LOCUS       NM_017286               1780 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Rattus norvegicus cytochrome P450, family 11, subfamily a,
            polypeptide 1 (Cyp11a1), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_017286
VERSION     NM_017286.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Fujiki,J., Maeda,N., Yamaguchi,K., Ohtsuki,Y. and Iwano,H.
  TITLE     DNA methylation of Ad4BP/SF-1 suppresses Cyp11a1 and StAR
            transcripts in C2C12 myoblasts
  JOURNAL   Mol Cell Endocrinol 593, 112336 (2024)
   PUBMED   39094930
  REMARK    GeneRIF: DNA methylation of Ad4BP/SF-1 suppresses Cyp11a1 and StAR
            transcripts in C2C12 myoblasts.
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Jeminiwa,B.O., Knight,R.C., Abbot,K.L., Pondugula,S.R. and
            Akingbemi,B.T.
  TITLE     Gonadal sex steroid hormone secretion after exposure of male rats
            to estrogenic chemicals and their combinations
  JOURNAL   Mol Cell Endocrinol 533, 111332 (2021)
   PUBMED   34038751
  REMARK    GeneRIF: Gonadal sex steroid hormone secretion after exposure of
            male rats to estrogenic chemicals and their combinations.
REFERENCE   3  (bases 1 to 1780)
  AUTHORS   Brzoskwinia,M., Pardyak,L., Kaminska,A., Tworzydlo,W., Hejmej,A.,
            Marek,S., Bilinski,S.M. and Bilinska,B.
  TITLE     Flutamide treatment reveals a relationship between steroidogenic
            activity of Leydig cells and ultrastructure of their mitochondria
  JOURNAL   Sci Rep 11 (1), 13772 (2021)
   PUBMED   34215832
  REMARK    GeneRIF: Flutamide treatment reveals a relationship between
            steroidogenic activity of Leydig cells and ultrastructure of their
            mitochondria.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1780)
  AUTHORS   Yu,C., Jiang,F., Zhang,M., Luo,D., Shao,S., Zhao,J., Gao,L., Zuo,C.
            and Guan,Q.
  TITLE     HC diet inhibited testosterone synthesis by activating endoplasmic
            reticulum stress in testicular Leydig cells
  JOURNAL   J Cell Mol Med 23 (5), 3140-3150 (2019)
   PUBMED   30884106
  REMARK    GeneRIF: these steroidogenic enzymes within the Leydig cells,
            including steroidogenic acute regulatory protein (StAR),
            cholesterol side-chain cleavage cytochrome P450 (P450scc) and
            3beta-hydroxysteroid dehydrogenase (3beta-HSD), were
            down-regulated.
REFERENCE   5  (bases 1 to 1780)
  AUTHORS   Lee,J.H., Lee,M., Ahn,C., Kang,H.Y., Tran,D.N. and Jeung,E.B.
  TITLE     Parabens Accelerate Ovarian Dysfunction in a 4-Vinylcyclohexene
            Diepoxide-Induced Ovarian Failure Model
  JOURNAL   Int J Environ Res Public Health 14 (2), 161 (2017)
   PUBMED   28208728
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1780)
  AUTHORS   O'Shaughnessy,P.J. and Mannan,M.A.
  TITLE     Development of cytochrome P-450 side chain cleavage mRNA levels in
            neonatal ovaries of normal and hypogonadal (hpg) mice
  JOURNAL   Mol Cell Endocrinol 104 (2), 133-138 (1994)
   PUBMED   7527351
REFERENCE   7  (bases 1 to 1780)
  AUTHORS   Oonk,R.B., Parker,K.L., Gibson,J.L. and Richards,J.S.
  TITLE     Rat cholesterol side-chain cleavage cytochrome P-450 (P-450scc)
            gene. Structure and regulation by cAMP in vitro
  JOURNAL   J Biol Chem 265 (36), 22392-22401 (1990)
   PUBMED   2176216
REFERENCE   8  (bases 1 to 1780)
  AUTHORS   Hanukoglu,I., Suh,B.S., Himmelhoch,S. and Amsterdam,A.
  TITLE     Induction and mitochondrial localization of cytochrome P450scc
            system enzymes in normal and transformed ovarian granulosa cells
  JOURNAL   J Cell Biol 111 (4), 1373-1381 (1990)
   PUBMED   2170421
REFERENCE   9  (bases 1 to 1780)
  AUTHORS   Oonk,R.B., Krasnow,J.S., Beattie,W.G. and Richards,J.S.
  TITLE     Cyclic AMP-dependent and -independent regulation of cholesterol
            side chain cleavage cytochrome P-450 (P-450scc) in rat ovarian
            granulosa cells and corpora lutea. cDNA and deduced amino acid
            sequence of rat P-450scc
  JOURNAL   J Biol Chem 264 (36), 21934-21942 (1989)
   PUBMED   2480959
REFERENCE   10 (bases 1 to 1780)
  AUTHORS   McMasters,K.M., Dickson,L.A., Shamy,R.V., Robischon,K.,
            Macdonald,G.J. and Moyle,W.R.
  TITLE     Rat cholesterol side-chain cleavage enzyme (P-450scc): use of a
            cDNA probe to study the hormonal regulation of P-450scc mRNA levels
            in ovarian granulosa cells
  JOURNAL   Gene 57 (1), 1-9 (1987)
   PUBMED   3123325
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Apr 13, 2016 this sequence version replaced NM_017286.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089100.1, J05156.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-288               JAXUCZ010000008.1  67318665-67318952
            289-444             JAXUCZ010000008.1  67320056-67320211
            445-644             JAXUCZ010000008.1  67322045-67322244
            645-848             JAXUCZ010000008.1  67323144-67323347
            849-1006            JAXUCZ010000008.1  67324709-67324866
            1007-1173           JAXUCZ010000008.1  67328264-67328430
            1174-1252           JAXUCZ010000008.1  67328702-67328780
            1253-1450           JAXUCZ010000008.1  67329246-67329443
            1451-1780           JAXUCZ010000008.1  67329867-67330196
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..1780
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /note="cytochrome P450, family 11, subfamily a,
                     polypeptide 1"
                     /db_xref="GeneID:29680"
                     /db_xref="RGD:69325"
     exon            1..288
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..1606
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /EC_number="1.14.15.6"
                     /note="cholesterol side-chain cleavage enzyme,
                     mitochondrial; cholesterol desmolase; cytochrome P450
                     side-chain cleavage enzyme; cytochrome P450 subfamily 11A;
                     cytochrome P450 11A1; cytochrome P450(scc); cytochrome
                     P450 11a cholesterol side chain cleavage; cholesterol
                     side-chain cleavage cytochrome P450; cytochrome P450
                     family 11 subfamily a"
                     /codon_start=1
                     /product="cholesterol side-chain cleavage enzyme,
                     mitochondrial precursor"
                     /protein_id="NP_058982.1"
                     /db_xref="GeneID:29680"
                     /db_xref="RGD:69325"
                     /translation="MLAKGLCLRSVLVKSCQPFLSPVWQGPGLATGNGAGISSTNSPR
                     SFNEIPSPGDNGWINLYHFLRENGTHRIHYHHMQNFQKYGPIYREKLGNMESVYILDP
                     KDAATLFSCEGPNPERYLVPPWVAYHQYYQRPIGVLFKSSDAWRKDRIVLNQEVMAPD
                     SIKNFVPLLEGVAQDFIKVLHRRIKQQNSGKFSGDISDDLFRFAFESITSVVFGERLG
                     MLEEIVDPESQRFIDAVYQMFHTSVPMLNMPPDLFRLFRTKTWKDHAAAWDVIFSKAD
                     EYTQNFYWDLRQKRDFSKYPGVLYSLLGGNKLPFKNIQANITEMLAGGVDTTSMTLQW
                     NLYEMAHNLKVQEMLRAEVLAARRQAQGDMAKMVQLVPLLKASIKETLRLHPISVTLQ
                     RYIVNDLVLRNYKIPAKTLVQVASYAMGRESSFFPNPNKFDPTRWLEKSQNTTHFRYL
                     GFGWGVRQCLGRRIAELEMTIFLINVLENFRIEVQSIRDVGTKFNLILMPEKPIFFNF
                     QPLKQDLGSTMPRKGDTV"
     exon            289..444
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
     exon            445..644
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
     exon            645..848
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
     exon            849..1006
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1173
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1252
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1450
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1780
                     /gene="Cyp11a1"
                     /gene_synonym="Cyp11a; Cypxia1; P450(scc); P450scc"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaaagtggc agtcgtgggg acagtatgct ggcaaaaggt ctttgcctgc gctcagtgct
       61 ggtcaaaagc tgccaacctt tcctgagccc tgtgtggcag ggtccagggc tggccactgg
      121 aaatggagct ggtatctcct ctaccaacag tcctcgatcc ttcaatgaga tcccttcccc
      181 tggtgacaat ggttggataa acctgtacca cttcctgagg gagaacggca cacacagaat
      241 ccattatcat cacatgcaga atttccagaa gtatggcccc atttacaggg agaagctggg
      301 caacatggag tcagtttaca tcctggaccc taaggacgca gcgacactct tctcctgcga
      361 gggtcctaac ccggagcgct accttgtgcc cccctgggtg gcctatcacc agtattacca
      421 gaggcccatt ggggtactgt ttaagagttc agatgcctgg aggaaagaca gaattgtcct
      481 aaaccaagag gtgatggccc ctgactccat caagaacttc gtgcccctgc tggaaggtgt
      541 agctcaggac ttcatcaaag tcttacacag acgcatcaag cagcaaaact ctggaaagtt
      601 ctcaggggac atcagcgatg acctattccg ctttgccttt gagtccatca ccagtgttgt
      661 atttggggag cgcctgggga tgttggagga gatcgtggac cctgagtccc agcggttcat
      721 cgacgccgtc taccagatgt tccacaccag tgttcccatg ctcaacatgc ctccagactt
      781 atttcgactc tttagaacta agacctggaa ggaccatgca gctgcctggg atgtgatttt
      841 cagtaaagct gatgaataca cccagaactt ctactgggac ttaaggcaga agcgagactt
      901 cagcaagtac cctggtgtcc tttatagcct cctgggaggc aacaagctgc ccttcaagaa
      961 catccaggcc aacatcacag agatgctggc aggaggggtg gacacgacct ccatgactct
     1021 gcaatggaac ctttatgaaa tggcacacaa cttgaaggta caggagatgc tgcgggctga
     1081 agttctagct gcccggcgcc aggcccaggg agacatggcc aagatggtcc agttggtccc
     1141 actcctcaaa gccagcatca aggagacact gagactccac cccatctctg tgaccttgca
     1201 gaggtatatt gtgaatgacc tggtgcttcg taattacaag attccagcca agactttggt
     1261 gcaggtggct agctatgcca tgggtcgaga atccagcttc tttcccaatc caaacaagtt
     1321 tgacccaact cgttggttgg aaaaaagcca aaacaccacg cacttccggt acttgggctt
     1381 tggctggggt gttcggcagt gtctgggccg gcggattgca gaactggaaa tgaccatctt
     1441 cctcatcaac gtgctggaaa acttcagaat tgaagttcaa agtatccgtg atgtggggac
     1501 caagttcaac ctcatcctga tgcctgagaa gcctatcttc ttcaacttcc agcctctcaa
     1561 gcaggacctg ggctcaacta tgcccagaaa aggcgacact gtatgaattg caggctgcag
     1621 tcacacgggg agatgtctca ctggggcatt tgagggtggt gtctctatat ctccagaaac
     1681 agcactctgt gattacctgt ccacgttagc tgggccctcc tctctttcat cctgtctccc
     1741 tttctactta ctcaggaggt taataaacac ataaatgctg
//
