LOCUS       NM_001013083            1306 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus carboxypeptidase A2 (Cpa2), mRNA.
ACCESSION   NM_001013083 XM_216107
VERSION     NM_001013083.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Pereira,H.J., Souza,L.L., Costa-Neto,C.M., Salgado,M.C. and
            Oliveira,E.B.
  TITLE     Carboxypeptidases A1 and A2 from the perfusate of rat mesenteric
            arterial bed differentially process angiotensin peptides
  JOURNAL   Peptides 33 (1), 67-76 (2012)
   PUBMED   22178042
  REMARK    GeneRIF: gene transcripts for CPA2 in mesentery and other
            extrapancreatic tissues indicate that CPA2 might play a role in the
            renin-angiotensin system in addition to it'sfunction as digestive
            enzyme
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Kwitek,A.E., Gullings-Handley,J., Yu,J., Carlos,D.C., Orlebeke,K.,
            Nie,J., Eckert,J., Lemke,A., Andrae,J.W., Bromberg,S., Pasko,D.,
            Chen,D., Scheetz,T.E., Casavant,T.L., Soares,M.B., Sheffield,V.C.,
            Tonellato,P.J. and Jacob,H.J.
  TITLE     High-density rat radiation hybrid maps containing over 24,000
            SSLPs, genes, and ESTs provide a direct link to the rat genome
            sequence
  JOURNAL   Genome Res 14 (4), 750-757 (2004)
   PUBMED   15060019
REFERENCE   3  (bases 1 to 1306)
  AUTHORS   Normant,E., Gros,C. and Schwartz,J.C.
  TITLE     Carboxypeptidase A isoforms produced by distinct genes or
            alternative splicing in brain and other extrapancreatic tissues
  JOURNAL   J Biol Chem 270 (35), 20543-20549 (1995)
   PUBMED   7657630
REFERENCE   4  (bases 1 to 1306)
  AUTHORS   Oppezzo,O., Ventura,S., Bergman,T., Vendrell,J., Jornvall,H. and
            Aviles,F.X.
  TITLE     Procarboxypeptidase in rat pancreas. Overall characterization and
            comparison of the activation processes
  JOURNAL   Eur J Biochem 222 (1), 55-63 (1994)
   PUBMED   8200353
REFERENCE   5  (bases 1 to 1306)
  AUTHORS   Faming,Z., Kobe,B., Stewart,C.B., Rutter,W.J. and Goldsmith,E.J.
  TITLE     Structural evolution of an enzyme specificity. The structure of rat
            carboxypeptidase A2 at 1.9-A resolution
  JOURNAL   J Biol Chem 266 (36), 24606-24612 (1991)
   PUBMED   1761558
REFERENCE   6  (bases 1 to 1306)
  AUTHORS   Pascual,R., Burgos,F.J., Salva,M., Soriano,F., Mendez,E. and
            Aviles,F.X.
  TITLE     Purification and properties of five different forms of human
            procarboxypeptidases
  JOURNAL   Eur J Biochem 179 (3), 609-616 (1989)
   PUBMED   2920728
REFERENCE   7  (bases 1 to 1306)
  AUTHORS   Clauser,E., Gardell,S.J., Craik,C.S., MacDonald,R.J. and
            Rutter,W.J.
  TITLE     Structural characterization of the rat carboxypeptidase A1 and B
            genes. Comparative analysis of the rat carboxypeptidase gene family
  JOURNAL   J Biol Chem 263 (33), 17837-17845 (1988)
   PUBMED   3182872
REFERENCE   8  (bases 1 to 1306)
  AUTHORS   Gardell,S.J., Craik,C.S., Clauser,E., Goldsmith,E.J., Stewart,C.B.,
            Graf,M. and Rutter,W.J.
  TITLE     A novel rat carboxypeptidase, CPA2: characterization, molecular
            cloning, and evolutionary implications on substrate specificity in
            the carboxypeptidase gene family
  JOURNAL   J Biol Chem 263 (33), 17828-17836 (1988)
   PUBMED   3182871
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF249075.1, CF249031.1, CF249511.1 and CF249156.1.
            
            On Feb 5, 2008 this sequence version replaced NM_001013083.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5756307,
                              SAMEA5760383 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-605               CF249075.1         1-605
            606-901             CF249031.1         292-587
            902-903             CF249511.1         266-267
            904-1306            CF249156.1         1-403
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q22"
     gene            1..1306
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /note="carboxypeptidase A2"
                     /db_xref="GeneID:296959"
                     /db_xref="RGD:1305563"
     exon            1..69
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     CDS             5..1258
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /EC_number="3.4.17.15"
                     /note="carboxypeptidase A2 (pancreatic)"
                     /codon_start=1
                     /product="carboxypeptidase A2 precursor"
                     /protein_id="NP_001013101.2"
                     /db_xref="GeneID:296959"
                     /db_xref="RGD:1305563"
                     /translation="MRLTLLLAALLGYIYCQETFVGDQVLEIIPSHEEQIRTLLQLEA
                     EEHLELDFWKSPTIPGETVHVRVPFASIQAVKVFLESQGIDYSIMIEDVQVLLDQERE
                     EMLFNQQRERGGNFNFEAYHTLEEIYQEMDNLVAENPGLVSKVNIGSSFENRPMNVLK
                     FSTGGDKPAIWLDAGIHAREWVTQATALWTANKIASDYGTDPAITSLLNTLDIFLLPV
                     TNPDGYVFSQTTNRMWRKTRSKRSGSGCVGVDPNRNWDANFGGPGASSSPCSDSYHGP
                     KPNSEVEVKSIVDFIKSHGKVKAFITLHSYSQLLMFPYGYKCTKPDDFNELDEVAQKA
                     AQALKRLHGTSYKVGPICSVIYQASGGSIDWAYDLGIKYSFAFELRDTGFYGFLLPAK
                     QILPTAEETWLGLKTIMEHVRDHPY"
     exon            70..151
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            152..286
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            287..382
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            383..484
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            485..583
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            584..694
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            695..785
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            786..985
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1070
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1306
                     /gene="Cpa2"
                     /gene_synonym="CPA2(S)"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaccatgagg ttgaccctgt tattggctgc cctacttggg tatatctact gtcaagaaac
       61 gtttgtggga gatcaagttc ttgagatcat cccaagtcat gaagagcaaa ttagaactct
      121 gctgcaattg gaggctgaag agcatctcga gcttgatttt tggaaatcac ccaccatccc
      181 tggggagaca gtccacgttc gagtcccttt tgctagcatc caggctgtca aggttttctt
      241 ggagtcccag gggatcgatt attccatcat gatcgaagat gttcaagttc tgttggatca
      301 agagcgggaa gaaatgctat tcaaccagca aagagagcgt ggtgggaact tcaactttga
      361 ggcctaccat accctggaag agatttacca agaaatggat aacctcgtgg ctgagaaccc
      421 cggtctagtg agcaaagtga atatcggttc atcttttgaa aatcggccca tgaatgtgct
      481 caagttcagc accggaggag acaagccagc catctggctt gatgcgggga tccatgctcg
      541 agagtgggtt acacaagcta ctgcactgtg gacagcaaac aagattgctt ctgattatgg
      601 aactgatcca gccatcacgt ctcttcttaa tactctggat atcttcctgc tgcctgtcac
      661 caatcccgat ggatatgtgt tctcccaaac caccaatcga atgtggagaa agacccgttc
      721 taaaaggtca ggaagcggct gtgtcggtgt ggatcctaat cggaactggg atgcaaattt
      781 cggaggacct ggagccagta gcagcccttg ctctgactca taccacggac ccaagcccaa
      841 ctctgaagtt gaagtgaaat ccatagtgga cttcatcaag agccacggga aagtcaaagc
      901 ttttattacc cttcacagct attcccaact gcttatgttc ccctatggct ataaatgtac
      961 caagccagat gactttaatg agctggatga agtggcccag aaggctgccc aggctttgaa
     1021 aagactgcac ggcaccagtt ataaagtggg acccatctgt tctgtcatct accaggcgag
     1081 tggtggaagc atcgactggg cttacgacct tggcatcaaa tactcatttg cctttgaact
     1141 gagggacaca ggtttctatg gcttcctcct gccagccaag cagatcttgc cgacagcaga
     1201 agagacctgg cttggcctga agaccatcat ggagcacgta cgagaccacc cctattaggg
     1261 atcggcagaa tagaccgaca ccattaaagt ctctgtggtc taaagc
//
