LOCUS       NM_012935               1019 bp    mRNA    linear   ROD 23-APR-2024
DEFINITION  Rattus norvegicus crystallin, alpha B (Cryab), mRNA.
ACCESSION   NM_012935
VERSION     NM_012935.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Bartelt-Kirbach,B. and Golenhofen,N.
  TITLE     Regulation of rat HspB5/alphaB-Crystallin by microRNAs miR-101a-3p,
            miR-140-5p, miR-330-5p, and miR-376b-3p
  JOURNAL   Cell Stress Chaperones 28 (6), 787-799 (2023)
   PUBMED   37584866
  REMARK    GeneRIF: Regulation of rat HspB5/alphaB-Crystallin by microRNAs
            miR-101a-3p, miR-140-5p, miR-330-5p, and miR-376b-3p.
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Bartelt-Kirbach,B., Wiegreffe,C., Birk,S., Baur,T., Moron,M.,
            Britsch,S. and Golenhofen,N.
  TITLE     HspB5/alphaB-crystallin phosphorylation at S45 and S59 is essential
            for protection of the dendritic tree of rat hippocampal neurons
  JOURNAL   J Neurochem 157 (6), 2055-2069 (2021)
   PUBMED   33220080
  REMARK    GeneRIF: HspB5/alphaB-crystallin phosphorylation at S45 and S59 is
            essential for protection of the dendritic tree of rat hippocampal
            neurons.
REFERENCE   3  (bases 1 to 1019)
  AUTHORS   Wang,L., Nie,Q., Gao,M., Yang,L., Xiang,J.W., Xiao,Y., Liu,F.Y.,
            Gong,X.D., Fu,J.L., Wang,Y., Nguyen,Q.D., Liu,Y., Liu,M. and
            Li,D.W.
  TITLE     The transcription factor CREB acts as an important regulator
            mediating oxidative stress-induced apoptosis by suppressing
            alphaB-crystallin expression
  JOURNAL   Aging (Albany NY) 12 (13), 13594-13617 (2020)
   PUBMED   32554860
  REMARK    GeneRIF: The transcription factor CREB acts as an important
            regulator mediating oxidative stress-induced apoptosis by
            suppressing alphaB-crystallin expression.
REFERENCE   4  (bases 1 to 1019)
  AUTHORS   Hang,L.H., Chen,H.M., Yu,J.M., Xu,Y. and Li,S.N.
  TITLE     Evidence of the involvement of spinal alphaB-crystallin in the
            maintenance of bone cancer pain in rats
  JOURNAL   Pharmacol Rep 72 (1), 208-213 (2020)
   PUBMED   32016842
  REMARK    GeneRIF: Evidence of the involvement of spinal alphaB-crystallin in
            the maintenance of bone cancer pain in rats.
REFERENCE   5  (bases 1 to 1019)
  AUTHORS   Roquemore,E.P., Chevrier,M.R., Cotter,R.J. and Hart,G.W.
  TITLE     Dynamic O-GlcNAcylation of the small heat shock protein alpha
            B-crystallin
  JOURNAL   Biochemistry 35 (11), 3578-3586 (1996)
   PUBMED   8639509
  REMARK    GeneRIF: Alpha crystallin is a moonlighting protein that functions
            as a heat shock protein as well as a lens crystallin.
REFERENCE   6  (bases 1 to 1019)
  AUTHORS   Atomi,Y., Yamada,S. and Nishida,T.
  TITLE     Early changes of alpha B-crystallin mRNA in rat skeletal muscle to
            mechanical tension and denervation
  JOURNAL   Biochem Biophys Res Commun 181 (3), 1323-1330 (1991)
   PUBMED   1764082
REFERENCE   7  (bases 1 to 1019)
  AUTHORS   Bhat,S.P., Horwitz,J., Srinivasan,A. and Ding,L.
  TITLE     Alpha B-crystallin exists as an independent protein in the heart
            and in the lens
  JOURNAL   Eur J Biochem 202 (3), 775-781 (1991)
   PUBMED   1765091
REFERENCE   8  (bases 1 to 1019)
  AUTHORS   Atomi,Y., Yamada,S., Strohman,R. and Nonomura,Y.
  TITLE     Alpha B-crystallin in skeletal muscle: purification and
            localization
  JOURNAL   J Biochem 110 (5), 812-822 (1991)
   PUBMED   1783614
REFERENCE   9  (bases 1 to 1019)
  AUTHORS   Iwaki,A., Iwaki,T., Goldman,J.E. and Liem,R.K.
  TITLE     Multiple mRNAs of rat brain alpha-crystallin B chain result from
            alternative transcriptional initiation
  JOURNAL   J Biol Chem 265 (36), 22197-22203 (1990)
   PUBMED   2176207
REFERENCE   10 (bases 1 to 1019)
  AUTHORS   Bhat,S.P. and Nagineni,C.N.
  TITLE     alpha B subunit of lens-specific protein alpha-crystallin is
            present in other ocular and non-ocular tissues
  JOURNAL   Biochem Biophys Res Commun 158 (1), 319-325 (1989)
   PUBMED   2912453
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M55534.1 and CK357656.1.
            
            On Sep 16, 2014 this sequence version replaced NM_012935.3.
            
            Summary: This gene encodes subunit b, one of two subunits of
            alpha-crystallin, which is a high molecular weight, soluble
            aggregate and is a member of the small heat shock protein family.
            The encoded protein has been identified as a moonlighting protein
            based on its ability to perform mechanistically distinct functions.
            It acts as a molecular chaperone and is the major protein in the
            eye lens, maintaining the transparency and refractive index of the
            lens. Alternate promoter usage results in different transcript
            variants encoding the same protein. [provided by RefSeq, Sep 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ228589.1, M55534.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s) :: PMID: 8639509
            RefSeq Select criteria          :: based on conservation,
                                               expression, longest protein
            regulatory uORF                 :: PMID: 2176207
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-994               M55534.1           254-1247
            995-1019            CK357656.1         679-703
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q23"
     gene            1..1019
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
                     /note="crystallin, alpha B"
                     /db_xref="GeneID:25420"
                     /db_xref="RGD:2414"
     exon            1..530
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      30..86
                     /regulatory_class="uORF"
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:2176207]"
                     /note="this uORF is predicted to encode a 18 aa peptide
                     (PMID:2176207)"
     regulatory      151..324
                     /regulatory_class="uORF"
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:2176207]"
                     /note="this uORF is predicted to encode a 57 aa peptide
                     (PMID:2176207)"
     regulatory      188..277
                     /regulatory_class="uORF"
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:2176207]"
                     /note="this uORF is predicted to encode a 29 aa peptide
                     (PMID:2176207)"
     CDS             330..857
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
                     /note="alpha(B)-crystallin; Crystallin, alpha polypeptide
                     2"
                     /codon_start=1
                     /product="alpha-crystallin B chain"
                     /protein_id="NP_037067.1"
                     /db_xref="GeneID:25420"
                     /db_xref="RGD:2414"
                     /translation="MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFSTATSL
                     SPFYLRPPSFLRAPSWIDTGLSEMRMEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHG
                     KHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQASGPERTIP
                     ITREEKPAVTAAPKK"
     exon            531..653
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
                     /inference="alignment:Splign:2.1.0"
     exon            654..995
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      972..977
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
     polyA_site      995
                     /gene="Cryab"
                     /gene_synonym="AACRYA"
ORIGIN      
        1 agaccctgtc ctggctccag agaacaagga tggggtgggt gggtgccact aggtgtggac
       61 agagagctag tgaaacaaga ccgtgacaag tcaccggcca gctcagccct gccccgtgtt
      121 tctcttttct tagctcagtg agtactgggt atgtgtcacc ctgccaaatc cctgatcaca
      181 agtccccatg aactgtcggg gagctgggat aataaaaccc ctgacatcac cgttccagaa
      241 gcttcacaag actgcatata taaggggcag gctgtagcag cggctgaagg agttgaccgg
      301 ctaaccgact ctacactcat ctagccatca tggacatagc catccaccac ccctggatcc
      361 ggcgtccctt ctttcctttc cactccccaa gccgcctctt tgaccagttc ttcggagagc
      421 acctgttgga gtctgacctc ttctctacag ccacttccct gagccccttc taccttcggc
      481 caccctcctt cctgcgggca cctagctgga ttgacactgg gctctcagag atgcgtatgg
      541 agaaggacag gttctctgtg aacctggacg tgaagcactt ctctccagag gaactcaaag
      601 tcaaggttct gggagacgtg attgaggtgc acggcaagca cgaagagcgc caggacgaac
      661 atggcttcat ctccagggag ttccacagga agtaccggat cccagccgac gtggatcctc
      721 tcaccattac ttcttccctg tcatcggatg gagtcctcac tgtgaatgga ccaaggaaac
      781 aggcctctgg ccctgagcgc accattccca tcacccgtga agagaagcct gctgtcactg
      841 cagcccctaa gaagtagatt ccctttcctc gttgcatttt ttaagacaag gaagtttccc
      901 atcagcgaat gaacatctgt gactagtgcc gaagcttact aatgctaagg gctggcccag
      961 attattaagc taataaaaaa tatcgttcag caacaaaaaa aaaaaaaaaa aaaaaaaaa
//
