LOCUS       NM_001034998            3272 bp    mRNA    linear   ROD 20-SEP-2024
DEFINITION  Rattus norvegicus CTP synthase 2 (Ctps2), mRNA.
ACCESSION   NM_001034998
VERSION     NM_001034998.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Lynch,E.M. and Kollman,J.M.
  TITLE     Coupled structural transitions enable highly cooperative regulation
            of human CTPS2 filaments
  JOURNAL   Nat Struct Mol Biol 27 (1), 42-48 (2020)
   PUBMED   31873303
REFERENCE   3  (bases 1 to 3272)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3272)
  AUTHORS   Tan,W.L., Bhattacharya,B., Loh,M., Balasubramanian,I., Akram,M.,
            Dong,D., Wong,L., Thakkar,B., Salto-Tellez,M., Soo,R.A.,
            Fichtner,I., Iacopetta,B. and Soong,R.
  TITLE     Low cytosine triphosphate synthase 2 expression renders resistance
            to 5-fluorouracil in colorectal cancer
  JOURNAL   Cancer Biol Ther 11 (6), 599-608 (2011)
   PUBMED   21378502
REFERENCE   5  (bases 1 to 3272)
  AUTHORS   Kassel,K.M., Au da,R., Higgins,M.J., Hines,M. and Graves,L.M.
  TITLE     Regulation of human cytidine triphosphate synthetase 2 by
            phosphorylation
  JOURNAL   J Biol Chem 285 (44), 33727-33736 (2010)
   PUBMED   20739275
REFERENCE   6  (bases 1 to 3272)
  AUTHORS   van Kuilenburg,A.B., Meinsma,R., Vreken,P., Waterham,H.R. and van
            Gennip,A.H.
  TITLE     Identification of a cDNA encoding an isoform of human CTP
            synthetase
  JOURNAL   Biochim Biophys Acta 1492 (2-3), 548-552 (2000)
   PUBMED   10899599
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC085949.1, DV724473.1 and JAXUCZ010000021.1.
            
            On Sep 11, 2012 this sequence version replaced NM_001034998.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085949.1, SRR26643293.32492.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation, longest
                                                protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2099              BC085949.1         1-2099
            2100-2684           DV724473.1         62-646
            2685-3272           JAXUCZ010000021.1  35277658-35278245   c
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq14"
     gene            1..3272
                     /gene="Ctps2"
                     /note="CTP synthase 2"
                     /db_xref="GeneID:619580"
                     /db_xref="RGD:1563369"
     exon            1..51
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            52..256
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1851
                     /gene="Ctps2"
                     /EC_number="6.3.4.2"
                     /note="CTP synthetase 2; UTP--ammonia ligase 2; cytidine
                     5'-triphosphate synthase 2; cytidine triphosphate synthase
                     II; CTP synthase II"
                     /codon_start=1
                     /product="CTP synthase 2"
                     /protein_id="NP_001030170.1"
                     /db_xref="GeneID:619580"
                     /db_xref="RGD:1563369"
                     /translation="MKYILVTGGVISGIGKGIIASSIGTILKSCGLRVTAIKIDPYIN
                     IDAGTFSPYEHGEVFVLNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINKER
                     RGDYLGKTVQVVPHITDAIQDWVMNQAKVSVDGNKEDPQICVIELGGTIGDIEGMAFV
                     EAFRQFQFKAKRENFYNIHVSLVPQPSATGEQKTKPTQNSVRALRGLGLSPDLIVCRS
                     STPIEMAVKEKISMFCHVNPEQVICIHDVSSIYRVPLLLEEQGVVKYFQERLDLPIND
                     CSNNLLFKWKTMADRYERLQKICSIALVGKYTKLRDCYASVFKALEHSALAINHKLNL
                     MYIDSIDLEPVTKAEDPVKFHEAWQKLCLADGILVPGGFGIRGTLGKLQAISWARTKK
                     IPFLGICLGMQLAVIEFARNCLNLKDANSTEFDPNTPVPLVIDMPEHNPGDLGGTMRL
                     GLRRTVFTTENSILKKLYGDVPYIEERHRHRYEVNPNLINQFENKDLCFVGEDVDGKR
                     MEIIELTGHPYFIGVQFHPEFSSRPMKPSPPYLGLLLAATGTLNTHLQQMSKLSYSDI
                     YSDASDDSFSEAKFAELDIN"
     exon            257..427
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            428..528
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            529..645
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            646..729
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            730..810
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            811..962
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1095
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1184
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1279
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1342
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1386
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1483
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1539
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1636
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1781
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1893
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..3272
                     /gene="Ctps2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctgtatctc cctctcttcc gcagatggcc cagaagggaa aaaagacaat aattgcatct
       61 gggtattggg agtcacccat ttgcccgcca atgaagtata tcctggttac tggtggggtc
      121 atctcaggca ttggtaaagg gatcatcgcc agcagcattg ggaccattct caagtcatgt
      181 ggactccgag taaccgccat caagatcgac ccctacatta acattgatgc tggcactttc
      241 tcaccttacg aacatggtga agtatttgtt ttaaatgatg gtggagaagt tgatttggac
      301 cttggaaatt atgaaagatt cttggacatt aatctttata aagacaacaa catcaccact
      361 gggaagatat atcagcatgt gatcaacaaa gagaggcgcg gtgattatct ggggaaaact
      421 gtccaagtgg tcccccacat tactgatgct atccaagact gggttatgaa tcaagccaag
      481 gtgtctgtgg atggcaataa ggaagatccc cagatatgtg ttatcgagct gggaggcacc
      541 attggagaca tcgaagggat ggcatttgtg gaagcattcc gacaattcca gtttaaagca
      601 aagagagaga atttctataa tattcatgtc agccttgtgc cacagcccag tgctactgga
      661 gaacaaaaaa ccaagcctac acaaaacagt gtccgtgcgc tgagagggct gggcttgtct
      721 ccggatctga ttgtctgccg aagttcaaca cccattgaga tggctgtgaa ggagaagatt
      781 tctatgtttt gtcacgtgaa ccctgaacag gtcatctgta tccatgatgt ttcttccatc
      841 taccgagtcc ctcttctttt ggaggagcaa ggtgtggtta aatattttca agagagattg
      901 gacctgccca tcaatgattg ctcaaataat ttgcttttca agtggaaaac catggctgac
      961 aggtatgaaa gattgcagaa aatatgttcc atagccctgg ttggtaaata caccaaactc
     1021 agggactgct acgcctctgt gttcaaagcc ctggaacact cagccttggc catcaaccac
     1081 aagttgaatc tgatgtacat agactccatt gatctggagc cagtcaccaa agctgaggat
     1141 cctgtgaaat tccatgaagc ttggcagaag ctgtgcttag ctgatggtat tctcgtgcct
     1201 ggaggctttg gaatccgagg aacattggga aaactccagg caatttcttg ggcaaggaca
     1261 aagaagattc catttctggg aatttgcctt gggatgcaac tggcagtgat tgagtttgca
     1321 agaaactgcc tgaacttgaa agatgctaat tctacagaat ttgatccaaa cactccagtc
     1381 ccattggtaa ttgacatgcc tgaacacaac cctggcgatt tgggaggaac aatgagactg
     1441 ggattaagac gaactgtttt cacaactgaa aattccatac tgaagaagct ttacggtgat
     1501 gttccttaca tagaagaaag acaccgacat cggtatgagg tgaaccctaa cctgatcaac
     1561 caatttgaga ataaagacct ttgctttgtc ggtgaggatg tggatgggaa gaggatggag
     1621 attattgaat tgacaggtca tccatatttc atcggcgtcc aattccatcc tgagttttcc
     1681 tctaggccaa tgaagccttc ccctccgtac ctgggactct tacttgcagc aaccggaact
     1741 ctgaataccc atctgcaaca gatgagcaaa ctatcgtaca gtgacatata cagtgatgcc
     1801 agtgatgata gcttttcaga ggcgaagttt gctgagctgg atatcaactg aagctctttc
     1861 ttgcctcagg aatggcagaa gccacctaag aggcctctga agtaattata cttaagatga
     1921 aagaattatc tgaagaaacc accactctgt tccctagaca tgggctgtga agattcaaag
     1981 ggaatctgca aatgtttact tcatgaaaca gaaccaaagg gattcttttc ttttccctcc
     2041 agaggcagcc tgtagtattc acacatttct gtacttaatt aaatgtaatt caacagcaat
     2101 cccggggtag aaagtgtttt tgttttgtcc agaacatggt gagttcaagg tggtaagcag
     2161 gaattggggg aactttgtct tcacagtatt gcatgggtaa ggtggtgggc atatgctata
     2221 accaattttg ggtttaaaaa tgagttgttc tcttacctgg acatgaacta caggttagta
     2281 tgggagttaa gattatttta cgttctcatt ctgtttattt ccagtaagtc tattttctca
     2341 aatataaaat gtaaaaggga atctgaaaac ttaggaatcg gggtaagtag ggctgagttc
     2401 catctcttca acccacacag agcagcaaac cagtacccac aggtcttctg accccagcat
     2461 gtgcacacac agacacaccc actaaaaatc attaaaaaca aaacaataat cactttgttt
     2521 ttttaaattc aaattacttt ttattattct tttgacaact tcgtacatac atagaattat
     2581 tttggtcatt taaaaacttt tttcagagtg atttatttat ttattatttt tcatatccca
     2641 gttgttgccc cctcttttta ttacactctc actccattct cactgaaacc cttcttctca
     2701 aataagtccc tcttttattt tcatgtcttt tctgttgctt tgtctttttc ttattgagat
     2761 agcatctcac tccgtagtgc aagctaacat gggacttact gtgtagtcgg ctatagtctt
     2821 ggctcactgt gatcctctgg cctcagcctc tcatgagtta ggattatagg tgtgagctgt
     2881 tcttctgtgc tcaagatcag catttttgtt tggtttttga gacaagtttt tgctacgtag
     2941 ccctggttgt actggaactc actgtggctg gccttaaagt cacaaagata tgccattctc
     3001 tgcctctcga gtgctggagt taaaggcata tacaaacaca cttggctaac ttttttttca
     3061 gttggaaaaa taacaattgt aaagtgaatt atgcattggc atttactgat acttcacagc
     3121 tctcagtttt atgaaattac tccagaatta acctctgttc ccatgggcag tcactccaga
     3181 ggtatttctt tctgtcctca tcctctggca actcttgtaa ctacattgat caaggcaact
     3241 tggaaataaa agaaagcact gatcaacctc ta
//
