LOCUS       NP_445948                575 aa            linear   ROD 09-FEB-2025
DEFINITION  cyclic nucleotide-gated channel alpha-4 [Rattus norvegicus].
ACCESSION   NP_445948 XP_346556
VERSION     NP_445948.2
DBSOURCE    REFSEQ: accession NM_053496.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 575)
  AUTHORS   Nache,V., Wongsamitkul,N., Kusch,J., Zimmer,T., Schwede,F. and
            Benndorf,K.
  TITLE     Deciphering the function of the CNGB1b subunit in olfactory CNG
            channels
  JOURNAL   Sci Rep 6, 29378 (2016)
   PUBMED   27405959
  REMARK    Erratum:[Sci Rep. 2018 Jun 28;8:47000. doi: 10.1038/srep47000..
            PMID: 29952375]
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 575)
  AUTHORS   Nache,V., Zimmer,T., Wongsamitkul,N., Schmauder,R., Kusch,J.,
            Reinhardt,L., Bonigk,W., Seifert,R., Biskup,C., Schwede,F. and
            Benndorf,K.
  TITLE     Differential regulation by cyclic nucleotides of the CNGA4 and
            CNGB1b subunits in olfactory cyclic nucleotide-gated channels
  JOURNAL   Sci Signal 5 (232), ra48 (2012)
   PUBMED   22786723
  REMARK    GeneRIF: cGMP activated olfactory CNG channels by binding to the
            two CNGA2 subunits and the CNGA4 subunit.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 575)
  AUTHORS   Berridge,M.J.
  TITLE     Calcium signalling remodelling and disease
  JOURNAL   Biochem Soc Trans 40 (2), 297-309 (2012)
   PUBMED   22435804
  REMARK    Review article
REFERENCE   4  (residues 1 to 575)
  AUTHORS   Zheng,J. and Zagotta,W.N.
  TITLE     Stoichiometry and assembly of olfactory cyclic nucleotide-gated
            channels
  JOURNAL   Neuron 42 (3), 411-421 (2004)
   PUBMED   15134638
REFERENCE   5  (residues 1 to 575)
  AUTHORS   Kelliher,K.R., Ziesmann,J., Munger,S.D., Reed,R.R. and Zufall,F.
  TITLE     Importance of the CNGA4 channel gene for odor discrimination and
            adaptation in behaving mice
  JOURNAL   Proc Natl Acad Sci U S A 100 (7), 4299-4304 (2003)
   PUBMED   12649326
REFERENCE   6  (residues 1 to 575)
  AUTHORS   Bonigk,W., Bradley,J., Muller,F., Sesti,F., Boekhoff,I.,
            Ronnett,G.V., Kaupp,U.B. and Frings,S.
  TITLE     The native rat olfactory cyclic nucleotide-gated channel is
            composed of three distinct subunits
  JOURNAL   J Neurosci 19 (13), 5332-5347 (1999)
   PUBMED   10377344
REFERENCE   7  (residues 1 to 575)
  AUTHORS   Dzeja,C., Hagen,V., Kaupp,U.B. and Frings,S.
  TITLE     Ca2+ permeation in cyclic nucleotide-gated channels
  JOURNAL   EMBO J 18 (1), 131-144 (1999)
   PUBMED   9878057
REFERENCE   8  (residues 1 to 575)
  AUTHORS   Bradley,J., Zhang,Y., Bakin,R., Lester,H.A., Ronnett,G.V. and
            Zinn,K.
  TITLE     Functional expression of the heteromeric 'olfactory' cyclic
            nucleotide-gated channel in the hippocampus: a potential effector
            of synaptic plasticity in brain neurons
  JOURNAL   J Neurosci 17 (6), 1993-2005 (1997)
   PUBMED   9045728
REFERENCE   9  (residues 1 to 575)
  AUTHORS   Bradley,J., Li,J., Davidson,N., Lester,H.A. and Zinn,K.
  TITLE     Heteromeric olfactory cyclic nucleotide-gated channels: a subunit
            that confers increased sensitivity to cAMP
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 8890-8894 (1994)
   PUBMED   7522325
REFERENCE   10 (residues 1 to 575)
  AUTHORS   Liman,E.R. and Buck,L.B.
  TITLE     A second subunit of the olfactory cyclic nucleotide-gated channel
            confers high sensitivity to cAMP
  JOURNAL   Neuron 13 (3), 611-621 (1994)
   PUBMED   7522482
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            U12623.1 and JAXUCZ010000001.1.
            
            On Oct 12, 2007 this sequence version replaced NP_445948.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U12425.1, SRR26360181.360156.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Rattus norvegicus"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     Protein         1..575
                     /product="cyclic nucleotide-gated channel alpha-4"
                     /note="cyclic nucleotide gated cation channel; olfactory
                     cyclic nucleotide-gated channel subunit 2; cyclic
                     nucleotide gated channel alpha 4; cyclic nucleotide-gated
                     cation channel alpha-4; CNCalpha4 C; CNG channel alpha-4;
                     cyclic nucleotide-gated olfactory channel subunit OCNC2"
                     /calculated_mol_wt=65660
     Region          34..273
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Site            39..60
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Site            71..91
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Site            117..135
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Site            141..159
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Region          164..272
                     /region_name="Ion conduction pathway.
                     /evidence=ECO:0000250|UniProtKB:P29973"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Site            167..190
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Site            214..248
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Region          231..234
                     /region_name="Selectivity filter.
                     /evidence=ECO:0000250|UniProtKB:P29973"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Site            249..273
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Site            258
                     /site_type="other"
                     /note="Central gate.
                     /evidence=ECO:0000250|UniProtKB:P29973; propagated from
                     UniProtKB/Swiss-Prot (Q64359.1)"
     Region          274..350
                     /region_name="C-linker.
                     /evidence=ECO:0000250|UniProtKB:P29973"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Region          292..302
                     /region_name="IQ-type. /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Region          348..464
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Region          354..474
                     /region_name="Cyclic nucleotide-binding domain.
                     /evidence=ECO:0000250|UniProtKB:P29973"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     Site            order(414..415,430..432)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(452..454,458..460)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     Region          464..534
                     /region_name="CLZ"
                     /note="C-terminal leucine zipper domain of cyclic
                     nucleotide-gated channels; pfam16526"
                     /db_xref="CDD:465160"
     Region          537..575
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64359.1)"
     CDS             1..575
                     /gene="Cnga4"
                     /gene_synonym="Cgn2; CNG-4; CNG4; CNG5; OCNC2; rOCNC2"
                     /coded_by="NM_053496.2:108..1835"
                     /db_xref="GeneID:85258"
                     /db_xref="RGD:619844"
ORIGIN      
        1 msqdgkvktt estppaptka rkwlpvldps gdyyywwlnt mvfpimynli ivvcracfpd
       61 lqhsylvawf vldytsdlly lldigvrfht gfleqgilvv dkgmiasryv rtwsflldla
      121 slvptdaayv qlgphiptlr lnrflrvprl feafdrtetr taypnafria klmlyifvvi
      181 hwnsclyfal srylgfgrda wvypdpaqpg ferlrrqyly sfyfstlilt tvgdtplpdr
      241 eeeylfmvgd fllavmgfat imgsmssviy nmntadaafy pdhalvkkym klqhvnkrle
      301 rrvidwyqhl qinkkmtnev ailqhlperl raevavsvhl stlsrvqifq nceaslleel
      361 vlklqpqtys pgeyvcrkgd igremyiire gqlavvaddg vtqyavlgag lyfgeisiin
      421 ikgnmsgnrr tanikslgys dlfclskedl revlseypqa qavmeekgre illkmnkldv
      481 naeaaeialq eatesrlkgl dqqlddlqtk farllaeles salkiayrie rlewqtrewp
      541 mpedmgeadd eaepgegtsk dgegkagqeg psdie
//
