LOCUS       NM_017072               4838 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus carbamoyl-phosphate synthase 1 (Cps1), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_017072 XM_346852
VERSION     NM_017072.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Cao,Y., Ding,W., Zhang,J., Gao,Q., Yang,H., Cao,W., Wang,Z.,
            Fang,L. and Du,R.
  TITLE     Significant Down-Regulation of Urea Cycle Generates Clinically
            Relevant Proteomic Signature in Hepatocellular Carcinoma Patients
            with Macrovascular Invasion
  JOURNAL   J Proteome Res 18 (5), 2032-2044 (2019)
   PUBMED   30901224
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Khoja,S., Nitzahn,M., Hermann,K., Truong,B., Borzone,R., Willis,B.,
            Rudd,M., Palmer,D.J., Ng,P., Brunetti-Pierri,N. and Lipshutz,G.S.
  TITLE     Conditional disruption of hepatic carbamoyl phosphate synthetase 1
            in mice results in hyperammonemia without orotic aciduria and can
            be corrected by liver-directed gene therapy
  JOURNAL   Mol Genet Metab 124 (4), 243-253 (2018)
   PUBMED   29801986
REFERENCE   3  (bases 1 to 4838)
  AUTHORS   Martin,J., Balmer,M.L., Rajendran,S., Maurhofer,O., Dufour,J.F. and
            St-Pierre,M.V.
  TITLE     Nutritional stress exacerbates hepatic steatosis induced by
            deletion of the histidine nucleotide-binding (Hint2) mitochondrial
            protein
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 310 (7), G497-G509 (2016)
   PUBMED   26767982
REFERENCE   4  (bases 1 to 4838)
  AUTHORS   Carter,W.G., Vigneswara,V., Newlaczyl,A., Wayne,D., Ahmed,B.,
            Saddington,S., Brewer,C., Raut,N., Gerdes,H.K., Erdozain,A.M.,
            Tooth,D., Bolt,E.L., Osna,N.A., Tuma,D.J. and Kharbanda,K.K.
  TITLE     Isoaspartate, carbamoyl phosphate synthase-1, and carbonic
            anhydrase-III as biomarkers of liver injury
  JOURNAL   Biochem Biophys Res Commun 458 (3), 626-631 (2015)
   PUBMED   25684186
  REMARK    GeneRIF: The results suggest that elevated isoaspartate and CPS-1,
            and reduced CA-III levels could serve as biomarkers of
            hepatocellular injury.
REFERENCE   5  (bases 1 to 4838)
  AUTHORS   Takakusa,H., Mohar,I., Kavanagh,T.J., Kelly,E.J., Kaspera,R. and
            Nelson,S.D.
  TITLE     Protein tyrosine nitration of mitochondrial carbamoyl phosphate
            synthetase 1 and its functional consequences
  JOURNAL   Biochem Biophys Res Commun 420 (1), 54-60 (2012)
   PUBMED   22402285
REFERENCE   6  (bases 1 to 4838)
  AUTHORS   Brandt,M.A. and Powers-Lee,S.G.
  TITLE     The interaction of cardiolipin with rat liver carbamoyl phosphate
            synthetase I
  JOURNAL   Arch Biochem Biophys 290 (1), 14-20 (1991)
   PUBMED   1898084
REFERENCE   7  (bases 1 to 4838)
  AUTHORS   Moorman,A.F., de Boer,P.A., Charles,R. and Lamers,W.H.
  TITLE     Diet- and hormone-induced reversal of the carbamoylphosphate
            synthetase mRNA gradient in the rat liver lobulus
  JOURNAL   FEBS Lett 276 (1-2), 9-13 (1990)
   PUBMED   1979948
REFERENCE   8  (bases 1 to 4838)
  AUTHORS   de Groot,C.J., ten Voorde,G.H., van Andel,R.E., te Kortschot,A.,
            Gaasbeek Janzen,J.W., Wilson,R.H., Moorman,A.F., Charles,R. and
            Lamers,W.H.
  TITLE     Reciprocal regulation of glutamine synthetase and
            carbamoylphosphate synthetase levels in rat liver
  JOURNAL   Biochim Biophys Acta 908 (3), 231-240 (1987)
   PUBMED   2882780
REFERENCE   9  (bases 1 to 4838)
  AUTHORS   Nyunoya,H., Broglie,K.E., Widgren,E.E. and Lusty,C.J.
  TITLE     Characterization and derivation of the gene coding for
            mitochondrial carbamyl phosphate synthetase I of rat
  JOURNAL   J Biol Chem 260 (16), 9346-9356 (1985)
   PUBMED   2991241
REFERENCE   10 (bases 1 to 4838)
  AUTHORS   Karsai,T., Rady,P., Nagy,Z. and Szondy,Z.
  TITLE     Effect of dimethylnitrosamine upon carbamoyl phosphate and
            ornithine utilization in rat liver
  JOURNAL   Anticancer Res 5 (4), 441-444 (1985)
   PUBMED   2864015
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000009.1.
            
            On Apr 15, 2016 this sequence version replaced NM_017072.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643288.37897.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-317               JAXUCZ010000009.1  76063863-76064179
            318-461             JAXUCZ010000009.1  76074561-76074704
            462-571             JAXUCZ010000009.1  76093064-76093173
            572-716             JAXUCZ010000009.1  76096141-76096285
            717-806             JAXUCZ010000009.1  76097184-76097273
            807-863             JAXUCZ010000009.1  76099165-76099221
            864-956             JAXUCZ010000009.1  76102210-76102302
            957-1046            JAXUCZ010000009.1  76105416-76105505
            1047-1175           JAXUCZ010000009.1  76108924-76109052
            1176-1282           JAXUCZ010000009.1  76109567-76109673
            1283-1421           JAXUCZ010000009.1  76110547-76110685
            1422-1499           JAXUCZ010000009.1  76112062-76112139
            1500-1598           JAXUCZ010000009.1  76114800-76114898
            1599-1694           JAXUCZ010000009.1  76116597-76116692
            1695-1884           JAXUCZ010000009.1  76117995-76118184
            1885-2042           JAXUCZ010000009.1  76119437-76119594
            2043-2171           JAXUCZ010000009.1  76121333-76121461
            2172-2316           JAXUCZ010000009.1  76123946-76124090
            2317-2527           JAXUCZ010000009.1  76125548-76125758
            2528-2726           JAXUCZ010000009.1  76127171-76127369
            2727-2903           JAXUCZ010000009.1  76129256-76129432
            2904-3022           JAXUCZ010000009.1  76132400-76132518
            3023-3164           JAXUCZ010000009.1  76148887-76149028
            3165-3230           JAXUCZ010000009.1  76150257-76150322
            3231-3294           JAXUCZ010000009.1  76150747-76150810
            3295-3476           JAXUCZ010000009.1  76152025-76152206
            3477-3671           JAXUCZ010000009.1  76159182-76159376
            3672-3739           JAXUCZ010000009.1  76159807-76159874
            3740-3815           JAXUCZ010000009.1  76161431-76161506
            3816-3893           JAXUCZ010000009.1  76165061-76165138
            3894-4001           JAXUCZ010000009.1  76167643-76167750
            4002-4091           JAXUCZ010000009.1  76169297-76169386
            4092-4262           JAXUCZ010000009.1  76170656-76170826
            4263-4337           JAXUCZ010000009.1  76172917-76172991
            4338-4436           JAXUCZ010000009.1  76175570-76175668
            4437-4496           JAXUCZ010000009.1  76182131-76182190
            4497-4609           JAXUCZ010000009.1  76183238-76183350
            4610-4739           JAXUCZ010000009.1  76184723-76184852
            4740-4838           JAXUCZ010000009.1  76185735-76185833
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q32"
     gene            1..4838
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /note="carbamoyl-phosphate synthase 1"
                     /db_xref="GeneID:497840"
                     /db_xref="RGD:2395"
     exon            1..317
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            318..461
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             336..4838
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /EC_number="6.3.4.16"
                     /note="carbamoyl-phosphate synthase [ammonia],
                     mitochondrial; carboamyl-phosphate synthetase 1;
                     carbamoyl-phosphate synthetase 1, mitochondrial; CPSase I;
                     carbamoyl-phosphate synthetase I; carbamyl phosphate
                     synthetase I; carbamyl-phosphate synthetase"
                     /codon_start=1
                     /product="carbamoyl-phosphate synthase [ammonia],
                     mitochondrial precursor"
                     /protein_id="NP_058768.1"
                     /db_xref="GeneID:497840"
                     /db_xref="RGD:2395"
                     /translation="MTRILTACKVVKTLKSGFGLANVTSKRQWDFSRPGIRLLSVKAQ
                     TAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYSEALTDPAYKGQILTMANPIIG
                     NGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSHDYNHWLATKSLGQWLQEEKVPA
                     IYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVKVFGKGNPT
                     KVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMDYDGLLIAGGPGNPALAQPLIQ
                     NVKKILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITA
                     QNHGYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFD
                     SFFSLIKKGKGTTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVK
                     AMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQFVTEVIKAERPDGLILGM
                     GGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFA
                     VESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLMDLGTKAFAMTNQILVER
                     SVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRR
                     TSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAK
                     IALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEV
                     MAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLRKELSEPSSTRIYAIAK
                     ALENNMSLDEIVKLTSIDKWFLYKMRDILNMDKTLKGLNSESVTEETLRQAKEIGFSD
                     KQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFD
                     EHGIMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKL
                     YFEELSLERILDIYHQEACNGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAED
                     RSIFSAVLDELKVAQAPWKAVNTLNEALEFANSVGYPCLLRPSYVLSGSAMNVVFSED
                     EMKRFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKEGRVISHAISEHVEDAGVHSG
                     DATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASR
                     SFPFVSKTLGVDFIDVATKVMIGESVDEKHLPTLEQPIIPSDYVAIKAPMFSWPRLRD
                     ADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVA
                     EQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVI
                     NLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVQKARTVDSKSLFHYRQY
                     SAGKAA"
     exon            462..571
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            572..716
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            717..806
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            807..863
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            864..956
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1046
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1175
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1282
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1421
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1499
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1598
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1694
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1884
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2042
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2171
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2172..2316
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2527
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2726
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2727..2903
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..3022
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3164
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3230
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3231..3294
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3295..3476
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3477..3671
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..3739
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3740..3815
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3816..3893
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3894..4001
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4002..4091
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4092..4262
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4263..4337
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4338..4436
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4437..4496
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4497..4609
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4610..4739
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4740..4838
                     /gene="Cps1"
                     /gene_synonym="cps; cps-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagtccagt atcaaggtag cggagtagtt attttcctag acacagcaca gagtttgcaa
       61 gtctgaagaa aagattaaaa aagggctgtc ctgacgtggg cctggacatg gaagtcttgt
      121 ttccagccgt cctgagtcta agcttggacg gtcagaaatg tgcttgcttt gattctgatg
      181 aggcccacct ttccttagct gctcacggtc aagatttgga gaagaagcaa cagtggtgag
      241 ctgagtaatt cattggattt cgatgaggag ttcttgagaa aactgattac agatccttat
      301 aactttaata taatttgtca caaaacatct tcaaaatgac gaggattttg acagcatgca
      361 aagtggtgaa gacactgaag agtggctttg gtttggccaa tgtgacttcg aagcgacagt
      421 gggacttctc tagacctggc atcaggctcc tttctgtgaa ggcacagaca gcacacattg
      481 ttctggaaga tggaactaag atgaagggct actcctttgg ccatccctcc tcggttgctg
      541 gcgaagtggt ttttaatact ggcttaggag ggtactcgga agcacttact gatcctgcct
      601 acaaggggca gatcctcacc atggccaacc ctatcattgg gaatggtggg gcccctgata
      661 caacggcacg tgatgaactg ggactgaata agtacatgga gtctgatgga atcaaggtgg
      721 cgggtctgct ggtgctgaat tacagtcatg actacaacca ctggctggcc accaagagtc
      781 tgggtcagtg gctgcaggag gagaaggtcc ctgcaattta tggagtggat acaagaatgc
      841 tgactaaaat aattcgggat aagggtacca tgcttgggaa gattgagttt gagggccagt
      901 ctgtggactt tgtggatcct aataagcaga atttgattgc cgaggtttca accaaggatg
      961 tcaaggtgtt tggcaaagga aaccccacga aagtggtagc cgtggactgt gggataaaaa
     1021 acaatgtcat ccgcctgcta gttaagcgag gagcggaagt gcatttggtc ccctggaatc
     1081 atgacttcac ccagatggac tatgacggac ttctgatcgc tggaggacct gggaacccag
     1141 ctctggcaca gccactaatt cagaacgtga agaagatttt ggagagtgac cgcaaagagc
     1201 cgttgtttgg aatcagtaca ggaaacataa taacaggatt ggctgctggc gccaaatcct
     1261 acaagatgtc catggccaac agaggacaga accaacctgt tttgaatatc acaaacagac
     1321 aggctttcat aactgctcag aatcatggct atgctctgga caacaccctc cctgctggct
     1381 ggaaaccact gtttgtcaat gtcaatgacc aaacaaatga ggggattatg catgagagca
     1441 aacccttctt cgctgtgcag ttccacccag aggtcagccc ggggccaaca gacactgagt
     1501 acctatttga ttccttcttc tcgctgataa agaagggcaa aggcaccacc attacctcag
     1561 ttctgcccaa gccagcattg gtggcatctc gagtcgaggt ttccaaggtc cttatcctag
     1621 gatcaggagg cctgtccatt ggtcaggcag gtgaatttga ttactccgga tctcaggctg
     1681 taaaagccat gaaggaagaa aacgtcaaaa cagtcctgat gaacccgaac attgcatccg
     1741 tgcagaccaa cgaggtggga ttgaagcagg cagatgcagt ctactttctc cctatcaccc
     1801 cccagtttgt cacagaagtt atcaaggctg aacggcccga tgggttaatt ctgggcatgg
     1861 gtggccagac agctctgaac tgtggagtgg aactattcaa gaggggtgtg ctcaaggaat
     1921 atggtgtgaa ggtcttggga acatcggtcg agtccattat ggccacagaa gacaggcagc
     1981 ttttctcaga caagctgaat gagatcaacg agaagattgc tcctagcttt gcagtggaat
     2041 caatggagga tgccttgaag gcagcagaca ccattggcta ccctgtgatg attcggtctg
     2101 cgtatgctct gggtgggtta ggctccggca tctgtcccaa caaggagacc ttaatggatc
     2161 ttggcacaaa ggcatttgct atgaccaacc agatcctggt ggagcggtca gtgacaggtt
     2221 ggaaagaaat agaatatgaa gttgtccgag atgcggatga taactgtgtc acagtctgta
     2281 acatggagaa tgttgacgcc atgggtgttc acacaggtga ttcagttgtt gtggccccgg
     2341 cccagaccct ctccaatgca gagttccaga tgttgagacg cacttcaatc aatgttgttc
     2401 gccacttggg cattgtgggt gaatgcaaca ttcagtttgc tcttcacccc acttccatgg
     2461 aatactgtat cattgaagtg aatgccaggc tctccaggag ttctgccctg gcctccaagg
     2521 ccactggcta cccactggcg ttcatcgcag caaagatcgc tctaggaatc ccacttccag
     2581 aaatcaagaa tgttgtgtct gggaagacct cagcctgctt cgaacctagc ctggattaca
     2641 tggtgaccaa gattcctcgc tgggaccttg accgttttca tggaacatcc agccgaattg
     2701 gtagctcaat gaaaagtgta ggcgaggtca tggccattgg tcgcaccttt gaggaaagtt
     2761 tccagaaggc tctgaggatg tgccatccat ctgtggatgg gttcactccc cgtctcccaa
     2821 tgaataagga atggccagca aacctggatc tgaggaagga gctgtctgaa ccctccagca
     2881 cccgcatcta tgccattgct aaggccttgg aaaacaacat gtcccttgac gaaatcgtga
     2941 agctcacatc cattgacaag tggtttttgt ataagatgcg tgacatttta aacatggata
     3001 agacactgaa agggcttaac agtgagtctg ttacagaaga aactctgaga caggccaaag
     3061 agattgggtt ctcagacaag cagatttcaa aatgtttagg actgaccgag gctcagacaa
     3121 gagagctgag attgaagaaa aacatccacc cttgggttaa acagattgat acattggctg
     3181 cagaataccc atcagtgaca aattacctgt atgttaccta caatggccag gagcacgaca
     3241 tcaaatttga tgaacatgga ataatggtgc tgggctgtgg cccataccac attggcagca
     3301 gtgtggaatt tgattggtgt gctgtctcca gtatccgcac actgcgccaa cttggcaaga
     3361 agacggtggt ggtgaattgt aacccggaga ctgtgagcac tgactttgat gagtgtgaca
     3421 aactctactt tgaagagctg tctttggaga gaatcctaga tatctaccac caggaggcat
     3481 gtaatggctg catcatatca gtcgggggcc agattccaaa caacttggcg gttccgctat
     3541 acaagaacgg tgtcaagatc atgggtacca gtcctctgca gatcgatagg gctgaggatc
     3601 gctccatctt ctcggctgtc ttagatgagc tgaaggtggc ccaggctcct tggaaagctg
     3661 ttaacacttt gaacgaggcg ctggaatttg caaactctgt gggctatccc tgcttactga
     3721 gaccttccta tgttttgagt gggtctgcca tgaacgtggt attctctgag gatgagatga
     3781 aaaggttcct tgaggaggcc actcgagtct ctcaggaaca cccagtggtg ctgaccaagt
     3841 ttattgaggg ggctcgggaa gtggagatgg acgctgttgg caaagaagga cgggtcatct
     3901 cccatgccat ctctgaacat gttgaagatg caggtgtcca ctcaggggat gccacactga
     3961 tgctacctac gcagaccatc agccaaggag ccattgaaaa ggtgaaggat gccacacgaa
     4021 agattgcaaa ggcttttgcc atctctgggc cattcaatgt tcagtttctt gtcaaaggaa
     4081 atgatgtctt ggtgattgag tgcaatctga gagcctctcg atccttcccc tttgtctcca
     4141 agactcttgg ggtggacttc attgatgtgg ccaccaaggt gatgatcgga gagagtgttg
     4201 atgagaagca tctacccaca ctggaacaac ccatcatccc ctctgactat gttgccatta
     4261 aggctcccat gttttcctgg ccccgactga gggatgctga tcctattctg agatgtgaga
     4321 tggcttctac tggagaggtg gcgtgttttg gtgagggcat tcatacagcc ttcctaaagg
     4381 caatgctgtc cacagggttt aagatacctc agaagggcat tctgattggc atccagcaat
     4441 cattccgtcc aagattcctt ggtgttgctg agcagttaca caatgaaggt ttcaagcttt
     4501 ttgccacaga agccacatca gactggctca acgccaacaa tgttcctgcc accccagtgg
     4561 cttggccatc tcaggaagga cagaatccca gcctctcttc catcagaaag ttgataagag
     4621 acggaagcat tgacctagtg attaacctcc ccaataacaa caccaaattt gtccatgata
     4681 attatgtgat tcggaggaca gctgtggaca gtggaattgc tctgctcacc aatttccagg
     4741 tgaccaaact ttttgctgag gctgtgcaga aagctcgtac tgtggactcc aagagtctgt
     4801 tccactacag gcagtacagc gctggaaagg cagcatag
//
