LOCUS       NM_012942               3544 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus cytochrome P450 family 7 subfamily A member 1
            (Cyp7a1), mRNA.
ACCESSION   NM_012942
VERSION     NM_012942.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Duan,Y., Zhang,F., Yuan,W., Wei,Y., Wei,M., Zhou,Y., Yang,Y.,
            Chang,Y. and Wu,X.
  TITLE     Hepatic cholesterol accumulation ascribed to the activation of
            ileum Fxr-Fgf15 pathway inhibiting hepatic Cyp7a1 in high-fat
            diet-induced obesity rats
  JOURNAL   Life Sci 232, 116638 (2019)
   PUBMED   31288013
  REMARK    GeneRIF: The increased CDCAs concentrations activated Fxr-Fgf15
            pathway in ileum to result in the mRNA expression of Cyp7a1 in
            liver down-regulation, which inhibited cholesterol metabolizing
            into primary BAs to contribute to the cholesterol level increase in
            liver tissue in HFD-induced obesity rats.
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Hao,C.L., Lin,H.L., Ke,L.Y., Yen,H.W. and Shen,K.P.
  TITLE     Pre-germinated brown rice extract ameliorates high-fat diet-induced
            metabolic syndrome
  JOURNAL   J Food Biochem 43 (3), e12769 (2019)
   PUBMED   31353547
REFERENCE   3  (bases 1 to 3544)
  AUTHORS   Hu,Y.B., Liu,X.Y. and Zhan,W.
  TITLE     Farnesoid X receptor agonist INT-767 attenuates liver steatosis and
            inflammation in rat model of nonalcoholic steatohepatitis
  JOURNAL   Drug Des Devel Ther 12, 2213-2221 (2018)
   PUBMED   30038487
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3544)
  AUTHORS   Huo,X.K., Liu,J., Yu,Z.L., Wang,Y.F., Wang,C., Tian,X.G., Ning,J.,
            Feng,L., Sun,C.P., Zhang,B.J. and Ma,X.C.
  TITLE     Alisma orientale extract exerts the reversing cholestasis effect by
            activation of farnesoid X receptor
  JOURNAL   Phytomedicine 42, 34-42 (2018)
   PUBMED   29655695
REFERENCE   5  (bases 1 to 3544)
  AUTHORS   Han,H., Xu,L., Xiong,K., Zhang,T. and Wang,Z.
  TITLE     Exploration of Hepatoprotective Effect of Gentiopicroside on
            Alpha-Naphthylisothiocyanate-Induced Cholestatic Liver Injury in
            Rats by Comprehensive Proteomic and Metabolomic Signatures
  JOURNAL   Cell Physiol Biochem 49 (4), 1304-1319 (2018)
   PUBMED   30223280
REFERENCE   6  (bases 1 to 3544)
  AUTHORS   Chiang,J.Y., Yang,T.P. and Wang,D.P.
  TITLE     Cloning and 5'-flanking sequence of a rat cholesterol 7
            alpha-hydroxylase gene
  JOURNAL   Biochim Biophys Acta 1132 (3), 337-339 (1992)
   PUBMED   1420318
REFERENCE   7  (bases 1 to 3544)
  AUTHORS   Nishimoto,M., Gotoh,O., Okuda,K. and Noshiro,M.
  TITLE     Structural analysis of the gene encoding rat cholesterol
            alpha-hydroxylase, the key enzyme for bile acid biosynthesis
  JOURNAL   J Biol Chem 266 (10), 6467-6471 (1991)
   PUBMED   2007596
REFERENCE   8  (bases 1 to 3544)
  AUTHORS   Jelinek,D.F. and Russell,D.W.
  TITLE     Structure of the rat gene encoding cholesterol 7 alpha-hydroxylase
  JOURNAL   Biochemistry 29 (34), 7781-7785 (1990)
   PUBMED   2261433
REFERENCE   9  (bases 1 to 3544)
  AUTHORS   Li,Y.C., Wang,D.P. and Chiang,J.Y.
  TITLE     Regulation of cholesterol 7 alpha-hydroxylase in the liver.
            Cloning, sequencing, and regulation of cholesterol 7
            alpha-hydroxylase mRNA
  JOURNAL   J Biol Chem 265 (20), 12012-12019 (1990)
   PUBMED   1694852
REFERENCE   10 (bases 1 to 3544)
  AUTHORS   Noshiro,M., Nishimoto,M. and Okuda,K.
  TITLE     Rat liver cholesterol 7 alpha-hydroxylase. Pretranslational
            regulation for circadian rhythm
  JOURNAL   J Biol Chem 265 (17), 10036-10041 (1990)
   PUBMED   1693613
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from J05460.1.
            
            On Aug 30, 2012 this sequence version replaced NM_012942.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J05460.1, J05430.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3544              J05460.1           2-3545
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="WKA"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q12"
     gene            1..3544
                     /gene="Cyp7a1"
                     /gene_synonym="CHAP; CYP7; CYP7S1"
                     /note="cytochrome P450 family 7 subfamily A member 1"
                     /db_xref="GeneID:25428"
                     /db_xref="RGD:2482"
     exon            1..114
                     /gene="Cyp7a1"
                     /gene_synonym="CHAP; CYP7; CYP7S1"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1546
                     /gene="Cyp7a1"
                     /gene_synonym="CHAP; CYP7; CYP7S1"
                     /EC_number="1.14.14.23"
                     /EC_number="1.14.14.26"
                     /note="cholesterol 7-alpha-monooxygenase; CYPVII;
                     cholesterol 7-alpha-hydroxylase; cytochrome P450
                     (cholesterol hydroxylase 7 alpha); cytochrome P450, 7a1;
                     cytochrome P450, family 7, subfamily a, polypeptide 1;
                     24-hydroxycholesterol 7-alpha-hydroxylase"
                     /codon_start=1
                     /product="cytochrome P450 7A1"
                     /protein_id="NP_037074.1"
                     /db_xref="GeneID:25428"
                     /db_xref="RGD:2482"
                     /translation="MMTISLIWGIAVLVSCCIWFIVGIRRRKAGEPPLENGLIPYLGC
                     ALKFGSNPLEFLRANQRKHGHVFTCKLMGKYVHFITNSLSYHKVLCHGKYFDWKKFHY
                     TTSAKAFGHRSIDPNDGNTTENINNTFTKTLQGDALCSLSEAMMQNLQSVMRPPGLPK
                     SKSNAWVTEGMYAFCYRVMFEAGYLTLFGRDISKTDTQKALILNNLDNFKQFDQVFPA
                     LVAGLPIHLFKTAHKAREKLAEGLKHKNLCVRDQVSELIRLRMFLNDTLSTFDDMEKA
                     KTHLAILWASQANTIPATFWSLFQMIRSPEAMKAASEEVSGALQSAGQELSSGGSAIY
                     LDQVQLNDLPVLDSIIKEALRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDMIALYPQ
                     LMHLDPEIYPDPLTFKYDRYLDESGKAKTTFYSNGNKLKCFYMPFGSGATICPGRLFA
                     VQEIKQFLILMLSCFELEFVESQVKCPPLDQSRAGLGILPPLHDIEFKYKLKH"
     exon            115..355
                     /gene="Cyp7a1"
                     /gene_synonym="CHAP; CYP7; CYP7S1"
                     /inference="alignment:Splign:2.1.0"
     exon            356..942
                     /gene="Cyp7a1"
                     /gene_synonym="CHAP; CYP7; CYP7S1"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1073
                     /gene="Cyp7a1"
                     /gene_synonym="CHAP; CYP7; CYP7S1"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1249
                     /gene="Cyp7a1"
                     /gene_synonym="CHAP; CYP7; CYP7S1"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..3544
                     /gene="Cyp7a1"
                     /gene_synonym="CHAP; CYP7; CYP7S1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtctcccctt tggaaatttt cctgcttttg caaaatgatg actatttctt tgatttgggg
       61 aattgccgtg ttggtgagct gttgcatatg gtttattgtt ggaataagga gaaggaaagc
      121 tggtgaacct cctttggaga acgggttgat tccgtacctg ggctgtgctc tgaaatttgg
      181 atctaatcct cttgagttcc taagagctaa tcaaaggaag catggtcacg tttttacctg
      241 caaactgatg gggaaatatg tccatttcat cacaaactcc ctgtcatacc acaaagtctt
      301 atgtcatgga aaatattttg actggaaaaa atttcattac actacttctg cgaaggcatt
      361 tggacacaga agcattgacc caaatgatgg aaataccacg gaaaatataa acaacacttt
      421 taccaaaacc ctccagggag atgctctgtg ttcactttct gaagccatga tgcaaaacct
      481 ccaatctgtc atgagacctc ctggccttcc taaatcaaag agcaatgcct gggtcacgga
      541 agggatgtat gccttctgtt accgagtgat gtttgaagct ggctatctaa cactgtttgg
      601 cagagatatt tcaaagacag acacacaaaa agcacttatt ctaaacaacc ttgacaactt
      661 caaacaattt gaccaagtct ttccggcact ggtggcaggc cttcctattc acttgttcaa
      721 gaccgcacat aaagctcggg aaaagctggc tgagggattg aagcacaaga acctgtgtgt
      781 gagggaccag gtctctgaac tgatccgtct acgtatgttt ctcaatgaca cgctctccac
      841 ctttgacgac atggagaagg ccaagacgca cctcgctatt ctctgggcat ctcaagcaaa
      901 caccattcct gcaacctttt ggagcttatt tcaaatgatc aggagtcctg aagcaatgaa
      961 agcagcctct gaagaagtga gtggagcttt acagagtgct ggccaagagc tcagctctgg
     1021 agggagtgcc atttacttgg atcaagtgca actgaatgac ctgccggtac tagacagcat
     1081 catcaaggag gctctgaggc tttccagtgc atccttgaat atccgcacag ctaaggagga
     1141 cttcactctc catcttgagg acggttccta taacatccga aaagatgaca tgatagctct
     1201 ttatccacag ttaatgcact tggatcctga aatctaccca gaccctttga ctttcaaata
     1261 tgaccggtac cttgatgaaa gcgggaaagc aaagaccacc ttctacagta atggaaacaa
     1321 gctgaagtgt ttctacatgc ccttcggatc aggcgcgaca atatgtcctg gaagactctt
     1381 tgccgtccaa gaaatcaagc agtttttgat cctgatgctc tcctgctttg aactggagtt
     1441 tgtggagagc caagtcaagt gtccccctct agaccagtcc cgggcaggct tgggaatttt
     1501 gccaccacta catgatattg agtttaaata taaactgaaa cactgatacg tggttggaag
     1561 aagcgaacac tggatgatgt cacttggcgg ctgagagtca tcactaaaca ggccttcggg
     1621 accaatgctc actgatgcgc cctagcgact ggattagtgg gaagaacttt gttctcgctg
     1681 cccacattcc tgggtgttca catagctggg gccagagctt catcactttc agaaagcaat
     1741 gtcttttgta tttattttca aaatgaagat attccaattg gcaggatatt tttcctaagg
     1801 aaattgcttt atatttttat gaaaactacc aattaattat gaaagggctt gaaattcacg
     1861 ttttagtgaa attactgatt tttcactagt aaggttcttc aggtgtgaaa ctgtattata
     1921 aaaatgttgt aatgggtcac actgtgcttt gcataaaggt aaaggaaact atgtttcagc
     1981 cttttctgtg tctatgagct tcgaaaataa tcttactgtt ctagaaacac tggggaggtt
     2041 tcgacatgct ctcgctatat tttattttac tgttgctaga aattttcatt ccagttttca
     2101 actaccttat ctttccccca ttttgacatg catgccaatg agaagagtat tttttaggaa
     2161 ttaacaaggc acctcccaga accctaccct gagactttta agcctttaat cccagcactc
     2221 gagaagtaga gccaggcaga tctctgagtc tgaggttatt ctggtctaca tcagctccag
     2281 acaagccagg actacagaat gggatcttgt ctaaaaaata cagctaatct ttatgtcata
     2341 actgattatg aatcaaccta aaagataaat tttcaatcag gactcagaga aaatgagcaa
     2401 ttaaaaaact tagctctgag gtatgtggaa ttcattaagt acaagttgac attacatgtt
     2461 ctttaaaaat agtttatgtt ttatctctaa atgccctgca gatgaagaat aataatgaaa
     2521 agttgaataa tactgtttaa acactaagtg caataatgct ttggtaatgt actttaagag
     2581 aatcattagc cgtgccagtt ttactaaaat atatttatat gtaaattata tttatctttt
     2641 tcttatacca taaatataaa aatattgcaa catttagtaa ttttaaaatt atataccttt
     2701 cagaaaatga tgtatgaatg tttgtatgtt ttttaacttt gaacagaaca tttaaattat
     2761 tcatctacgg tgatttttat cttatttatt tctttttgtc tcattcatat cttgaagaaa
     2821 tccaaaaata tctgaaggaa tcgctcactc aaatgtctcc ctatggttac agaaaaattc
     2881 aataccatgt ttttgtcctc ggggactgaa gcagggtgtc gtgggtgccg agcagaggct
     2941 cctgctgcag cgagctttat ccacgggact ccttaaactt ttaaaatctt atcactatta
     3001 tcatgcattt attacctaag taggatattt ccctttcctt tttcatttca gccgagtccc
     3061 ttagcaaccc aggctgactg ggaccctcca tgtagcttaa gctgtgaact cactgtactt
     3121 cctgttttca cttattttag gaagtaattt tccctatcag aaattttaat tgtttagatg
     3181 atgtataaga gtaacacaat tctgttatat actaatctgt agtaaactaa atttgttctt
     3241 agaacaagtt tgatgactct caaattgaat gtatccatac atctttccat ggcttcttga
     3301 atgcccattt ctcatacaca gaatgatggg tttcacggtg atgtcttcct ttcatgtctt
     3361 tattcttgtg cggtgatggt tggcaaatga tacccatgga gcaaggttac tcttcctatt
     3421 tctgtgcagc ctaagtgtta agaataattt ttaaatactt ggagggaagg cacattttgt
     3481 gtcatatgtg aagtgacatg tgacacacag actagcaaat ccttgagtaa aattttattg
     3541 ggat
//
