LOCUS       NM_001399701            2155 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus cytochrome P450, family 46, subfamily a,
            polypeptide 1 (Cyp46a1), mRNA.
ACCESSION   NM_001399701 XM_006240548
VERSION     NM_001399701.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Goyal,S., Xiao,Y., Porter,N.A., Xu,L. and Guengerich,F.P.
  TITLE     Oxidation of 7-dehydrocholesterol and desmosterol by human
            cytochrome P450 46A1
  JOURNAL   J Lipid Res 55 (9), 1933-1943 (2014)
   PUBMED   25017465
  REMARK    Erratum:[J Lipid Res. 2015 Nov;56(11):2226. PMID: 26523000]
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Mast,N., Zheng,W., Stout,C.D. and Pikuleva,I.A.
  TITLE     Binding of a cyano- and fluoro-containing drug bicalutamide to
            cytochrome P450 46A1: unusual features and spectral response
  JOURNAL   J Biol Chem 288 (7), 4613-4624 (2013)
   PUBMED   23288837
REFERENCE   3  (bases 1 to 2155)
  AUTHORS   Mast,N., Charvet,C., Pikuleva,I.A. and Stout,C.D.
  TITLE     Structural basis of drug binding to CYP46A1, an enzyme that
            controls cholesterol turnover in the brain
  JOURNAL   J Biol Chem 285 (41), 31783-31795 (2010)
   PUBMED   20667828
REFERENCE   4  (bases 1 to 2155)
  AUTHORS   Smiljanic,K., Lavrnja,I., Mladenovic Djordjevic,A., Ruzdijic,S.,
            Stojiljkovic,M., Pekovic,S. and Kanazir,S.
  TITLE     Brain injury induces cholesterol 24-hydroxylase (Cyp46) expression
            in glial cells in a time-dependent manner
  JOURNAL   Histochem Cell Biol 134 (2), 159-169 (2010)
   PUBMED   20559650
  REMARK    GeneRIF: in pathological conditions, like brain injury, Cyp46
            displayed atypical expression, being expressed not only in neuronal
            cells, but also in microglia and astrocytes
REFERENCE   5  (bases 1 to 2155)
  AUTHORS   Tian,G., Kong,Q., Lai,L., Ray-Chaudhury,A. and Lin,C.L.
  TITLE     Increased expression of cholesterol 24S-hydroxylase results in
            disruption of glial glutamate transporter EAAT2 association with
            lipid rafts: a potential role in Alzheimer's disease
  JOURNAL   J Neurochem 113 (4), 978-989 (2010)
   PUBMED   20193040
REFERENCE   6  (bases 1 to 2155)
  AUTHORS   Kotti,T.J., Ramirez,D.M., Pfeiffer,B.E., Huber,K.M. and
            Russell,D.W.
  TITLE     Brain cholesterol turnover required for geranylgeraniol production
            and learning in mice
  JOURNAL   Proc Natl Acad Sci U S A 103 (10), 3869-3874 (2006)
   PUBMED   16505352
REFERENCE   7  (bases 1 to 2155)
  AUTHORS   Mast,N., Norcross,R., Andersson,U., Shou,M., Nakayama,K.,
            Bjorkhem,I. and Pikuleva,I.A.
  TITLE     Broad substrate specificity of human cytochrome P450 46A1 which
            initiates cholesterol degradation in the brain
  JOURNAL   Biochemistry 42 (48), 14284-14292 (2003)
   PUBMED   14640697
REFERENCE   8  (bases 1 to 2155)
  AUTHORS   Lund,E.G., Xie,C., Kotti,T., Turley,S.D., Dietschy,J.M. and
            Russell,D.W.
  TITLE     Knockout of the cholesterol 24-hydroxylase gene in mice reveals a
            brain-specific mechanism of cholesterol turnover
  JOURNAL   J Biol Chem 278 (25), 22980-22988 (2003)
   PUBMED   12686551
REFERENCE   9  (bases 1 to 2155)
  AUTHORS   Kolsch,H., Lutjohann,D., Ludwig,M., Schulte,A., Ptok,U., Jessen,F.,
            von Bergmann,K., Rao,M.L., Maier,W. and Heun,R.
  TITLE     Polymorphism in the cholesterol 24S-hydroxylase gene is associated
            with Alzheimer's disease
  JOURNAL   Mol Psychiatry 7 (8), 899-902 (2002)
   PUBMED   12232784
REFERENCE   10 (bases 1 to 2155)
  AUTHORS   Lund,E.G., Guileyardo,J.M. and Russell,D.W.
  TITLE     cDNA cloning of cholesterol 24-hydroxylase, a mediator of
            cholesterol homeostasis in the brain
  JOURNAL   Proc Natl Acad Sci U S A 96 (13), 7238-7243 (1999)
   PUBMED   10377398
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Jan 13, 2022 this sequence version replaced XM_006240548.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC162019.1, SRR26643293.37254.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-221               JAXUCZ010000006.1  133011990-133012210
            222-302             JAXUCZ010000006.1  133019731-133019811
            303-384             JAXUCZ010000006.1  133020482-133020563
            385-458             JAXUCZ010000006.1  133023774-133023847
            459-545             JAXUCZ010000006.1  133024284-133024370
            546-684             JAXUCZ010000006.1  133028881-133029019
            685-795             JAXUCZ010000006.1  133029527-133029637
            796-946             JAXUCZ010000006.1  133030434-133030584
            947-1009            JAXUCZ010000006.1  133030739-133030801
            1010-1082           JAXUCZ010000006.1  133032779-133032851
            1083-1167           JAXUCZ010000006.1  133034843-133034927
            1168-1278           JAXUCZ010000006.1  133035449-133035559
            1279-1367           JAXUCZ010000006.1  133037444-133037532
            1368-1434           JAXUCZ010000006.1  133038138-133038204
            1435-2155           JAXUCZ010000006.1  133038447-133039167
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q32"
     gene            1..2155
                     /gene="Cyp46a1"
                     /note="cytochrome P450, family 46, subfamily a,
                     polypeptide 1"
                     /db_xref="GeneID:362782"
                     /db_xref="RGD:1306605"
     exon            1..221
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..1605
                     /gene="Cyp46a1"
                     /EC_number="1.14.14.25"
                     /codon_start=1
                     /product="cholesterol 24-hydroxylase"
                     /protein_id="NP_001386630.1"
                     /db_xref="GeneID:362782"
                     /db_xref="RGD:1306605"
                     /translation="MSPGLLLLGSAVLLAFGLCCTFVHRARSRYEHIPGPPRPSFLLG
                     HLPYFWKKDEACGRVLQDVFLDWAKKYGPVVRVNVFHKTSVIVTSPESVKKFLMSTKY
                     NKDSKMYRAIQTVFGERLFGQGLVSECDYGRWYKQRRVMDLAFSRSSLVSLMGTFNEK
                     AEQLMEILEAKADGQTPVSMQDMLTCATIDILAKAAFGMETSMLLGAQKPLSQAVKVM
                     LEGISASRNTLAKFMPGKRKQLREIRESIRLLRQVGKDWVQRRREALKRGEDVPADIL
                     TQILKAEEGAQDDEVLLDNFVTFFIAGHETSANHLAFTVMELSRQPEIVARLQAEVDE
                     VVGSKRHLDYEDLGRLQYLSQVLKESLRLYPPAWGTFRLLEEETLIDGVRVPGNTPLL
                     FSTYVMGRMDTYFEDPLTFNPDRFGPGAPKPRFTYFPFSLGHRSCIGQQFAQMEVKVV
                     MAKLLQRLEFRLVPGQRFGLQEQATLKPLDPVLCTLRPRGWQPAPPPPPC"
     exon            222..302
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            303..384
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            385..458
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            459..545
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            546..684
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            685..795
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            796..946
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1009
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1082
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1167
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1278
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1367
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1434
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..2155
                     /gene="Cyp46a1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcggggcct ggggccgagg cggcgcgcgg cgctgacagc tagtcggtcg cggcctccgg
       61 cccccttggc acctggcgcc gaccccggca gggctcggag ccatgagccc cgggctgctg
      121 ttgctcggca gtgccgtcct gctcgccttc ggcctctgct gcaccttcgt gcatcgcgct
      181 cgcagccgct atgagcacat ccccgggccg ccgcggccca gcttccttct gggacatctc
      241 ccctactttt ggaaaaagga cgaagcttgt ggccgtgtgc tccaagacgt gtttttggat
      301 tgggctaaga agtatggtcc tgttgtgaga gtcaatgtct tccacaaaac ctcagtcatc
      361 gtcacgagtc ctgagtcagt caagaagttc ctgatgtcca ccaagtacaa caaggactcc
      421 aagatgtacc gtgcgattca gactgtgttc ggagagagac tgtttggcca gggcttggtg
      481 tctgaatgtg actatgggcg ctggtataag cagagaagag tcatggactt ggccttcagt
      541 cgcagctcct tggttagcct gatggggacg ttcaatgaga aggcagagca gctgatggaa
      601 attctagaag ctaaggcgga cggacagaca ccggtgtcca tgcaggacat gctgacctgt
      661 gccaccatcg acatcctggc caaggcagct tttgggatgg agaccagtat gctactgggc
      721 gcccagaaac ccctgtccca ggcagtgaag gtcatgctgg agggtatcag tgcgtcccgt
      781 aacaccctgg cgaagttcat gccagggaag agaaagcagc ttcgggagat ccgagagagc
      841 atccgcctgt tacgccaggt ggggaaggat tgggtgcagc gccgccgtga ggccctgaag
      901 aggggtgagg acgtgccggc cgacatcctc acgcagattc tcaaagctga agagggagcc
      961 caggacgacg aggttctgct cgacaacttt gttaccttct tcattgcggg tcatgagact
     1021 tccgccaacc atctggcatt cacagtgatg gagttgtctc gacagccgga gatcgtggca
     1081 aggctgcagg ccgaggtgga tgaggtggtc ggttccaagc ggcacctgga ctacgaggat
     1141 ctggggagac tgcagtacct gtcacaggtc ctcaaagagt ctctgaggct gtacccacca
     1201 gcatggggca cctttcgcct gctggaagag gagaccttga ttgatggggt cagagtccct
     1261 ggcaacactc ctctcctgtt cagcacctat gtcatgggga ggatggacac ctactttgag
     1321 gatccactga ctttcaaccc tgaccgcttc ggccctggag cgcccaagcc gaggtttacc
     1381 tacttcccct tttccctggg ccaccggtcc tgcatcgggc agcagtttgc tcagatggag
     1441 gtgaaagtcg ttatggccaa gctgcttcag aggctcgaat tccggctggt acctgggcag
     1501 cgctttgggc tgcaagagca ggctacgctc aagccactgg accctgtgct gtgcaccctg
     1561 aggccccggg gctggcagcc tgcaccccca cccccaccct gctgagccct gggtagggtc
     1621 agactcctcg gaccagggcc tcattggccc ccattgctgc ccatggccac ccactggcgc
     1681 ccctgaccct tctgccacct tccacgatgg ctcctggcag gccctgtccc ctcgcctgct
     1741 tcacaccctc gtgcttcctg tcatctgctg gcttttcagc tctttctggt gggccctgcc
     1801 tgacccccag ctaccaccgc caccatcctc atctctccca aacctctgaa cttctgcact
     1861 ggccacaccc tcaaaagaga ctccctaact cctgctcact ccctaatctc ttcaagtgac
     1921 acatccccca ggaagccctc cttgctaccc acggccaggc caaggtcttt cctctgtgct
     1981 cctacctcta acacactgac cacactgtat tgtgaatgtc tgctcatgtg accagctgcc
     2041 ctgccaggct gtgagcacca cagggcaggg tctgtgtgca ccttgcctct gaggcccctg
     2101 ctgcaaacta gggtttggaa cagagtcgat gcccaataaa tgtgtgttga ctgca
//
