LOCUS       NM_001399213            3798 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus cullin 2 (Cul2), mRNA.
ACCESSION   NM_001399213 XM_006254072
VERSION     NM_001399213.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Choy,M.K., Movassagh,M., Bennett,M.R. and Foo,R.S.
  TITLE     PKB/Akt activation inhibits p53-mediated HIF1A degradation that is
            independent of MDM2
  JOURNAL   J Cell Physiol 222 (3), 635-639 (2010)
   PUBMED   19950214
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Semenza,G.L.
  TITLE     Regulation of oxygen homeostasis by hypoxia-inducible factor 1
  JOURNAL   Physiology (Bethesda) 24, 97-106 (2009)
   PUBMED   19364912
  REMARK    Review article
REFERENCE   3  (bases 1 to 3798)
  AUTHORS   Ferguson,J.E. 3rd, Wu,Y., Smith,K., Charles,P., Powers,K., Wang,H.
            and Patterson,C.
  TITLE     ASB4 is a hydroxylation substrate of FIH and promotes vascular
            differentiation via an oxygen-dependent mechanism
  JOURNAL   Mol Cell Biol 27 (18), 6407-6419 (2007)
   PUBMED   17636018
REFERENCE   4  (bases 1 to 3798)
  AUTHORS   Mekhail,K., Khacho,M., Carrigan,A., Hache,R.R., Gunaratnam,L. and
            Lee,S.
  TITLE     Regulation of ubiquitin ligase dynamics by the nucleolus
  JOURNAL   J Cell Biol 170 (5), 733-744 (2005)
   PUBMED   16129783
REFERENCE   5  (bases 1 to 3798)
  AUTHORS   Kamura,T., Maenaka,K., Kotoshiba,S., Matsumoto,M., Kohda,D.,
            Conaway,R.C., Conaway,J.W. and Nakayama,K.I.
  TITLE     VHL-box and SOCS-box domains determine binding specificity for
            Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases
  JOURNAL   Genes Dev 18 (24), 3055-3065 (2004)
   PUBMED   15601820
REFERENCE   6  (bases 1 to 3798)
  AUTHORS   Pan,Z.Q., Kentsis,A., Dias,D.C., Yamoah,K. and Wu,K.
  TITLE     Nedd8 on cullin: building an expressway to protein destruction
  JOURNAL   Oncogene 23 (11), 1985-1997 (2004)
   PUBMED   15021886
  REMARK    Review article
REFERENCE   7  (bases 1 to 3798)
  AUTHORS   Ohh,M., Kim,W.Y., Moslehi,J.J., Chen,Y., Chau,V., Read,M.A. and
            Kaelin,W.G. Jr.
  TITLE     An intact NEDD8 pathway is required for Cullin-dependent
            ubiquitylation in mammalian cells
  JOURNAL   EMBO Rep 3 (2), 177-182 (2002)
   PUBMED   11818338
REFERENCE   8  (bases 1 to 3798)
  AUTHORS   Kamura,T., Koepp,D.M., Conrad,M.N., Skowyra,D., Moreland,R.J.,
            Iliopoulos,O., Lane,W.S., Kaelin,W.G. Jr., Elledge,S.J.,
            Conaway,R.C., Harper,J.W. and Conaway,J.W.
  TITLE     Rbx1, a component of the VHL tumor suppressor complex and SCF
            ubiquitin ligase
  JOURNAL   Science 284 (5414), 657-661 (1999)
   PUBMED   10213691
REFERENCE   9  (bases 1 to 3798)
  AUTHORS   Pause,A., Lee,S., Worrell,R.A., Chen,D.Y., Burgess,W.H.,
            Linehan,W.M. and Klausner,R.D.
  TITLE     The von Hippel-Lindau tumor-suppressor gene product forms a stable
            complex with human CUL-2, a member of the Cdc53 family of proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (6), 2156-2161 (1997)
   PUBMED   9122164
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Jan 4, 2022 this sequence version replaced XM_006254072.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643299.61137.1,
                                           SRR26360190.265167.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               JAXUCZ010000017.1  58904977-58905132   c
            157-297             JAXUCZ010000017.1  58891235-58891375   c
            298-400             JAXUCZ010000017.1  58887283-58887385   c
            401-495             JAXUCZ010000017.1  58885905-58885999   c
            496-601             JAXUCZ010000017.1  58882483-58882588   c
            602-684             JAXUCZ010000017.1  58881095-58881177   c
            685-781             JAXUCZ010000017.1  58879932-58880028   c
            782-892             JAXUCZ010000017.1  58879730-58879840   c
            893-1055            JAXUCZ010000017.1  58877669-58877831   c
            1056-1180           JAXUCZ010000017.1  58875844-58875968   c
            1181-1288           JAXUCZ010000017.1  58874826-58874933   c
            1289-1348           JAXUCZ010000017.1  58873791-58873850   c
            1349-1564           JAXUCZ010000017.1  58872937-58873152   c
            1565-1717           JAXUCZ010000017.1  58872484-58872636   c
            1718-1795           JAXUCZ010000017.1  58872153-58872230   c
            1796-1862           JAXUCZ010000017.1  58869507-58869573   c
            1863-2065           JAXUCZ010000017.1  58867940-58868142   c
            2066-2167           JAXUCZ010000017.1  58867461-58867562   c
            2168-2284           JAXUCZ010000017.1  58866501-58866617   c
            2285-3798           JAXUCZ010000017.1  58864274-58865787   c
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.1"
     gene            1..3798
                     /gene="Cul2"
                     /note="cullin 2"
                     /db_xref="GeneID:361258"
                     /db_xref="RGD:1310644"
     exon            1..156
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            157..297
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..2416
                     /gene="Cul2"
                     /codon_start=1
                     /product="cullin-2"
                     /protein_id="NP_001386142.1"
                     /db_xref="GeneID:361258"
                     /db_xref="RGD:1310644"
                     /translation="MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYA
                     LCVAYPEPLGERLYAETKIFLENHVRHLYKRVLESEEQVLVMYHRYWEEYSKGADYMD
                     CLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQNILI
                     RMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQGIFVSPFLTETGEY
                     YKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQ
                     FLHSECHSIIRHERKNDMANMYVLLRAVSSGLPHMIEELQRHIHDEGLRATSNLTQEH
                     MPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLA
                     KYCDNLLKKSAKGMTENEVEDKLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMS
                     MDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIRNQDTVIDLGISFQ
                     IYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKM
                     NYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMIN
                     HDSEKEDIDAESSFSLNMSFSSKRTKFKITTSMQKDTPQELEQTRSAVDEDRKMYLQA
                     AIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADE
                     YSYVA"
     exon            298..400
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            401..495
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            496..601
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            602..684
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            685..781
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            782..892
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1055
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1180
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1288
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1348
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1564
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1717
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1795
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1862
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..2065
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2167
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2284
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..3798
                     /gene="Cul2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaggcggcct cggcgcacag taatggcagc gctgtgagcc ggggaacgcg agctgacagc
       61 cgccgccgcc caccctcgga gccgggggct tcgtccttca cgcctgcggg ttgcctccct
      121 ccccgggctc ctgccctcgc cctgcttttg cagaaggttt taacactaaa cttgcacaat
      181 gtctttaaaa ccaagagtag tggattttga tgagacgtgg aacaagctgc taactacaat
      241 caaggctgtc gttatgttgg aatatgtaga gagagcaaca tggaacgacc gcttctcaga
      301 catctatgct ttatgtgtgg cctatcctga accacttgga gaaagacttt atgcggaaac
      361 aaagattttt ttggaaaatc acgttcggca tttatataag agagttctgg agtctgaaga
      421 acaagtgctt gttatgtatc ataggtactg ggaagaatac agcaagggtg ctgactatat
      481 ggactgctta tataggtatc tcaataccca gtatattaaa aagaataaat tgacggaagc
      541 tgatttacag tatggctatg gtggagtaga tatgaatgaa ccacttatgg aaataggaga
      601 gctagcattg gatatgtgga ggaagttaat ggttgaaccc cttcagaaca tcctcatccg
      661 gatgctgctt cgagaaatta aaaatgatcg tggtggagaa gacccaaacc agaaagtaat
      721 ccatggggtt attaactcct ttgttcatgt tgaacagtat aagaaaaaat tccccttaaa
      781 gttttatcag ggaatatttg tgtctccctt tctgaccgaa acgggagaat attacaagca
      841 agaagcttca aatttattac aagaatcaaa ttgttcacag tatatggaaa aggttctagg
      901 tagattaaaa gatgaagaaa ttagatgtcg aaaatatttg catccaagtt catatacgaa
      961 agtgattcac gagtgtcagc agcggatggt ggcagaccac ttgcagttct tacactcaga
     1021 gtgccacagc attattcggc acgagaggaa gaacgacatg gcaaatatgt acgtcttact
     1081 tcgtgctgtg tccagtggtt tacctcatat gattgaggag ctacagagac acatccacga
     1141 tgagggcctt cgagcgacca gtaaccttac tcaggaacat atgccaacac tatttgtgga
     1201 atcagttttg gaagtgcacg gtaaattcgt tcaacttatc aacactgttt tgaatggtga
     1261 tcagcatttt atgagtgctt tagataaggc ccttacgtct gttgtaaatt acagagaacc
     1321 caagtctgtt tgcaaagcac ctgaactgct tgctaagtac tgtgacaact tgctaaagaa
     1381 gtcagcaaag gggatgacgg agaatgaagt agaggacaag cttaccagct tcatcacagt
     1441 tttcaagtac atcgatgaca aggacgtctt tcagaagttc tatgcaagaa tgctagcaaa
     1501 acgtctaatt cacgggctgt ctatgtctat ggattctgaa gaagccatga tcaataaatt
     1561 aaagcaagcc tgtgggtatg agtttaccag caagctgcat cggatgtaca cagacatgag
     1621 tgtcagcgcc gacctcaaca acaagttcaa caatttcatc agaaaccaag acactgtgat
     1681 cgatttgggg attagtttcc aaatatatgt ccttcaggct ggtgcgtggc ctctcaccca
     1741 ggctccgtcg tccacatttg cgatccccca agagttagag aaaagtgtac agatgtttga
     1801 attgttttat agccagcatt tcagtggaag gaaactgaca tggttacatt atctctgtac
     1861 aggtgaagtt aaaatgaact atttggggaa accatatgta gctatggtta caacctacca
     1921 aatggcagtt cttcttgcct ttaacaacag tgaaactgtc agctacaaag agcttcaaga
     1981 cagcacccag atgaatgaaa aggagctaac taaaacaatc aaatcattac ttgatgtgaa
     2041 aatgattaac catgattcag aaaaggaaga tatcgatgca gaatcttcat tttcactaaa
     2101 tatgagcttc agcagtaaga gaacaaaatt taagattact acatcaatgc agaaggatac
     2161 accacaagaa ctggaacaga ctagaagtgc tgtagatgag gaccggaaaa tgtatcttca
     2221 agctgctatt gtccgcatca tgaaagcacg gaaagtgctt cggcacaacg ccctcattca
     2281 ggaggtaatc agccagtcaa gagccaggtt caatcccagt atcagcatga ttaagaagtg
     2341 catagaagtt ctgatagaca agcagtacat cgagcgcagc caagcatctg cagatgaata
     2401 cagttacgtt gcgtgacgcc ttctcctggt gcgggtgtga ggtcattgcc atccccattt
     2461 ggtgagctcc tgtgggagaa agcaggcctg tgcctccata ctttggtcgt ctggcagccc
     2521 ctgtttttct gctgtttaca acatcaccag tgccacgtca tgagcgtcag agaaaatgcc
     2581 tagagatatt caagctcatg tcattatgac gtttcttaaa actttattaa aagaatgagt
     2641 gaagtattgc tgagatgtgg aattcggctg ggtaccatgc tttctcccct tcacgtttgc
     2701 agttgatgtg tttttttttt ttttttaaat gtatcttaaa ggacataaaa taaaaactta
     2761 aatattgtaa tatgacagat aacctaataa ttgtatctac attaaaatga caaacatgat
     2821 gttgctgctt gtcaaataaa aaaaaataaa cataacgcct tttttctatg ttgatggaat
     2881 atcaggttgg acagaaaata agacagttgt aaatgcccac ccaagcagca aacagcgttc
     2941 cttcattcat cttggtgatg aattcacttg ggcccccatc tattcttcaa ctaaaactgc
     3001 cagggttctc tttttaaata cataagattg tgaatttgag ctaatggtgt agtaagcagg
     3061 ttttgtattt tgaggaggat gtgtctggtg gtggtgtggt tacaacagaa atgagtagta
     3121 gctactgtgc agggacttgt ggaccagcag aaaagaactg ctcactcttg acctattcat
     3181 gatgcatgga tactaacata atggctgtgg ctcgtttccc agagtttagg aggctgatta
     3241 caatgagttc cttctagctc tccctgccca tcactggcac ccttccccag tgcagatgac
     3301 tcctccatga caagttgata acagtgcccc cataacacta ggccattcca ccaggcacct
     3361 gtatatggac atgagatttc atgagtttgt ttcttgtaga caacaggggc attcaaatac
     3421 tctgggtacc gtattctcca ccatctctgt atcacatcgc tagagtattt acatcttaac
     3481 agataaggcc ttaaacccac agtaatattc cgaacaaata aacagcactt cattttcatt
     3541 ctcatgtggt tcatatttaa atttatttaa tgattggttg atttgattgc aaatcttttg
     3601 tatctcaagt gttcaaaaat ctcctggact tctaatggtt tccgctgtct ttatgtatgg
     3661 ttttgccttc ctacctcgtg tggataaggg cagagcatat actattgtac attcgtggtc
     3721 aaagaacaag gcctttgttt tgataacttg ttgataagtt ttccaaattt ataaatatta
     3781 aattaagtcg taacctga
//
