LOCUS       NM_057101               1963 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus cytochrome P450, family 21, subfamily a,
            polypeptide 1 (Cyp21a1), mRNA.
ACCESSION   NM_057101 XM_001076389
VERSION     NM_057101.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Nishizato,Y., Imai,S., Yabuki,M., Kido,H. and Komuro,S.
  TITLE     Development of relevant assay system to identify steroidogenic
            enzyme inhibitors
  JOURNAL   Toxicol In Vitro 24 (2), 677-685 (2010)
   PUBMED   19665544
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Huang,X.J., Ihsan,A., Wang,X., Dai,M.H., Wang,Y.L., Su,S.J.,
            Xue,X.J. and Yuan,Z.H.
  TITLE     Long-term dose-dependent response of Mequindox on aldosterone,
            corticosterone and five steroidogenic enzyme mRNAs in the adrenal
            of male rats
  JOURNAL   Toxicol Lett 191 (2-3), 167-173 (2009)
   PUBMED   19733641
REFERENCE   3  (bases 1 to 1963)
  AUTHORS   Payne,A.H. and Hales,D.B.
  TITLE     Overview of steroidogenic enzymes in the pathway from cholesterol
            to active steroid hormones
  JOURNAL   Endocr Rev 25 (6), 947-970 (2004)
   PUBMED   15583024
  REMARK    Review article
REFERENCE   4  (bases 1 to 1963)
  AUTHORS   Hurt,P., Walter,L., Sudbrak,R., Klages,S., Muller,I., Shiina,T.,
            Inoko,H., Lehrach,H., Gunther,E., Reinhardt,R. and Himmelbauer,H.
  TITLE     The genomic sequence and comparative analysis of the rat major
            histocompatibility complex
  JOURNAL   Genome Res 14 (4), 631-639 (2004)
   PUBMED   15060004
REFERENCE   5  (bases 1 to 1963)
  AUTHORS   Lovelace,M., Watson,T.G. and Stephenson,G.L.
  TITLE     Steroid 21-hydroxylase expression in cultured rat astrocytes
  JOURNAL   Brain Res Bull 61 (6), 609-615 (2003)
   PUBMED   14519458
  REMARK    GeneRIF: Expression of steroid 21-hydroxylase has been detected in
            cultured rat cerebellar astrocytes.
REFERENCE   6  (bases 1 to 1963)
  AUTHORS   Speiser,P.W. and White,P.C.
  TITLE     Congenital adrenal hyperplasia
  JOURNAL   N Engl J Med 349 (8), 776-788 (2003)
   PUBMED   12930931
  REMARK    Review article
REFERENCE   7  (bases 1 to 1963)
  AUTHORS   Walter,L., Hurt,P., Himmelbauer,H., Sudbrak,R. and Gunther,E.
  TITLE     Physical mapping of the major histocompatibility complex class II
            and class III regions of the rat
  JOURNAL   Immunogenetics 54 (4), 268-275 (2002)
   PUBMED   12136338
REFERENCE   8  (bases 1 to 1963)
  AUTHORS   Zhou,M.Y., del Carmen Vila,M., Gomez-Sanchez,E.P. and
            Gomez-Sanchez,C.E.
  TITLE     Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat
            adrenal
  JOURNAL   J Steroid Biochem Mol Biol 62 (4), 277-286 (1997)
   PUBMED   9408081
REFERENCE   9  (bases 1 to 1963)
  AUTHORS   Brake,P.B. and Jefcoate,C.R.
  TITLE     Regulation of cytochrome P4501B1 in cultured rat adrenocortical
            cells by cyclic adenosine 3',5'-monophosphate and 2,3,7,8-
            tetrachlorodibenzo-p-dioxin
  JOURNAL   Endocrinology 136 (11), 5034-5041 (1995)
   PUBMED   7588239
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX883044.1.
            
            On Sep 28, 2009 this sequence version replaced NM_057101.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U56853.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132291,
                                           SAMD00132308 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               BX883044.1         337163-337378       c
            217-306             BX883044.1         337009-337098       c
            307-449             BX883044.1         336492-336634       c
            450-545             BX883044.1         336294-336389       c
            546-650             BX883044.1         336085-336189       c
            651-737             BX883044.1         335898-335984       c
            738-938             BX883044.1         335573-335773       c
            939-1108            BX883044.1         335235-335404       c
            1109-1212           BX883044.1         335061-335164       c
            1213-1963           BX883044.1         334226-334976       c
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..1963
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /note="cytochrome P450, family 21, subfamily a,
                     polypeptide 1"
                     /db_xref="GeneID:24298"
                     /db_xref="RGD:2461"
     exon            1..216
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     CDS             9..1490
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /EC_number="1.14.14.16"
                     /note="cytochrome P450, subfamily XXI (steroid
                     21-hydroxylase); 21-OHase; P-450c21; P450-C21; cytochrome
                     P450 XXI; cytochrome P-450c21; cytochrome P450-C21;
                     Cytochrome P450 subfamily XXI (steroid 21-hydroxylase);
                     cytochrome P450, subfamily 21A, polypeptide 1; cytochrome
                     P450 21 steroid 21 hydroxylase"
                     /codon_start=1
                     /product="steroid 21-hydroxylase"
                     /protein_id="NP_476442.2"
                     /db_xref="GeneID:24298"
                     /db_xref="RGD:2461"
                     /translation="MLLPGLLLLLLLLLLAGTRWLWGQWKLWKLRLPPLAPGFLHFLQ
                     PNLPVYLFGLAQKLGPIYRIRLGLQDVVVLNSNKTIEEALIQKWVDFAGRPQILDGKM
                     NFDLSMGDYSLTWKAHKKLSRSALVLGMRDSMEPLVEQLTQEFCERMRAQAGASVAIH
                     KEFSLLTCSIISCLTFGDKQDSTLLNATHSCVRDLLKAWNHWSVQILDIIPFLRFFPN
                     PGLWKLKQFQESRDHIVMQELKRHKDSLVAGQWKDMIDYMLQGVEKQRDARDPGQLHE
                     RHVHMSVVDLFVGGTETTAATLSWAVAFLLHHPEIQKRLQEELDLKLAPSSQLLYKNR
                     MQLPLLMATIAEVLRLRPVVPMALPHRATKASSISGYDIPKDTIIIPNIQGANLDEMV
                     WELPSKFWPDRFLESGKSPRIPTFGCGARVCLGEPLARLELFVVLARLLQTFTLLPPP
                     DGTLPSLQPLPYTGINLLIPPFQVRLQPRNLAPQDQGQKSSTG"
     exon            217..306
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     exon            307..449
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     exon            450..545
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     exon            546..650
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     exon            651..737
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     exon            738..938
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1108
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1212
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1963
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttgggccat gctgctacct gggctgctgt tgctgctgct gctgctgctg ctagctggca
       61 cccgctggct gtggggtcag tggaagctct ggaaactccg cctcccgcct ctggccccgg
      121 ggtttctgca ctttctacag cccaaccttc cggtctacct gtttggcctg gctcagaaac
      181 ttgggcccat ctacaggatc cgcttggggc tacaagatgt ggtggtgttg aactctaaca
      241 aaaccattga ggaggccttg atccaaaagt gggtggactt tgctggccga ccccagatac
      301 tagatggaaa gatgaacttt gacctctcca tgggggatta ttctctaaca tggaaagccc
      361 acaagaaact ctctcgttcg gccctggtgc tgggcatgag agactccatg gaacctctgg
      421 tagagcagct gacccaggaa ttctgtgagc gcatgagagc ccaggccggt gcctctgtgg
      481 ccatccataa ggaattctcc ttactcacct gcagtatcat cagctgtctc actttcggag
      541 acaagcagga cagcactttg ctgaatgcca ctcacagctg tgtccgggac ttgctgaaag
      601 cctggaatca ctggtccgtc caaatcttgg atataattcc ctttctcagg ttcttcccca
      661 atccaggcct ctggaaactg aagcagttcc aggagagtag ggaccatatc gtaatgcagg
      721 agctgaagag gcacaaggac agcctggtgg cagggcagtg gaaagacatg attgactaca
      781 tgctccaggg tgtggagaag caaagagatg ccagagaccc agggcagctc cacgagcggc
      841 acgtgcatat gtcggtggtg gacctttttg tcggtggcac ggagaccacg gctgccacgc
      901 tctcctgggc tgtggctttc ctgcttcacc accctgagat ccagaagcgg ctgcaggaag
      961 aattagacct caagctggcc cccagctccc agctcctgta caagaaccga atgcagctac
     1021 ctctgctcat ggccaccatt gctgaagtgc tgcgtttgcg gcccgtggtg cccatggcct
     1081 tgccccatcg tgcaactaag gctagcagca tctctggcta tgatatcccc aaggatacga
     1141 tcatcatccc caacatccaa ggcgccaacc tggatgagat ggtttgggaa ctgcccagca
     1201 agttctggcc tgatcgcttc ctggaatccg gcaagagtcc cagaataccg acctttggct
     1261 gtggggcacg cgtgtgcctg ggcgagcctc tggcgcggct ggagctcttc gtggtgctgg
     1321 ctcgcctgct ccagaccttc acgctgctgc cgccaccaga tggtaccctg ccctccctgc
     1381 agcccctgcc gtatactggc atcaatctct taattcctcc cttccaggtg cggctgcagc
     1441 ccaggaacct ggcaccccaa gaccagggcc agaaatcctc gacaggatag gacgagcctt
     1501 gtgcctcagt ttctccttta ttgctccatt cccctcccct cccccctgta aacatggtgc
     1561 tgtgagactg tggcagagaa ggcttcctcg gtgagctagg cacagtagag acacactggc
     1621 tcttctctgg gcacaacccc tcagtgctcg gcagtcatac cagggtgtga gtgaggtggg
     1681 cagcaggctc aggtgcccct ggtgggggcc tggaagtttc tgggtctcag cttcatttcc
     1741 gtgaagggca ccgagaactc gaagcccttc cagtggtacc agctcactcc ctgagggaag
     1801 ggttgtcaag ggaggagagt caagacaacg ccacatctat gccaccttat ccatcccaac
     1861 cctggctcct ctaggtgact cccagagtgc agccatctcc ccccatgatc cctgaagtga
     1921 tgtcctctcc cacccgctaa taaacggtct tttcaagatt ccc
//
