LOCUS       NM_133427               2252 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), mRNA.
ACCESSION   NM_133427
VERSION     NM_133427.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Larade,K., Jiang,Z.G., Dejam,A., Zhu,H. and Bunn,H.F.
  TITLE     The reductase NCB5OR is responsive to the redox status in
            beta-cells and is not involved in the ER stress response
  JOURNAL   Biochem J 404 (3), 467-476 (2007)
   PUBMED   17343567
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Roma,G.W., Crowley,L.J., Davis,C.A. and Barber,M.J.
  TITLE     Mutagenesis of Glycine 179 modulates both catalytic efficiency and
            reduced pyridine nucleotide specificity in cytochrome b5 reductase
  JOURNAL   Biochemistry 44 (41), 13467-13476 (2005)
   PUBMED   16216070
  REMARK    GeneRIF: G179 was required for both effective NADH/NADPH
            selectivity & to maintain the correct orientation & position of the
            conserved cysteine in the proline-rich 'CGpppM' motif that is
            critical for optimum NADH binding & efficient hydride transfer in
            cb5r.
REFERENCE   3  (bases 1 to 2252)
  AUTHORS   Andersen,G., Wegner,L., Rose,C.S., Xie,J., Zhu,H., Larade,K.,
            Johansen,A., Ek,J., Lauenborg,J., Drivsholm,T., Borch-Johnsen,K.,
            Damm,P., Hansen,T., Bunn,H.F. and Pedersen,O.
  TITLE     Variation in NCB5OR: studies of relationships to type 2 diabetes,
            maturity-onset diabetes of the young, and gestational diabetes
            mellitus
  JOURNAL   Diabetes 53 (11), 2992-2997 (2004)
   PUBMED   15504981
REFERENCE   4  (bases 1 to 2252)
  AUTHORS   Xie,J., Zhu,H., Larade,K., Ladoux,A., Seguritan,A., Chu,M., Ito,S.,
            Bronson,R.T., Leiter,E.H., Zhang,C.Y., Rosen,E.D. and Bunn,H.F.
  TITLE     Absence of a reductase, NCB5OR, causes insulin-deficient diabetes
  JOURNAL   Proc Natl Acad Sci U S A 101 (29), 10750-10755 (2004)
   PUBMED   15247412
REFERENCE   5  (bases 1 to 2252)
  AUTHORS   Zhu,H., Larade,K., Jackson,T.A., Xie,J., Ladoux,A., Acker,H.,
            Berchner-Pfannschmidt,U., Fandrey,J., Cross,A.R.,
            Lukat-Rodgers,G.S., Rodgers,K.R. and Bunn,H.F.
  TITLE     NCB5OR is a novel soluble NAD(P)H reductase localized in the
            endoplasmic reticulum
  JOURNAL   J Biol Chem 279 (29), 30316-30325 (2004)
   PUBMED   15131110
REFERENCE   6  (bases 1 to 2252)
  AUTHORS   Curry,B.J., Roman,S.D., Wallace,C.A., Scott,R., Miriami,E. and
            Aitken,R.J.
  TITLE     Identification and characterization of a novel splice variant of
            mouse and rat cytochrome b5/cytochrome b5 reductase
  JOURNAL   Genomics 83 (3), 425-438 (2004)
   PUBMED   14962668
REFERENCE   7  (bases 1 to 2252)
  AUTHORS   Davis,C.A., Dhawan,I.K., Johnson,M.K. and Barber,M.J.
  TITLE     Heterologous expression of an endogenous rat cytochrome
            b(5)/cytochrome b(5) reductase fusion protein: identification of
            histidines 62 and 85 as the heme axial ligands
  JOURNAL   Arch Biochem Biophys 400 (1), 63-75 (2002)
   PUBMED   11913972
REFERENCE   8  (bases 1 to 2252)
  AUTHORS   Zhu,H., Qiu,H., Yoon,H.W., Huang,S. and Bunn,H.F.
  TITLE     Identification of a cytochrome b-type NAD(P)H oxidoreductase
            ubiquitously expressed in human cells
  JOURNAL   Proc Natl Acad Sci U S A 96 (26), 14742-14747 (1999)
   PUBMED   10611283
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1 and BC080240.1.
            
            On Sep 11, 2012 this sequence version replaced NM_133427.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC080240.1, SRR22582416.1531259.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                JAXUCZ010000008.1  96861089-96861110
            23-2252             BC080240.1         1-2230
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q31"
     gene            1..2252
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /note="cytochrome b5 reductase 4"
                     /db_xref="GeneID:171015"
                     /db_xref="RGD:621834"
     exon            1..214
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..1702
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /EC_number="1.6.2.2"
                     /note="flavohemoprotein b5/b5R; N-terminal cytochrome b5
                     and cytochrome b5 oxidoreductase domain-containing
                     protein; NADPH cytochrome B5 oxidoreductase;
                     flavohemoprotein b5+b5R"
                     /codon_start=1
                     /product="cytochrome b5 reductase 4"
                     /protein_id="NP_596918.3"
                     /db_xref="GeneID:171015"
                     /db_xref="RGD:621834"
                     /translation="MLNVPSQAFPAPGSQQRVASQGRSKVPLKQGRSLMDWIRLTKSG
                     KDFTGLKGGLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGA
                     DGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQVTDTLP
                     REGPSSPSYDWFQTESSVTIVIYTKQKNINLDSVIVDLQDDSLRAEAVIKDHSYLIHI
                     GLSHEVQENFSVRVIENVGKIEIVLQKKETVSWKCLGDPLEKHDSFIPKKDTGLYYRQ
                     CQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSESLLS
                     DFKEPVLSPNKYIYFLIKIYPAGLFTPELDRLQIGDFVSVSGPEGNFKVSKLQEVEDL
                     FLLAAGTGFTPMVTVLNHALTHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALKDKRFH
                     VEYVLSAPSPEWNGKQGHVSRALLSEFLQRSLENSKVFLCICGPTPFTDEGIRLLHDL
                     NFSDDEIHGFTA"
     exon            215..368
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            369..469
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            470..551
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            552..581
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            582..642
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            643..700
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            701..794
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            795..827
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            828..950
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1091
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1244
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1395
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1482
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1647
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..2230
                     /gene="Cyb5r4"
                     /gene_synonym="b5&b5R; b5+b5R; cb5/cb5R; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tggcgacccc ggaagtgggt cgggggcttg gcctcggcca ggccaaggag ccggcacaga
       61 tagtggtggc cgctggagtc cggccaaccg agcctgtccg gtggcggagg ccggatgcgg
      121 cccgagccgg gggcccagga tgctgaacgt cccttcccag gctttcccgg cccccggctc
      181 ccagcagcgc gttgcctccc aggggcgtag caaggtacct ttaaaacaag gcaggagtct
      241 tatggattgg atccgattga ccaaaagtgg aaaggacttc acgggattga agggcgggtt
      301 aattgaagta acagaagaag aacttaagaa gcacaacaag aaagacgact gttggatatg
      361 cataagaggg tttgtttaca atgtcagccc atatatggag tatcatcctg gtggggagga
      421 tgagcttatg agagccgcag gagcagatgg aacggatctt tttaatgagg tgcatcgttg
      481 ggtcaattat gaatccatgc tgaaagagtg cctggttggc aggatggctg tgaagcccgc
      541 tgttccgaaa gactgtcatg aaggaaagag agtcttaaat ggcatgcttc ccaagagcca
      601 agtgacagac acactgccca gggaaggccc cagttctcca agctatgatt ggttccaaac
      661 agagtcttca gtgaccattg ttatatacac caagcagaag aatatcaatt tagactcagt
      721 aatcgttgac ctccaggatg actccttgag agcagaagca gtcatcaagg atcattctta
      781 tcttatacat attgggttaa gccatgaagt tcaagaaaat ttttctgtac gggttattga
      841 gaatgttgga aaaatagaga ttgtcctaca aaagaaagaa actgtttctt ggaaatgtct
      901 tggtgatccc ctggagaaac atgattcttt tattccaaag aaagatacag gcttgtacta
      961 tagacagtgc cagttaattt ccaaggaaga cgttactcat gacacaaggc tcttctgtct
     1021 gatgctgcct cccagtactc accttcaggt gccagttggg caacatgttt acctcaagct
     1081 cagtgtcaca ggtgcagaaa tagtaaagcc atatacaccc gtgtctgagt ccttactctc
     1141 agattttaaa gaaccagttc tttccccaaa taagtacatc tactttttga tcaaaatcta
     1201 tcctgctgga ctcttcacgc cagaacttga tcgtcttcag attggagatt ttgtttctgt
     1261 aagtggtcct gagggcaatt ttaaagtttc caaactccag gaagtggaag atctcttttt
     1321 attggcagcc ggaacaggct tcacaccaat ggttactgta ctgaatcacg ctttaactca
     1381 catgtccagt ctcaggaagg tgaagttaat gttcttcaat aaaacagagg atgacataat
     1441 ttggagatgc cagctagaga aattggcatt aaaagataaa agatttcatg ttgaatatgt
     1501 tctctcggca ccttccccag aatggaatgg caaacaggga cacgtgtcac gagctcttct
     1561 ctctgaattt ttgcaacgaa gtttagaaaa ttctaaagtc ttcctttgca tctgtggacc
     1621 aaccccattt acagatgagg ggataaggtt gctgcatgat cttaactttt ccgatgatga
     1681 gattcatggt tttacagcat aataaaaaga tgccattgtc ttttattcca ctagtttata
     1741 cttgtgattg cctagggttt tttaaaagaa tttttgtatg tacgaaaggt taatcagaat
     1801 ccaggcttcc gtttctgcaa tgaagtcatg tgtcttcagt acaggtaact tcttggttta
     1861 atgtttgtac cgtgacttac ttttctgctg atgcctgacc tgaaacttca gtcatgacag
     1921 caggtgcata caagagcctt tgttatgagc tgcaagtttc cttaccactg aaattgtatc
     1981 actgttctgc agtaaacact tgtattttat gacatgataa actaagcaat cattttggtc
     2041 actttttatg ctgcaagata tcttctgagc aatacagatt aagttttaca ctgcactgtt
     2101 cactcaggaa tcgatggtgt atatccttat tagtcttaac acacagaatg ctgtaactat
     2161 ttgtggtcag gttttgtggg tgtccatggc attgttacag atagaataaa actatacttc
     2221 atacttttta aaaaaaaaaa aaaaaaaaaa aa
//
