LOCUS       NM_012533               1404 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus carboxypeptidase B1 (Cpb1), mRNA.
ACCESSION   NM_012533
VERSION     NM_012533.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Yu,S.L., Han,S., Kim,H.R., Park,J.W., Jin,D.I. and Kang,J.
  TITLE     Phosphorylation of carboxypeptidase B1 protein regulates beta-cell
            proliferation
  JOURNAL   Int J Mol Med 40 (5), 1397-1404 (2017)
   PUBMED   28949379
  REMARK    GeneRIF: The dephosphorylation of CPB1 induced beta-cell
            proliferation. We thus surmise that the altered PTM of CPB1 is
            associated with pancreatic regeneration.
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Li,S., Zhang,L., Wu,Q., Xin,A., Zhao,J. and Fan,L.
  TITLE     Increasing the refolding efficiency in vitro by site-directed
            mutagenesis of Cys383 in rat procarboxypeptidase B
  JOURNAL   Enzyme Microb Technol 49 (2), 139-145 (2011)
   PUBMED   22112400
  REMARK    GeneRIF: Mutants C383G, C383A had higher k(cat)/K(m) values which
            indicated increased catalytic abilities. And both had higher
            thermal stability.
REFERENCE   3  (bases 1 to 1404)
  AUTHORS   Oliveira,E.B., Souza,L.L., Sivieri,D.O. Jr., Bispo-da-Silva,L.B.,
            Pereira,H.J., Costa-Neto,C.M., Sousa,M.V. and Salgado,M.C.
  TITLE     Carboxypeptidase B and other kininases of the rat coronary and
            mesenteric arterial bed perfusates
  JOURNAL   Am J Physiol Heart Circ Physiol 293 (6), H3550-H3557 (2007)
   PUBMED   17906107
  REMARK    GeneRIF: Distinct bradykinin-processing pathways operate in the
            perfusates of rat mesenteric/coronary beds, with a substantial
            participation of a des-Arg(9)-BK-forming enzyme identical to
            pancreatic CPB.
REFERENCE   4  (bases 1 to 1404)
  AUTHORS   Wang,D.J., Miao,L., Chen,H., Li,Y.Y., Chen,H.P. and Fang,H.Q.
  TITLE     [Expression, purification and characterization of rat
            procarboxypeptidase B in Pichia pastoris]
  JOURNAL   Sheng Wu Gong Cheng Xue Bao 23 (1), 61-66 (2007)
   PUBMED   17366889
  REMARK    GeneRIF: Data show that specific activity of recombinant CPB is
            110u/mg.
REFERENCE   5  (bases 1 to 1404)
  AUTHORS   Xiao-Yan,Z., Su-Xia,L. and Qin-Sheng,Y.
  TITLE     The renaturation of procarboxypeptidase B by urea gradient gel
            filtration and some properties of recombinant carboxypeptidase B
  JOURNAL   Protein Pept Lett 12 (7), 671-676 (2005)
   PUBMED   16522183
  REMARK    GeneRIF: optimum of procarboxypeptidase B activity was achieved at
            pH 7-9
REFERENCE   6  (bases 1 to 1404)
  AUTHORS   Clark,J.S., Jeffs,B., Davidson,A.O., Lee,W.K., Anderson,N.H.,
            Bihoreau,M.T., Brosnan,M.J., Devlin,A.M., Kelman,A.W.,
            Lindpaintner,K. and Dominiczak,A.F.
  TITLE     Quantitative trait loci in genetically hypertensive rats. Possible
            sex specificity
  JOURNAL   Hypertension 28 (5), 898-906 (1996)
   PUBMED   8901842
REFERENCE   7  (bases 1 to 1404)
  AUTHORS   Dubay,C., Vincent,M., Samani,N.J., Hilbert,P., Kaiser,M.A.,
            Beressi,J.P., Kotelevtsev,Y., Beckmann,J.S., Soubrier,F.,
            Sassard,J. et al.
  TITLE     Genetic determinants of diastolic and pulse pressure map to
            different loci in Lyon hypertensive rats
  JOURNAL   Nat Genet 3 (4), 354-357 (1993)
   PUBMED   7981757
REFERENCE   8  (bases 1 to 1404)
  AUTHORS   Yamamoto,K.K., Pousette,A., Chow,P., Wilson,H., el Shami,S. and
            French,C.K.
  TITLE     Isolation of a cDNA encoding a human serum marker for acute
            pancreatitis. Identification of pancreas-specific protein as
            pancreatic procarboxypeptidase B
  JOURNAL   J Biol Chem 267 (4), 2575-2581 (1992)
   PUBMED   1370825
REFERENCE   9  (bases 1 to 1404)
  AUTHORS   Kodama,H., Shimojo,N. and Suzuki,K.T.
  TITLE     Distribution of manganese in rat pancreas and identification of its
            primary binding protein as pro-carboxypeptidase B
  JOURNAL   Biochem J 278 (Pt 3) (Pt 3), 857-862 (1991)
   PUBMED   1898371
REFERENCE   10 (bases 1 to 1404)
  AUTHORS   Clauser,E., Gardell,S.J., Craik,C.S., MacDonald,R.J. and
            Rutter,W.J.
  TITLE     Structural characterization of the rat carboxypeptidase A1 and B
            genes. Comparative analysis of the rat carboxypeptidase gene family
  JOURNAL   J Biol Chem 263 (33), 17837-17845 (1988)
   PUBMED   3182872
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1, CF249065.1, CF249171.1 and AW916086.1.
            
            On Apr 12, 2016 this sequence version replaced NM_012533.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.612944.1,
                                           SRR26360172.162930.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                JAXUCZ010000002.1  104714593-104714662 c
            71-691              CF249065.1         1-621
            692-721             CF249171.1         651-680
            722-851             AW916086.1         80-209
            852-1121            JAXUCZ010000002.1  104684275-104684544 c
            1122-1403           AW916086.1         211-492
            1404-1673           JAXUCZ010000002.1  104684275-104684544 c
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q24"
     gene            1..1404
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /note="carboxypeptidase B1"
                     /db_xref="GeneID:24271"
                     /db_xref="RGD:2391"
     exon            1..88
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..1271
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /EC_number="3.4.17.2"
                     /note="pancreatic carboxypeptidase B1; carboxypeptidase B1
                     (tissue)"
                     /codon_start=1
                     /product="carboxypeptidase B precursor"
                     /protein_id="NP_036665.2"
                     /db_xref="GeneID:24271"
                     /db_xref="RGD:2391"
                     /translation="MLLLLALVSVALAHASEEHFDGNRVYRVSVHGEDHVNLIQELAN
                     TKEIDFWKPDSATQVKPLTTVDFHVKAEDVADVENFLEENEVHYEVLISNVRNALESQ
                     FDSHTRASGHSYTKYNKWETIEAWIQQVATDNPDLVTQSVIGTTFEGRNMYVLKIGKT
                     RPNKPAIFIDCGFHAREWISPAFCQWFVREAVRTYNQEIHMKQLLDELDFYVLPVVNI
                     DGYVYTWTKDRMWRKTRSTMAGSSCLGVDPNRNFNAGWCEVGASRSPCSETYCGPAPE
                     SEKETKALADFIRNNLSTIKAYLTIHSYSQMMLYPYSYDYKLPENYEELNALVKGAAK
                     ELATLHGTKYTYGPGATTIYPAAGGSDDWSYDQGIKYSFTFELRDTGFFGFLLPESQI
                     RQTCEETMLAVKYIANYVREHLY"
     exon            89..164
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            165..289
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            290..389
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            390..491
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            492..593
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            594..704
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            705..795
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            796..998
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1083
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1404
                     /gene="Cpb1"
                     /gene_synonym="Cpb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcccagtagt tcaggcaggc accatgttgc tgctactggc cctggtgagt gtggccttgg
       61 ctcatgcttc cgaggagcac tttgatggca accgggtgta ccgtgtcagt gtacatggtg
      121 aagatcacgt caacttaatt caggagctag ccaacaccaa agagattgat ttctggaaac
      181 cagattctgc tacacaagtg aagcctctca ctacagttga ctttcatgtt aaagcagaag
      241 atgttgctga tgtggagaac tttctggagg agaatgaagt tcactatgag gtactgataa
      301 gcaacgtgag aaatgctctg gaatcccagt ttgatagcca cacccgtgca agtggacaca
      361 gctacaccaa gtacaacaag tgggaaacga ttgaggcgtg gattcaacaa gttgccactg
      421 ataatccaga ccttgtcact cagagcgtca ttggaaccac atttgaagga cgtaacatgt
      481 atgtcctcaa gattggcaaa actagaccga ataagcctgc catcttcatc gattgtggtt
      541 tccatgcaag agagtggatt tctcctgcat tctgtcagtg gtttgtgaga gaggctgtcc
      601 gtacctataa tcaagagatc cacatgaaac agcttctaga tgaactggat ttctatgttc
      661 tgcctgtggt caacattgat ggctatgtct acacctggac taaggacaga atgtggagaa
      721 aaacccgctc tactatggct ggaagttcct gcttgggtgt agaccccaac aggaatttta
      781 atgctggctg gtgtgaagtg ggagcttctc ggagtccctg ctctgaaact tactgtggac
      841 cagccccaga gtctgaaaaa gagacaaagg ccctggcaga tttcatccgc aacaacctct
      901 ccaccatcaa ggcctacctg accatccact catactcaca gatgatgctc tacccttact
      961 cctatgacta caaactgcct gagaactatg aggaattgaa tgccctggtg aaaggtgcgg
     1021 caaaggagct tgccactctg catggcacca agtacacata tggcccagga gctacaacaa
     1081 tctatcctgc tgctggggga tctgacgact ggtcttatga tcagggaatc aaatattcat
     1141 ttacctttga actccgggat acaggcttct ttggctttct ccttcctgag tctcagatcc
     1201 gccagacctg tgaggagaca atgcttgcag tcaagtacat tgccaattat gtccgagaac
     1261 atctatatta gtgaagaatc tggaccacct tattccaaaa tgtttactgc tcatgtcttg
     1321 tttctctttc tcttaatttt ttgggtgtgt tgaccaattg gacctctaaa aatatcttaa
     1381 taaaatcata tcattgttgg aaaa
//
