LOCUS       NM_001011954            3427 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus cytochrome b reductase 1 (Cybrd1), mRNA.
ACCESSION   NM_001011954 XM_215749
VERSION     NM_001011954.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Lane,D.J., Merlot,A.M., Huang,M.L., Bae,D.H., Jansson,P.J.,
            Sahni,S., Kalinowski,D.S. and Richardson,D.R.
  TITLE     Cellular iron uptake, trafficking and metabolism: Key molecules and
            mechanisms and their roles in disease
  JOURNAL   Biochim Biophys Acta 1853 (5), 1130-1144 (2015)
   PUBMED   25661197
  REMARK    Review article
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Marciano,R., Santamarina,A.B., de Santana,A.A., Silva Mde,L.,
            Amancio,O.M., do Nascimento,C.M., Oyama,L.M. and de Morais,M.B.
  TITLE     Effects of prebiotic supplementation on the expression of proteins
            regulating iron absorption in anaemic growing rats
  JOURNAL   Br J Nutr 113 (6), 901-908 (2015)
   PUBMED   25745840
  REMARK    GeneRIF: Data suggest that expression of Cybrd1 in intestinal
            mucosa can be regulated by dietary factors; here, expression of
            Cybrd1 in mucosa of duodenum is up-regulated by prebiotic
            supplement, inulin, in iron-deficiency anemia.
REFERENCE   3  (bases 1 to 3427)
  AUTHORS   Loke,S.Y., Siddiqi,N.J., Alhomida,A.S., Kim,H.C. and Ong,W.Y.
  TITLE     Expression and localization of duodenal cytochrome b in the rat
            hippocampus after kainate-induced excitotoxicity
  JOURNAL   Neuroscience 245, 179-190 (2013)
   PUBMED   23597830
  REMARK    GeneRIF: There is an increase in hippocampal expression of duodenal
            Cybrd1 following kainic acid-induced excitotoxicity.
REFERENCE   4  (bases 1 to 3427)
  AUTHORS   Thomas,C.E., Gaffney-Stomberg,E., Sun,B.H., O'Brien,K.O.,
            Kerstetter,J.E. and Insogna,K.L.
  TITLE     Increasing dietary protein acutely augments intestinal iron
            transporter expression and significantly increases iron absorption
            in rats
  JOURNAL   FASEB J 27 (6), 2476-2483 (2013)
   PUBMED   23447582
  REMARK    GeneRIF: Whole-genome profiling reveals that increasing dietary
            protein from 5 to 40% increases duodenal transcript expression of
            divalent metal transporter 1 (DMT1) 3.2-fold, duodenal cytochrome b
            (Dcytb) 1.8-fold, and transferrin receptor (TfR) 1.8-fold.
REFERENCE   5  (bases 1 to 3427)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   6  (bases 1 to 3427)
  AUTHORS   Turi,J.L., Wang,X., McKie,A.T., Nozik-Grayck,E., Mamo,L.B.,
            Crissman,K., Piantadosi,C.A. and Ghio,A.J.
  TITLE     Duodenal cytochrome b: a novel ferrireductase in airway epithelial
            cells
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 291 (2), L272-L280 (2006)
   PUBMED   16510471
  REMARK    GeneRIF: Results suggest that the presence of duodenal cytochrome b
            (Dcytb) in airway epithelial cells and its regulation by iron may
            contribute to pulmonary cytoprotection [Dcytb].
REFERENCE   7  (bases 1 to 3427)
  AUTHORS   Vargas,J.D., Herpers,B., McKie,A.T., Gledhill,S., McDonnell,J., van
            den Heuvel,M., Davies,K.E. and Ponting,C.P.
  TITLE     Stromal cell-derived receptor 2 and cytochrome b561 are functional
            ferric reductases
  JOURNAL   Biochim Biophys Acta 1651 (1-2), 116-123 (2003)
   PUBMED   14499595
REFERENCE   8  (bases 1 to 3427)
  AUTHORS   McKie,A.T., Barrow,D., Latunde-Dada,G.O., Rolfs,A., Sager,G.,
            Mudaly,E., Mudaly,M., Richardson,C., Barlow,D., Bomford,A.,
            Peters,T.J., Raja,K.B., Shirali,S., Hediger,M.A., Farzaneh,F. and
            Simpson,R.J.
  TITLE     An iron-regulated ferric reductase associated with the absorption
            of dietary iron
  JOURNAL   Science 291 (5509), 1755-1759 (2001)
   PUBMED   11230685
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC085768.1.
            
            On Feb 11, 2005 this sequence version replaced XM_215749.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085768.1, CF109699.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q22"
     gene            1..3427
                     /gene="Cybrd1"
                     /gene_synonym="Dcytb"
                     /note="cytochrome b reductase 1"
                     /db_xref="GeneID:295669"
                     /db_xref="RGD:1305740"
     exon            1..198
                     /gene="Cybrd1"
                     /gene_synonym="Dcytb"
                     /inference="alignment:Splign:2.1.0"
     CDS             6..866
                     /gene="Cybrd1"
                     /gene_synonym="Dcytb"
                     /EC_number="7.2.1.3"
                     /note="duodenal cytochrome b"
                     /codon_start=1
                     /product="plasma membrane ascorbate-dependent reductase
                     CYBRD1"
                     /protein_id="NP_001011954.1"
                     /db_xref="GeneID:295669"
                     /db_xref="RGD:1305740"
                     /translation="MAMEGYRGFLGLLVSALLVGFLSVIFVLIWVLHFREGLGWDGGA
                     LEFNWHPVLAVTGFVFIQGIAIIVYRLPWTWKCSKFLMKSIHAGLNAVAAILAIISVV
                     AVFDYHNVRKIPHMYSLHSWVGLTVLILYIQQLVVGFFIFLLPWAPPSLRAIVMPIHV
                     YSGLLLFGTVIATVLMGVTEKLFFVLKNPSYHSFPPEGVFTNTLGLLILVFGALIFWI
                     VTRPQWKRPREPGSIPLQLNGGNADGMEGAIAISSAHNMDAADAELSSEGAARKRTLG
                     LVDTGQRSTM"
     exon            199..407
                     /gene="Cybrd1"
                     /gene_synonym="Dcytb"
                     /inference="alignment:Splign:2.1.0"
     exon            408..562
                     /gene="Cybrd1"
                     /gene_synonym="Dcytb"
                     /inference="alignment:Splign:2.1.0"
     exon            563..3425
                     /gene="Cybrd1"
                     /gene_synonym="Dcytb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgctgatggc catggaaggc taccggggct tcctgggact gctggtgtcc gcgctgctcg
       61 tgggcttcct gtcggtgatc ttcgtgctca tctgggtcct gcacttccga gaggggcttg
      121 gctgggacgg cggcgcgctc gagtttaact ggcacccggt gctcgccgtg accggcttcg
      181 tcttcatcca gggcatcgcc atcatcgtgt acagattgcc atggacctgg aagtgcagca
      241 agttcttgat gaaatccatc cacgccgggt taaacgctgt ggctgccatc ctggccatca
      301 tctcagtcgt ggccgtgttt gattatcaca atgtccgaaa aatcccccac atgtacagtc
      361 tgcacagctg ggttggactg acagttctca tactctacat ccaacagctt gttgtaggct
      421 ttttcatctt tctgcttcca tgggctccgc cctctctccg ggcaatcgtc atgcccatac
      481 atgtgtattc tggactcctc ctctttggaa cagtgattgc cacggtcctc atgggagtga
      541 cggaaaagct gttctttgtc ctgaaaaatc cttcgtacca ttcattccca ccagaaggtg
      601 tttttacaaa taccctgggc cttctgattc tggtatttgg agccctcatc ttttggattg
      661 tcaccaggcc acaatggaaa cgccctaggg aaccaggctc tatccctctt cagctaaatg
      721 gaggcaacgc agacggaatg gagggcgcca tagcaataag ctccgcccac aacatggacg
      781 ctgcagacgc agagttaagc agcgagggag cagcccggaa aagaaccctc ggccttgttg
      841 acactggcca gaggtccacc atgtgaaatg ttgatgcagc cacgtaactt ctcttccaaa
      901 gtcagcccta cgatttagct tctcctacct agccataggc cgatgtatat tgtggtaacc
      961 aaggaggttt tcctgagagc gattgtgttg actgaggcct gtaaacttac caaatgtaaa
     1021 aggacatgat caatataagt tgtgattgtt tcctctttcc tcttgaggac ccgggcgttg
     1081 gatgtacctg ccagtgggtt gtctgatgaa ctggtagcac ccttggcctc tggctatgcc
     1141 ccattaattc ggggagccca gtgatttcag aactaggtag aacctctctt aatctttaaa
     1201 attagatcac aaatacctgg agagggattt ggatcattgt gccacaaaga tattgtgagg
     1261 aaaacatggg ggtggcagcg gtggtggtgc cttaggaaaa tatctagttc cctctccagc
     1321 tgatttggcc tcacacagac tctgaacgtg acagaatata cgagggtgtt ttggagcagt
     1381 ttggttagtt gcagacagct gaagagatct ctggttagac ttggaagctt ttgtaacact
     1441 cagtccagtg ccagtactcc gaggagccgg tatacttcac ggttcagctt ctgaacctct
     1501 tgcaatgcaa acacgctcag gccccataaa cccgccttgt gtccgcagcc tgtgaagggt
     1561 gccctcgaga ttctgttttt attttccaaa gtgcttctct agctcttact atgtgcttta
     1621 caaatattag ctccttcagt ctccagacag actctgcgag acaggtattg taactagccc
     1681 cattttaggt gaaagatctg aagcttggaa aggttataac ttggcccagc atcacacact
     1741 tctttaattg gtggaactga cattcaaacc ctggcggtct ggctcctctg cccaagatcc
     1801 tatatttcac catattcctt cccctgttta ttctaattgg cagcactatg gtctacctag
     1861 gaataaagtt gcagatgaag gctagtggaa ttgttacagg atgctgcctt acctttggtc
     1921 ctcttccaag gggacccctt agatgatggg aaaggcgccg gaaagacttg gatggagatc
     1981 ctggggcatt agccattgcc aacaagccag agcctgcctt gatgctctct aattttaaca
     2041 gggcatctgt cttgccgaca agaagacttg gacgaggata ctcacagcct tcaagccatt
     2101 accatactgg ttcctcggga tcttagcggc tgaacacaat ttggaaacac acatatcctg
     2161 gtctgccctt ccctcggacc ataggcaggt tccttcctga tttctgtgga atttggagtt
     2221 cttagcaggg agctgagatt tagaagtgat tttcacatgg ccagacttct ctctcagctc
     2281 tgatggaaac ctgcagtagt aactgggcca aaacgatgcc tgtagcgggt agtttaggcg
     2341 actgtgctct gagctctcag acagtcgcag gctcgtacct gcccaggagc tctcttcgat
     2401 ccgaggatga aagacacgca cattagctaa tttttaagtt gccttattgg gtgtgtggct
     2461 gagctcttct aagccatcca cggctaccat gcgcttacct ttactcctag ctcaacatct
     2521 ctaaatctac cctcaactta gttgcccgct cactttctgc gaagcccatt ggtcttgctg
     2581 ctcaagatgc tctcaggctg cccttccgtg ggctgctttc cttttccgcc tacattttgg
     2641 aagatctccc ctgcagctct gagtctattc cacctctctc ccccaagtac cttagttcta
     2701 ccttttccac cttctctcct agcctgcaga aacctggaag tcccgcctat ttccgcccag
     2761 ccattgacca ctggcatctt tattgataga tcaaaaacca attgggagca aggaccttta
     2821 gtgtcagtaa acacagattc ccgactaaat caaagcttca aaccactcct ctacagaggc
     2881 ctggtgcaga ttgcctggtt tttggtttct ctgcattttc tgattgaggc acttgggcac
     2941 taaagtgtga gccttgtagt tccagctgct cgagaggctg aacttaaggt acagttcaag
     3001 gccagcctgg gcaactgagt gaaactctct cttaggattt tggtttctct agtaacccaa
     3061 gttggcctct ctcgttgtat aattaagggt gaccttgaag acgtggttct ccttgccttc
     3121 ccaaagctgg atgacaggcg tgtaccacca gccactgtgc cttagcacgg ctccgtcttt
     3181 taaataaata aagccaaacc aaacaagaat tttaagatgg agagaaaaaa cgtgtagttt
     3241 acagagaaat acacttaaca tgtttggcgt gtagcgaaat tccttttagg taacatgatt
     3301 tatttccaaa agaccaaata cggggcgggg ttggggattt agctcagtgg tagagcactt
     3361 gcctagcaag cgcaaggccc tgggttcggt cctcagctcc ggaaaaaaaa aaaaaaaaaa
     3421 aaaaaaa
//
