LOCUS       NM_178847               2150 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Rattus norvegicus cytochrome P450, family 27, subfamily a,
            polypeptide 1 (Cyp27a1), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_178847 XM_217439
VERSION     NM_178847.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Jiao,N., Baker,S.S., Chapa-Rodriguez,A., Liu,W., Nugent,C.A.,
            Tsompana,M., Mastrandrea,L., Buck,M.J., Baker,R.D., Genco,R.J.,
            Zhu,R. and Zhu,L.
  TITLE     Suppressed hepatic bile acid signalling despite elevated production
            of primary and secondary bile acids in NAFLD
  JOURNAL   Gut 67 (10), 1881-1891 (2018)
   PUBMED   28774887
REFERENCE   3  (bases 1 to 2150)
  AUTHORS   Zhu,L., Wang,L., Cao,F., Liu,P., Bao,H., Yan,Y., Dong,X., Wang,D.,
            Wang,Z. and Gong,P.
  TITLE     Modulation of transport and metabolism of bile acids and bilirubin
            by chlorogenic acid against hepatotoxicity and cholestasis in bile
            duct ligation rats: involvement of SIRT1-mediated deacetylation of
            FXR and PGC-1alpha
  JOURNAL   J Hepatobiliary Pancreat Sci 25 (3), 195-205 (2018)
   PUBMED   29360226
REFERENCE   4  (bases 1 to 2150)
  AUTHORS   Li,J., Daly,E., Campioli,E., Wabitsch,M. and Papadopoulos,V.
  TITLE     De novo synthesis of steroids and oxysterols in adipocytes
  JOURNAL   J Biol Chem 289 (2), 747-764 (2014)
   PUBMED   24280213
  REMARK    GeneRIF: Data indicate that inhibition of CYP27A1 activity or
            knockdown and deletion of the Cyp27a1 gene induced adipocyte
            differentiation.
REFERENCE   5  (bases 1 to 2150)
  AUTHORS   Gilardi,F., Viviani,B., Galmozzi,A., Boraso,M., Bartesaghi,S.,
            Torri,A., Caruso,D., Crestani,M., Marinovich,M. and de Fabiani,E.
  TITLE     Expression of sterol 27-hydroxylase in glial cells and its
            regulation by liver X receptor signaling
  JOURNAL   Neuroscience 164 (2), 530-540 (2009)
   PUBMED   19665519
  REMARK    GeneRIF: Cyp27a1 is present in glial cells and is regulated in
            response to LXR activation, consistent with the role played by
            astrocytes in the control of cholesterol metabolism and homeostasis
            in the brain in physiological conditions
REFERENCE   6  (bases 1 to 2150)
  AUTHORS   Kitanaka,S., Takeyama,K., Murayama,A., Sato,T., Okumura,K.,
            Nogami,M., Hasegawa,Y., Niimi,H., Yanagisawa,J., Tanaka,T. and
            Kato,S.
  TITLE     Inactivating mutations in the 25-hydroxyvitamin D3
            1alpha-hydroxylase gene in patients with pseudovitamin D-deficiency
            rickets
  JOURNAL   N Engl J Med 338 (10), 653-661 (1998)
   PUBMED   9486994
REFERENCE   7  (bases 1 to 2150)
  AUTHORS   Mullick,J., Addya,S., Sucharov,C. and Avadhani,N.G.
  TITLE     Localization of a transcription promoter within the second exon of
            the cytochrome P-450c27/25 gene for the expression of the major
            species of two-kilobase mRNA
  JOURNAL   Biochemistry 34 (42), 13729-13742 (1995)
   PUBMED   7577965
REFERENCE   8  (bases 1 to 2150)
  AUTHORS   Cali,J.J., Hsieh,C.L., Francke,U. and Russell,D.W.
  TITLE     Mutations in the bile acid biosynthetic enzyme sterol
            27-hydroxylase underlie cerebrotendinous xanthomatosis
  JOURNAL   J Biol Chem 266 (12), 7779-7783 (1991)
   PUBMED   2019602
REFERENCE   9  (bases 1 to 2150)
  AUTHORS   Su,P., Rennert,H., Shayiq,R.M., Yamamoto,R., Zheng,Y.M., Addya,S.,
            Strauss,J.F. 3rd and Avadhani,N.G.
  TITLE     A cDNA encoding a rat mitochondrial cytochrome P450 catalyzing both
            the 26-hydroxylation of cholesterol and 25-hydroxylation of vitamin
            D3: gonadotropic regulation of the cognate mRNA in ovaries
  JOURNAL   DNA Cell Biol 9 (9), 657-667 (1990)
   PUBMED   2175615
REFERENCE   10 (bases 1 to 2150)
  AUTHORS   Usui,E., Noshiro,M. and Okuda,K.
  TITLE     Molecular cloning of cDNA for vitamin D3 25-hydroxylase from rat
            liver mitochondria
  JOURNAL   FEBS Lett 262 (1), 135-138 (1990)
   PUBMED   2318307
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB734352.1 and BC061848.1.
            
            On Oct 3, 2014 this sequence version replaced NM_178847.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061848.1, M38566.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG   :: upstream AUG and CDS
                                                  extension is not conserved
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-246               CB734352.1         1-246
            247-2150            BC061848.1         1-1904
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q33"
     gene            1..2150
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /note="cytochrome P450, family 27, subfamily a,
                     polypeptide 1"
                     /db_xref="GeneID:301517"
                     /db_xref="RGD:727915"
     exon            1..546
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
     CDS             286..1887
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /EC_number="1.14.15.15"
                     /note="cytochrome P-450c27/25; cytochrome P450 27; sterol
                     27-hydroxylase;
                     5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol
                     27-hydroxylase; vitamin D(3) 25-hydroxylase;
                     5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol
                     26-hydroxylase"
                     /codon_start=1
                     /product="sterol 26-hydroxylase, mitochondrial"
                     /protein_id="NP_849178.2"
                     /db_xref="GeneID:301517"
                     /db_xref="RGD:727915"
                     /translation="MAVLSRMRLRWALLDTRVMGHGLCPQGARAKAAIPAALRDHEST
                     EGPGTGQDRPRLRSLAELPGPGTLRFLFQLFLRGYVLHLHELQALNKAKYGPMWTTTF
                     GTRTNVNLASAPLLEQVMRQEGKYPIRDSMEQWKEHRDHKGLSYGIFITQGQQWYHLR
                     HSLNQRMLKPAEAALYTDALNEVISDFIARLDQVRTESASGDQVPDVAHLLYHLALEA
                     ICYILFEKRVGCLEPSIPEDTATFIRSVGLMFKNSVYVTFLPKWSRPLLPFWKRYMNN
                     WDNIFSFGEKMIHQKVQEIEAQLQAAGPDGVQVSGYLHFLLTKELLSPQETVGTFPEL
                     ILAGVDTTSNTLTWALYHLSKNPEIQEALHKEVTGVVPFGKVPQNKDFAHMPLLKAVI
                     KETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPESFQPH
                     RWLRKREDDNSGIQHPFGSVPFGYGVRSCLGRRIAELEMQLLLSRLIQKYEVVLSPGM
                     GEVKSVSRIVLVPSKKVSLRFLQRQ"
     exon            547..737
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
     exon            738..937
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1135
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1308
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1475
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1557
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1770
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..2129
                     /gene="Cyp27a1"
                     /gene_synonym="Cyp27; P450C27"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctgctacc gccagagagc tccgaacttt tccctaggcc accttgtcca cctctgcctg
       61 ccttcttagt aggggtcttg cagctggaga aggttgcatt tgtgtgttcg acacatcctg
      121 attggaaggg gttatgaaag gatctcgctt gtcctctatc ggcccgcccc gggatcacct
      181 ctgcctttaa agtcgccagg tcgcgcaaag ctggagttct gcctccttgc ctggatgggg
      241 cgcgtagtct ctggctctaa actcttggct tctcagacac gatctatggc tgtgttgagc
      301 cgcatgagac tgagatgggc gcttctggac actcgtgtga tgggccatgg cctctgccca
      361 caaggggcca gagccaaggc cgcgatccct gcagccctcc gggatcacga gagcacggag
      421 ggtccaggaa caggtcaaga ccgaccgcgc ctgcggagtc tggcggagct tccgggaccc
      481 ggaacgctac gctttttatt ccagctattt ctacgaggct atgtgctgca cttgcacgag
      541 ctccaggcgc tgaacaaggc caagtacggc ccaatgtgga caaccacctt tgggactcgc
      601 accaatgtga atctggctag cgccccgctc ttggagcaag tgatgagaca ggagggcaag
      661 taccccataa gagacagcat ggagcagtgg aaggagcacc gagaccacaa aggcctctcc
      721 tatgggatct tcatcacaca aggacagcag tggtaccatc tgcgtcatag tttgaatcag
      781 cggatgctga agcctgctga ggcagccctc tacacagatg ccttaaacga ggtcatcagt
      841 gactttattg cccggctgga ccaggtgcgg acagagagtg catcagggga tcaggtgcca
      901 gatgtggcac atcttctcta ccaccttgcc ttggaagcca tctgctatat cctgtttgag
      961 aaaagggttg gctgcctgga gccctccatc cctgaggaca ccgccacctt catcagatct
     1021 gttggactca tgttcaagaa ctcagtctat gtcactttcc ttcccaagtg gtctcggcct
     1081 ctgctgccct tttggaagcg atacatgaat aactgggata acattttctc cttcggggag
     1141 aagatgattc atcaaaaagt ccaggagata gaagcccagc tacaggcggc tgggccagat
     1201 ggggtccagg tatctggcta cctgcacttc ctgctgacta aggaattgct cagtcctcaa
     1261 gagactgtcg gcacctttcc tgagctgatc ttggctgggg tagacacgac atccaataca
     1321 ctgacctggg ccctgtatca cctttcaaag aacccagaga tccaggaagc cttgcacaag
     1381 gaagtgactg gtgtggtacc cttcgggaag gtgccccaga acaaggactt tgcccacatg
     1441 cccctgctaa aagctgtgat taaggagacc ctgcgcctct accctgtggt tcccacaaac
     1501 tcccggatca tcacagaaaa ggaaactgaa attaatggct tcctcttccc taagaataca
     1561 cagtttgtgt tatgccacta cgtggtgtcc cgagatccca gtgtctttcc tgagcccgag
     1621 agcttccagc ctcaccgatg gctgaggaag agagaggacg ataactccgg gatccaacac
     1681 ccatttggct ctgtgccctt tggctatggg gttcggtcct gcctgggtcg caggattgca
     1741 gaactggaga tgcaactcct gctgtcaagg ctgatacaaa agtatgaggt ggtcctgtct
     1801 cccgggatgg gagaagtgaa gtctgtgtcc cgcatcgtcc tggttcccag caagaaggtg
     1861 agcctacgct ttctgcagag acagtagtac caagctgggc tcctgtctcc atgggacttg
     1921 tccagaagcc ctggcacaga agttcttggc cagtctcacg tcacatgtca cgatgccaga
     1981 ttcaacaggg gacctctctg cccttcccat agacaccaga cgtctggcac aatctctact
     2041 gagcagcacc catttaagac attagagcac ctcatatcac aggacggtgc ttgggtacaa
     2101 tttaaaataa aatttaaaat tcaaatttga aaaaaaaaaa aaaaaaaaaa
//
