LOCUS       NM_001047115            6034 bp    mRNA    linear   ROD 18-FEB-2024
DEFINITION  Rattus norvegicus CREB regulated transcription coactivator 1
            (Crtc1), mRNA.
ACCESSION   NM_001047115 XM_001070837
VERSION     NM_001047115.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Yan,P., Xue,Z., Li,D., Ni,S., Wang,C., Jin,X., Zhou,D., Li,X.,
            Zhao,X., Chen,X., Cui,W., Xu,D., Zhou,W. and Zhang,J.
  TITLE     Dysregulated CRTC1-BDNF signaling pathway in the hippocampus
            contributes to Abeta oligomer-induced long-term synaptic plasticity
            and memory impairment
  JOURNAL   Exp Neurol 345, 113812 (2021)
   PUBMED   34274327
  REMARK    GeneRIF: Dysregulated CRTC1-BDNF signaling pathway in the
            hippocampus contributes to Abeta oligomer-induced long-term
            synaptic plasticity and memory impairment.
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Mao,B., Li,L., Yan,M., Wong,C.K.C., Silvestrini,B., Li,C., Ge,R.,
            Lian,Q. and Cheng,C.Y.
  TITLE     F5-Peptide and mTORC1/rpS6 Effectively Enhance BTB Transport
            Function in the Testis-Lesson From the Adjudin Model
  JOURNAL   Endocrinology 160 (8), 1832-1853 (2019)
   PUBMED   31157869
  REMARK    GeneRIF: a specific deletion of mTOR in Sertoli cells was found to
            cause an upregulation of p-rpS6-S240/S244 (i.e., an activation of
            rpS6), but not total rpS6, and was associated with blood-testis
            barrier disruption, epithelial damage, loss of cell polarity, and
            infertility.
REFERENCE   3  (bases 1 to 6034)
  AUTHORS   Laguesse,S., Morisot,N., Phamluong,K. and Ron,D.
  TITLE     Region specific activation of the AKT and mTORC1 pathway in
            response to excessive alcohol intake in rodents
  JOURNAL   Addict Biol 22 (6), 1856-1869 (2017)
   PUBMED   27766766
  REMARK    GeneRIF: The PI3K/AKT pathway in the nucleus accumbens (NAc) of
            rodents plays a role in the molecular and behavioral adaptations
            that are induced by alcohol, and that in turn drives
            alcohol-drinking behaviors.
REFERENCE   4  (bases 1 to 6034)
  AUTHORS   Fukuchi,M., Kuwana,Y., Tabuchi,A. and Tsuda,M.
  TITLE     Balance between cAMP and Ca(2+) signals regulates expression levels
            of pituitary adenylate cyclase-activating polypeptide gene in
            neurons
  JOURNAL   Genes Cells 21 (8), 921-929 (2016)
   PUBMED   27383213
  REMARK    GeneRIF: depolarization (which produced a robust increase in Ca(2+)
            signals) together with cAMP signaling resulted in a synergistic
            induction of Pacap mRNA through calcineurin and its substrate,
            cAMP-response element-binding protein (CREB)-regulated
            transcription coactivator 1.
REFERENCE   5  (bases 1 to 6034)
  AUTHORS   Dubey,D. and Porter,B.E.
  TITLE     CRTC1 nuclear localization in the hippocampus of the
            pilocarpine-induced status epilepticus model of temporal lobe
            epilepsy
  JOURNAL   Neuroscience 320, 57-68 (2016)
   PUBMED   26844388
  REMARK    GeneRIF: Our results provide evidence that CREB cofactor CRTC1
            translocates into the nucleus of a distinct subset of hippocampal
            neurons during and following status epilepticus
REFERENCE   6  (bases 1 to 6034)
  AUTHORS   Li,S., Zhang,C., Takemori,H., Zhou,Y. and Xiong,Z.Q.
  TITLE     TORC1 regulates activity-dependent CREB-target gene transcription
            and dendritic growth of developing cortical neurons
  JOURNAL   J Neurosci 29 (8), 2334-2343 (2009)
   PUBMED   19244510
  REMARK    GeneRIF: TORC1 regulates activity-dependent CREB-target gene
            transcription and dendritic growth of developing cortical neurons.
REFERENCE   7  (bases 1 to 6034)
  AUTHORS   Kovacs,K.A., Steullet,P., Steinmann,M., Do,K.Q., Magistretti,P.J.,
            Halfon,O. and Cardinaux,J.R.
  TITLE     TORC1 is a calcium- and cAMP-sensitive coincidence detector
            involved in hippocampal long-term synaptic plasticity
  JOURNAL   Proc Natl Acad Sci U S A 104 (11), 4700-4705 (2007)
   PUBMED   17360587
  REMARK    GeneRIF: TORC1 is involved in L-LTP maintenance at the Schaffer
            collateral-CA1 synapses in the hippocampus
REFERENCE   8  (bases 1 to 6034)
  AUTHORS   Zhou,Y., Wu,H., Li,S., Chen,Q., Cheng,X.W., Zheng,J., Takemori,H.
            and Xiong,Z.Q.
  TITLE     Requirement of TORC1 for late-phase long-term potentiation in the
            hippocampus
  JOURNAL   PLoS One 1 (1), e16 (2006)
   PUBMED   17183642
  REMARK    GeneRIF: Overexpressing a dominant-negative form of TORC1 or
            down-regulating TORC1 expression prevented activity-dependent
            transcription of CREB target genes in cultured hippocampal neurons.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 6034)
  AUTHORS   Iourgenko,V., Zhang,W., Mickanin,C., Daly,I., Jiang,C.,
            Hexham,J.M., Orth,A.P., Miraglia,L., Meltzer,J., Garza,D.,
            Chirn,G.W., McWhinnie,E., Cohen,D., Skelton,J., Terry,R., Yu,Y.,
            Bodian,D., Buxton,F.P., Zhu,J., Song,C. and Labow,M.A.
  TITLE     Identification of a family of cAMP response element-binding protein
            coactivators by genome-scale functional analysis in mammalian cells
  JOURNAL   Proc Natl Acad Sci U S A 100 (21), 12147-12152 (2003)
   PUBMED   14506290
REFERENCE   10 (bases 1 to 6034)
  AUTHORS   Conkright,M.D., Canettieri,G., Screaton,R., Guzman,E., Miraglia,L.,
            Hogenesch,J.B. and Montminy,M.
  TITLE     TORCs: transducers of regulated CREB activity
  JOURNAL   Mol Cell 12 (2), 413-423 (2003)
   PUBMED   14536081
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000275.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001047115.3.
            
            On Sep 2, 2022 this sequence version replaced NM_001047115.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ538521.1, SRR8487230.77779.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132262, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-158               JACYVU010000275.1  2337572-2337729
            159-275             JACYVU010000275.1  2368035-2368151
            276-413             JACYVU010000275.1  2370738-2370875
            414-475             JACYVU010000275.1  2371645-2371706
            476-570             JACYVU010000275.1  2374459-2374553
            571-656             JACYVU010000275.1  2376139-2376224
            657-697             JACYVU010000275.1  2378414-2378454
            698-906             JACYVU010000275.1  2378752-2378960
            907-1040            JACYVU010000275.1  2384982-2385115
            1041-1343           JACYVU010000275.1  2385827-2386129
            1344-1445           JACYVU010000275.1  2386609-2386710
            1446-1532           JACYVU010000275.1  2389932-2390018
            1533-1713           JACYVU010000275.1  2390366-2390546
            1714-6034           JACYVU010000275.1  2391927-2396247
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p14"
     gene            1..6034
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /note="CREB regulated transcription coactivator 1"
                     /db_xref="GeneID:684527"
                     /db_xref="RGD:1589158"
     exon            1..158
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..1925
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /note="mucoepidermoid carcinoma translocated 1; transducer
                     of CREB protein 1; mucoepidermoid carcinoma translocated
                     protein 1 homolog; transducer of regulated cAMP response
                     element-binding protein 1"
                     /codon_start=1
                     /product="CREB-regulated transcription coactivator 1"
                     /protein_id="NP_001040580.1"
                     /db_xref="GeneID:684527"
                     /db_xref="RGD:1589158"
                     /translation="MATSNNPRKFSEKIALHNQKQAEETAAFEEVMKDLSLTRAARLQ
                     LQKSQYLQLGPSRGQYYGGSLPNVNQIGSSSMDLSFQTPFQSSGLDTSRTTRHHGLVD
                     RVYRERGRLGSPHRRPLSVDKHGRQADSCPYGTVYLSPPADTSWRRTNSDSALHQSTM
                     TPTQAESFTGGPQDAHQKRVLLLTVPGMEETGSETDKTLSKQSWDSKKAGSRPKSCEV
                     PGINIFPSADQENTAALIPATHNTGGSLPDLSTIHFPSPLPTPLDPEEPPFPALTSSG
                     STGSLAHLGVGGTGQGMNTPSSSPQRRPAVVSPLSLSTEARRQQAQQVPPTLSPLSPI
                     TQAVAMDALSLEQQLPYAFFTQAGSQQPPPQPQPPPPPPPVSQQQPPPPQVSVGLPQG
                     GPLLPSASLTRGPQLPPLAVTVPSTLPQSPTESPGQPPMGIDVTSAPALQYRTGAGSP
                     ATQSPTSPVSNQGFSPGSSPQHTSTLGSVFGDAYYEQQMTARQANALSRQLEQFNMME
                     NAISSSSLYNPGSTLNYSQAAMMGLSGSHGGLQDPQQLGYAGHGGIPNIILTVTGESP
                     PSLSKELSSTLAGVSDVSFDSDHQFPLDELKIDPLTLDGLHMLNDPDMVLADPATEDT
                     FRMDRL"
     exon            159..275
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            276..413
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            414..475
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            476..570
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            571..656
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            657..697
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            698..906
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1040
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1343
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1445
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1532
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1713
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..6034
                     /gene="Crtc1"
                     /gene_synonym="Mect1; TORC-1; TORC1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaggagtagg aggaggagga ggcggcgaga agatggcgac ttcgaacaat ccgcggaaat
       61 ttagcgagaa gatcgcactg cacaaccaga agcaggcgga ggagacggcg gccttcgagg
      121 aggtcatgaa ggacctgagc ctgacgcgag ccgcgcggct tcaactgcag aagtcgcagt
      181 atctccaact gggccccagc cgtggtcagt actatggcgg gtccctgccc aacgtgaacc
      241 agattggaag tagcagcatg gacctctcct tccagacccc ctttcagtcc tcaggcctgg
      301 acaccagtcg gaccacacga catcatgggc ttgtggacag agtatatcgt gagcgtggca
      361 gacttggctc cccacaccgc cgtcccctgt cagtagacaa gcatgggcga caggccgaca
      421 gctgccccta tggcaccgtg tacctctcgc ctcctgcgga caccagctgg aggaggacca
      481 actctgactc tgccctgcac cagagcacaa tgacacccac ccaggcagaa tccttcacag
      541 gcgggcccca ggatgcgcac cagaagagag tcttactgct cacagtccca ggaatggagg
      601 agaccggatc cgagacagac aagacccttt ctaagcagtc atgggactca aagaaggcgg
      661 gttccaggcc caagtcctgc gaagtccctg gaatcaacat ctttccatct gcagaccagg
      721 agaacacagc agccctgatc cctgctaccc acaacacagg aggctccctt cctgacctca
      781 gcaccatcca cttcccctcc ccactgccga ccccactgga ccccgaggag cctccattcc
      841 ctgctctcac cagctccggc agcaccggca gcctggctca tctaggcgtt ggcggcacag
      901 gccagggtat gaacaccccc agctcttctc cacagcgccg gccagcagtc gtcagccccc
      961 tgtccctgag cacagaggcc aggcggcagc aggcccagca ggtgccaccc accctgtctc
     1021 cactgtcacc catcactcag gccgtggcca tggatgccct gtcgttggag cagcagctgc
     1081 cctacgcctt cttcacccag gccggctcac agcagcctcc cccgcagccc cagccaccgc
     1141 ccccacctcc gcctgtatct caacagcagc caccaccccc acaagtgtct gtggggctcc
     1201 cccagggtgg cccactgctg cccagtgcca gcctgactcg ggggccccag ctgccaccac
     1261 tcgcagtcac tgtaccatcc actcttcccc agtcacctac agagagccca ggccagccac
     1321 caatggggat tgatgtcacc tcggcaccag ctctgcagta ccgcacgggt gcagggtcac
     1381 cagccaccca gtcccccacc tcaccggtct ccaaccaagg cttctccccc ggaagctccc
     1441 cacagcacac gtccacgctg ggcagcgtgt ttggggatgc gtactatgag cagcagatga
     1501 cagccaggca ggccaatgct ctgtcccgcc agctggagca gttcaacatg atggaaaatg
     1561 ccatcagctc cagcagcctg tacaacccag gttccacact caactactcc caggctgcca
     1621 tgatgggtct gagcgggagc cacggaggcc ttcaggaccc acagcaactc ggctacgcag
     1681 gccatggcgg aatccccaac atcatcctca ccgtgacagg ggagtcaccc ccgagcctct
     1741 ctaaggaact gagcagcaca ctggcagggg tcagtgatgt cagctttgat tcggaccacc
     1801 agtttccact ggacgagctg aaaattgatc ccctgaccct ggacggactt catatgttga
     1861 atgaccctga catggtcctc gctgacccag ctaccgagga caccttccga atggaccgcc
     1921 tgtgagtggc tttggtcagc ctgccacccg ctgctggtca gtctccaacg gcgctgcccc
     1981 aaactgggga tggcagtgcc gccccccttt gccaacggcc aagcttgtga ttctgagctt
     2041 gcaatgctgc ccggcgcccc ctgccagcca ccaccccggt tattcacctc ccatgatgcc
     2101 tggcgtgagg ccgttgtgta ctaggctggc tctctgtctg tccgtccatc cacctggggc
     2161 caggctgatg gccgaggctg tgagcacctg gccccatgga ctttccccat gctcactccc
     2221 tcgtccctta ctggggatgt gagagccctc cctcatcagt ggtccacttc caagataccc
     2281 aaagtgtcac tcccagatgt gctgtgcgaa gagggctagg gtgtgggcac agagcgcccc
     2341 agaggctgag gtgcgccatc cattcgactg ttctcagctg tcactgcctt ccttcccccc
     2401 accgccatcc ccagtcctgg tcaggtcatg catgcccact gtgcccaggg caaagacagc
     2461 agagtgaagg ggcagacctg gaccagaata aacactgttt ggatggctgc cttttatatt
     2521 cctgagcgct cttagagcgc aacaatcgac cggtggccaa atgcggcatg gacagagccc
     2581 actgaagcag ctggtgccct ccttggccac ccttgcccct gctgggcaac ggtcagcatg
     2641 gtggtgcaga gatagagggt cacaggccac ctgcgcattc tcctcgctct tccctgtccc
     2701 gtgaccagtc cccactgctc tttaagatct ggggctcggg aagcagcagc cctggtgggt
     2761 tctgcttttc cttcagtttc tgttggcgtt cggtagctga cccccccccc ccagacttgc
     2821 ctggcagcat gtgttacccc cagtctctcc ctgacctctt taaaactaac tggaacagca
     2881 gcctgaagca cttgcaagct gggggcacct gggctgctca ctcagacctg ggctgctcac
     2941 ccagacctgg gctgctcacc cagacctggg ctgctcaccc agacctgggc tgctcaccca
     3001 gacctgggct gctcactcag acctgggctg ctcacccaga cctgggctta ccggcactta
     3061 gtctcaactc caaagagccg ggtgggcaaa gactaggctg gatacaaggt cctggtggct
     3121 gggcgtgtct gtaagaacca caggacagat ttctaggaac ggccttggct ttcactgggg
     3181 cctccggaga accctctccc tctgagtttc cctaaggtgc cggccaggcc tcagcaggtg
     3241 gctggcgtcg gctggtgtct ctgcactaag attccctatg acaattgggg gctgtggatt
     3301 tggcttgtct cttctgggcc acttttggct tggaagctca gatccccacc accccaagtt
     3361 atagcttgac atgtcgtttg cctctgcccc acaggcccag gccttggctt gatcatttct
     3421 ctatggtgtt gcccactgcc catgctgggg gttggggagc acaagagctg tgtgtatctt
     3481 tctccaagca actaggtggc tgcctgcatg agaaagctgc tgccctttca tcaccgtccc
     3541 tttccccctg gacttgggga tacagcattg gtttgtccag ttcacctgtc caggtctcag
     3601 gcctttctat caccatggtg cgggcttgaa ggaaatccta gggttgttat tgttattatt
     3661 attatgatta ttattattat taaacatgca tggtggcaca cacctgttac cccagcactg
     3721 gagagctgga ggcaggagga ttaaaatatc aaggccagcc tgagctacat gagaccctgc
     3781 ttaaaaaagc gaaagagaaa gtacacatga atggggtctg attctctttg aattgtagaa
     3841 gtctgaacaa atggtcttaa caaggcagag aggctgatac agttcccagg caaggctctg
     3901 cttccaggtc actggccttc caagttctca gtgagccagg acaggacaga catggcccca
     3961 tggccctcct atgctggtgg cccggctttc aatgccctac agccgagagt gcatggagct
     4021 ttgttaatgt gacagttcat tgccaagttc tgggatgagg tgatatgccc ctgcctcagc
     4081 accccaggca gctgtgggca tctcacgacc cagccagggg ctagcactgg agtgcacgca
     4141 tgttccagag ggttcaccct gccctccccg gggcctggtt gccgtcgggg actcctggga
     4201 ggtgtctgtt ttctagacgt aggtcgcctg tccgataata cccctgtatt gccaagacat
     4261 cccaagatga cagctcaagg ccgtgggggc ctcagctggt gacacgcata tactgtgagc
     4321 cattgaactg ctgggagcag ccacccttca gcatgtgcat agacgctctg cccacatctg
     4381 tgctctcagg acagactgac acccgccctt gaggtcaggt gcatgagcca ggcctggcct
     4441 ttccccctca accttagcca tgtgtcacca gcttcagcca cgcctctcgg ccatgtgggt
     4501 gtccaaccca cttccgtgaa tcagaaatca gactggggca tcttgataac actatgccca
     4561 gctgcctcag tttaccttat ccttttaagg cctctggggc agtagttatc tacccaaagc
     4621 cccacaaaac agcaatgcaa ctggacccag cccctttctg tgtcctaaga aatggtttgt
     4681 cccctccacc caaggccctg tcacgtcccc tttctgcaac actatccaat gctgttctgt
     4741 gaagtccatt ggtacttagc ctagccttta gctctttgga agactcttat acaggggagc
     4801 cccctagcgg gacccatatg ctactgcaca gcacccaagg atggctgaca gctgtcacac
     4861 tgggcctggt gtcaagagtc aacagtcctg gcactccagt caggacaaga gcaggcaaaa
     4921 ggcgtgtgga ggaggcggca accccgcacc agtgtcggcc gctgttcttc tcacagtggg
     4981 cctagagttg gtccactgcc ttctgagttt cctgtttttg cagagaagat tcagcacagg
     5041 ctgagagggc tgctgggatc agggtcttga tggagctagc tgtgtgcgca ggcatggggt
     5101 tatatagttg tgccctgggc ctggctgtta tccctcaaga gctgtccagc tgaggacact
     5161 gtggtgcagt ctaccccacg tggtctgact ctagtgtggt ggtaacctgg tcctttttaa
     5221 acccctttca ccttggctcc agggggcggg gggaggggtc ctcttccttc agggatctgg
     5281 tgagtgattt ggagaccctg atcattcctg tcttaggcat ggccctcagt gtggcacagc
     5341 tgcctgccac ccattggtca tagccacatc tccttgtcag ggcagaagat ggagggactt
     5401 gtatcctctc gagcctgggt gctggggtac tcccaagacc atggggtgcc ctgaacctgt
     5461 tttcctcttt ctcaaccctg ctgggctcag gggcccaacc tcctgggcag ggtgggggca
     5521 gcagcagggc cccatgatgg taccagctac cctttctgca ctcagccttt ccttggggcc
     5581 agggatccat cacacggggc aaagtcttgc cccatctctt ggggctctag aatttcaggg
     5641 tgacccaggc ctagcctaat ccaacccagc agttctgatc acagagggac atgagaacag
     5701 ggctgtggct ttaaatccag aggccaggct ctgtttctca ggccccatct cagcccttag
     5761 ttggaaacag gtcgccatgg gctgggaagc ccctttgata tatgcaaatg gctggtccca
     5821 cattcccctc cccgtgccca ggctggctct gcccccagca tgcgcactcc gctgctgcgg
     5881 atgtctgtgg accgtgcgcg tgggcaccgc agttgggggt caggttgctc agggcacgca
     5941 cggccgcccg ccccaccttc tggcgcagac gcttctttgt atttttgcgc cctttgtaat
     6001 gaaatgtaat aaaaacccaa tgttttcgtc tggg
//
