LOCUS       NM_030586               2745 bp    mRNA    linear   ROD 31-MAY-2024
DEFINITION  Rattus norvegicus cytochrome b5 type B (Cyb5b), mRNA.
ACCESSION   NM_030586
VERSION     NM_030586.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Neve,E.P., Nordling,A., Andersson,T.B., Hellman,U., Diczfalusy,U.,
            Johansson,I. and Ingelman-Sundberg,M.
  TITLE     Amidoxime reductase system containing cytochrome b5 type B (CYB5B)
            and MOSC2 is of importance for lipid synthesis in adipocyte
            mitochondria
  JOURNAL   J Biol Chem 287 (9), 6307-6317 (2012)
   PUBMED   22203676
  REMARK    GeneRIF: The presence of a novel reductive enzyme system of
            importance for lipid synthesis that is exclusively localized to the
            outer mitochondrial membrane and is composed of CYB5B, MOSC2, and a
            third unknown component (a CYB5B reductase).
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Parthasarathy,S., Altuve,A., Terzyan,S., Zhang,X., Kuczera,K.,
            Rivera,M. and Benson,D.R.
  TITLE     Accommodating a nonconservative internal mutation by water-mediated
            hydrogen bonding between beta-sheet strands: a comparison of human
            and rat type B (mitochondrial) cytochrome b5
  JOURNAL   Biochemistry 50 (24), 5544-5554 (2011)
   PUBMED   21574570
  REMARK    GeneRIF: comparison of X-ray crystal structures of human and rat
            CYB5B reveals a striking difference in packing involving the
            five-strand beta-sheet, which can be attributed to fully buried
            residue 21 in strand beta4
REFERENCE   3  (bases 1 to 2745)
  AUTHORS   Wahl,B., Reichmann,D., Niks,D., Krompholz,N., Havemeyer,A.,
            Clement,B., Messerschmidt,T., Rothkegel,M., Biester,H., Hille,R.,
            Mendel,R.R. and Bittner,F.
  TITLE     Biochemical and spectroscopic characterization of the human
            mitochondrial amidoxime reducing components hmARC-1 and hmARC-2
            suggests the existence of a new molybdenum enzyme family in
            eukaryotes
  JOURNAL   J Biol Chem 285 (48), 37847-37859 (2010)
   PUBMED   20861021
REFERENCE   4  (bases 1 to 2745)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   5  (bases 1 to 2745)
  AUTHORS   Cheng,Q., Benson,D.R., Rivera,M. and Kuczera,K.
  TITLE     Influence of point mutations on the flexibility of cytochrome b5:
            molecular dynamics simulations of holoproteins
  JOURNAL   Biopolymers 83 (3), 297-312 (2006)
   PUBMED   16807901
  REMARK    GeneRIF: simulations provided qualitative microscopic explanations
            of many of the differences in physical properties between outer
            mitochondrial membrane CYB5 isoform and microsomal CYB5 and two
            mutants in terms of localized changes in structure and flexibility
REFERENCE   6  (bases 1 to 2745)
  AUTHORS   Wirtz,M., Oganesyan,V., Zhang,X., Studer,J. and Rivera,M.
  TITLE     Modulation of redox potential in electron transfer proteins:
            effects of complex formation on the active site microenvironment of
            cytochrome b5
  JOURNAL   Faraday Discuss (116), 221-34 (2000)
   PUBMED   11197480
REFERENCE   7  (bases 1 to 2745)
  AUTHORS   Rivera,M., Seetharaman,R., Girdhar,D., Wirtz,M., Zhang,X., Wang,X.
            and White,S.
  TITLE     The reduction potential of cytochrome b5 is modulated by its
            exposed heme edge
  JOURNAL   Biochemistry 37 (6), 1485-1494 (1998)
   PUBMED   9484218
REFERENCE   8  (bases 1 to 2745)
  AUTHORS   Rodriguez-Maranon,M.J., Qiu,F., Stark,R.E., White,S.P., Zhang,X.,
            Foundling,S.I., Rodriguez,V., Schilling,C.L. 3rd, Bunce,R.A. and
            Rivera,M.
  TITLE     13C NMR spectroscopic and X-ray crystallographic study of the role
            played by mitochondrial cytochrome b5 heme propionates in the
            electrostatic binding to cytochrome c
  JOURNAL   Biochemistry 35 (50), 16378-16390 (1996)
   PUBMED   8973214
REFERENCE   9  (bases 1 to 2745)
  AUTHORS   De Silvestris,M., D'Arrigo,A. and Borgese,N.
  TITLE     The targeting information of the mitochondrial outer membrane
            isoform of cytochrome b5 is contained within the carboxyl-terminal
            region
  JOURNAL   FEBS Lett 370 (1-2), 69-74 (1995)
   PUBMED   7649306
REFERENCE   10 (bases 1 to 2745)
  AUTHORS   Lederer,F., Ghrir,R., Guiard,B., Cortial,S. and Ito,A.
  TITLE     Two homologous cytochromes b5 in a single cell
  JOURNAL   Eur J Biochem 132 (1), 95-102 (1983)
   PUBMED   6840088
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC072535.1.
            
            On Jun 20, 2004 this sequence version replaced NM_030586.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC072535.1, EV775749.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..2745
                     /gene="Cyb5b"
                     /gene_synonym="Cyb5m; omb5"
                     /note="cytochrome b5 type B"
                     /db_xref="GeneID:80773"
                     /db_xref="RGD:621551"
     exon            1..168
                     /gene="Cyb5b"
                     /gene_synonym="Cyb5m; omb5"
                     /inference="alignment:Splign:2.1.0"
     CDS             7..447
                     /gene="Cyb5b"
                     /gene_synonym="Cyb5m; omb5"
                     /note="cytochrome b5 type B (outer mitochondrial
                     membrane)"
                     /codon_start=1
                     /product="cytochrome b5 type B precursor"
                     /protein_id="NP_085075.1"
                     /db_xref="GeneID:80773"
                     /db_xref="RGD:621551"
                     /translation="MATPEASGSGRNGQGSDPAVTYYRLEEVAKRNTAEETWMVIHGR
                     VYDITRFLSEHPGGEEVLLEQAGADATESFEDVGHSPDAREMLKQYYIGDVHPNDLKP
                     KDGDKDPSKNNSCQSSWAYWIVPIVGAILIGFLYRHFWADSKSS"
     exon            169..297
                     /gene="Cyb5b"
                     /gene_synonym="Cyb5m; omb5"
                     /inference="alignment:Splign:2.1.0"
     exon            298..327
                     /gene="Cyb5b"
                     /gene_synonym="Cyb5m; omb5"
                     /inference="alignment:Splign:2.1.0"
     exon            328..356
                     /gene="Cyb5b"
                     /gene_synonym="Cyb5m; omb5"
                     /inference="alignment:Splign:2.1.0"
     exon            357..2726
                     /gene="Cyb5b"
                     /gene_synonym="Cyb5m; omb5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gatccaatgg cgaccccaga ggccagcggc agcggcagga atggccaggg ctccgacccc
       61 gccgtcacct actaccggct ggaggaggtg gcgaagcgaa ataccgcgga ggagacgtgg
      121 atggtgatcc acgggcgagt ctacgatatc acccgcttcc tcagcgagca tcctggtgga
      181 gaagaggttc tactggaaca agctggtgcc gatgcaactg aaagctttga agatgtcggc
      241 cactcccctg atgccaggga gatgctaaag cagtactaca ttggtgatgt ccatccgaat
      301 gaccttaaac ctaaagatgg tgacaaggac ccttcaaaaa acaattcatg ccaaagttcc
      361 tgggcatatt ggattgtccc catcgtgggt gctattctta taggtttcct gtatcgtcac
      421 ttctgggctg acagcaaatc ctcctgagga ggccacgttg aagtgtcgga gcacatctcc
      481 tgggagtgag aggtggagac ttgtgtggag gctgcagggt gctccttcct tggatcctgc
      541 cagttgtttt ctttcccctt ggagccaaga tggttgacca gatatctatc ctccttcaat
      601 gcaggaccag aatctgtcca tcagcctgag cctgacgccc aaagcacctg ctcacgttgt
      661 gtccttgtca gggtttcttc tcactggttc ccactcattc ccttcatttc aacagttgat
      721 gccaatgact tagagttcct ttgttgtagt gacatggcct ttggggacag tacatgattt
      781 ctgatacttt gaattcacag tagacatagt taagggaaca gcggtctagt tttgagtctt
      841 tccccatttt tcttttaagt aaattttttt aattctgatt taattttgtc attcatgatg
      901 ataagggatt taattcatga agttgtgtag ctcttgtttc aaacacagct gccaaatgat
      961 cttacttatg attgttagaa gtaaggagcc agcaaaatgg ctcactggat aaaagcaatt
     1021 gcagaaaaac caatgacctg tgtttgagtc cctgtaccca cagggtagaa ggaagagaac
     1081 cagttcccaa aagttgtcct ctgacttcca catgtgtgct gtggtctttg tgtacacaca
     1141 cacacacaca cacacacaca cacacacacc ccagatgtaa attttaaaaa tagaatacaa
     1201 cagtggttgt cttcctccca gatagtgtag ctgatggccc ctcctgtctt ttttcccatg
     1261 agtatgtcac caaactgtgc atccctttga ttttttaggg gtataagagc tgaagaaggg
     1321 ccaacttgag aataatctca tttacaaaaa gaaccaaaca ctacttatag gctagcagtc
     1381 taaaaagtga ctggcatgca ctctctggtc tgcccttggt ccctgaccgt cttccgtcct
     1441 ggctgtcttt actggtggaa gttgaaggtg tgggtatcca tgggacttct gtccggtccg
     1501 gtccgcgggg cttcacaaga ggcttgctgc ttttctgttt actgattagc aagtttctgt
     1561 ttccctgttt gaaagtaaga gactcaagtt tgatcttccg tttctctcag taaataactc
     1621 acatgtaaat gtcattgtcc acacaccatt tctgatcggg agaccatgcc aagagacttc
     1681 tgttttcatt tgggctctac ctccatggct ccaggttggt gattttgtcc tacttttgag
     1741 gataatgata aatctggaag cttttttccc ccttggatat gaatttcatt ggaaagtagg
     1801 cagtcattta aaattccttt tcttactggt ccaggcattt tcctggcatc tctgactgta
     1861 ttctctctcc ctgtggcagt gcccgacagt acccagctgt ttcttagttt ctatgatttt
     1921 ccttcacatg tttggtcatt gctagtcagc ttctgagctg gtgtcacatc cagtaggaag
     1981 ccatggaatc ccagcgggac cagagatatt gggaagtgtc ctcctgcttc ccttctaccc
     2041 tgccaggggc ttgacaagac aggacactac acagtacacc agtctgccca agcaaactgt
     2101 tttaaaaaaa gttcagccca ggacttttat cctgtgattt tagcagttga atgaattcag
     2161 aagtcatcaa aataacatta gccttttcct tgcatacttg gatatgatat tttagagtga
     2221 tttctaggtg acttttatac atcgaagatg aaggcttaac ctttcatttt tagaacgtct
     2281 gtaatactgt gtcacttatt gacagcgttg tcactctgta acagcagtgg catgcccagc
     2341 gcacctccac ttaaaggcag ggaaggcttg tgtgcaggga ggctgggatc cacttttgag
     2401 gatagccaag tcacatggag tatatggagt atgctgggta agaggaaagg acgagagtgt
     2461 gggatggggg cgttccagcc aataccaaac ctgatggtgt gcctagtgaa aacacctggc
     2521 ataggaaatt aggtaaagaa ctacattttt ggctgggcat gttgatgcac acctttaatc
     2581 ccagtataag agaagcaggg gctggaaaat ctcttgagtt attgccagcc tgctctacat
     2641 agcaagttct aggacagtca gggctacatg gagagaccat gtcacaacca ctccaccaaa
     2701 taaaagaact gtattttcaa gtaaaaaaaa aaaaaaaaaa aaaaa
//
