LOCUS       NP_001185605             499 aa            linear   ROD 30-MAR-2024
DEFINITION  cytochrome P450 2B2 precursor [Rattus norvegicus].
ACCESSION   NP_001185605 XP_001062335 XP_001070953
VERSION     NP_001185605.1
DBSOURCE    REFSEQ: accession NM_001198676.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 499)
  AUTHORS   Kobayashi,M., Hoshinaga,Y., Miura,N., Tokuda,Y., Shigeoka,S.,
            Murai,A. and Horio,F.
  TITLE     Ascorbic acid deficiency decreases hepatic cytochrome P-450,
            especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1
            gene expression in scurvy-prone ODS rats
  JOURNAL   Biosci Biotechnol Biochem 78 (6), 1060-1066 (2014)
   PUBMED   25036135
  REMARK    GeneRIF: stimulation of hepatic heme oxygenase-1 expression by
            ascorbic acid deficiency caused the decrease in CYP content in
            liver
REFERENCE   2  (residues 1 to 499)
  AUTHORS   Sun,H., Lee,C.M., Tripathi,S., Kim,K.B. and Morgan,E.T.
  TITLE     Nitric oxide-dependent CYP2B degradation is potentiated by a
            cytokine-regulated pathway and utilizes the immunoproteasome
            subunit LMP2
  JOURNAL   Biochem J 445 (3), 377-382 (2012)
   PUBMED   22612225
  REMARK    GeneRIF: LMP2 contributes to the NO-dependent degradation of CYP2B
            proteins, and induction of LMP2 may be involved in the potentiation
            of this degradation by IL-1.
REFERENCE   3  (residues 1 to 499)
  AUTHORS   Saito,K., Kobayashi,K., Mizuno,Y., Fukuchi,Y., Furihata,T. and
            Chiba,K.
  TITLE     Peroxisome proliferator-activated receptor alpha (PPARalpha)
            agonists induce constitutive androstane receptor (CAR) and
            cytochrome P450 2B in rat primary hepatocytes
  JOURNAL   Drug Metab Pharmacokinet 25 (1), 108-111 (2010)
   PUBMED   20208394
REFERENCE   4  (residues 1 to 499)
  AUTHORS   Rastogi,S., Khanna,S.K. and Das,M.
  TITLE     A spectrophotometric method to monitor the catalytic activity of
            microsomal cytochrome P-450 IIB1/2: comparison with fluorometric
            assay
  JOURNAL   Indian J Biochem Biophys 39 (3), 191-196 (2002)
   PUBMED   22905390
  REMARK    GeneRIF: analysis of the spectrophotometric method to monitor the
            catalytic activity of microsomal cytochrome P-450 IIB1/2 and
            comparison with fluorometric assay
REFERENCE   5  (residues 1 to 499)
  AUTHORS   Hoffmann,M., Mager,W.H., Scholte,B.J., Civil,A. and Planta,R.J.
  TITLE     Analysis of the promoter of the cytochrome P-450 2B2 gene in the
            rat
  JOURNAL   Gene Expr 2 (4), 353-363 (1992)
   PUBMED   1335316
REFERENCE   6  (residues 1 to 499)
  AUTHORS   Lacroix,D., Desrochers,M., Lambert,M. and Anderson,A.
  TITLE     Alternative splicing of mRNA encoding rat liver cytochrome P450e
            (P450IIB2)
  JOURNAL   Gene 86 (2), 201-207 (1990)
   PUBMED   2323573
REFERENCE   7  (residues 1 to 499)
  AUTHORS   Aoyama,T., Korzekwa,K., Nagata,K., Adesnik,M., Reiss,A.,
            Lapenson,D.P., Gillette,J., Gelboin,H.V., Waxman,D.J. and
            Gonzalez,F.J.
  TITLE     Sequence requirements for cytochrome P-450IIB1 catalytic activity.
            Alteration of the stereospecificity and regioselectivity of steroid
            hydroxylation by a simultaneous change of two hydrophobic amino
            acid residues to phenylalanine
  JOURNAL   J Biol Chem 264 (35), 21327-21333 (1989)
   PUBMED   2574176
REFERENCE   8  (residues 1 to 499)
  AUTHORS   Pyerin,W. and Taniguchi,H.
  TITLE     Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450
  JOURNAL   EMBO J 8 (10), 3003-3010 (1989)
   PUBMED   2583091
REFERENCE   9  (residues 1 to 499)
  AUTHORS   Oesch,F., Waxman,D.J., Morrissey,J.J., Honscha,W., Kissel,W. and
            Friedberg,T.
  TITLE     Antibodies targeted against hypervariable and constant regions of
            cytochromes P450IIB1 and P450IIB2
  JOURNAL   Arch Biochem Biophys 270 (1), 23-32 (1989)
   PUBMED   2539047
REFERENCE   10 (residues 1 to 499)
  AUTHORS   Hashimoto,T., Matsumoto,T., Nishizawa,M., Kawabata,S.,
            Morohashi,K., Handa,S. and Omura,T.
  TITLE     A mutant rat strain deficient in induction of a
            phenobarbital-inducible form of cytochrome P-450 in liver
            microsomes
  JOURNAL   J Biochem 103 (3), 487-492 (1988)
   PUBMED   2839467
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1 and M34452.1.
            
            On or before Nov 4, 2010 this sequence version replaced
            XP_001070953.2, XP_001062335.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript and protein alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643286.946.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     Protein         1..499
                     /product="cytochrome P450 2B2 precursor"
                     /EC_number="1.14.14.1"
                     /note="cytochrome P450 2B2; cytochrome P450, subfamily IIB
                     (phenobarbital-inducible)(b, e); cytochrome P-450e-M;
                     cytochrome P-450b type e; cytochrome P450E; cytochrome
                     P450 PB4"
                     /calculated_mol_wt=54582
     sig_peptide     1..22
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2394
     Region          62..494
                     /region_name="CYP2B"
                     /note="cytochrome P450 family 2, subfamily B; cd20672"
                     /db_xref="CDD:410765"
     Site            order(73,98,101,104,114..115,206,209,302,305..306,
                     309..310,370..371,373..376,397,444,484..486)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410765"
     Site            order(98,113..114,121,125,179,306..307,310..311,314,365,
                     371,375,377,400,436..438,442,444..446,449..450)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410765"
     CDS             1..499
                     /gene="Cyp2b2"
                     /gene_synonym="Cype; CYPIIB2"
                     /coded_by="NM_001198676.1:1..1500"
                     /db_xref="GeneID:361523"
                     /db_xref="RGD:2467"
ORIGIN      
        1 mepsilllla llvgfllllv rghpksrgnf ppgprplpll gnllqldrgg llnsfmqfre
       61 kygdvftvhl gprpvvmlcg tdtikealvg qaedfsgrgt iaviepifke ygvffanger
      121 wkalrrfsla tmrdfgmgkr sveeriqeea qclveelrks qgapldptfl fqcitaniic
      181 sivfgerfdy tdrqflrlle lfyrtfslls sfssqvfeff sgflkyfpga hrqisknlqe
      241 ildyighive khratldpsa prdfidtyll rmekvspawm reeksnhhte fhhenlmisl
      301 lslffagtet gsttlrygfl lmlkyphvte kvqkeidqvi gshrppsldd rtkmpytdav
      361 iheiqrfadl apiglphrvt kdtmfrgyll pkntevypil ssalhdpqyf dhpdtfnpeh
      421 fldadgtlkk seafmpfstg kriclgegia rnelflfftt ilqnfsvssh lapkdidltp
      481 kesgiakipp tyqicfsar
//
