LOCUS       NM_012838                610 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus cystatin B (Cstb), mRNA.
ACCESSION   NM_012838
VERSION     NM_012838.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Kubo,A., Shirato,I., Hidaka,T., Takagi,M., Sasaki,Y., Asanuma,K.,
            Ishidoh,K. and Suzuki,Y.
  TITLE     Expression of Cathepsin L and Its Intrinsic Inhibitors in Glomeruli
            of Rats With Puromycin Aminonucleoside Nephrosis
  JOURNAL   J Histochem Cytochem 66 (12), 863-877 (2018)
   PUBMED   30052474
  REMARK    GeneRIF: In contrast to the weak levels of cathepsin L in normal
            glomeruli, on days 4 and 8, strong immunostaining was detected in
            almost all podocytes when proteinuria and pathological changes of
            the podocytes developed. Cathepsin L was reduced after day 28, but
            remained in a focal and segmental manner. Cystatin B, an
            intracellular inhibitor, was not detected in podocytes.
REFERENCE   2  (bases 1 to 610)
  AUTHORS   Ulbrich,L., Cozzolino,M., Marini,E.S., Amori,I., De Jaco,A.,
            Carri,M.T. and Augusti-Tocco,G.
  TITLE     Cystatin B and SOD1: protein-protein interaction and possible
            relation to neurodegeneration
  JOURNAL   Cell Mol Neurobiol 34 (2), 205-213 (2014)
   PUBMED   24234043
  REMARK    GeneRIF: A reciprocal influence of CSTB and SOD1 at the gene
            expression level and for a direct interaction of the two proteins,
            is reported.
REFERENCE   3  (bases 1 to 610)
  AUTHORS   Rispoli,A., Cipollini,E., Catania,S., Di Giaimo,R., Pulice,G., van
            Houte,S., Sparla,F., Dal Piaz,F., Roncarati,D., Trost,P. and
            Melli,M.
  TITLE     Insights in progressive myoclonus epilepsy: HSP70 promotes cystatin
            B polymerization
  JOURNAL   Biochim Biophys Acta 1834 (12), 2591-2599 (2013)
   PUBMED   24063889
  REMARK    GeneRIF: Cu(2+) modifies the redox environment of the protein,
            allowing the oxidation of the cysteine residue of CSTB that
            triggers polymerization.
REFERENCE   4  (bases 1 to 610)
  AUTHORS   Pieragostino,D., Agnifili,L., Fasanella,V., D'Aguanno,S.,
            Mastropasqua,R., Di Ilio,C., Sacchetta,P., Urbani,A. and Del
            Boccio,P.
  TITLE     Shotgun proteomics reveals specific modulated protein patterns in
            tears of patients with primary open angle glaucoma naive to therapy
  JOURNAL   Mol Biosyst 9 (6), 1108-1116 (2013)
   PUBMED   23580065
REFERENCE   5  (bases 1 to 610)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 610)
  AUTHORS   Sato,N., Ishidoh,K., Uchiyama,Y. and Kominami,E.
  TITLE     Structural organization of the gene encoding rat cystatin beta
  JOURNAL   Gene 114 (2), 257-260 (1992)
   PUBMED   1601307
REFERENCE   7  (bases 1 to 610)
  AUTHORS   Sato,N., Ishidoh,K., Uchiyama,Y. and Kominami,E.
  TITLE     Molecular cloning and sequencing of cDNA for rat cystatin beta
  JOURNAL   Nucleic Acids Res 18 (22), 6698 (1990)
   PUBMED   2251135
REFERENCE   8  (bases 1 to 610)
  AUTHORS   Jerala,R., Trstenjak,M., Lenarcic,B. and Turk,V.
  TITLE     Cloning a synthetic gene for human stefin B and its expression in
            E. coli
  JOURNAL   FEBS Lett 239 (1), 41-44 (1988)
   PUBMED   3053245
REFERENCE   9  (bases 1 to 610)
  AUTHORS   Abrahamson,M., Barrett,A.J., Salvesen,G. and Grubb,A.
  TITLE     Isolation of six cysteine proteinase inhibitors from human urine.
            Their physicochemical and enzyme kinetic properties and
            concentrations in biological fluids
  JOURNAL   J Biol Chem 261 (24), 11282-11289 (1986)
   PUBMED   3488317
REFERENCE   10 (bases 1 to 610)
  AUTHORS   Fukuda,I., Yoshida,T., Yoritsune,H., Kiso,A. and Kamide,H.
  TITLE     [Physical training aspect of exercise testing: the evaluation of
            Master's double 2 step test, augmented Master's test and Cooper's
            submaximum single-level load exercise test (author's transl)]
  JOURNAL   Kokyu To Junkan 23 (11), 985-992 (1975)
   PUBMED   1239805
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF976177.1 and BC084725.1.
            
            On Oct 20, 2007 this sequence version replaced NM_012838.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC084725.1, CO402879.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-345               CF976177.1         2-346
            346-610             BC084725.1         334-598
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..610
                     /gene="Cstb"
                     /gene_synonym="Cyb; Epm1; Stfb"
                     /note="cystatin B"
                     /db_xref="GeneID:25308"
                     /db_xref="RGD:2435"
     exon            1..163
                     /gene="Cstb"
                     /gene_synonym="Cyb; Epm1; Stfb"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..394
                     /gene="Cstb"
                     /gene_synonym="Cyb; Epm1; Stfb"
                     /note="cystatin beta; stefin-B; liver thiol proteinase
                     inhibitor; cystatin B (stefin B)"
                     /codon_start=1
                     /product="cystatin-B"
                     /protein_id="NP_036970.1"
                     /db_xref="GeneID:25308"
                     /db_xref="RGD:2435"
                     /translation="MMCGAPSATMPATTETQEIADKVKSQLEEKANQKFDVFKAISFR
                     RQVVAGTNFFIKVDVGEEKCVHLRVFEPLPHENKPLTLSSYQTDKEKHDELTYF"
     exon            164..265
                     /gene="Cstb"
                     /gene_synonym="Cyb; Epm1; Stfb"
                     /inference="alignment:Splign:2.1.0"
     exon            266..581
                     /gene="Cstb"
                     /gene_synonym="Cyb; Epm1; Stfb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggggccgagc caggtttttc tagggtccag acacccaggt ctcctagttg gctctctccg
       61 tagcttctct gtgatattct aaccagtgct tgccaagatg atgtgtggcg cgccatccgc
      121 cacaatgccg gccacgaccg agacgcagga gatcgccgac aaggtgaagt ctcaacttga
      181 agagaaagca aatcagaagt ttgatgtctt taaagccata tccttcagga gacaggtagt
      241 ggccggcacc aacttcttca tcaaggttga tgtcggcgaa gaaaaatgtg tgcacttgag
      301 ggtgtttgaa cccctccctc atgagaacaa gcctttgacc ttgtcttctt accagaccga
      361 caaagaaaag cacgatgagc taacctactt ctgattctgc agcccctttg ccaaatactt
      421 cacctttgga atccgtgttt gggaccacga agtaaatacc cctctgtgag cagcttcctt
      481 tgtgatgccc aaacggcgtt gtattttgtt tctttccaaa caattatttt cagaaaactg
      541 tataaaacta tctctctaaa tatatatttt tagagaccgt aaaaaaaaaa aaaaaaaaaa
      601 aaaaaaaaaa
//
