LOCUS       NM_031987               2684 bp    mRNA    linear   ROD 13-OCT-2024
DEFINITION  Rattus norvegicus carnitine O-octanoyltransferase (Crot), mRNA.
ACCESSION   NM_031987
VERSION     NM_031987.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Le Borgne,F., Ben Mohamed,A., Logerot,M., Garnier,E. and
            Demarquoy,J.
  TITLE     Changes in carnitine octanoyltransferase activity induce alteration
            in fatty acid metabolism
  JOURNAL   Biochem Biophys Res Commun 409 (4), 699-704 (2011)
   PUBMED   21619872
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Ghosh,D. and Berg,J.M.
  TITLE     A proteome-wide perspective on peroxisome targeting signal
            1(PTS1)-Pex5p affinities
  JOURNAL   J Am Chem Soc 132 (11), 3973-3979 (2010)
   PUBMED   20178365
REFERENCE   3  (bases 1 to 2684)
  AUTHORS   Jogl,G., Hsiao,Y.S. and Tong,L.
  TITLE     Crystal structure of mouse carnitine octanoyltransferase and
            molecular determinants of substrate selectivity
  JOURNAL   J Biol Chem 280 (1), 738-744 (2005)
   PUBMED   15492013
REFERENCE   4  (bases 1 to 2684)
  AUTHORS   Cordente,A.G., Lopez-Vinas,E., Vazquez,M.I., Swiegers,J.H.,
            Pretorius,I.S., Gomez-Puertas,P., Hegardt,F.G., Asins,G. and
            Serra,D.
  TITLE     Redesign of carnitine acetyltransferase specificity by protein
            engineering
  JOURNAL   J Biol Chem 279 (32), 33899-33908 (2004)
   PUBMED   15155769
REFERENCE   5  (bases 1 to 2684)
  AUTHORS   Li,P., Li,J., Feng,X., Li,Z., Hou,R., Han,C. and Zhang,Y.
  TITLE     Gene expression profile of cardiomyocytes in hypertrophic heart
            induced by continuous norepinephrine infusion in the rats
  JOURNAL   Cell Mol Life Sci 60 (10), 2200-2209 (2003)
   PUBMED   14618266
REFERENCE   6  (bases 1 to 2684)
  AUTHORS   Ferdinandusse,S., Mulders,J., IJlst,L., Denis,S., Dacremont,G.,
            Waterham,H.R. and Wanders,R.J.
  TITLE     Molecular cloning and expression of human carnitine
            octanoyltransferase: evidence for its role in the peroxisomal
            beta-oxidation of branched-chain fatty acids
  JOURNAL   Biochem Biophys Res Commun 263 (1), 213-218 (1999)
   PUBMED   10486279
REFERENCE   7  (bases 1 to 2684)
  AUTHORS   Choi,S.J., Oh,D.H., Song,C.S., Roy,A.K. and Chatterjee,B.
  TITLE     Molecular cloning and sequence analysis of the rat liver carnitine
            octanoyltransferase cDNA, its natural gene and the gene promoter
  JOURNAL   Biochim Biophys Acta 1264 (2), 215-222 (1995)
   PUBMED   7495866
REFERENCE   8  (bases 1 to 2684)
  AUTHORS   Chatterjee,B., Song,C.S., Kim,J.M. and Roy,A.K.
  TITLE     Cloning, sequencing, and regulation of rat liver carnitine
            octanoyltransferase: transcriptional stimulation of the enzyme
            during peroxisome proliferation
  JOURNAL   Biochemistry 27 (25), 9000-9006 (1988)
   PUBMED   3233218
REFERENCE   9  (bases 1 to 2684)
  AUTHORS   Farrell,S.O., Fiol,C.J., Reddy,J.K. and Bieber,L.L.
  TITLE     Properties of purified carnitine acyltransferases of mouse liver
            peroxisomes
  JOURNAL   J Biol Chem 259 (21), 13089-13095 (1984)
   PUBMED   6436243
REFERENCE   10 (bases 1 to 2684)
  AUTHORS   Miyazawa,S., Ozasa,H., Osumi,T. and Hashimoto,T.
  TITLE     Purification and properties of carnitine octanoyltransferase and
            carnitine palmitoyltransferase from rat liver
  JOURNAL   J Biochem 94 (2), 529-542 (1983)
   PUBMED   6630173
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Nov 26, 2020 this sequence version replaced NM_031987.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U26033.1, BC074004.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                JAXUCZ010000004.1  26053124-26053153
            31-166              JAXUCZ010000004.1  26056465-26056600
            167-291             JAXUCZ010000004.1  26062870-26062994
            292-473             JAXUCZ010000004.1  26064453-26064634
            474-598             JAXUCZ010000004.1  26064824-26064948
            599-707             JAXUCZ010000004.1  26068626-26068734
            708-801             JAXUCZ010000004.1  26074416-26074509
            802-927             JAXUCZ010000004.1  26075222-26075347
            928-1029            JAXUCZ010000004.1  26076767-26076868
            1030-1113           JAXUCZ010000004.1  26078891-26078974
            1114-1221           JAXUCZ010000004.1  26079048-26079155
            1222-1352           JAXUCZ010000004.1  26079389-26079519
            1353-1476           JAXUCZ010000004.1  26082421-26082544
            1477-1555           JAXUCZ010000004.1  26083335-26083413
            1556-1649           JAXUCZ010000004.1  26083499-26083592
            1650-1769           JAXUCZ010000004.1  26083689-26083808
            1770-2684           JAXUCZ010000004.1  26087143-26088057
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q12"
     gene            1..2684
                     /gene="Crot"
                     /note="carnitine O-octanoyltransferase"
                     /db_xref="GeneID:83842"
                     /db_xref="RGD:70908"
     exon            1..30
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            31..166
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..1890
                     /gene="Crot"
                     /EC_number="2.3.1.137"
                     /note="COT"
                     /codon_start=1
                     /product="peroxisomal carnitine O-octanoyltransferase"
                     /protein_id="NP_114193.2"
                     /db_xref="GeneID:83842"
                     /db_xref="RGD:70908"
                     /translation="MENQLAKSIEERTFQYQDSLPPLPVPSLEESLKKYLESVKPFAN
                     EDEYKKTEEIVQKFQDGVGKTLHQKLLERAKGKRNWLEEWWLNVAYLDVRIPSQLNVN
                     FVGPSPHFEHYWPAREGTQLERGSILLWHNLNYWQLLRREKLPVHKSGNTPLDMNQFR
                     MLFSTCKVPGITRDSIMNYFKTESEGHCPTHIAVLCRGRAFVFDVLHDGCLITPPELL
                     RQLTYIYQKCWNEPVGPSIAALTSEERTRWAKAREYLIGLDPENLTLLEKIQSSLFVY
                     SIEDTSPHATPENFSQVFEMLLGGDPAVRWGDKSYNLISFANGIFGCSCDHAPYDAMV
                     MVNIAHYVDEKLLETEGRWKGSEKVRDIPLPEELAFTVDEKILNDVYQAKAQHLKAAS
                     DLQIAASTFTSFGKKLTKKEALHPDTFIQLALQLAYYRLHGRPGCCYETAMTRYFYHG
                     RTETVRSCTVEAVRWCQSMQDPSASLLERQQKMLDAFAKHNKMMRDCSHGKGFDRHLL
                     GLLLIAKEEGLPVPELFEDPLFSRSGGGGNFVLSTSLVGYLRIQGVVVPMVHNGYGFF
                     YHIRDDRFVVTCSSWRSCLETDAEKLVEMIFHAFHDMIHLMNTAHL"
     exon            167..291
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            292..473
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            474..598
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            599..707
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            708..801
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            802..927
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1029
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1113
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1221
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1352
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1476
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1555
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1649
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1769
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..2684
                     /gene="Crot"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagtgcagag agccaagccg ggtgcaggag ttttcttact gtgactatac catggaaaat
       61 caattggcta agtcaattga agaacgaaca ttccagtacc aggactctct tccgcccttg
      121 cccgttcctt cgcttgaaga atcactgaag aagtaccttg agtcagtgaa gccatttgca
      181 aatgaagacg aatacaagaa aactgaagaa atagttcaaa agtttcaaga tggagttggc
      241 aagacattgc atcagaagtt acttgaaagg gctaaaggaa aaagaaactg gctggaagag
      301 tggtggctca atgtcgccta cttggatgtg cgtattccat cacaactgaa cgtgaacttt
      361 gtgggtccgt ctccccactt tgaacactac tggcctgcaa gggaaggcac tcagttggaa
      421 agaggaagca tactactgtg gcacaacttg aactactggc agctgctaag aagagaaaaa
      481 ttgcctgtac ataaatctgg aaatactcct ctagacatga accaattccg gatgctgttt
      541 tctacctgca aggttccggg aatcactaga gattcgatta tgaattattt taagactgag
      601 agcgaggggc attgtccgac ccacattgcc gtgctgtgtc gaggcagagc gtttgtcttc
      661 gatgtcctcc atgacggttg tttgatcacc ccaccagaac ttctcagaca actgacatac
      721 atctaccaga aatgctggaa tgaacctgtt gggcccagta tagcggcatt aaccagtgag
      781 gagcgaactc ggtgggcgaa ggcaagagaa tatctgattg gtcttgatcc agagaacttg
      841 actttattag aaaaaattca atccagttta tttgtgtatt ccatagaaga caccagtcca
      901 catgcaaccc cagaaaattt ttctcaggtc tttgaaatgc ttcttggtgg agatccagca
      961 gtgcgctggg gtgacaagtc ctataatctg atttcctttg ctaacggaat atttggctgt
     1021 agctgtgatc atgctcctta tgatgcaatg gttatggtga acattgctca ctatgttgat
     1081 gagaagctcc tagagacgga agggagatgg aagggttcag aaaaagtccg ggatataccg
     1141 ttgccagagg agctggcttt cactgtggat gagaagatac tgaatgacgt ctaccaagcc
     1201 aaagcccaac acctcaaagc agcatctgat ttacagatag cagcatctac cttcacatct
     1261 tttggcaaaa agctcactaa gaaggaggcc cttcaccctg acacctttat tcagctcgct
     1321 cttcagctcg cctactacag acttcatgga cgccccggtt gctgctatga aacagctatg
     1381 acaagatact tttaccatgg ccgaacagag actgtgcgat cttgtacagt ggaggccgtc
     1441 aggtggtgcc agtccatgca ggatccttct gccagtctcc ttgaacgtca gcaaaagatg
     1501 ttagacgctt ttgcaaagca taacaagatg atgagagatt gttcccatgg aaaaggattt
     1561 gaccgtcacc ttttaggcct tttgctcata gcaaaagagg aaggcctccc tgttccagaa
     1621 ctgtttgagg atccactttt ctccagaagt ggaggaggtg ggaattttgt gctgtcaaca
     1681 agtctggttg gttacttacg aattcaggga gtcgtggttc ccatggtaca taatggatac
     1741 ggctttttct accacatcag agatgacagg tttgtggtga catgttcatc ctggaggtca
     1801 tgtcttgaga ctgatgcaga aaagttagtg gagatgattt ttcatgcttt ccacgatatg
     1861 atacatctga tgaacacggc tcatctttag agactcagag acatacaggt cacagaaact
     1921 gggtacggag aatgggatgg tgatacgaca tggaaggaat gttgacttaa aggaaacctg
     1981 ttaatgcagg gattagagag ggatgcactc tagatttatt ctaccttaaa gccttctgtg
     2041 ttgcaacagc aatgcaaact cagacatagt gaatagaact atgcaatgtt ttaagcctca
     2101 acaatgcaca tctgtatatt ttaacaatac aaatcctact ctaatgttaa aatatttttg
     2161 ttggcacatg tgtaggttgc aagtcctctg tgaacataat tatagagtat ttctcaagca
     2221 ctttaatact ttctaatggc cagagggtat aaaacccatg gttagatgct aatttccctg
     2281 acatcagtgc cttctacatc cagcacagga gtacaagcct atgagatttc atgggaaaac
     2341 cactattgtt caatattgat ctaaaatagc tcctttgaac agacaaaagt atcaagttgt
     2401 attagaaaag aatattagca aaactcatta tgatatgttg taattaattt tgtgaatata
     2461 aaatcaaaac acttccattt aaatctactt ggtagagtta gtggctttta aagggttaaa
     2521 tgtcgagtat gattctcaga actttataat tatttcccac tgttattcaa aatgttagca
     2581 tatagacatt ctcccattgt aattcagtgt ttatattctc aaagaataaa gcatccagaa
     2641 tccttgtaat ttctcattta ttttcaataa aaatgattcc tgat
//
