LOCUS       NM_016998               1633 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus carboxypeptidase A1 (Cpa1), mRNA.
ACCESSION   NM_016998
VERSION     NM_016998.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Pereira,H.J., Souza,L.L., Costa-Neto,C.M., Salgado,M.C. and
            Oliveira,E.B.
  TITLE     Carboxypeptidases A1 and A2 from the perfusate of rat mesenteric
            arterial bed differentially process angiotensin peptides
  JOURNAL   Peptides 33 (1), 67-76 (2012)
   PUBMED   22178042
  REMARK    GeneRIF: gene transcripts for CPA1 in mesentery and other
            extrapancreatic tissues indicate that CPA1 might play a role in the
            renin-angiotensin system in addition to it'sfunction as digestive
            enzyme
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Kuhlein,H.N., Tegeder,I., Moser,C., Lim,H.Y., Haussler,A.,
            Spieth,K., Jennes,I., Marschalek,R., Beckhaus,T., Karas,M.,
            Fauth,M., Ehnert,C., Geisslinger,G. and Niederberger,E.
  TITLE     Nerve injury evoked loss of latexin expression in spinal cord
            neurons contributes to the development of neuropathic pain
  JOURNAL   PLoS One 6 (4), e19270 (2011)
   PUBMED   21572518
  REMARK    GeneRIF: Data show that latexin protein expression was reduced
            after SNI, and the decrease of latexin was associated with an
            increase of the activity of carboxypeptidase A.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1633)
  AUTHORS   Wang,S.F., Liang,Q., Li,G.W. and Gao,K.
  TITLE     Gene expression profile in rat small intestinal allografts after
            cold preservation/reperfusion
  JOURNAL   World J Gastroenterol 11 (6), 885-889 (2005)
   PUBMED   15682487
REFERENCE   4  (bases 1 to 1633)
  AUTHORS   Eriyamremu,G.E., Asagba,S.O., Onyeneke,E.C. and Adaikpoh,M.A.
  TITLE     Changes in carboxypeptidase A, dipeptidase and Na+/K+ ATPase
            activities in the intestine of rats orally exposed to different
            doses of cadmium
  JOURNAL   Biometals 18 (1), 1-6 (2005)
   PUBMED   15865404
  REMARK    GeneRIF: carboxypeptidase A, dipeptidase and Na+/K+ ATPase levels
            in the rat intestine change when exposed to different doses of
            cadmium
REFERENCE   5  (bases 1 to 1633)
  AUTHORS   Pascual,R., Burgos,F.J., Salva,M., Soriano,F., Mendez,E. and
            Aviles,F.X.
  TITLE     Purification and properties of five different forms of human
            procarboxypeptidases
  JOURNAL   Eur J Biochem 179 (3), 609-616 (1989)
   PUBMED   2920728
REFERENCE   6  (bases 1 to 1633)
  AUTHORS   Clauser,E., Gardell,S.J., Craik,C.S., MacDonald,R.J. and
            Rutter,W.J.
  TITLE     Structural characterization of the rat carboxypeptidase A1 and B
            genes. Comparative analysis of the rat carboxypeptidase gene family
  JOURNAL   J Biol Chem 263 (33), 17837-17845 (1988)
   PUBMED   3182872
REFERENCE   7  (bases 1 to 1633)
  AUTHORS   Quinto,C., Quiroga,M., Swain,W.F., Nikovits,W.C. Jr.,
            Standring,D.N., Pictet,R.L., Valenzuela,P. and Rutter,W.J.
  TITLE     Rat preprocarboxypeptidase A: cDNA sequence and preliminary
            characterization of the gene
  JOURNAL   Proc Natl Acad Sci U S A 79 (1), 31-35 (1982)
   PUBMED   6275388
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On May 14, 2011 this sequence version replaced NM_016998.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J00713.1, FQ232505.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06621351 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-373               JAXUCZ010000004.1  60224801-60225173
            374-455             JAXUCZ010000004.1  60225595-60225676
            456-689             JAXUCZ010000004.1  60226119-60226352
            690-791             JAXUCZ010000004.1  60226499-60226600
            792-893             JAXUCZ010000004.1  60227104-60227205
            894-1004            JAXUCZ010000004.1  60227376-60227486
            1005-1095           JAXUCZ010000004.1  60227884-60227974
            1096-1295           JAXUCZ010000004.1  60228425-60228624
            1296-1380           JAXUCZ010000004.1  60229124-60229208
            1381-1633           JAXUCZ010000004.1  60230676-60230928
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q22"
     gene            1..1633
                     /gene="Cpa1"
                     /note="carboxypeptidase A1"
                     /db_xref="GeneID:24269"
                     /db_xref="RGD:2388"
     exon            1..373
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     CDS             309..1568
                     /gene="Cpa1"
                     /EC_number="3.4.17.1"
                     /note="carboxypeptidase A1 (pancreatic)"
                     /codon_start=1
                     /product="carboxypeptidase A1 preproprotein"
                     /protein_id="NP_058694.2"
                     /db_xref="GeneID:24269"
                     /db_xref="RGD:2388"
                     /translation="MKRLLILSLLLEAVCGNENFVGHQVLRISAADEAQVQKVKELED
                     LEHLQLDFWRDAARAGIPIDVRVPFPSIQSVKAFLEYHGISYEIMIEDVQLLLDEEKQ
                     QMSAFQARALSTDSFNYATYHTLDEIYEFMDLLVAEHPQLVSKIQIGNTFEGRPIHVL
                     KFSTGGTNRPAIWIDTGIHSREWVTQASGVWFAKKITKDYGQDPTFTAVLDNMDIFLE
                     IVTNPDGFAYTHKTNRMWRKTRSHTQGSLCVGVDPNRNWDAGFGMAGASSNPCSETYR
                     GKFPNSEVEVKSIVDFVTSHGNIKAFISIHSYSQLLLYPYGYTSEPAPDQAELDQLAK
                     SAVTALTSLHGTKFKYGSIIDTIYQASGSTIDWTYSQGIKYSFTFELRDTGLRGFLLP
                     ASQIIPTAEETWLALLTIMDHTVKHPY"
     exon            374..455
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     exon            456..689
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     exon            690..791
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     exon            792..893
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1004
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1095
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1295
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1380
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1633
                     /gene="Cpa1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgtccttgtt tctgacatcg tgagctaaca ataaggtatc tgcggctgtg ggaaaccgga
       61 tgagtagtcc cagctgggag acctcaggcg cagctgaccc catggtcaag ggtagaagcc
      121 tggcttatct ctccacctgc cttgttcccc gatactttat caggaagagt ggaaaggtgc
      181 cgcagtttgg aactccagcc tctccctccc catgggactt gatccagatg tgaggggaac
      241 tgtccccggg gacccctgtc agcgtttaaa aaggcctcag atctcagtct tggcctgacc
      301 ttctcaccat gaagagacta ctgattctga gtctgctgct ggaagcagtc tgtggcaatg
      361 agaactttgt gggacaccag gttctccgaa tctctgcagc cgatgaagcc caggtgcaga
      421 aagtgaagga gttggaggac ctggagcact tgcagttgga cttctggcgg gacgctgccc
      481 gggccggtat ccccattgat gtcagagtgc ccttccccag catccaatct gtgaaagcat
      541 tcttggaata tcatggtatt agctatgaga tcatgattga agatgtgcag ttactgctcg
      601 atgaggagaa acagcagatg tctgccttcc aggccagggc cttgtccact gactctttca
      661 attatgccac ctatcatacg ctggatgaga tctacgaatt catggacctg ctggtggctg
      721 agcacccaca gcttgtgagc aagatccaga tcggcaacac ctttgaaggt cgccccatcc
      781 atgtgctgaa gttcagcact ggagggacca atcgcccagc aatctggatc gacactggca
      841 tccattctag ggagtgggtc acccaggcta gtggggtctg gtttgcaaag aagatcacca
      901 aagactatgg ccaggacccc accttcacag ccgttcttga caacatggac atcttcctgg
      961 agattgtcac caaccctgat ggttttgcct acacccacaa aacgaatcgc atgtggcgca
     1021 agactcgatc acacacccag ggctccctct gtgttggtgt ggaccccaac aggaactggg
     1081 acgctggctt tgggatggcc ggagcaagta gcaacccctg ctcggaaact taccgaggca
     1141 aatttcccaa ctctgaggtg gaggtcaagt ccatcgtgga ctttgtgacg agccatggga
     1201 acatcaaggc cttcatctcc atccacagct attcccagct cctgctctac ccctacggct
     1261 acacgtcgga accagcccct gaccaggcag agctggatca gctagctaag tctgctgtga
     1321 cagccttgac gtctctacac gggaccaagt tcaagtatgg cagcatcatc gatacaatct
     1381 atcaagccag tgggagcact atcgattgga cctacagcca gggcatcaag tactctttca
     1441 cttttgaact gagggacact gggcttagag gattcctgct gcctgcctcc cagatcatcc
     1501 ctacggcgga ggagacatgg ctggcccttt tgaccatcat ggaccacaca gtcaaacacc
     1561 cctactgaaa gcagccctta tcccccacct ctttcttctc cttagtccca ataaagtctg
     1621 attgtataag agg
//
