LOCUS       NM_138877               1893 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Rattus norvegicus cytochrome b5 reductase 3 (Cyb5r3), mRNA.
ACCESSION   NM_138877
VERSION     NM_138877.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Diaz-Ruiz,A., Lanasa,M., Garcia,J., Mora,H., Fan,F.,
            Martin-Montalvo,A., Di Francesco,A., Calvo-Rubio,M.,
            Salvador-Pascual,A., Aon,M.A., Fishbein,K.W., Pearson,K.J.,
            Villalba,J.M., Navas,P., Bernier,M. and de Cabo,R.
  TITLE     Overexpression of CYB5R3 and NQO1, two NAD+ -producing enzymes,
            mimics aspects of caloric restriction
  JOURNAL   Aging Cell 17 (4), e12767 (2018)
   PUBMED   29706024
  REMARK    GeneRIF: results highlight the importance of CYB5R3 and NQO1, two
            NAD(+) producers, for the promotion of health and extended lifespan
REFERENCE   3  (bases 1 to 1893)
  AUTHORS   Samhan-Arias,A.K., Lopez-Sanchez,C., Marques-da-Silva,D., Lagoa,R.,
            Garcia-Lopez,V., Garcia-Martinez,V. and Gutierrez-Merino,C.
  TITLE     High expression of cytochrome b 5 reductase isoform 3/cytochrome b
            5 system in the cerebellum and pyramidal neurons of adult rat brain
  JOURNAL   Brain Struct Funct 221 (4), 2147-2162 (2016)
   PUBMED   25850901
  REMARK    GeneRIF: Results point out the enrichment of cytochrome b 5
            reductase isoform 3/cytochrome b 5 system in neuronal cells and
            structures of the cerebellum and brain stem.
REFERENCE   4  (bases 1 to 1893)
  AUTHORS   Lund,R.R., Leth-Larsen,R., Caterino,T.D., Terp,M.G., Nissen,J.,
            Laenkholm,A.V., Jensen,O.N. and Ditzel,H.J.
  TITLE     NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis
            Formation and Is a Prognostic Marker of Disease-Free and Overall
            Survival in Estrogen Receptor-Negative Breast Cancer
  JOURNAL   Mol Cell Proteomics 14 (11), 2988-2999 (2015)
   PUBMED   26351264
REFERENCE   5  (bases 1 to 1893)
  AUTHORS   Blanke,K.L., Sacco,J.C., Millikan,R.C., Olshan,A.F., Luo,J. and
            Trepanier,L.A.
  TITLE     Polymorphisms in the carcinogen detoxification genes CYB5A and
            CYB5R3 and breast cancer risk in African American women
  JOURNAL   Cancer Causes Control 25 (11), 1513-1521 (2014)
   PUBMED   25225034
REFERENCE   6  (bases 1 to 1893)
  AUTHORS   Mota Vieira,L., Kaplan,J.C., Kahn,A. and Leroux,A.
  TITLE     Heterogeneity of the rat NADH-cytochrome-b5-reductase transcripts
            resulting from multiple alternative first exons
  JOURNAL   Eur J Biochem 220 (3), 729-737 (1994)
   PUBMED   8143727
REFERENCE   7  (bases 1 to 1893)
  AUTHORS   Pietrini,G., Aggujaro,D., Carrera,P., Malyszko,J., Vitale,A. and
            Borgese,N.
  TITLE     A single mRNA, transcribed from an alternative, erythroid-specific,
            promoter, codes for two non-myristylated forms of NADH-cytochrome
            b5 reductase
  JOURNAL   J Cell Biol 117 (5), 975-986 (1992)
   PUBMED   1577871
REFERENCE   8  (bases 1 to 1893)
  AUTHORS   Zenno,S., Hattori,M., Misumi,Y., Yubisui,T. and Sakaki,Y.
  TITLE     Molecular cloning of a cDNA encoding rat NADH-cytochrome b5
            reductase and the corresponding gene
  JOURNAL   J Biochem 107 (6), 810-816 (1990)
   PUBMED   2391344
REFERENCE   9  (bases 1 to 1893)
  AUTHORS   Murakami,K., Yubisui,T., Takeshita,M. and Miyata,T.
  TITLE     The NH2-terminal structures of human and rat liver microsomal
            NADH-cytochrome b5 reductases
  JOURNAL   J Biochem 105 (2), 312-317 (1989)
   PUBMED   2498303
REFERENCE   10 (bases 1 to 1893)
  AUTHORS   Pietrini,G., Carrera,P. and Borgese,N.
  TITLE     Two transcripts encode rat cytochrome b5 reductase
  JOURNAL   Proc Natl Acad Sci U S A 85 (19), 7246-7250 (1988)
   PUBMED   3174630
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from D00636.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062066.1, J03867.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..1893
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /note="cytochrome b5 reductase 3"
                     /db_xref="GeneID:25035"
                     /db_xref="RGD:2502"
     exon            1..59
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..944
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /EC_number="1.6.2.2"
                     /note="B5R; diaphorase-1; Diaphorase (NADH) (cytochrome
                     b-5 reductase)"
                     /codon_start=1
                     /product="NADH-cytochrome b5 reductase 3"
                     /protein_id="NP_620232.1"
                     /db_xref="GeneID:25035"
                     /db_xref="RGD:2502"
                     /translation="MGAQLSTLSRVVLSPVWFVYSLFMKLFQRSSPAITLENPDIKYP
                     LRLIDKEIISHDTRRFRFALPSPQHILGLPIGQHIYLSTRIDGNLVIRPYTPVSSDDD
                     KGFVDLVVKVYFKDTHPKFPAGGKMSQYLENMNIGDTIEFRGPNGLLVYQGKGKFAIR
                     ADKKSNPVVRTVKSVGMIAGGTGITPMLQVIRAVLKDPNDHTVCYLLFANQSEKDILL
                     RPELEELRNEHSSRFKLWYTVDKAPDAWDYSQGFVNEEMIRDHLPPPGEETLILMCGP
                     PPMIQFACLPNLERVGHPKERCFTF"
     exon            60..191
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     exon            192..264
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     exon            265..371
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     exon            372..501
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     exon            502..585
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     exon            586..671
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     exon            672..771
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     exon            772..1893
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1893
                     /gene="Cyb5r3"
                     /gene_synonym="Dia1; Nadhcb5; RNNADHCB5"
                     /note="12 a nucleotides"
ORIGIN      
        1 gggtgtagaa cggtgccacc actgtcttct tcgccaccat gggggcccag ctgagcacgt
       61 tgagccgagt ggtactctcc ccggtctggt tcgtctacag cctcttcatg aagctgtttc
      121 agcgctcctc accggccatc accctcgaga accccgacat caagtaccct ctgcggctca
      181 tcgacaagga gattatcagc cacgacactc ggcgcttccg atttgcactc ccttcgcccc
      241 agcacatcct gggccttcct atcggccagc acatctacct ctccaccagg atcgatggca
      301 acttggtcat tcgtccctac acccctgtgt ctagtgatga tgacaagggc tttgtggact
      361 tggtggtcaa ggtttacttc aaggacacgc atcccaagtt tccagctgga gggaaaatgt
      421 ctcagtacct ggaaaacatg aatattggag acaccattga attccggggc cccaatgggc
      481 tactggtcta ccagggcaaa gggaagttcg ccatccgtgc agacaagaag tccaaccctg
      541 ttgtcaggac ggtgaagtct gtaggcatga ttgcaggagg gacaggcatc accccaatgc
      601 tgcaggtgat ccgagccgtc ttgaaggacc cgaacgacca cactgtgtgc tatctgctct
      661 tcgccaacca gtccgagaaa gacatcctgc tgcggcctga gctggaggaa ctgaggaacg
      721 aacattcttc tcgcttcaag ctctggtaca cagtggacaa agcccccgat gcctgggact
      781 atagccaagg cttcgtgaat gaggagatga tcagggacca tcttccacct cctggggagg
      841 agacactgat actgatgtgt ggacccccac cgatgatcca gtttgcctgt ttgccaaacc
      901 tggagcgtgt gggccatccc aaggagcgat gcttcacctt ctgatggctg gatgctggcc
      961 actcccatgc ctgctgctca cgcactcacc acaaccacct tccacccctt ccttccccac
     1021 tactgtcctt taccctgaca tatgcccaca tccatgctgg ggcctgggtt cagcctggcc
     1081 tgcccagccc tggtcatcca gctgtactgg cccctgaggg gcccctttgg gagcaggcct
     1141 gtgtatcagg tggcttctgt tgaccacttt ctgaataggc ttctgtctgg tactaactgg
     1201 ccattaccag agatggtcca tgaccacccc tttatacaca cacacataca catacagaga
     1261 cagagagaca gagagacaga gatagacaga gatagacaga gagacagata cagacataga
     1321 gacagacaga gacagagaga gaggagtcag agagctgtta gtaccatgtc tacccgttac
     1381 catggaccgc aatgtgtagt aaggaaatga aaggtaaaca gttatctaca gtcctaggaa
     1441 ccatgcctgt cacctaacca cctactgcct taggctatgg cctggtctgt gcccctaaac
     1501 actacattag acagtgacac ccagaggttc tcttaggagg gtgtctggca gtgacaggcc
     1561 agcccccact tgctggccag gagttccttg ggtcagcacg tggacccttc caaaacccca
     1621 cacactgccc ctcccctcca tgatgccttg agcaccctaa gttgtcagcc caacagagtt
     1681 tggtagacct tggggtacct ggcttgtttc tccatcccat acctccctcc catctggttc
     1741 ccagcctggg gggttctgag cagagcctct tttctcggag acagacccgg tgcctggcgc
     1801 tgccttcagc agacagcaga cagcctcctg cacactggct ttttttagtc atttatgggc
     1861 aaaatgagtt aaaataaaac tttgcaaatc ctg
//
