LOCUS       NM_001198676            1885 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus cytochrome P450, family 2, subfamily b,
            polypeptide 2 (Cyp2b2), mRNA.
ACCESSION   NM_001198676 XM_001062335 XM_001070953
VERSION     NM_001198676.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Kobayashi,M., Hoshinaga,Y., Miura,N., Tokuda,Y., Shigeoka,S.,
            Murai,A. and Horio,F.
  TITLE     Ascorbic acid deficiency decreases hepatic cytochrome P-450,
            especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1
            gene expression in scurvy-prone ODS rats
  JOURNAL   Biosci Biotechnol Biochem 78 (6), 1060-1066 (2014)
   PUBMED   25036135
  REMARK    GeneRIF: stimulation of hepatic heme oxygenase-1 expression by
            ascorbic acid deficiency caused the decrease in CYP content in
            liver
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Sun,H., Lee,C.M., Tripathi,S., Kim,K.B. and Morgan,E.T.
  TITLE     Nitric oxide-dependent CYP2B degradation is potentiated by a
            cytokine-regulated pathway and utilizes the immunoproteasome
            subunit LMP2
  JOURNAL   Biochem J 445 (3), 377-382 (2012)
   PUBMED   22612225
  REMARK    GeneRIF: LMP2 contributes to the NO-dependent degradation of CYP2B
            proteins, and induction of LMP2 may be involved in the potentiation
            of this degradation by IL-1.
REFERENCE   3  (bases 1 to 1885)
  AUTHORS   Saito,K., Kobayashi,K., Mizuno,Y., Fukuchi,Y., Furihata,T. and
            Chiba,K.
  TITLE     Peroxisome proliferator-activated receptor alpha (PPARalpha)
            agonists induce constitutive androstane receptor (CAR) and
            cytochrome P450 2B in rat primary hepatocytes
  JOURNAL   Drug Metab Pharmacokinet 25 (1), 108-111 (2010)
   PUBMED   20208394
REFERENCE   4  (bases 1 to 1885)
  AUTHORS   Rastogi,S., Khanna,S.K. and Das,M.
  TITLE     A spectrophotometric method to monitor the catalytic activity of
            microsomal cytochrome P-450 IIB1/2: comparison with fluorometric
            assay
  JOURNAL   Indian J Biochem Biophys 39 (3), 191-196 (2002)
   PUBMED   22905390
  REMARK    GeneRIF: analysis of the spectrophotometric method to monitor the
            catalytic activity of microsomal cytochrome P-450 IIB1/2 and
            comparison with fluorometric assay
REFERENCE   5  (bases 1 to 1885)
  AUTHORS   Hoffmann,M., Mager,W.H., Scholte,B.J., Civil,A. and Planta,R.J.
  TITLE     Analysis of the promoter of the cytochrome P-450 2B2 gene in the
            rat
  JOURNAL   Gene Expr 2 (4), 353-363 (1992)
   PUBMED   1335316
REFERENCE   6  (bases 1 to 1885)
  AUTHORS   Lacroix,D., Desrochers,M., Lambert,M. and Anderson,A.
  TITLE     Alternative splicing of mRNA encoding rat liver cytochrome P450e
            (P450IIB2)
  JOURNAL   Gene 86 (2), 201-207 (1990)
   PUBMED   2323573
REFERENCE   7  (bases 1 to 1885)
  AUTHORS   Aoyama,T., Korzekwa,K., Nagata,K., Adesnik,M., Reiss,A.,
            Lapenson,D.P., Gillette,J., Gelboin,H.V., Waxman,D.J. and
            Gonzalez,F.J.
  TITLE     Sequence requirements for cytochrome P-450IIB1 catalytic activity.
            Alteration of the stereospecificity and regioselectivity of steroid
            hydroxylation by a simultaneous change of two hydrophobic amino
            acid residues to phenylalanine
  JOURNAL   J Biol Chem 264 (35), 21327-21333 (1989)
   PUBMED   2574176
REFERENCE   8  (bases 1 to 1885)
  AUTHORS   Pyerin,W. and Taniguchi,H.
  TITLE     Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450
  JOURNAL   EMBO J 8 (10), 3003-3010 (1989)
   PUBMED   2583091
REFERENCE   9  (bases 1 to 1885)
  AUTHORS   Oesch,F., Waxman,D.J., Morrissey,J.J., Honscha,W., Kissel,W. and
            Friedberg,T.
  TITLE     Antibodies targeted against hypervariable and constant regions of
            cytochromes P450IIB1 and P450IIB2
  JOURNAL   Arch Biochem Biophys 270 (1), 23-32 (1989)
   PUBMED   2539047
REFERENCE   10 (bases 1 to 1885)
  AUTHORS   Hashimoto,T., Matsumoto,T., Nishizawa,M., Kawabata,S.,
            Morohashi,K., Handa,S. and Omura,T.
  TITLE     A mutant rat strain deficient in induction of a
            phenobarbital-inducible form of cytochrome P-450 in liver
            microsomes
  JOURNAL   J Biochem 103 (3), 487-492 (1988)
   PUBMED   2839467
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1 and M34452.1.
            
            On or before Nov 4, 2010 this sequence version replaced
            XM_001070953.2, XM_001062335.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript and protein alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643286.946.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               JAXUCZ010000001.1  90722281-90722451
            172-311             JAXUCZ010000001.1  90725534-90725673
            312-1885            M34452.1           1-1574
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..1885
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /note="cytochrome P450, family 2, subfamily b, polypeptide
                     2"
                     /db_xref="GeneID:361523"
                     /db_xref="RGD:2467"
     CDS             1..1500
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /EC_number="1.14.14.1"
                     /note="cytochrome P450 2B2; cytochrome P450, subfamily IIB
                     (phenobarbital-inducible)(b, e); cytochrome P-450e-M;
                     cytochrome P-450b type e; cytochrome P450E; cytochrome
                     P450 PB4"
                     /codon_start=1
                     /product="cytochrome P450 2B2 precursor"
                     /protein_id="NP_001185605.1"
                     /db_xref="GeneID:361523"
                     /db_xref="RGD:2467"
                     /translation="MEPSILLLLALLVGFLLLLVRGHPKSRGNFPPGPRPLPLLGNLL
                     QLDRGGLLNSFMQFREKYGDVFTVHLGPRPVVMLCGTDTIKEALVGQAEDFSGRGTIA
                     VIEPIFKEYGVFFANGERWKALRRFSLATMRDFGMGKRSVEERIQEEAQCLVEELRKS
                     QGAPLDPTFLFQCITANIICSIVFGERFDYTDRQFLRLLELFYRTFSLLSSFSSQVFE
                     FFSGFLKYFPGAHRQISKNLQEILDYIGHIVEKHRATLDPSAPRDFIDTYLLRMEKVS
                     PAWMREEKSNHHTEFHHENLMISLLSLFFAGTETGSTTLRYGFLLMLKYPHVTEKVQK
                     EIDQVIGSHRPPSLDDRTKMPYTDAVIHEIQRFADLAPIGLPHRVTKDTMFRGYLLPK
                     NTEVYPILSSALHDPQYFDHPDTFNPEHFLDADGTLKKSEAFMPFSTGKRICLGEGIA
                     RNELFLFFTTILQNFSVSSHLAPKDIDLTPKESGIAKIPPTYQICFSAR"
     exon            1..171
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
     exon            172..334
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
     exon            335..484
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
     exon            485..645
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
     exon            646..846
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
     exon            847..988
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1176
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1318
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1885
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atggagccca gtatcttgct cctccttgct ctccttgtgg gcttcttgtt actcttagtc
       61 aggggacacc caaagtcccg tggcaacttc ccaccaggac ctcgtcccct tcccctcttg
      121 gggaacctcc tgcagttgga cagaggaggc ctcctcaatt ccttcatgca gtttcgcgaa
      181 aaatatggag atgtgttcac agtacacctg ggaccaaggc ctgtggtcat gctatgtggg
      241 acagacacca taaaggaggc tctggtgggc caagctgaag atttctctgg tcggggaaca
      301 atcgctgtga ttgagccaat cttcaaggaa tatggtgtgt tctttgccaa tggggaacgc
      361 tggaaggccc ttcggcgatt ctctctggct accatgagag actttgggat gggaaagagg
      421 agtgtggaag aacggattca ggaggaagcc caatgtttgg tggaggaact gcggaaatcc
      481 cagggagccc cactggatcc caccttcctc ttccagtgca tcacagccaa catcatctgc
      541 tccattgtgt ttggagagcg ctttgactac acagaccgcc agttcctgcg cctgttggag
      601 ctgttctacc ggaccttttc cctcctaagt tcattctcca gccaggtgtt tgagttcttc
      661 tctgggttcc tgaaatactt tcctggtgcc cacagacaaa tctccaaaaa cctccaggaa
      721 atcctcgatt acattggcca tattgtggag aagcacaggg ccaccttaga ccccagcgct
      781 ccacgagact tcatcgacac ttaccttctg cgcatggaga aagtgagtcc tgcatggatg
      841 agagaggaga agtcgaacca ccacacagag ttccatcatg agaacctcat gatctccctg
      901 ctctctctct tctttgctgg cactgagacc ggcagcacca cactccgcta tggtttcctg
      961 ctcatgctca agtaccccca tgtcacagag aaagtccaaa aggagattga tcaggtgatt
     1021 ggctctcaca ggccaccatc ccttgatgat cgtaccaaaa tgccatacac tgatgcagtc
     1081 atccacgaga ttcagagatt tgcagatctt gccccaattg gtttaccaca cagagtcacc
     1141 aaagacacca tgttccgagg gtacctgctc cccaagaaca ctgaggtgta tcccatcctg
     1201 agttcagctc tccatgaccc acagtacttt gaccatccag acaccttcaa tcctgagcac
     1261 ttcctggatg ccgatgggac actgaaaaag agtgaagctt ttatgccctt ctccacagga
     1321 aagcgcattt gtcttggcga aggcattgcc cgaaatgaat tgttcctctt cttcaccacc
     1381 atcctccaga acttctctgt gtcaagccat ttggctccca aggacattga cctcacgccc
     1441 aaggagagtg gcattgcaaa aatacctcca acataccaga tctgcttctc agctcggtga
     1501 tcgggctgag gcagccaggt gccccagttc tgttgggaat ggcctcatgt ttctgcctct
     1561 gggggacctg ctgaaaacca ggctcaaggc cactgctcac atcttcctat tgcagttctc
     1621 caaagtccca aggcttgttc ttattcctgt gaatggcact gaagaagtca atcgactgtc
     1681 ttattttgac atgtgaacag agatttcatg agtacacatc tcatgctgag tcacttccct
     1741 cttcctccta atagcccacg tccccactta tcagccctcc atggtctgtg atctgtgcta
     1801 atggactctg tatatggtct cagtgctatg tctacagact tacatagtat gtatggttca
     1861 ggtaaacaga atcacagagt gtgtg
//
