LOCUS       NM_001414896            5053 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus collagen type II alpha 1 chain (Col2a1),
            transcript variant 1, mRNA.
ACCESSION   NM_001414896 XM_006242308
VERSION     NM_001414896.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Zhang,X., Nham,G.T.H., Ito,K. and Shinomura,T.
  TITLE     Gene expression of type II collagen is regulated by direct
            interaction with Kruppel-like factor 4 and AT-rich interactive
            domain 5B
  JOURNAL   Gene 773, 145381 (2021)
   PUBMED   33383116
  REMARK    GeneRIF: Gene expression of type II collagen is regulated by direct
            interaction with Kruppel-like factor 4 and AT-rich interactive
            domain 5B.
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Korotkyi,O.H., Vovk,A.A., Dranitsina,A.S., Falalyeyeva,T.M.,
            Dvorshchenko,K.O., Fagoonee,S. and Ostapchenko,L.I.
  TITLE     The influence of probiotic diet and chondroitin sulfate
            administration on Ptgs2, Tgfb1 and Col2a1 expression in rat knee
            cartilage during monoiodoacetate-induced osteoarthritis
  JOURNAL   Minerva Med 110 (5), 419-424 (2019)
   PUBMED   30938133
  REMARK    GeneRIF: Induction of OA caused downregulation of Col2a1.
            Expression of Col2a1 showed upregulation in therapeutic group with
            separate administration chondroitin sulfate or a probiotic diet and
            the cumulative effects in case of co-administration.
REFERENCE   3  (bases 1 to 5053)
  AUTHORS   Nham,G.T.H., Zhang,X., Asou,Y. and Shinomura,T.
  TITLE     Expression of type II collagen and aggrecan genes is regulated
            through distinct epigenetic modifications of their multiple
            enhancer elements
  JOURNAL   Gene 704, 134-141 (2019)
   PUBMED   30981839
  REMARK    GeneRIF: E1 and E2 enhancer activities in type II collagen and
            aggrecan are regulated through distinct epigenetic modifications by
            histone deacetylase 10 and sirtuin 6.
REFERENCE   4  (bases 1 to 5053)
  AUTHORS   Flowers,S.A., Zieba,A., Ornros,J., Jin,C., Rolfson,O.,
            Bjorkman,L.I., Eisler,T., Kalamajski,S., Kamali-Moghaddam,M. and
            Karlsson,N.G.
  TITLE     Lubricin binds cartilage proteins, cartilage oligomeric matrix
            protein, fibronectin and collagen II at the cartilage surface
  JOURNAL   Sci Rep 7 (1), 13149 (2017)
   PUBMED   29030641
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5053)
  AUTHORS   Yasuda,H., Oh,C.D., Chen,D., de Crombrugghe,B. and Kim,J.H.
  TITLE     A Novel Regulatory Mechanism of Type II Collagen Expression via a
            SOX9-dependent Enhancer in Intron 6
  JOURNAL   J Biol Chem 292 (2), 528-538 (2017)
   PUBMED   27881681
  REMARK    GeneRIF: These findings suggest a novel mechanism of action of
            SOX5/6; namely, the SOX9/5/6 combination enhances Col2a1
            transcription through a novel enhancer in intron 6 together with
            the enhancer in intron 1.
REFERENCE   6  (bases 1 to 5053)
  AUTHORS   Bogaert,R., Tiller,G.E., Weis,M.A., Gruber,H.E., Rimoin,D.L.,
            Cohn,D.H. and Eyre,D.R.
  TITLE     An amino acid substitution (Gly853-->Glu) in the collagen alpha
            1(II) chain produces hypochondrogenesis
  JOURNAL   J Biol Chem 267 (31), 22522-22526 (1992)
   PUBMED   1429602
REFERENCE   7  (bases 1 to 5053)
  AUTHORS   Ahmad,N.N., Ala-Kokko,L., Knowlton,R.G., Jimenez,S.A., Weaver,E.J.,
            Maguire,J.I., Tasman,W. and Prockop,D.J.
  TITLE     Stop codon in the procollagen II gene (COL2A1) in a family with the
            Stickler syndrome (arthro-ophthalmopathy)
  JOURNAL   Proc Natl Acad Sci U S A 88 (15), 6624-6627 (1991)
   PUBMED   1677770
REFERENCE   8  (bases 1 to 5053)
  AUTHORS   Wood,A., Ashhurst,D.E., Corbett,A. and Thorogood,P.
  TITLE     The transient expression of type II collagen at tissue interfaces
            during mammalian craniofacial development
  JOURNAL   Development 111 (4), 955-968 (1991)
   PUBMED   1879364
REFERENCE   9  (bases 1 to 5053)
  AUTHORS   Seegmiller,R.E., Brown,K. and Chandrasekhar,S.
  TITLE     Histochemical, immunofluorescence, and ultrastructural differences
            in fetal cartilage among three genetically distinct
            chondrodystrophic mice
  JOURNAL   Teratology 38 (6), 579-592 (1988)
   PUBMED   3070812
REFERENCE   10 (bases 1 to 5053)
  AUTHORS   Kohno,K., Sullivan,M. and Yamada,Y.
  TITLE     Structure of the promoter of the rat type II procollagen gene
  JOURNAL   J Biol Chem 260 (7), 4441-4447 (1985)
   PUBMED   2984204
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Dec 14, 2022 this sequence version replaced XM_006242308.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN09345238,
                              SAMN09345329 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-240               JAXUCZ010000007.1  131006388-131006627 c
            241-444             JAXUCZ010000007.1  131002464-131002667 c
            445-461             JAXUCZ010000007.1  131001101-131001117 c
            462-494             JAXUCZ010000007.1  131000683-131000715 c
            495-527             JAXUCZ010000007.1  131000519-131000551 c
            528-581             JAXUCZ010000007.1  131000365-131000418 c
            582-686             JAXUCZ010000007.1  131000128-131000232 c
            687-764             JAXUCZ010000007.1  130999164-130999241 c
            765-809             JAXUCZ010000007.1  130998499-130998543 c
            810-863             JAXUCZ010000007.1  130998340-130998393 c
            864-917             JAXUCZ010000007.1  130997922-130997975 c
            918-971             JAXUCZ010000007.1  130997174-130997227 c
            972-1025            JAXUCZ010000007.1  130996813-130996866 c
            1026-1079           JAXUCZ010000007.1  130996633-130996686 c
            1080-1124           JAXUCZ010000007.1  130996315-130996359 c
            1125-1178           JAXUCZ010000007.1  130995801-130995854 c
            1179-1223           JAXUCZ010000007.1  130992960-130993004 c
            1224-1277           JAXUCZ010000007.1  130992523-130992576 c
            1278-1376           JAXUCZ010000007.1  130991058-130991156 c
            1377-1421           JAXUCZ010000007.1  130990768-130990812 c
            1422-1520           JAXUCZ010000007.1  130990578-130990676 c
            1521-1574           JAXUCZ010000007.1  130990357-130990410 c
            1575-1682           JAXUCZ010000007.1  130989905-130990012 c
            1683-1736           JAXUCZ010000007.1  130989493-130989546 c
            1737-1835           JAXUCZ010000007.1  130989310-130989408 c
            1836-1889           JAXUCZ010000007.1  130989130-130989183 c
            1890-1988           JAXUCZ010000007.1  130988532-130988630 c
            1989-2042           JAXUCZ010000007.1  130988129-130988182 c
            2043-2096           JAXUCZ010000007.1  130987689-130987742 c
            2097-2150           JAXUCZ010000007.1  130987355-130987408 c
            2151-2204           JAXUCZ010000007.1  130987095-130987148 c
            2205-2249           JAXUCZ010000007.1  130986833-130986877 c
            2250-2348           JAXUCZ010000007.1  130986590-130986688 c
            2349-2456           JAXUCZ010000007.1  130986212-130986319 c
            2457-2510           JAXUCZ010000007.1  130985797-130985850 c
            2511-2564           JAXUCZ010000007.1  130985480-130985533 c
            2565-2618           JAXUCZ010000007.1  130985065-130985118 c
            2619-2672           JAXUCZ010000007.1  130984710-130984763 c
            2673-2780           JAXUCZ010000007.1  130984319-130984426 c
            2781-2834           JAXUCZ010000007.1  130983782-130983835 c
            2835-2888           JAXUCZ010000007.1  130983314-130983367 c
            2889-3050           JAXUCZ010000007.1  130982475-130982636 c
            3051-3158           JAXUCZ010000007.1  130982195-130982302 c
            3159-3266           JAXUCZ010000007.1  130981864-130981971 c
            3267-3320           JAXUCZ010000007.1  130981596-130981649 c
            3321-3428           JAXUCZ010000007.1  130981348-130981455 c
            3429-3482           JAXUCZ010000007.1  130981110-130981163 c
            3483-3590           JAXUCZ010000007.1  130980890-130980997 c
            3591-3644           JAXUCZ010000007.1  130980598-130980651 c
            3645-3752           JAXUCZ010000007.1  130980080-130980187 c
            3753-4041           JAXUCZ010000007.1  130979490-130979778 c
            4042-4229           JAXUCZ010000007.1  130979126-130979313 c
            4230-4472           JAXUCZ010000007.1  130978588-130978830 c
            4473-5053           JAXUCZ010000007.1  130977561-130978141 c
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q36"
     gene            1..5053
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /note="collagen type II alpha 1 chain"
                     /db_xref="GeneID:25412"
                     /db_xref="RGD:2375"
     exon            1..240
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..4619
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; collagen alpha-1(II) chain; alpha-1 type II
                     collagen; Procollagen II alpha 1; procollagen, type II,
                     alpha 1; collagen, type II, alpha 1; collagen type II
                     (Col2A1) gene, enhancer region"
                     /codon_start=1
                     /product="collagen alpha-1(II) chain isoform 1 precursor"
                     /protein_id="NP_001401825.1"
                     /db_xref="GeneID:25412"
                     /db_xref="RGD:2375"
                     /translation="MIRLGAPQSLVLLTLLIATVLQCQGQDAQEAGSCLQNGQRYKDK
                     DVWKPSSCRICVCDTGNVLCDDIICEDPDCLNPEIPFGECCPICPADLATASGKLGPK
                     GQKGEPGDIKDIIGPKGPPGPQGPAGEQGPRGDRGDKGERGAPGPRGRDGEPGTPGNP
                     GPPGPPGPPGPPGLGGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPAGAP
                     GPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKAGERGLPGPQGARG
                     FPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGR
                     TGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSR
                     GEPGNPGSPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLG
                     PKGQTGEPGIAGFKGEQGPKGETGPAGPQGAPGPAGEEGKRGARGEPGGAGPIGPPGE
                     RGAPGNRGFPGQDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPGARGLTGRP
                     GDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAG
                     APGLRGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGD
                     QGIPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPP
                     GAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAG
                     ANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGDQGEAGQ
                     KGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGSNGNP
                     GPAGPPGPAGKDGPKGARGDTGAPGRAGDPGLQGPAGAPGEKGEPGDDGPSGSDGPPG
                     PQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGP
                     AGEPGREGSPGADGPPGRDGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDR
                     GEAGAQGPMGPSGPAGARGIAGPQGPRGDKGEAGEPGERGLKGHRGFTGLQGLPGPPG
                     PSGDQGTSGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPGPRGRSGETGPAGPPGN
                     PGPPGPPGPPGPGIDMSAFAGLGQREKGPDPLQYMRADEADSTLRQHDVEVDATLKSL
                     NNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETG
                     ETCVYPNPATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFL
                     RLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKD
                     GCTKHTGKWGKTIIEYRSQKTSRLPIVDIAPMDIGGPDQEFGVDIGPVCFL"
     exon            241..444
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            445..461
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            462..494
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            495..527
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            528..581
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            582..686
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            687..764
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            765..809
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            810..863
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            864..917
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            918..971
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1025
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1079
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1124
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1178
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1223
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1277
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1376
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1421
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1520
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1574
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1682
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1736
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1835
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1889
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1988
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2042
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2096
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2150
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2204
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2249
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2348
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2456
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2457..2510
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2564
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2618
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2619..2672
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2673..2780
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2781..2834
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2888
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            2889..3050
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3158
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3159..3266
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3320
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3321..3428
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3429..3482
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..3590
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3591..3644
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3645..3752
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            3753..4041
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            4042..4229
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            4230..4472
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
     exon            4473..5053
                     /gene="Col2a1"
                     /gene_synonym="CG2A1A; COLLII"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcagagcgcc gctgggctgc cgggtctcct gcctcctcct cctgctccta gagcctcctg
       61 catgagggcg cggtagagac ccggacccgc tccgtgctct gccgcctcgc cgagcttcgc
      121 ccgggccaag gctctgccag gcctcgcggt gagccatgat ccgcctcggg gctccccagt
      181 cgctggtgct gctgacgctg ctcatcgcca cggtcctaca atgtcagggc caggatgccc
      241 aggaggctgg cagctgtctg cagaatgggc agaggtataa agataaggat gtatggaagc
      301 cctcgtcctg ccgcatctgt gtgtgtgaca ctgggaatgt cctctgcgat gacataatct
      361 gtgaagaccc agactgcctc aaccccgaga tccccttcgg agagtgctgt cccatctgcc
      421 cagctgacct cgccactgcc agtggaaaat tagggccaaa ggggcagaaa ggagaacctg
      481 gagatatcaa agatatcata ggacctaaag gacctcctgg ccctcaggga cctgccggtg
      541 aacaaggacc cagaggtgac cgtggtgaca agggagagag gggtgcacct ggaccccgtg
      601 gcagagatgg agaacctggt acccctggaa atcctggtcc ccctggccct ccaggccccc
      661 ctggtccccc tggccttggt ggaggaaact ttgcagccca gatggctgga ggatttgacg
      721 agaaggctgg tggtgcccag atgggagtca tgcaaggccc catgggcccc atgggacctc
      781 gtggaccccc aggccccgcc ggtgcccccg gccctcaagg atttcaaggc aatcctggtg
      841 aacctggcga gcctggtgtc tctggtccca tgggtccccg aggtcctcca ggtcctgctg
      901 gaaaacctgg tgatgatggt gaagctggaa agcccggaaa ggccggagaa agaggcctcc
      961 ctggtcctca gggtgctcgt ggattcccgg gaacccccgg tcttcctggt gtcaagggtc
     1021 acagaggtta cccaggcctc gatggtgcta agggagaagc tggtgctcca ggtgtgaagg
     1081 gtgagagcgg ttcccctggt gagaacggat ccccaggccc aatgggtccc cgtggcctgc
     1141 ctggcgagag aggacggact ggccctgctg gtgctgctgg tgctcgaggc aacgacggcc
     1201 agccaggccc tgctggacct ccgggtcctg ttggtcctgc aggtggtcct ggcttccctg
     1261 gtgctcctgg tgccaagggc gaagctggtc ccactggtgc ccgtggtcct gaaggtgctc
     1321 aaggttctcg tggcgaaccc ggcaatcccg gatcccccgg gcccgcaggc gcttctggta
     1381 acccagggac tgatggtatt cccggagcca aaggatctgc tggcgctcct ggaattgctg
     1441 gtgcccctgg cttccctggg ccccgtggcc ctcccggtcc tcaaggtgca actggtcctc
     1501 tgggccccaa aggtcagacg ggtgagcccg gcatcgctgg cttcaaaggt gaacaaggcc
     1561 ccaagggaga gactggacct gctgggcccc agggagcccc tggccctgct ggtgaagaag
     1621 gaaaacgagg tgctcgagga gagcccggtg gcgctggacc tatcggcccc cctggagaga
     1681 gaggtgctcc tggcaaccgt ggtttcccag gtcaagatgg tctggcaggt cccaagggtg
     1741 cccctggaga gcgagggcct agtggcctgg ctggtcctaa gggtgccaat ggtgacccgg
     1801 gtcgtcctgg agaacctggt cttcctggag ccaggggtct tactggccgc cctggtgatg
     1861 ctggtcctca aggcaaagtt ggtccttctg gagcccctgg agaagacggt cgccctggtc
     1921 ctcctggtcc tcagggagct cgtgggcagc ctggcgtcat gggtttccct ggccccaaag
     1981 gagccaatgg cgagcctggc aaagctggtg agaaaggact ggctggtgct cctggtctga
     2041 gaggtcttcc tggcaaagat ggtgagacag gagccgcagg accccccggc cccagcggac
     2101 ctgctggtga acgaggcgag cagggtgctc ctgggccatc agggttccag ggacttcctg
     2161 gccctcctgg tcccccgggt gaaggtggaa agcaaggtga ccagggtatt cctggtgaag
     2221 ctggagcccc tggcctcgtt ggtccccggg gtgaacgagg tttcccaggt gaacgtggct
     2281 ctcccggtgc tcagggcctc cagggtcccc gaggcctccc tggcactcct ggtactgatg
     2341 gtcccaaagg tgcggctggc ccagatggtc cccctggggc tcagggccct ccaggtctac
     2401 agggaatgcc tggtgagagg ggagcagctg gtattgctgg acccaaggga gacagaggtg
     2461 atgttggcga gaaaggccca gagggagctc ctggaaaaga tggtggccga ggtcttactg
     2521 ggcccatcgg gcccccagga ccagcaggtg ccaatggcga gaagggagaa gtcggacctc
     2581 ctggtccttc aggaagtact ggagctcgag gcgccccggg tgagcgcgga gagactgggc
     2641 cacctggacc tgctggattc gctggccctc ctggtgctga tggccagcct ggtgccaagg
     2701 gcgatcaagg agaagctgga cagaaaggtg acgctggtgc ccctggccca caaggcccct
     2761 caggagctcc cgggccacag ggtcctactg gagtgactgg tcctaaggga gcccggggcg
     2821 cccaaggccc accgggagcc accggattcc ctggagctgc tggccgagtt ggacccccag
     2881 gttctaatgg caaccctggg cccgccggtc cccctggtcc tgctggaaaa gatggtccca
     2941 aaggtgctcg aggagacact ggtgcccctg gcagagctgg tgaccctgga cttcaaggac
     3001 ccgcaggagc tcctggagag aaaggcgaac ctggagatga cggtccctct ggttctgatg
     3061 gtcccccagg tccccagggg ctggctggtc aaaggggcat tgttggtctg cctggtcagc
     3121 gtggtgagag aggattccca ggccttcccg gcccatcggg tgagcccggc aagcagggtg
     3181 cacctggtgc atctggagac agaggtcctc ctggtcctgt ggggcctcct ggcttgacag
     3241 gacctgcagg tgaacctgga cgagagggca gccctggtgc tgatggaccc cctggcagag
     3301 atggtgcggc tggagtcaag ggtgatcgtg gtgagactgg tgcacttggt gctcctggag
     3361 ctcctgggcc cccaggctct cctggtcctg ctggcccaac tggcaaacaa ggagacagag
     3421 gagaggctgg tgcacaaggt cctatgggcc cctcaggacc tgctggagcc cgtggaattg
     3481 ctggccctca aggcccccga ggtgacaaag gagaagctgg agagcctggc gagagaggac
     3541 tgaaggggca ccgaggtttc actggactgc agggtctgcc tggccccccg ggtccttctg
     3601 gagatcaggg tacttctggc cctgctggtc cttccggccc tagaggtcca cctggccctg
     3661 ttggtccctc tggcaaagat ggctctaatg gaatccctgg ccccatcggg cctccaggtc
     3721 cccgtggacg ctcaggagaa actggccctg ctggtcctcc tggaaatcct ggtccccctg
     3781 gccctccggg tcctcctggt cctggcatcg acatgtcagc ctttgctggc ttaggacaga
     3841 gagagaaggg ccccgatccc ctgcagtaca tgcgggccga cgaggcagac agtaccttga
     3901 gacagcatga cgtcgaggtg gacgccacgc tcaagtcgct gaacaaccag atcgagagca
     3961 tccgcagccc tgatggctcc cgcaagaatc ccgctcgcac ctgccaggac ctgaaactct
     4021 gccacccaga gtggaagagc ggagactact ggattgatcc caaccagggc tgcaccttgg
     4081 acgccatgaa agtcttctgc aacatggaga ctggcgagac ttgcgtctac cccaacccag
     4141 cgactgtgcc tcggaagaac tggtggagca gcaagagcaa ggagaagaag cacatctggt
     4201 ttggagagac catgaacggc ggcttccact tcagctacgg cgacggcaac ctggctccca
     4261 acaccgctaa cgtccagatg actttcctcc gtctactgtc cactgagggc tcccagaaca
     4321 tcacctacca ctgtaagaac agcattgcct acctggacga agcagccggc aacctcaaga
     4381 aggccttgct catccagggc tccaatgatg tggagatgag ggccgagggc aacagcaggt
     4441 tcacgtacac tgccctgaag gatggctgca cgaaacacac cggtaagtgg ggcaagacca
     4501 tcatcgagta ccgatcacag aagacctcac gccttcccat tgttgacatt gcacccatgg
     4561 acatcggagg gcctgatcag gaatttggtg tggacatagg gcctgtctgt ttcttgtaaa
     4621 accctcaacc ccaaaacaac acaatccatt gcgaacccaa aggacccaaa tactttctaa
     4681 ccgcagtcac tctaggatct gcactgaatg gctgacctga cctgatattc aactatccta
     4741 acctcacagt ccggaccatt ctcccctttc taagagacct gaactgggca gactgcaaaa
     4801 taaaatctcg aagttctatt tatttattgt cttcctgtaa gacctttggg tcaaggcgga
     4861 gacaggaact atctggtgtg agtcagacgc cccccgagtg actgctccca gcccagccag
     4921 aagaccccta caggtgctgg gcgcaggaac tgtgtgtcct acacaatggt gctattctgt
     4981 gtcaaacacc tctgtatttt ttaaaacacc aattgatatt aaaaaccaaa aaaaaaaaaa
     5041 tcattggaaa gga
//
