LOCUS       NM_053021               1860 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Rattus norvegicus clusterin (Clu), transcript variant 5, mRNA.
ACCESSION   NM_053021 NM_012679 XM_001053032 XM_001053033
VERSION     NM_053021.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Yang,N., Dong,B., Song,Y., Li,Y., Kou,L. and Qin,Q.
  TITLE     Apolipoprotein J Attenuates Vascular Restenosis by Promoting
            Autophagy and Inhibiting the Proliferation and Migration of
            Vascular Smooth Muscle Cells
  JOURNAL   J Cardiovasc Transl Res 15 (5), 1086-1099 (2022)
   PUBMED   35244876
  REMARK    GeneRIF: Apolipoprotein J Attenuates Vascular Restenosis by
            Promoting Autophagy and Inhibiting the Proliferation and Migration
            of Vascular Smooth Muscle Cells.
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Rodriguez-Rivera,C., Gonzalez-Martin,C., Fernandez-Millan,E.,
            Alvarez,C., Escriva,F. and Alguacil,L.F.
  TITLE     Perinatal undernourishment provokes long-lasting alterations of
            clusterin and fumarate hydratase expression in the rat nucleus
            accumbens
  JOURNAL   Nutr Neurosci 25 (9), 1796-1800 (2022)
   PUBMED   33755527
  REMARK    GeneRIF: Perinatal undernourishment provokes long-lasting
            alterations of clusterin and fumarate hydratase expression in the
            rat nucleus accumbens.
REFERENCE   3  (bases 1 to 1860)
  AUTHORS   Vargas,A., Yamamoto,K.L., Craft,C.M. and Lee,E.J.
  TITLE     Clusterin enhances cell survival by suppressing neuronal
            nitric-oxide synthase expression in the rhodopsin S334ter-line3
            retinitis pigmentosa model
  JOURNAL   Brain Res 1768, 147575 (2021)
   PUBMED   34242654
  REMARK    GeneRIF: Clusterin enhances cell survival by suppressing neuronal
            nitric-oxide synthase expression in the rhodopsin S334ter-line3
            retinitis pigmentosa model.
REFERENCE   4  (bases 1 to 1860)
  AUTHORS   Tian,Y., Xiao,Y.H., Geng,T., Sun,C., Gu,J., Tang,K.F., Liu,B.,
            Liu,Y.M. and Sun,F.
  TITLE     Clusterin suppresses spermatogenic cell apoptosis to alleviate
            diabetes-induced testicular damage by inhibiting autophagy via the
            PI3K/AKT/mTOR axis
  JOURNAL   Biol Cell 113 (1), 14-27 (2021)
   PUBMED   32942336
  REMARK    GeneRIF: Clusterin suppresses spermatogenic cell apoptosis to
            alleviate diabetes-induced testicular damage by inhibiting
            autophagy via the PI3K/AKT/mTOR axis.
REFERENCE   5  (bases 1 to 1860)
  AUTHORS   Das Gupta,S., Lipponen,A., Paldanius,K.M.A., Puhakka,N. and
            Pitkanen,A.
  TITLE     Dynamics of clusterin protein expression in the brain and plasma
            following experimental traumatic brain injury
  JOURNAL   Sci Rep 9 (1), 20208 (2019)
   PUBMED   31882899
  REMARK    GeneRIF: Dynamics of clusterin protein expression in the brain and
            plasma following experimental traumatic brain injury.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1860)
  AUTHORS   Smith,S.B., Bora,N., McCool,D., Kutty,G., Wong,P., Kutty,R.K. and
            Wiggert,B.
  TITLE     Photoreceptor cells in the vitiligo mouse die by apoptosis.
            TRPM-2/clusterin expression is increased in the neural retina and
            in the retinal pigment epithelium
  JOURNAL   Invest Ophthalmol Vis Sci 36 (11), 2193-2201 (1995)
   PUBMED   7558712
REFERENCE   7  (bases 1 to 1860)
  AUTHORS   Bandyk,M.G., Sawczuk,I.S., Olsson,C.A., Katz,A.E. and Buttyan,R.
  TITLE     Characterization of the products of a gene expressed during
            androgen-programmed cell death and their potential use as a marker
            of urogenital injury
  JOURNAL   J Urol 143 (2), 407-413 (1990)
   PUBMED   2299741
REFERENCE   8  (bases 1 to 1860)
  AUTHORS   Bettuzzi,S., Hiipakka,R.A., Gilna,P. and Liao,S.T.
  TITLE     Identification of an androgen-repressed mRNA in rat ventral
            prostate as coding for sulphated glycoprotein 2 by cDNA cloning and
            sequence analysis
  JOURNAL   Biochem J 257 (1), 293-296 (1989)
   PUBMED   2920020
REFERENCE   9  (bases 1 to 1860)
  AUTHORS   Cheng,C.Y., Chen,C.L., Feng,Z.M., Marshall,A. and Bardin,C.W.
  TITLE     Rat clusterin isolated from primary Sertoli cell-enriched culture
            medium is sulfated glycoprotein-2 (SGP-2)
  JOURNAL   Biochem Biophys Res Commun 155 (1), 398-404 (1988)
   PUBMED   3415696
REFERENCE   10 (bases 1 to 1860)
  AUTHORS   Collard,M.W. and Griswold,M.D.
  TITLE     Biosynthesis and molecular cloning of sulfated glycoprotein 2
            secreted by rat Sertoli cells
  JOURNAL   Biochemistry 26 (12), 3297-3303 (1987)
   PUBMED   3651384
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Nov 27, 2020 this sequence version replaced NM_053021.2.
            
            Summary: The protein encoded by this gene is a secreted chaperone
            that can under some stress conditions also be found in the cell
            cytosol. It has been suggested to be involved in several basic
            biological events such as cell death, tumor progression, and
            neurodegenerative disorders.[provided by RefSeq, May 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M16975.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                JAXUCZ010000015.1  44336619-44336671
            54-270              JAXUCZ010000015.1  44350167-44350383
            271-391             JAXUCZ010000015.1  44366156-44366276
            392-540             JAXUCZ010000015.1  44367860-44368008
            541-711             JAXUCZ010000015.1  44369380-44369550
            712-1123            JAXUCZ010000015.1  44370106-44370517
            1124-1228           JAXUCZ010000015.1  44371413-44371517
            1229-1455           JAXUCZ010000015.1  44374192-44374418
            1456-1631           JAXUCZ010000015.1  44375162-44375337
            1632-1860           JAXUCZ010000015.1  44375627-44375855
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..1860
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /note="clusterin"
                     /db_xref="GeneID:24854"
                     /db_xref="RGD:3907"
     exon            1..53
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     exon            54..270
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     exon            271..391
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     CDS             298..1641
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 5; sulfated glycoprotein 2; dimeric acid
                     glycoprotein; testosterone repressed prostate message 2;
                     testosterone-repressed prostate message;
                     testostrone-repressed prostate message 2"
                     /codon_start=1
                     /product="clusterin isoform 1 precursor"
                     /protein_id="NP_444180.3"
                     /db_xref="GeneID:24854"
                     /db_xref="RGD:3907"
                     /translation="MKILLLCVALLLTWDNGMVLGEQEFSDNELQELSTQGSRYVNKE
                     IQNAVQGVKHIKTLIEKTNAERKSLLNSLEEAKKKKEGALDDTRDSEMKLKAFPEVCN
                     ETMMALWEECKPCLKHTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRIDS
                     LLESDRQQSQVLDAMQDSFTRASGIIDTLFQDRFFTHEPQDIHHFSPMGFPHKRPHFL
                     YPKSRLVRSLMPLSHYGPLSFHNMFQPFFDMIHQAQQAMDVQLHSPALQFPDVDFLKE
                     GEDDRTVCKEIRHNSTGCLKMKGQCEKCQEILSVDCSTNNPAQANLRQELNDSLQVAE
                     RLTQQYNELLHSLQSKMLNTSSLLEQLNDQFSWVSQLANLTQGDDQYLRVSTVTTHSS
                     DSEVPSRVTEVVVKLFDSDPITVVLPEEVSKDNPKFMDTVAEKALQEYRRKSRME"
     exon            392..540
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     exon            541..711
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     exon            712..1123
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1228
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1455
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1631
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1860
                     /gene="Clu"
                     /gene_synonym="APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2;
                     SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcaagctca cactggctgg acttcctcgc catgacagtc tgtacctcta actgatccca
       61 gggatgatac cacctacatt tggggtggtt cttctcgcct cagttaaacc tctctgggag
      121 caccatcaca gacacccaca gaagtttgtt ccctagatga ttctaggtcc tgtggagttg
      181 acaagattga ccatcacgct ctcagcaatc gggtgaagta aacaccaccg ttgtctccat
      241 ggaaatgctt aactacggct tgctagtaag gactccagac tccaaagagg ccacaccatg
      301 aagattctcc tgctgtgtgt ggcactgctg ctgacctggg acaatggcat ggtcctggga
      361 gagcaggagt tctctgacaa tgagctccaa gaactgtcca ctcaaggaag taggtatgtt
      421 aataaggaga ttcagaacgc cgtccagggg gtgaagcaca taaagaccct catagaaaaa
      481 accaacgcag agcgcaagtc cctgctcaac agtttagagg aagccaaaaa gaagaaagag
      541 ggtgctctag atgacaccag ggattctgaa atgaagctga aggctttccc ggaagtgtgt
      601 aacgagacca tgatggccct ctgggaagag tgtaagccct gcctgaagca cacctgcatg
      661 aagttctacg cacgcgtctg caggagcggc tcggggctgg ttggtcgcca gctagaggag
      721 tttctgaacc agagctcacc cttctacttc tggatgaacg gggaccgcat cgactccctg
      781 ctggagagtg accggcagca gagccaagtc ctagatgcta tgcaggacag cttcactcgg
      841 gcgtctggca tcatagatac gcttttccag gaccggttct tcacccatga gccccaggac
      901 atccaccatt tctcccccat gggcttccca cacaagcggc ctcatttctt gtaccccaag
      961 tcccgcttgg tccgcagcct catgcctctc tcccactacg ggcctctgag cttccacaac
     1021 atgttccagc ctttctttga tatgatacac caggctcaac aggccatgga cgtccagctc
     1081 catagcccag ctttacagtt cccggatgtg gatttcttaa aagaaggtga agatgaccgc
     1141 acagtgtgca aggagatccg ccataactcc acaggatgcc tgaagatgaa gggccagtgt
     1201 gagaagtgcc aagagatctt gtctgtggac tgttcgacca acaatcctgc ccaggctaac
     1261 ctgcgccagg agctaaacga ctcgctccag gtggctgaga ggctgaccca gcagtacaac
     1321 gagctgcttc attccctcca gtccaagatg ctcaacacct catccctgct ggaacagctg
     1381 aacgaccagt tcagctgggt gtcccagctg gctaacctca cacagggcga tgaccagtac
     1441 cttcgggtct ccacagtgac aacccattct tctgactcag aagtcccctc tcgtgtcact
     1501 gaggtggtgg tgaagctgtt tgactctgac cccatcacag tggtgttacc agaagaagtc
     1561 tccaaggata accctaagtt tatggacaca gtggcagaga aagcgctaca ggaataccgc
     1621 aggaaaagcc gcatggaatg agacagaagc atcagttttc tatatgtagg agtctcaagg
     1681 agggaatctc ccagctttcc gaggttgctg cagaccccta gagaactcca catgtctcca
     1741 gcgcctaggc ctccacccca gcagcctctc cttcctctgg gttctgtact ctaatgcctg
     1801 cacttgatgc tctcgggaag aactgcttcc cccacgcaac taatccaata aagccacctt
//
