LOCUS       NM_012536                874 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus chymotrypsinogen B1 (Ctrb1), mRNA.
ACCESSION   NM_012536 XM_001077691
VERSION     NM_012536.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Miao,Q., Sun,Y., Wei,T., Zhao,X., Zhao,K., Yan,L., Zhang,X., Shu,H.
            and Yang,F.
  TITLE     Chymotrypsin B cached in rat liver lysosomes and involved in
            apoptotic regulation through a mitochondrial pathway
  JOURNAL   J Biol Chem 283 (13), 8218-8228 (2008)
   PUBMED   18211899
  REMARK    GeneRIF: chymotrypsin B is not only restricted to the pancreas but
            can function intracellularly as a pro-apoptotic protease
REFERENCE   2  (bases 1 to 874)
  AUTHORS   Kwitek,A.E., Gullings-Handley,J., Yu,J., Carlos,D.C., Orlebeke,K.,
            Nie,J., Eckert,J., Lemke,A., Andrae,J.W., Bromberg,S., Pasko,D.,
            Chen,D., Scheetz,T.E., Casavant,T.L., Soares,M.B., Sheffield,V.C.,
            Tonellato,P.J. and Jacob,H.J.
  TITLE     High-density rat radiation hybrid maps containing over 24,000
            SSLPs, genes, and ESTs provide a direct link to the rat genome
            sequence
  JOURNAL   Genome Res 14 (4), 750-757 (2004)
   PUBMED   15060019
REFERENCE   3  (bases 1 to 874)
  AUTHORS   Bodi,A., Kaslik,G., Venekei,I. and Graf,L.
  TITLE     Structural determinants of the half-life and cleavage site
            preference in the autolytic inactivation of chymotrypsin
  JOURNAL   Eur J Biochem 268 (23), 6238-6246 (2001)
   PUBMED   11733020
REFERENCE   4  (bases 1 to 874)
  AUTHORS   Venekei,I., Szilagyi,L., Graf,L. and Rutter,W.J.
  TITLE     Attempts to convert chymotrypsin to trypsin
  JOURNAL   FEBS Lett 379 (2), 143-147 (1996)
   PUBMED   8635580
REFERENCE   5  (bases 1 to 874)
  AUTHORS   Oppezzo,O., Ventura,S., Bergman,T., Vendrell,J., Jornvall,H. and
            Aviles,F.X.
  TITLE     Procarboxypeptidase in rat pancreas. Overall characterization and
            comparison of the activation processes
  JOURNAL   Eur J Biochem 222 (1), 55-63 (1994)
   PUBMED   8200353
REFERENCE   6  (bases 1 to 874)
  AUTHORS   von Deimling,O.H., Groning,J. and Gaa,A.
  TITLE     Genetic and biochemical characterization of a new chymotrypsin
            isozyme of the house mouse, CTRA-1
  JOURNAL   Biochem Genet 29 (11-12), 545-557 (1991)
   PUBMED   1820020
REFERENCE   7  (bases 1 to 874)
  AUTHORS   Iovanna,J.L., Keim,V., Michel,R. and Dagorn,J.C.
  TITLE     Pancreatic gene expression is altered during acute experimental
            pancreatitis in the rat
  JOURNAL   Am J Physiol 261 (3 Pt 1), G485-G489 (1991)
   PUBMED   1716058
REFERENCE   8  (bases 1 to 874)
  AUTHORS   Iovanna,J.L., Dusetti,N.J., Cadenas,M.B., Mallmann,M.C. and
            Calvo,E.L.
  TITLE     Changes in pancreatic trophism and gene expression during a
            prolonged fasting period in rats
  JOURNAL   Int J Pancreatol 8 (2), 177-186 (1991)
   PUBMED   1709673
REFERENCE   9  (bases 1 to 874)
  AUTHORS   Rosewicz,S., Lewis,L.D., Wang,X.Y., Liddle,R.A. and Logsdon,C.D.
  TITLE     Pancreatic digestive enzyme gene expression: effects of CCK and
            soybean trypsin inhibitor
  JOURNAL   Am J Physiol 256 (4 Pt 1), G733-G738 (1989)
   PUBMED   2468294
REFERENCE   10 (bases 1 to 874)
  AUTHORS   Lau,P.P., Palmer,R.L., Lambert,H.C., Song,C.S., Lutz,F. and
            Geokas,M.C.
  TITLE     Pseudomonas aeruginosa cytotoxin stimulates secretion of amylase
            and protease zymogens with a concomitant decrease of mRNA levels in
            isolated rat pancreatic acini
  JOURNAL   Biochem Biophys Res Commun 152 (2), 688-694 (1988)
   PUBMED   2452633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Sep 22, 2022 this sequence version replaced NM_012536.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ234339.1, FQ233595.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389, SAMN12840132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                JAXUCZ010000019.1  56566904-56566978   c
            76-179              JAXUCZ010000019.1  56564601-56564704   c
            180-259             JAXUCZ010000019.1  56564433-56564512   c
            260-338             JAXUCZ010000019.1  56564250-56564328   c
            339-519             JAXUCZ010000019.1  56563834-56564014   c
            520-653             JAXUCZ010000019.1  56562796-56562929   c
            654-874             JAXUCZ010000019.1  56562220-56562440   c
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..874
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /note="chymotrypsinogen B1"
                     /db_xref="GeneID:24291"
                     /db_xref="RGD:2444"
     exon            1..75
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..815
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /EC_number="3.4.21.1"
                     /note="Chymotrypsin B; Chymotrypsinogen B; chymotrypsin
                     B1"
                     /codon_start=1
                     /product="chymotrypsinogen B precursor"
                     /protein_id="NP_036668.2"
                     /db_xref="GeneID:24291"
                     /db_xref="RGD:2444"
                     /translation="MAFLWLLSCFALVGATFGCGVPTIQPVLTGLSRIVNGEDAIPGS
                     WPWQVSLQDKTGFHFCGGSLISEDWVVTAAHCGVKTSDVVVAGEFDQGSDEENIQVLK
                     IAQVFKNPKFNMFTVRNDITLLKLATPAQFSETVSAVCLPNVDDDFPPGTVCATTGWG
                     KTKYNALKTPEKLQQAALPIVSEADCKKSWGSKITDVMICAGASGVSSCMGDSGGPLV
                     CQKDGVWTLAGIVSWGSGVCSTSTPAVYSRVTALMPWVQQILEAN"
     exon            76..179
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /inference="alignment:Splign:2.1.0"
     exon            180..259
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /inference="alignment:Splign:2.1.0"
     exon            260..338
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /inference="alignment:Splign:2.1.0"
     exon            339..519
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /inference="alignment:Splign:2.1.0"
     exon            520..653
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /inference="alignment:Splign:2.1.0"
     exon            654..874
                     /gene="Ctrb1"
                     /gene_synonym="Ctrb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cactcactcc cttgaccagc accatggcat tcctttggct cctgtcctgc tttgcccttg
       61 tgggggccac ctttggctgt ggagtcccta ccatccaacc cgtgctgact ggcctgtcca
      121 ggatcgtcaa cggagaggat gctattcccg gctcctggcc ctggcaggtg tctctgcagg
      181 ataaaactgg cttccatttc tgcgggggct ccctcatcag cgaggactgg gtggtcactg
      241 ctgcccactg cggggtcaag acctccgacg tggtggtagc tggagagttt gaccagggct
      301 ccgacgaaga gaacatccag gtcctgaaga tcgcacaggt ctttaagaac cccaagttca
      361 atatgttcac tgtgcgcaat gacatcactc tgctgaagct ggccactcct gcccagttct
      421 ctgagactgt gtctgccgtg tgtctaccca acgtcgatga tgacttccct cctggcactg
      481 tgtgcgccac cactggctgg ggcaagacca aatacaatgc cctcaagacc cctgagaagc
      541 tgcagcaggc cgccctgccc atcgtgtccg aggctgactg caagaagtcc tggggttcca
      601 agatcaccga tgtgatgatc tgcgcaggcg ctagcggcgt ctcttcctgc atgggtgact
      661 ccggtggccc cctcgtctgc cagaaagatg gagtctggac cctggcaggc attgtgtcct
      721 ggggcagtgg cgtctgttcc acttccactc ctgctgtgta ttcccgagtc acagccctca
      781 tgccctgggt tcagcagatc ttggaagcca actgagtccg tggttcttgc caacccccct
      841 ctgagcatct acaataaaaa tcatctaatt agaa
//
