LOCUS       NP_001032171             528 aa            linear   ROD 10-FEB-2025
DEFINITION  cytoplasmic tRNA 2-thiolation protein 2 [Rattus norvegicus].
ACCESSION   NP_001032171 XP_214702
VERSION     NP_001032171.1
DBSOURCE    REFSEQ: accession NM_001037094.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 528)
  AUTHORS   Schlieker,C.D., Van der Veen,A.G., Damon,J.R., Spooner,E. and
            Ploegh,H.L.
  TITLE     A functional proteomics approach links the ubiquitin-related
            modifier Urm1 to a tRNA modification pathway
  JOURNAL   Proc Natl Acad Sci U S A 105 (47), 18255-18260 (2008)
   PUBMED   19017811
  REMARK    GeneRIF: Identifies a similar human protein that is involved in
            tRNA modification.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC107464.1.
            
            On Nov 7, 2005 this sequence version replaced XP_214702.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC107464.1, BC079393.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132263, SAMD00132265
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     Protein         1..528
                     /product="cytoplasmic tRNA 2-thiolation protein 2"
                     /note="cytosolic thiouridylase subunit 2 homolog"
                     /calculated_mol_wt=57610
     Region          1..23
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q3B7U4.2)"
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylcysteine.
                     /evidence=ECO:0000250|UniProtKB:Q2VPK5; propagated from
                     UniProtKB/Swiss-Prot (Q3B7U4.2)"
     Region          196..219
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q3B7U4.2)"
     Region          <254..375
                     /region_name="TilS"
                     /note="tRNA(Ile)-lysidine synthase TilS/MesJ [Translation,
                     ribosomal structure and biogenesis]; COG0037"
                     /db_xref="CDD:439807"
     Region          342..465
                     /region_name="CTU2"
                     /note="Cytoplasmic tRNA 2-thiolation protein 2; pfam10288"
                     /db_xref="CDD:463044"
     Site            416
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q2VPK5; propagated from
                     UniProtKB/Swiss-Prot (Q3B7U4.2)"
     Region          504..528
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q3B7U4.2)"
     CDS             1..528
                     /gene="Ctu2"
                     /gene_synonym="Ncs2"
                     /coded_by="NM_001037094.1:13..1599"
                     /db_xref="GeneID:292069"
                     /db_xref="RGD:1562594"
ORIGIN      
        1 mcqagedyag paqrepppvp rpsreqkcvk caeglpvvvi ragdafcrdc fkafyvhkfr
       61 amlgknrvif pgekvllsws ggpssssmvw qvleglsqds akrlrfvpgv iyvdegaacg
      121 qsledrvktl aevkrilgnt gfpfhvvale evfslppsvl rrasqepagt eeaykaavds
      181 flqqqhvlgt eacaspaqgq gqlrpshsqe psgtvghpkd aqtealsrlf ksiktltake
      241 ellqtlrthl tvhvarshgy rkvmtgesct rlaiklmtnl algrgaflaw dtgfsderhg
      301 dvvlvrpmrd htlkevafyn rlfgvpsvft paidtkapek asihrlmeaf ilklqtlfps
      361 tvstvyrtse klvkapregc atgpsgpncl lcmcaldvdn adsatafgaq ssshlsqmlt
      421 aeagtptrpc cgagegqtqs chravgrred awaciieqlc yscrvnmkdl psldplppyv
      481 lteaqlrsqr gsvseeiqey lieeeeeedr aepceamkqe aedkgigl
//
