LOCUS       NP_476442                493 aa            linear   ROD 03-APR-2024
DEFINITION  steroid 21-hydroxylase [Rattus norvegicus].
ACCESSION   NP_476442 XP_001076389
VERSION     NP_476442.2
DBSOURCE    REFSEQ: accession NM_057101.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 493)
  AUTHORS   Nishizato,Y., Imai,S., Yabuki,M., Kido,H. and Komuro,S.
  TITLE     Development of relevant assay system to identify steroidogenic
            enzyme inhibitors
  JOURNAL   Toxicol In Vitro 24 (2), 677-685 (2010)
   PUBMED   19665544
REFERENCE   2  (residues 1 to 493)
  AUTHORS   Huang,X.J., Ihsan,A., Wang,X., Dai,M.H., Wang,Y.L., Su,S.J.,
            Xue,X.J. and Yuan,Z.H.
  TITLE     Long-term dose-dependent response of Mequindox on aldosterone,
            corticosterone and five steroidogenic enzyme mRNAs in the adrenal
            of male rats
  JOURNAL   Toxicol Lett 191 (2-3), 167-173 (2009)
   PUBMED   19733641
REFERENCE   3  (residues 1 to 493)
  AUTHORS   Payne,A.H. and Hales,D.B.
  TITLE     Overview of steroidogenic enzymes in the pathway from cholesterol
            to active steroid hormones
  JOURNAL   Endocr Rev 25 (6), 947-970 (2004)
   PUBMED   15583024
  REMARK    Review article
REFERENCE   4  (residues 1 to 493)
  AUTHORS   Hurt,P., Walter,L., Sudbrak,R., Klages,S., Muller,I., Shiina,T.,
            Inoko,H., Lehrach,H., Gunther,E., Reinhardt,R. and Himmelbauer,H.
  TITLE     The genomic sequence and comparative analysis of the rat major
            histocompatibility complex
  JOURNAL   Genome Res 14 (4), 631-639 (2004)
   PUBMED   15060004
REFERENCE   5  (residues 1 to 493)
  AUTHORS   Lovelace,M., Watson,T.G. and Stephenson,G.L.
  TITLE     Steroid 21-hydroxylase expression in cultured rat astrocytes
  JOURNAL   Brain Res Bull 61 (6), 609-615 (2003)
   PUBMED   14519458
  REMARK    GeneRIF: Expression of steroid 21-hydroxylase has been detected in
            cultured rat cerebellar astrocytes.
REFERENCE   6  (residues 1 to 493)
  AUTHORS   Speiser,P.W. and White,P.C.
  TITLE     Congenital adrenal hyperplasia
  JOURNAL   N Engl J Med 349 (8), 776-788 (2003)
   PUBMED   12930931
  REMARK    Review article
REFERENCE   7  (residues 1 to 493)
  AUTHORS   Walter,L., Hurt,P., Himmelbauer,H., Sudbrak,R. and Gunther,E.
  TITLE     Physical mapping of the major histocompatibility complex class II
            and class III regions of the rat
  JOURNAL   Immunogenetics 54 (4), 268-275 (2002)
   PUBMED   12136338
REFERENCE   8  (residues 1 to 493)
  AUTHORS   Zhou,M.Y., del Carmen Vila,M., Gomez-Sanchez,E.P. and
            Gomez-Sanchez,C.E.
  TITLE     Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat
            adrenal
  JOURNAL   J Steroid Biochem Mol Biol 62 (4), 277-286 (1997)
   PUBMED   9408081
REFERENCE   9  (residues 1 to 493)
  AUTHORS   Brake,P.B. and Jefcoate,C.R.
  TITLE     Regulation of cytochrome P4501B1 in cultured rat adrenocortical
            cells by cyclic adenosine 3',5'-monophosphate and 2,3,7,8-
            tetrachlorodibenzo-p-dioxin
  JOURNAL   Endocrinology 136 (11), 5034-5041 (1995)
   PUBMED   7588239
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX883044.1.
            
            On Sep 28, 2009 this sequence version replaced NP_476442.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U56853.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132291,
                                           SAMD00132308 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     Protein         1..493
                     /product="steroid 21-hydroxylase"
                     /EC_number="1.14.14.16"
                     /note="cytochrome P450, subfamily XXI (steroid
                     21-hydroxylase); 21-OHase; P-450c21; P450-C21; cytochrome
                     P450 XXI; cytochrome P-450c21; cytochrome P450-C21;
                     Cytochrome P450 subfamily XXI (steroid 21-hydroxylase);
                     cytochrome P450, subfamily 21A, polypeptide 1; cytochrome
                     P450 21 steroid 21 hydroxylase"
                     /calculated_mol_wt=55781
     Region          58..477
                     /region_name="CYP21"
                     /note="cytochrome P450 21, also called steroid
                     21-hydroxylase; cd20674"
                     /db_xref="CDD:410767"
     Site            order(94,107,115,119,171,285..286,289..290,293..294,297,
                     348,353..354,357..358,360,383,415..416,421,423..425,
                     428..429)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410767"
     Site            order(105,107..108,195..196,199,228,231,284..285,288..289,
                     293,354,358,465)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410767"
     CDS             1..493
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; Cyp21"
                     /coded_by="NM_057101.2:9..1490"
                     /db_xref="GeneID:24298"
                     /db_xref="RGD:2461"
ORIGIN      
        1 mllpglllll lllllagtrw lwgqwklwkl rlpplapgfl hflqpnlpvy lfglaqklgp
       61 iyrirlglqd vvvlnsnkti eealiqkwvd fagrpqildg kmnfdlsmgd ysltwkahkk
      121 lsrsalvlgm rdsmeplveq ltqefcermr aqagasvaih kefslltcsi iscltfgdkq
      181 dstllnaths cvrdllkawn hwsvqildii pflrffpnpg lwklkqfqes rdhivmqelk
      241 rhkdslvagq wkdmidymlq gvekqrdard pgqlherhvh msvvdlfvgg tettaatlsw
      301 avafllhhpe iqkrlqeeld lklapssqll yknrmqlpll matiaevlrl rpvvpmalph
      361 ratkassisg ydipkdtiii pniqganlde mvwelpskfw pdrflesgks priptfgcga
      421 rvclgeplar lelfvvlarl lqtftllppp dgtlpslqpl pytginllip pfqvrlqprn
      481 lapqdqgqks stg
//
