LOCUS       NM_012534               1126 bp    mRNA    linear   ROD 04-APR-2024
DEFINITION  Rattus norvegicus crystallin, alpha A (Cryaa), transcript variant
            1, mRNA.
ACCESSION   NM_012534
VERSION     NM_012534.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Koteiche,H.A., Claxton,D.P., Mishra,S., Stein,R.A., McDonald,E.T.
            and Mchaourab,H.S.
  TITLE     Species-Specific Structural and Functional Divergence of
            alpha-Crystallins: Zebrafish alphaBa- and Rodent
            alphaA(ins)-Crystallin Encode Activated Chaperones
  JOURNAL   Biochemistry 54 (38), 5949-5958 (2015)
   PUBMED   26378715
  REMARK    GeneRIF: Rat crystallin alpha A a encode activated chaperones.
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Nahomi,R.B., Wang,B., Raghavan,C.T., Voss,O., Doseff,A.I.,
            Santhoshkumar,P. and Nagaraj,R.H.
  TITLE     Chaperone peptides of alpha-crystallin inhibit epithelial cell
            apoptosis, protein insolubilization, and opacification in
            experimental cataracts
  JOURNAL   J Biol Chem 288 (18), 13022-13035 (2013)
   PUBMED   23508955
  REMARK    GeneRIF: The alphaA- and alphaB-crystallin peptides inhibited
            stress-induced aggregation of four client proteins, and the
            alphaA-acetyl peptide was more effective than the native peptide
            against three of the client proteins.
REFERENCE   3  (bases 1 to 1126)
  AUTHORS   Wang,Y.H., Li,Y.C., Huo,S.J. and Yin,Z.Q.
  TITLE     Alpha-crystallin promotes rat olfactory ensheathing cells survival
            and proliferation through regulation of PI3K/Akt/mTOR signaling
            pathways
  JOURNAL   Neurosci Lett 531 (2), 170-175 (2012)
   PUBMED   23142719
  REMARK    GeneRIF: These findings indicated that alpha-crystallin positively
            regulated the activation of PI3K/Akt/mTOR signaling pathway and
            promote the proliferation and survival of cultured olfactory
            ensheathing cells
REFERENCE   4  (bases 1 to 1126)
  AUTHORS   Gangalum,R.K., Horwitz,J., Kohan,S.A. and Bhat,S.P.
  TITLE     alphaA-crystallin and alphaB-crystallin reside in separate
            subcellular compartments in the developing ocular lens
  JOURNAL   J Biol Chem 287 (50), 42407-42416 (2012)
   PUBMED   23071119
  REMARK    GeneRIF: alphaA-crystallin and alphaB-crystallin reside in separate
            subcellular compartments in the developing ocular lens
REFERENCE   5  (bases 1 to 1126)
  AUTHORS   Bhat,S.P., Nandy,P., Srinivasan,A., Cheng,D. and Sitay,A.
  TITLE     Expression of recombinant alpha Ains-crystallin and not alpha
            A-crystallin inhibits bacterial growth
  JOURNAL   Protein Eng 9 (8), 713-718 (1996)
   PUBMED   8875649
REFERENCE   6  (bases 1 to 1126)
  AUTHORS   Wang,K., Ma,W. and Spector,A.
  TITLE     Phosphorylation of alpha-crystallin in rat lenses is stimulated by
            H2O2 but phosphorylation has no effect on chaperone activity
  JOURNAL   Exp Eye Res 61 (1), 115-124 (1995)
   PUBMED   7556464
  REMARK    GeneRIF: Cryaa is a moonlighting protein that functions as a heat
            shock protein as well as a lens crystalline.
REFERENCE   7  (bases 1 to 1126)
  AUTHORS   Srinivasan,A.N., Nagineni,C.N. and Bhat,S.P.
  TITLE     alpha A-crystallin is expressed in non-ocular tissues
  JOURNAL   J Biol Chem 267 (32), 23337-23341 (1992)
   PUBMED   1429679
REFERENCE   8  (bases 1 to 1126)
  AUTHORS   Cenedella,R.J. and Fleschner,C.R.
  TITLE     Selective association of crystallins with lens 'native' membrane
            during dynamic cataractogenesis
  JOURNAL   Curr Eye Res 11 (8), 801-815 (1992)
   PUBMED   1424724
REFERENCE   9  (bases 1 to 1126)
  AUTHORS   Wen,Y., Shi,S.T., Unakar,N.J. and Bekhor,I.
  TITLE     Crystallin mRNA concentrations and distribution in lens of normal
            and galactosemic rats. Implications in development of sugar
            cataracts
  JOURNAL   Invest Ophthalmol Vis Sci 32 (5), 1638-1647 (1991)
   PUBMED   1707863
REFERENCE   10 (bases 1 to 1126)
  AUTHORS   Cohen,L.H., Westerhuis,L.W., de Jong,W.W. and Bloemendal,H.
  TITLE     Rat alpha-crystallin A chain with an insertion of 22 residues
  JOURNAL   Eur J Biochem 89 (1), 259-266 (1978)
   PUBMED   699911
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC126082.1.
            
            [WARNING] On Jun 12, 2024 this sequence was replaced by
            NM_012534.5.
            
            On Jan 28, 2014 this sequence version replaced NM_012534.3.
            
            Summary: This gene encodes subunit a, one of two subunits of
            alpha-crystallin, which is a high molecular weight, soluble
            aggregate and is a member of the small heat shock protein family.
            The encoded protein has been identified as a moonlighting protein
            based on its ability to perform mechanistically distinct functions.
            It acts as a molecular chaperone and is the major protein in the
            eye lens, maintaining the transparency and refractive index of the
            lens. Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jan 2014].
            
            Transcript Variant: This variant (1) encodes the shorter isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC126082.1, U47922.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN09345238, SAMN09345330
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s) :: PMID: 7556464
            RefSeq Select criteria          :: based on conservation,
                                               expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1126              BC126082.1         1-1126
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..1126
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya1"
                     /note="crystallin, alpha A"
                     /db_xref="GeneID:24273"
                     /db_xref="RGD:2413"
     exon            1..248
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya1"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..581
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     alpha-crystallin A chain; Crystallin, alpha polypeptide A"
                     /codon_start=1
                     /product="alpha-crystallin A chain isoform 1"
                     /protein_id="NP_036666.2"
                     /db_xref="GeneID:24273"
                     /db_xref="RGD:2413"
                     /translation="MDVTIQHPWFKRALGPFYPSRLFDQFFGEGLFEYDLLPFLSSTI
                     SPYYRQSLFRTVLDSGISEVRSDRDKFVIFLDVKHFSPEDLTVKVLEDFVEIHGKHNE
                     RQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIP
                     VSREEKPSSAPSS"
     exon            249..371
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..1104
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccagaggct cctgcctgac tcattgccag ccttcggtcc ctgcatcacc aaccagaaca
       61 tggacgtcac catccagcac ccttggttca agcgcgccct ggggcccttc taccccagcc
      121 gactgttcga ccagttcttc ggcgagggcc tttttgaata cgacctgctg cccttcctgt
      181 cttccaccat cagcccctac taccgccagt ctctcttccg cacagtgttg gactccggca
      241 tctctgaggt ccgatctgac cgggacaagt ttgtcatctt cttggatgtg aagcacttct
      301 ctcctgagga cctcaccgtg aaggtactgg aagatttcgt ggagatccat ggcaaacaca
      361 acgagaggca ggatgaccat ggctacattt cccgtgaatt tcaccgtcgc taccgtctgc
      421 cttccaatgt ggaccagtcc gccctctcct gctccttgtc tgcggatggc atgctgacct
      481 tctctggccc caaggtccag tctggcttgg atgctggcca cagcgagagg gccattcccg
      541 tgtcacggga ggagaagccc agctcggcac cctcgtcctg agcaggcctc gccttggttg
      601 tcccctgagg cccctgatcc atccagccca ggggccacag caaagagtct gccttcctga
      661 cttcttttct ttctctttgt ttcctttcca ctttctcaga gggctgagga tttgagagag
      721 tggcttaaag agcttggggg gtcttggcct gagatggctg cgggttcagg gtgacccagg
      781 ctcaacacca gccggtcaga gggaatgatg gcattgaact cttaagattt cctgtcctcc
      841 tggaaagtgg catcgagctc tgctccaaag gcagagtgaa tggtggctaa ccaaccccaa
      901 gagccctctg ccaagcccct ggatggcagc ctcccacccc ctttgcccac acttaccgca
      961 ggcgtatatg ctgggctcca acagtccgct tctctcatgc cctcttcctg tgactttctc
     1021 tactatgtag tatcgctcct ggggaccctg gtcacccatg agaatggagc ccctggcaga
     1081 caataaagag caggtgacaa gcaaaaaaaa aaaaaaaaaa aaaaaa
//
