LOCUS       NM_012840                681 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus cytochrome c, testis (Cyct), mRNA.
ACCESSION   NM_012840
VERSION     NM_012840.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Liu,Z., Lin,H., Ye,S., Liu,Q.Y., Meng,Z., Zhang,C.M., Xia,Y.,
            Margoliash,E., Rao,Z. and Liu,X.J.
  TITLE     Remarkably high activities of testicular cytochrome c in destroying
            reactive oxygen species and in triggering apoptosis
  JOURNAL   Proc Natl Acad Sci U S A 103 (24), 8965-8970 (2006)
   PUBMED   16757556
REFERENCE   2  (bases 1 to 681)
  AUTHORS   Matsuki,S., Iuchi,Y., Ikeda,Y., Sasagawa,I., Tomita,Y. and Fujii,J.
  TITLE     Suppression of cytochrome c release and apoptosis in testes with
            heat stress by minocycline
  JOURNAL   Biochem Biophys Res Commun 312 (3), 843-849 (2003)
   PUBMED   14680842
  REMARK    GeneRIF: These findings suggest that heat stress of testes triggers
            the release of cytochrome c from mitochondria in spermatogenic
            cells, leading to the activation of an apoptotic pathway.
REFERENCE   3  (bases 1 to 681)
  AUTHORS   Virbasius,J.V. and Scarpulla,R.C.
  TITLE     Structure and expression of rodent genes encoding the
            testis-specific cytochrome c. Differences in gene structure and
            evolution between somatic and testicular variants
  JOURNAL   J Biol Chem 263 (14), 6791-6796 (1988)
   PUBMED   2834389
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1 and M20623.1.
            
            On Aug 1, 2014 this sequence version replaced NM_012840.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643293.17265.1,
                                           SRR26360181.642152.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            regulatory uORF                    :: PMID: 2834389
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               JAXUCZ010000003.1  81704257-81704401   c
            146-174             JAXUCZ010000003.1  81701679-81701707   c
            175-680             M20623.1           5-510
            681-189428990       JAXUCZ010000003.1  1-189428310
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q24"
     gene            1..681
                     /gene="Cyct"
                     /gene_synonym="CYCTA"
                     /note="cytochrome c, testis"
                     /db_xref="GeneID:25310"
                     /db_xref="RGD:2452"
     exon            1..145
                     /gene="Cyct"
                     /gene_synonym="CYCTA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12..140
                     /regulatory_class="uORF"
                     /gene="Cyct"
                     /gene_synonym="CYCTA"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:2834389]"
                     /note="this uORF is predicted to encode a 42 aa peptide
                     (PMID:2834389)"
     exon            146..174
                     /gene="Cyct"
                     /gene_synonym="CYCTA"
                     /inference="alignment:Splign:2.1.0"
     exon            175..400
                     /gene="Cyct"
                     /gene_synonym="CYCTA"
                     /inference="alignment:Splign:2.1.0"
     CDS             232..549
                     /gene="Cyct"
                     /gene_synonym="CYCTA"
                     /note="Cytochrome C testis specific"
                     /codon_start=1
                     /product="cytochrome c, testis-specific"
                     /protein_id="NP_036972.1"
                     /db_xref="GeneID:25310"
                     /db_xref="RGD:2452"
                     /translation="MGDAEAGKKIFIQKCAQCHTVEKGGKHKTGPNLWGLFGRKTGQA
                     PGFSYTDANKNKGVIWTEETLMEYLENPKKYIPGTKMIFAGIKKKSEREDLIQYLKEA
                     TSS"
     exon            401..681
                     /gene="Cyct"
                     /gene_synonym="CYCTA"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcagcgag gatggagccc gccatcagct ggtgcatgat ccggcctgta agcggagtcc
       61 tcgggcacag cagtcgcgcc ttaaatcttg gcctcactgc atcagtggac caggggtggc
      121 tcagggcagc gctgctgtga tcgtgaaaac aaagaatagg gaaaccaagt ccaggtttgc
      181 aaatctgaga ctttttttgg ttggtgattt aaggttttat tgccgagcaa aatgggagat
      241 gctgaagcag gcaagaagat tttcattcag aaatgtgcgc agtgccacac agtggagaaa
      301 ggtgggaagc acaagacagg gccaaatctc tggggccttt ttggccgaaa gactggacaa
      361 gcaccaggat tttcttacac ggatgcaaac aagaacaaag gtgttatctg gacagaagaa
      421 actttgatgg aatatttgga gaacccgaag aaatacatcc caggcactaa aatgattttt
      481 gctggtatca aaaagaagag tgagagagaa gaccttattc aatatttaaa agaggcaaca
      541 tcctcatgag ttagtattac agtttcaaaa atatgtgacc aatttcaaat gttgtgttta
      601 gtaagaagca tatctgactt tttgatcagc ctaaagtaat tttaacatgt taaaataaag
      661 gttgatgttt ttgattgtgt a
//
