LOCUS       NM_001113366            3272 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Rattus norvegicus connector enhancer of kinase suppressor of Ras 2
            (Cnksr2), transcript variant 1, mRNA.
ACCESSION   NM_001113366
VERSION     NM_001113366.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Zieger,H.L., Kunde,S.A., Rademacher,N., Schmerl,B. and
            Shoichet,S.A.
  TITLE     Disease-associated synaptic scaffold protein CNK2 modulates PSD
            size and influences localisation of the regulatory kinase TNIK
  JOURNAL   Sci Rep 10 (1), 5709 (2020)
   PUBMED   32235845
  REMARK    GeneRIF: Disease-associated synaptic scaffold protein CNK2
            modulates PSD size and influences localisation of the regulatory
            kinase TNIK.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Lim,J., Ritt,D.A., Zhou,M. and Morrison,D.K.
  TITLE     The CNK2 scaffold interacts with vilse and modulates Rac cycling
            during spine morphogenesis in hippocampal neurons
  JOURNAL   Curr Biol 24 (7), 786-792 (2014)
   PUBMED   24656827
REFERENCE   3  (bases 1 to 3272)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   4  (bases 1 to 3272)
  AUTHORS   Munton,R.P., Tweedie-Cullen,R., Livingstone-Zatchej,M.,
            Weinandy,F., Waidelich,M., Longo,D., Gehrig,P., Potthast,F.,
            Rutishauser,D., Gerrits,B., Panse,C., Schlapbach,R. and Mansuy,I.M.
  TITLE     Qualitative and quantitative analyses of protein phosphorylation in
            naive and stimulated mouse synaptosomal preparations
  JOURNAL   Mol Cell Proteomics 6 (2), 283-293 (2007)
   PUBMED   17114649
REFERENCE   5  (bases 1 to 3272)
  AUTHORS   Lanigan,T.M., Liu,A., Huang,Y.Z., Mei,L., Margolis,B. and Guan,K.L.
  TITLE     Human homologue of Drosophila CNK interacts with Ras effector
            proteins Raf and Rlf
  JOURNAL   FASEB J 17 (14), 2048-2060 (2003)
   PUBMED   14597674
REFERENCE   6  (bases 1 to 3272)
  AUTHORS   Ohtakara,K., Nishizawa,M., Izawa,I., Hata,Y., Matsushima,S.,
            Taki,W., Inada,H., Takai,Y. and Inagaki,M.
  TITLE     Densin-180, a synaptic protein, links to PSD-95 through its direct
            interaction with MAGUIN-1
  JOURNAL   Genes Cells 7 (11), 1149-1160 (2002)
   PUBMED   12390249
REFERENCE   7  (bases 1 to 3272)
  AUTHORS   Iida,J., Nishimura,W., Yao,I. and Hata,Y.
  TITLE     Synaptic localization of membrane-associated guanylate
            kinase-interacting protein mediated by the pleckstrin homology
            domain
  JOURNAL   Eur J Neurosci 15 (9), 1493-1498 (2002)
   PUBMED   12028359
  REMARK    GeneRIF: MAGUIN-1 and -2 are recruited to synapses by the
            pleckstrin homology domain and that MAGUIN-1 subsequently interacts
            with PSD-95 at synapses.
REFERENCE   8  (bases 1 to 3272)
  AUTHORS   Yao,I., Hata,Y., Ide,N., Hirao,K., Deguchi,M., Nishioka,H.,
            Mizoguchi,A. and Takai,Y.
  TITLE     MAGUIN, a novel neuronal membrane-associated guanylate
            kinase-interacting protein
  JOURNAL   J Biol Chem 274 (17), 11889-11896 (1999)
   PUBMED   10207009
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF102854.1, JAXUCZ010000021.1 and AF102853.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF102853.1, SRR26643298.31879.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-883               AF102854.1         290-1172
            884-914             JAXUCZ010000021.1  40842200-40842230
            915-3272            AF102853.1         742-3099
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq21"
     gene            1..3272
                     /gene="Cnksr2"
                     /note="connector enhancer of kinase suppressor of Ras 2"
                     /db_xref="GeneID:59322"
                     /db_xref="RGD:708454"
     exon            1..237
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..3272
                     /gene="Cnksr2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     maguin-1; maguin-2; CNK2; CNK homolog protein 2; connector
                     enhancer of KSR 2; membrane-associated guanylate
                     kinase-interacting protein"
                     /codon_start=1
                     /product="connector enhancer of kinase suppressor of ras 2
                     isoform 1"
                     /protein_id="NP_001106837.1"
                     /db_xref="GeneID:59322"
                     /db_xref="RGD:708454"
                     /translation="MALIMEPVSKWSPSQVVDWMKGLDDCLQQYIKNFEREKISGDQL
                     LRITHQELEDLGVSRIGHQELILEAVDLLCALNYGLETENLKTLSHKLNASAKNLQNF
                     ITGRRRSGHYDGRTSRKLPNDFLTSVVDLIGAAKSLLAWLDRSPFAAVTDYSVTRNNV
                     IQLCLELTTIVQQDCTVYETENKILHVCKTLSGVCDHIISLSSDPLVSQSAHLEVIQL
                     ANIKPSEGLGMYIKSTYDGLHVITGTTENSPADRCKKIHAGDEVIQVNHQTVVGWQLK
                     NLVNALREDPSGVILTLKKRPQSMLTSAPALLKNMRWKPLALQPLIPRSPTSSVATPS
                     STISTPTKRDSSALQDLYIPPPPAEPYIPRDEKGNLPCEDLRGHMVGKPVHKGSESPN
                     SFLDQEYRKRFNIVEEDTVLYCYEYEKGRSSSQGRRESTPTYGKLRPISMPVEYNWVG
                     DYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKKGDKSTSPTH
                     YSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGAIAGKSKRRISCK
                     DLGRGDCEGWLWKKKDAKSYFSQKWKKYWFVLKDASLYWYINEEDEKAEGFISLPEFK
                     IDRASECRKKYAFKACHPKIKSFYFAAEHLDDMNRWLNRINMLTAGYAERERIKQEQD
                     YWSESDKEEADTPSTPKQDSPPPPYDTYPRPPSMSCASPYVEAKHSRLSSTETSQSQS
                     SHEEFRQEVTGSSAVSPIRKTASQRRSWQDLIETPLTSSGLHYLQTLPLEDSVFSDSA
                     AISPEHRRQSTLPTQKCHLQDHYGPYPLAESERMQVLNGNGGKPRSFTLPRDSGFNHC
                     CLNAPVSACDPQDDIQPPEVEEEEEEEEEEAAGENIGEKNENREEKLGDSLQDLYRAL
                     EEASLSPLGEHRISTKIEYKLSFIKRCNDPVMNEKLHRLRILKSTLKAREGEVAIIDK
                     VLDNPDLTSKEFQQWKQMYLDLFLDICQNTTSNDPLSISSEVDVITSSLTHTHSYIET
                     HV"
     exon            238..401
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            402..604
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            605..692
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            693..734
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            735..854
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            855..914
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            915..983
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1130
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1264
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1476
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1566
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1781
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1830
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..2003
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2077
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2149
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2217
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2318
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2859
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2860..3056
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
     exon            3057..3272
                     /gene="Cnksr2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggccgcct ccgacgctcc agcctccgga cggacacaca aaagccaccc tctccctgtc
       61 gcccacccca ggaggctgcg gccggccact gaccccacct gagggcgcag cgggctgcgg
      121 acttggcttt ctgtccgagc tccgcgtccc acgcggcacc aaccccgccg cccatggctc
      181 tgataatgga accagtgagc aaatggtctc cgagtcaagt agtggactgg atgaaaggtc
      241 tcgatgactg cttacagcag tatattaaga actttgagag ggagaagatc agtggagacc
      301 agctgctacg cattacacat caggagctag aagatctggg agtcagccgt attggccatc
      361 aggagctgat cttggaggca gtcgaccttc tgtgtgcact gaattatggc ttagaaacag
      421 aaaatctaaa aaccctttct cacaagttga atgcgtctgc caaaaatcta cagaacttca
      481 taacaggaag gaggaggagc ggccattatg atgggaggac cagccgaaaa ttaccaaatg
      541 actttttgac ctcagttgtg gatctgattg gagcagccaa gagtcttctt gcttggttgg
      601 acaggtcacc atttgctgct gtgacagact attcagtcac aagaaataat gtcatacaac
      661 tctgcttgga gttaacaaca attgtgcaac aggattgcac tgtctatgaa actgaaaata
      721 aaattcttca tgtgtgtaaa actctttctg gggtctgtga ccacatcata tccctgtcgt
      781 cagatcctct ggtttcacag tctgctcacc ttgaagtgat tcagctggca aatatcaaac
      841 caagtgaagg gctgggtatg tatattaaat ccacatatga tggcctccat gtaattacgg
      901 gaaccacaga aaattcacct gcagatcggt gcaagaaaat ccacgctggg gatgaagtga
      961 ttcaagtcaa tcatcagact gtggtggggt ggcagttgaa aaatttggtg aatgcactac
     1021 gagaggaccc gagtggtgtt atcttaactt tgaaaaagcg acctcagagc atgcttacct
     1081 cagcaccagc tttactgaaa aatatgagat ggaagcccct tgctctgcag cctcttatac
     1141 ctagaagtcc cacaagcagc gttgccacgc cttccagcac catcagtaca cccaccaaaa
     1201 gagacagttc tgctctccag gatctctaca tcccccctcc tcctgcagaa ccatacattc
     1261 ccagggacga aaaaggaaac cttccttgtg aagatctcag aggacatatg gtgggcaagc
     1321 cagttcataa gggatctgaa tcccccaatt catttctgga ccaggaatat cgaaagaggt
     1381 ttaacattgt tgaagaagac actgtcttat attgctatga atatgaaaaa ggacgatcaa
     1441 gtagtcaagg aagacgagaa agcacaccaa cctatggcaa gctacgacct atatctatgc
     1501 cagtcgaata taattgggtg ggggactatg aagatccaaa taagatgaag agagatagta
     1561 gaagagaaaa ctctctactt cgatacatga gcaatgaaaa aattgctcaa gaagaataca
     1621 tgtttcagag aaacagcaaa aaggacacag ggaagaagtc aaaaaagaag ggtgataaga
     1681 gtactagccc aactcactac tccttgctgc ctagtttgca aatggatgca ttgagacaag
     1741 acatcatggg cacaccagtg ccagaaacca cactgtacca tacatttcag cagtcctctc
     1801 tgcagcacaa atcaaagaag aaaaacaaag gagctatagc aggcaagagc aaaagaagaa
     1861 tttcttgcaa ggatcttggc cgtggtgact gtgaaggatg gctttggaaa aagaaagatg
     1921 caaagagcta tttttcacag aaatggaaga agtactggtt tgtcctaaag gatgcatcct
     1981 tgtactggta tattaatgaa gaggatgaaa aagcagaggg attcatcagt ctgcctgaat
     2041 ttaaaatcga tagagccagt gaatgccgca agaaatatgc attcaaggcc tgccatccta
     2101 aaatcaaaag cttttatttc gctgctgagc atctcgatga catgaacagg tggcttaata
     2161 gaattaacat gctgactgca ggatatgccg aaagagagag gattaaacaa gaacaagatt
     2221 actggagtga gagtgacaaa gaagaagcag atactccatc aacaccaaaa caagacagcc
     2281 ctccaccccc atatgacaca tacccacggc ctccctctat gagttgtgcc agtccttatg
     2341 tggaagcaaa acacagccgg ctttcctcca cagagacctc tcagtctcag tcttcacatg
     2401 aggaatttcg acaggaagtt actgggagca gtgctgtgtc ccccattcgc aagacagcca
     2461 gtcagcgccg ctcctggcag gatttaatcg agacgccact gacaagttca ggcttacact
     2521 atcttcagac tctgcctctg gaagattctg tcttctctga ctcggcagcc atctcccctg
     2581 agcacaggcg gcagtccact cttccaactc agaagtgcca cctccaggat cattatggcc
     2641 catacccttt agctgagagc gagaggatgc aagtgttgaa tggcaatggg ggcaagcctc
     2701 gaagttttac tctgcctcga gatagcgggt tcaaccattg ctgtctgaac gcaccagtta
     2761 gtgcctgcga tccacaagat gacatacagc caccagaggt ggaggaagaa gaggaggagg
     2821 aggaagagga ggcagcagga gaaaacatag gcgagaaaaa tgaaaatagg gaagaaaagt
     2881 taggagactc cttgcaagat ttatacaggg cactggagga agccagtctg tcaccactag
     2941 gagaacatcg catttcaacc aagatcgaat acaaactatc gttcataaaa cgatgcaatg
     3001 atcctgtgat gaatgagaag ctgcacaggc tgagaattct taaaagcact ttaaaggcca
     3061 gagaagggga agtagccatt attgataaag tcctagacaa tccagacttg acatctaaag
     3121 aattccaaca atggaagcag atgtacctcg accttttctt ggatatctgt caaaacacca
     3181 cctcaaatga cccactaagt atttcttctg aagtagatgt tatcacttcc tctctaacac
     3241 atactcattc gtacattgag acacacgtgt aa
//
