LOCUS       NM_019274               2737 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus collagen like tail subunit of asymmetric
            acetylcholinesterase (Colq), mRNA.
ACCESSION   NM_019274
VERSION     NM_019274.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Choi,R.C., Chen,V.P., Luk,W.K., Yung,A.W., Ng,A.H., Dong,T.T. and
            Tsim,K.W.
  TITLE     Expression of cAMP-responsive element binding proteins (CREBs) in
            fast- and slow-twitch muscles: a signaling pathway to account for
            the synaptic expression of collagen-tailed subunit (ColQ) of
            acetylcholinesterase at the rat neuromuscular junction
  JOURNAL   Chem Biol Interact 203 (1), 282-286 (2013)
   PUBMED   23159887
  REMARK    GeneRIF: differential distribution of P-CREB in fast-and
            slow-twitch muscles might support the crucial role of
            cAMP-dependent signaling in controlling the synapse-specific
            expression of ColQ-1a in fast-twitch muscles
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Glisovic,S., Pregelj,P., Dolenc,I. and Sketelj,J.
  TITLE     Suppression of collagen Q expression in the extrajunctional regions
            of rat fast muscles is encoded in their stem cells (satellite
            cells)
  JOURNAL   Chem Biol Interact 203 (1), 292-296 (2013)
   PUBMED   22944068
  REMARK    GeneRIF: Extensive neural suppression of the extrajunctional ColQ
            expression in regenerating fast muscles during maturation is a very
            slow process requiring 30-60 days.
REFERENCE   3  (bases 1 to 2737)
  AUTHORS   Glisovic,S., Trinkaus,M., Pregelj,P. and Sketelj,J.
  TITLE     The asymmetric molecular forms of AChE and the expression of
            collagen Q in mature and immature fast and slow rat muscles
  JOURNAL   Chem Biol Interact 187 (1-3), 90-95 (2010)
   PUBMED   20188715
  REMARK    GeneRIF: extrajunctional levels of ColQ mRNA in non-innervated
            regenerating fast and slow muscles
REFERENCE   4  (bases 1 to 2737)
  AUTHORS   Sigoillot,S.M., Bourgeois,F. and Legay,C.
  TITLE     Cholinesterases regulation in the absence of ColQ
  JOURNAL   Chem Biol Interact 187 (1-3), 84-89 (2010)
   PUBMED   20153305
  REMARK    GeneRIF: AChE, BChE and PRiMA mRNA level modifications found in the
            absence of ColQ cannot compensate for the physiological defects
            observed at the ColQ-deficient neuromuscular junction.
REFERENCE   5  (bases 1 to 2737)
  AUTHORS   Sigoillot,S.M., Bourgeois,F., Lambergeon,M., Strochlic,L. and
            Legay,C.
  TITLE     ColQ controls postsynaptic differentiation at the neuromuscular
            junction
  JOURNAL   J Neurosci 30 (1), 13-23 (2010)
   PUBMED   20053883
  REMARK    GeneRIF: In addition to its structural role, ColQ has important
            regulatory functions at the synapse by controlling acetylcholine
            receptor clustering through its interaction with MuSK.
REFERENCE   6  (bases 1 to 2737)
  AUTHORS   Trinkaus,M., Pregelj,P., Trkov,S. and Sketelj,J.
  TITLE     Neural regulation of acetylcholinesterase-associated collagen Q in
            rat skeletal muscles
  JOURNAL   J Neurochem 105 (6), 2535-2544 (2008)
   PUBMED   18373559
  REMARK    GeneRIF: Extrajunctional collagen Q expression in fast muscles is
            independent of muscle activation and is partly nerve-dependent.
REFERENCE   7  (bases 1 to 2737)
  AUTHORS   Deprez,P., Inestrosa,N.C. and Krejci,E.
  TITLE     Two different heparin-binding domains in the triple-helical domain
            of ColQ, the collagen tail subunit of synaptic acetylcholinesterase
  JOURNAL   J Biol Chem 278 (26), 23233-23242 (2003)
   PUBMED   12684510
  REMARK    GeneRIF: Two heparin-binding domains with different properties that
            may have non-redundant functions are described; may coordinate
            acetylcholinesterase positioning within the the neuromuscular
            junction basal lamina.
REFERENCE   8  (bases 1 to 2737)
  AUTHORS   Feng,G., Krejci,E., Molgo,J., Cunningham,J.M., Massoulie,J. and
            Sanes,J.R.
  TITLE     Genetic analysis of collagen Q: roles in acetylcholinesterase and
            butyrylcholinesterase assembly and in synaptic structure and
            function
  JOURNAL   J Cell Biol 144 (6), 1349-1360 (1999)
   PUBMED   10087275
REFERENCE   9  (bases 1 to 2737)
  AUTHORS   Ohno,K., Brengman,J., Tsujino,A. and Engel,A.G.
  TITLE     Human endplate acetylcholinesterase deficiency caused by mutations
            in the collagen-like tail subunit (ColQ) of the asymmetric enzyme
  JOURNAL   Proc Natl Acad Sci U S A 95 (16), 9654-9659 (1998)
   PUBMED   9689136
REFERENCE   10 (bases 1 to 2737)
  AUTHORS   Krejci,E., Thomine,S., Boschetti,N., Legay,C., Sketelj,J. and
            Massoulie,J.
  TITLE     The mammalian gene of acetylcholinesterase-associated collagen
  JOURNAL   J Biol Chem 272 (36), 22840-22847 (1997)
   PUBMED   9278446
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000016.1.
            
            On Feb 26, 2021 this sequence version replaced NM_019274.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF007583.1, SRR26643289.37742.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMN06621351
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-151               JAXUCZ010000016.1  6793351-6793501     c
            152-264             JAXUCZ010000016.1  6770381-6770493     c
            265-363             JAXUCZ010000016.1  6769529-6769627     c
            364-408             JAXUCZ010000016.1  6766301-6766345     c
            409-435             JAXUCZ010000016.1  6765938-6765964     c
            436-507             JAXUCZ010000016.1  6764280-6764351     c
            508-570             JAXUCZ010000016.1  6761839-6761901     c
            571-597             JAXUCZ010000016.1  6761451-6761477     c
            598-642             JAXUCZ010000016.1  6760949-6760993     c
            643-678             JAXUCZ010000016.1  6760751-6760786     c
            679-759             JAXUCZ010000016.1  6757293-6757373     c
            760-865             JAXUCZ010000016.1  6752978-6753083     c
            866-1005            JAXUCZ010000016.1  6746074-6746213     c
            1006-1125           JAXUCZ010000016.1  6745335-6745454     c
            1126-1246           JAXUCZ010000016.1  6744851-6744971     c
            1247-1349           JAXUCZ010000016.1  6743106-6743208     c
            1350-2737           JAXUCZ010000016.1  6738251-6739638     c
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p16"
     gene            1..2737
                     /gene="Colq"
                     /note="collagen like tail subunit of asymmetric
                     acetylcholinesterase"
                     /db_xref="GeneID:29755"
                     /db_xref="RGD:2377"
     exon            1..151
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..1419
                     /gene="Colq"
                     /note="acetylcholinesterase collagenic tail peptide; AChE
                     Q subunit; acetylcholinesterase-associated collagen;
                     collagen-like tail subunit (single strand of homotrimer)
                     of asymmetric acetylcholinesterase"
                     /codon_start=1
                     /product="acetylcholinesterase collagenic tail peptide
                     precursor"
                     /protein_id="NP_062147.2"
                     /db_xref="GeneID:29755"
                     /db_xref="RGD:2377"
                     /translation="MAVLNPMTLGIYLQLFFCSIVSQPTFINSVLPISAALPGLDQKK
                     RGNHKACCLLMPPPPPLFPPPFFRGSRSPLLSPDMKNLLELEASPSPCMQGSLGSPGP
                     PGPQGPPGLPGKAGPKGEKGDLGRPGRKGRPGPPGVPGEPGPVGWPGPEGPRGEKGDV
                     GMMGLPGSRGPMGSKGFPGSRGEKGSRGERGDLGPKGEKGFPGFPGMLGQKGEMGPKG
                     ESGIAGHRGPTGRPGKRGKQGQKGDSGIMGPPGKPGPSGQPGRQGPPGPPGPPSAGQL
                     VMGLKGERGFPGPPGRCLCGPPANVNNPSYGDPMYGRGSPRVPAIFVVNNQEELEKLN
                     TQNAIAFRRDQRSLYFKDSLGWLPIQLTPFYPVGYTIKQHGTCGDGVLQPGEECDDGN
                     PDVSDGCIDCHRAYCGDGYRHRGVEDCDGSDFGYLTCETYLPGSYGELRCTQYCSIDS
                     TPCRYFT"
     exon            152..264
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            265..363
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            364..408
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            409..435
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            436..507
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            508..570
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            571..597
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            598..642
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            643..678
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            679..759
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            760..865
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1005
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1125
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1246
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1349
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..2737
                     /gene="Colq"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcagcctt gccctctgac ttgatattta ccagacccgg ccagcatggc tgtcctgaat
       61 ccaatgactt tgggaattta tctccaactc ttcttctgct ccatcgtgtc gcagccaact
      121 ttcatcaaca gtgtcctccc aatctcagca gcccttcctg gcctggatca gaagaaacga
      181 ggcaaccaca aagcatgctg cctactgatg cccccgccac ccccactctt cccaccgcca
      241 ttcttcagag gcagccgaag cccgcttctc tccccagaca tgaagaatct tctggaactt
      301 gaggcctccc catcaccctg catgcaaggc tctctaggct ctcctggccc ccctgggccc
      361 cagggtccac ctgggcttcc tggcaaggca ggaccaaagg gagaaaaggg ggatcttggc
      421 cgcccaggaa ggaagggtag acctggaccc ccaggtgttc ctggcgagcc cgggcccgtt
      481 ggctggccag gccctgaagg acccaggggc gaaaaaggcg acgtgggtat gatgggtctg
      541 ccagggtcaa gaggaccgat gggctctaag ggcttccctg gatccagagg ggaaaaggga
      601 tccagaggtg aaagagggga cttgggtccc aaaggagaaa agggtttccc tggatttcct
      661 ggaatgctgg ggcagaaagg tgaaatgggt ccaaagggag aatctgggat agcaggacat
      721 cggggcccca caggaagacc agggaaacga ggcaagcagg gtcagaaggg agacagtgga
      781 attatgggcc caccaggcaa acctgggcct tctggtcaac ctggccgtca aggtcctcct
      841 gggcccccag gacccccatc tgcaggacaa cttgtaatgg gactcaaagg ggaaagagga
      901 tttccagggc ccccgggaag atgcctttgc ggaccccctg cgaatgtgaa taacccctcc
      961 tatggggacc ccatgtatgg gcgcggctcc cctcgagttc ctgcgatttt tgtggtcaac
     1021 aaccaggagg agcttgagaa gctgaacact caaaatgcca ttgcctttcg aagagaccag
     1081 cgatctctgt acttcaaaga tagccttggc tggctcccca tccagctaac ccctttctac
     1141 ccagtgggtt acaccataaa gcagcatggc acctgtgggg atggggtcct gcagcctggg
     1201 gaggaatgtg acgatggtaa cccggatgtg agtgacggct gcattgactg tcatcgggcc
     1261 tactgtggag acggttatcg gcaccggggc gtggaggact gtgacggctc tgactttggc
     1321 tacctgacgt gcgagaccta tctccctgga tcatacggag aacttcggtg cacccagtat
     1381 tgctccatcg actccacacc ctgccgatac ttcacgtgag aggctgcagg gacaggtggg
     1441 ctgtggccca tggacccgtt ggctgtttct ctacccttca catcaagctg gccttgccct
     1501 ctcctgggac agtgcccttt gaccttcatg tgacaaaaca aggatatatt cttgctgcta
     1561 cgacatgctt ttgactccgt atgactggaa gttttggacc actgaatcct gtcttaattc
     1621 taagaaccag aatccttcta tacgcccacc ctggaaactt ccccatcccc tccaatctgg
     1681 ctaaacagct ggctgtaatg cctgatctct tctggaaaga tgctggctca agccgtggat
     1741 ttggcttagc actgttaagc atacaggcat ctacaggcct ggcgtggttc gttctggtct
     1801 catccaggtt taggggtgct cgtgaacagg cttctgcacc tccaagcaca tctgttccga
     1861 cccaaggcag ccagctgctg ggtctctcaa gatttaagaa ccacatcata ggaggtgaga
     1921 aattctcttg gtgcagctcc cagcttcaga cctgctgagg gggacccttc agctggctgg
     1981 agcctggatc aacttcatgt tgatcagtca ggggtacgag agagccacaa ggcccaagct
     2041 tggtcccagg aagagggtga aggctcagct gagtcactgc catgtgaccc ctaccagaac
     2101 agaaaccttc ttcctcccga agttctctga gggcaccatc tgaccttgtg cttctctctg
     2161 caccttgacc tctgtcttga cagtgccctc tgcaaccgtg tcctgtgtga aagtctcacc
     2221 cctcatgccc agcccgtgct gctgtttcct catgctggct tccctggctt gggctccact
     2281 gccaagtcct tggctgcagg acgcgccggt gactctcttc catgcccatc ttgcataccc
     2341 cttgttccct cactgtgtgc ccaacctctt cctttatcct gggattcttt ttcctaagcc
     2401 ctgcatttct gagaactgcc tttcccatat ttcatgaatg gggtttggct ctatttgggg
     2461 aaaacatcgc tttttgtccc cctccccaat gcgcgcacct gccttgcccc actacccagg
     2521 ctgcctctgg gctgtgcaaa gctcttcagt gagcccaagc cttgggaatt cagcaggtgg
     2581 caccttctca cccagcaagt ccgagctgtg aatggtattt cctgagagaa atcagcctga
     2641 ggctgtcttc atgggcagac agctatgcta tttactattc tgggacttct gggtgggcaa
     2701 acaagaaata aaggtgattc ttggctgtgg ctcagtc
//
