LOCUS       NM_012692               1687 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus cytochrome P450, family 2, subfamily a,
            polypeptide 1 (Cyp2a1), mRNA.
ACCESSION   NM_012692
VERSION     NM_012692.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Lewis,D.F. and Lake,B.G.
  TITLE     Species differences in coumarin metabolism: a molecular modelling
            evaluation of CYP2A interactions
  JOURNAL   Xenobiotica 32 (7), 547-561 (2002)
   PUBMED   12162851
  REMARK    GeneRIF: the rat orthologue (CYP2A1) exhibits 3,4-epoxidation of
            coumarin
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Hanioka,N., Gonzalez,F.J., Lindberg,N.A., Liu,G. and Korzekwa,K.R.
  TITLE     Chimeric cDNA expression and site directed mutagenesis studies of
            cytochrome P450s CYP2A1 and CYP2A2
  JOURNAL   J Steroid Biochem Mol Biol 43 (8), 1037-1043 (1992)
   PUBMED   22217848
  REMARK    GeneRIF: Data suggest that structures of active sites of CYP2A1 and
            CYP2A2 have certain amount of flexibility and that non-specific
            binding (i.e., hydrophobic binding) is involved in differences in
            substrate specificity between these two enzymes.
REFERENCE   3  (bases 1 to 1687)
  AUTHORS   Matsunaga,T., Nomoto,M., Kozak,C.A. and Gonzalez,F.J.
  TITLE     Structure and in vitro transcription of the rat CYP2A1 and CYP2A2
            genes and regional localization of the CYP2A gene subfamily on
            mouse chromosome 7
  JOURNAL   Biochemistry 29 (5), 1329-1341 (1990)
   PUBMED   2322568
REFERENCE   4  (bases 1 to 1687)
  AUTHORS   Arlotto,M.P., Greenway,D.J. and Parkinson,A.
  TITLE     Purification of two isozymes of rat liver microsomal cytochrome
            P450 with testosterone 7 alpha-hydroxylase activity
  JOURNAL   Arch Biochem Biophys 270 (2), 441-457 (1989)
   PUBMED   2650624
REFERENCE   5  (bases 1 to 1687)
  AUTHORS   Imaoka,S., Kamataki,T. and Funae,Y.
  TITLE     Purification and characterization of six cytochromes P-450 from
            hepatic microsomes of immature female rats
  JOURNAL   J Biochem 102 (4), 843-851 (1987)
   PUBMED   3436956
REFERENCE   6  (bases 1 to 1687)
  AUTHORS   Nagata,K., Matsunaga,T., Gillette,J., Gelboin,H.V. and
            Gonzalez,F.J.
  TITLE     Rat testosterone 7 alpha-hydroxylase. Isolation, sequence, and
            expression of cDNA and its developmental regulation and induction
            by 3-methylcholanthrene
  JOURNAL   J Biol Chem 262 (6), 2787-2793 (1987)
   PUBMED   3818621
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from J02669.1.
            
            [WARNING] On Oct 7, 2024 this sequence was replaced by NM_012692.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J02669.1, BC081848.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..1687
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /note="cytochrome P450, family 2, subfamily a, polypeptide
                     1"
                     /db_xref="GeneID:24894"
                     /db_xref="RGD:2463"
     exon            1..196
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..1498
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /EC_number="1.14.14.1"
                     /note="CYPIIA1; P450-UT-F; testosterone
                     7-alpha-hydroxylase; steroid hormones 7-alpha-hydroxylase;
                     cytochrome P450-UT-F; cytochrome P450 IIA1 (hepatic
                     steroid hydroxylase IIA1); steroid hydroxylase, hepatic"
                     /codon_start=1
                     /product="cytochrome P450 2A1"
                     /protein_id="NP_036824.1"
                     /db_xref="GeneID:24894"
                     /db_xref="RGD:2463"
                     /translation="MLDTGLLLVVILASLSVMLLVSLWQQKIRGRLPPGPTPLPFIGN
                     YLQLNTKDVYSSITQLSERYGPVFTIHLGPRRVVVLYGYDAVKEALVDQAEEFSGRGE
                     QATYNTLFKGYGVAFSSGERAKQLRRLSIATLRDFGVGKRGVEERILEEAGYLIKMLQ
                     GTCGAPIDPTIYLSKTVSNVISSIVFGERFDYEDTEFLSLLQMMGQMNRFAASPTGQL
                     YDMFHSVMKYLPGPQQQIIKVTQKLEDFMIEKVRQNHSTLDPNSPRNFIDSFLIRMQE
                     EKNGNSEFHMKNLVMTTLSLFFAGSETVSSTLRYGFLLLMKHPDVEAKVHEEIEQVIG
                     RNRQPQYEDHMKMPYTQAVINEIQRFSNLAPLGIPRRIIKNTTFRGFFLPKATDVFPI
                     LGSLMTDPKFFPSPKDFDPQNFLDDKGQLKKNAAFLPFSTGKRFCLGDGLAKMELFLL
                     LTTILQNFRFKFPMKLEDINESPKPLGFTRIIPKYTMSFMPI"
     exon            197..359
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
     exon            360..509
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
     exon            510..670
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
     exon            671..847
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
     exon            848..986
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1174
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1316
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1687
                     /gene="Cyp2a1"
                     /gene_synonym="RATCYP2A1; Shdl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcaatcctca ctggccacta tgctggacac aggactgctt ctggtggtca tattggcctc
       61 cctgagcgtc atgctcttgg tgtccctctg gcagcagaaa atcaggggga gattgcctcc
      121 aggacccact cctttgcctt tcattggaaa ttatctgcag ctgaatacaa aagacgtata
      181 cagttccatc acacagctca gtgagcgcta tggtcctgtg ttcaccatcc accttgggcc
      241 tcgccgggtt gtggtgcttt atggatacga tgcagtcaaa gaggctttgg tggaccaagc
      301 tgaggagttc agtggacgag gcgaacaggc tacctacaat acactcttca aaggctatgg
      361 cgtggcattc agcagtgggg agcgggcaaa acaactcagg cgcctctcta tagccacatt
      421 gagagatttt ggtgtgggca agcgtggtgt agaggagcgt atcctggagg aggcaggcta
      481 tttgatcaag atgttgcagg gcacttgtgg agcccccatt gaccccacca tctacctgag
      541 caaaacagtc tccaatgtta ttagctccat tgtcttcggg gaacgcttcg actatgagga
      601 cacggagttc ctgtcactgc tgcagatgat gggtcaaatg aacagatttg cagcttcacc
      661 cacagggcag ctctatgaca tgttccattc agtgatgaag tacctgcctg gaccacagca
      721 acagatcatc aaggttactc agaaactgga agacttcatg atagagaaag tgaggcagaa
      781 ccatagtacc ctggacccca attccccaag gaacttcatt gactcctttc tcatccgcat
      841 gcaagaggag aaaaatggca attcagagtt ccacatgaag aacctagtga tgacaacact
      901 aagcctcttc tttgctgggt ctgagacagt cagctccaca ctacgctacg gcttccttct
      961 actcatgaag catccagatg tggaggccaa ggtccatgag gaaattgagc aggtgatcgg
     1021 caggaaccga cagcctcagt atgaggacca catgaagatg ccctacaccc aggctgtgat
     1081 caatgagatc caaagatttt ctaacttggc tcccttgggc attcctcgaa ggattatcaa
     1141 gaacacaacc ttccgtggct tcttcctccc caaggccacc gatgtgttcc ctatattagg
     1201 ttctctgatg acagacccaa agttcttccc tagccccaaa gacttcgacc cccagaactt
     1261 cctggatgac aagggacagt tgaagaaaaa tgctgctttc ctccctttct ccactgggaa
     1321 gcgattctgc ttgggagatg gcctggctaa gatggagctc ttcctgctgc tcaccactat
     1381 tttacagaac ttccgtttca agttcccaat gaaactagaa gacatcaacg agtcccccaa
     1441 acccttgggg tttaccagga tcataccaaa gtacaccatg agcttcatgc ccatctgatt
     1501 ctgagttgaa tcaaggtggg gcaagaggga gagagagcct gaagtggggc cagggtgcag
     1561 gtggagagaa caggggaggt gaagatgagg gttaagaagg gaccacaccc atggaagaaa
     1621 cacaaaagac ttctcacttt ggtaaaattg taacagtcct aataaaaaga aagaaatact
     1681 cagtcgg
//
