIKCLTPEKVAQMQRRSEIVQERQEMLKKYYGHE
                     EFEIGAHHLGLLWASLANTIPAMFWAMYYLLQHPEAMEVLRDEIDSFLQSTGQKKGPG
                     ISVHFTREQLDSLVCLESAILEVLRLCSYSSIIREVQEDMDFSSESRSYRLRKGDFVA
                     VFPPMIHNDPEVFDAPKDFRFDRFVEDGKKKTTFFKGGKKLKSYIIPFGLGTSKCPGR
                     YFAINEMKLLVIILLTYFDLEVIDTKPIGLNHSRMFLGIQHPDSDISFRYKAKSWRS"
     exon            235..371
                     /gene="Cyp7b1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..962
                     /gene="Cyp7b1"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1169
                     /gene="Cyp7b1"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1345
                     /gene="Cyp7b1"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..2626
                     /gene="Cyp7b1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctttggggg ccgctgtgac attctgctct gcactgcggg caggccagag cctctggtct
       61 agaagagagg gcactttgca gaagccatcg ctcactacag agccgccaga gcgtccggat
      121 ggagggagcc acgaccccag atgccgcttc gcccgggcct ctctccctac taggccttct
      181 ctttgcggtc accttgctgc tcccagtcct gttcctcctc acccggcgca caaggcgtcc
      241 ttgtgaacct cccttgataa aaggttggat tccttatctt ggcatggccc tgaaattctg
      301 gaaggatccg ttagctttct tgcaaactct tcaaaggcaa tgtggtgaca ctttcactgt
      361 cttacttgga gggaagtata taacatttgt tctgaaccct ttccagtacc agtatgtaat
      421 gaaaaaccca aaacaattaa gctttgagaa gttcagccga agattatcag cgaaagcctt
      481 ctctgtcaag aagctgctaa ctaatgacga ccttagcaat gacattcaca gaggctatct
      541 tcttttacaa ggcaaatctc tggatggtct tctggaaacc atgatccaag aagtaaaaga
      601 aatatttgag tccagactgc taaaactcac agattggaat acagcaagag tatttgattt
      661 ctgtagttca ctggtatttg aaatcacatt tacaactata tatggaaaaa ttcttgctgc
      721 taacaaaaaa caaattatca gtgagctgag ggatgatttt ttaaaatttg atgaccattt
      781 cccatactta gtatctgaca tacctattca gcttctaaga aatgcagaat ttatgcagaa
      841 gaaaattata aaatgtctca caccagaaaa agtagctcag atgcaaagac ggtcagaaat
      901 tgttcaggag aggcaggaga tgctgaaaaa atactacggg catgaagagt ttgaaatagg
      961 agcacatcat cttggcttgc tctgggcctc tctagcaaac accattccag ctatgttctg
     1021 ggcaatgtat tatcttcttc agcatccaga agctatggaa gtcctgcgtg acgaaattga
     1081 cagcttcctg cagtcaacag gtcaaaagaa aggacctgga atttctgtcc acttcaccag
     1141 agaacaattg gacagcttgg tctgcctgga aagcgctatt cttgaggttc tgaggttgtg
     1201 ctcctactcc agcatcatcc gtgaagtgca agaggatatg gatttcagct cagagagtag
     1261 gagctaccgt ctgcggaaag gagactttgt agctgtcttt cctccaatga tacacaatga
     1321 cccagaagtc ttcgatgctc caaaggactt taggtttgat cgcttcgtag aagatggtaa
     1381 gaagaaaaca acgtttttca aaggaggaaa aaagctgaag agttacatta taccatttgg
     1441 acttggaaca agcaaatgtc caggcagata ctttgcaatt aatgaaatga agctactagt
     1501 gattatactt ttaacttatt ttgatttaga agtcattgac actaagccta taggactaaa
     1561 ccacagtcgc atgtttctgg gcattcagca tccagactct gacatctcat ttaggtacaa
     1621 ggcaaaatct tggagatcct gaaagggtgg cagagaagct tagcggaata aggctgcaca
     1681 tgctgagctc tgtgatttgc tgtactcccc aaatgcagcc actattcttg tttgttagaa
     1741 aatggcaaat ttttatttga ttgcgatcca tccagtttgt tttgggtcac aaaacctgtc
     1801 ataaaataaa gcgctgtcat ggtgtaaaaa aatgtcatgg caatcatttc aggataaggt
     1861 aaaataacgt tttcaagttt gtacttacta tgatttttat catttgtagt gaatgtgctt
     1921 ttccagtaat aaatttgcgc cagggtgatt ttttttaaat tactgaaatc ctctaatatg
     1981 gttttatgtg ctgccagaaa agtgtgccat caatggacag tataacaatt tccagttttc
     2041 cagagaaggg agaaattaaa ccccatgagt tacgctgtat aaaattgttc tcttcaacta
     2101 taatatcaat aatgtctata tcaccaggtt acctttgcat taaatgagtt ttgcaaaaga
     2161 ttaaatgtcc caacttcctt tcaatattta atcatccata aatgctcttt ataactttgt
     2221 attctacagc tcaatagaac acaattaatt gttatgtaag attgctcatg ttcaagttag
     2281 atattgttaa attttaatgt atatgaaaac acaaagccta ttccatagtc agcagttact
     2341 ctaaacatta gtattaggtt tattgtggag gtaaatgttg aagttacaat agcactgatg
     2401 gtagaattgg tctgtagtat aaactgtgtt agctgatgct gcaatgaacc ctttaccaaa
     2461 catttccatt tctataaaca gagaaattag gtagcattat ttatccacaa ccttttggac
     2521 ttgaagttaa gtttattatt ttaaactatt aaattaaact atacaaaata attctatgtg
     2581 ataccatata gcctgtattg attagtaaaa gatttctact atcaaa
//
