LOCUS       NM_001007803            2456 bp    mRNA    linear   ROD 16-FEB-2025
DEFINITION  Rattus norvegicus caseinolytic mitochondrial matrix peptidase
            chaperone subunit X (Clpx), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001007803 XM_217179
VERSION     NM_001007803.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Lowth,B.R., Kirstein-Miles,J., Saiyed,T., Brotz-Oesterhelt,H.,
            Morimoto,R.I., Truscott,K.N. and Dougan,D.A.
  TITLE     Substrate recognition and processing by a Walker B mutant of the
            human mitochondrial AAA+ protein CLPX
  JOURNAL   J Struct Biol 179 (2), 193-201 (2012)
   PUBMED   22710082
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Bogenhagen,D.F., Rousseau,D. and Burke,S.
  TITLE     The layered structure of human mitochondrial DNA nucleoids
  JOURNAL   J Biol Chem 283 (6), 3665-3675 (2008)
   PUBMED   18063578
REFERENCE   3  (bases 1 to 2456)
  AUTHORS   Kang,S.G., Dimitrova,M.N., Ortega,J., Ginsburg,A. and Maurizi,M.R.
  TITLE     Human mitochondrial ClpP is a stable heptamer that assembles into a
            tetradecamer in the presence of ClpX
  JOURNAL   J Biol Chem 280 (42), 35424-35432 (2005)
   PUBMED   16115876
REFERENCE   4  (bases 1 to 2456)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   5  (bases 1 to 2456)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   6  (bases 1 to 2456)
  AUTHORS   Kang,S.G., Ortega,J., Singh,S.K., Wang,N., Huang,N.N., Steven,A.C.
            and Maurizi,M.R.
  TITLE     Functional proteolytic complexes of the human mitochondrial
            ATP-dependent protease, hClpXP
  JOURNAL   J Biol Chem 277 (23), 21095-21102 (2002)
   PUBMED   11923310
REFERENCE   7  (bases 1 to 2456)
  AUTHORS   Corydon,T.J., Wilsbech,M., Jespersgaard,C., Andresen,B.S.,
            Borglum,A.D., Pedersen,S., Bolund,L., Gregersen,N. and Bross,P.
  TITLE     Human and mouse mitochondrial orthologs of bacterial ClpX
  JOURNAL   Mamm Genome 11 (10), 899-905 (2000)
   PUBMED   11003706
REFERENCE   8  (bases 1 to 2456)
  AUTHORS   de Sagarra,M.R., Mayo,I., Marco,S., Rodriguez-Vilarino,S.,
            Oliva,J., Carrascosa,J.L. and Casta n,J.G.
  TITLE     Mitochondrial localization and oligomeric structure of HClpP, the
            human homologue of E. coli ClpP
  JOURNAL   J Mol Biol 292 (4), 819-825 (1999)
   PUBMED   10525407
REFERENCE   9  (bases 1 to 2456)
  AUTHORS   Santagata,S., Bhattacharyya,D., Wang,F.H., Singha,N., Hodtsev,A.
            and Spanopoulou,E.
  TITLE     Molecular cloning and characterization of a mouse homolog of
            bacterial ClpX, a novel mammalian class II member of the Hsp100/Clp
            chaperone family
  JOURNAL   J Biol Chem 274 (23), 16311-16319 (1999)
   PUBMED   10347188
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Sep 23, 2022 this sequence version replaced NM_001007803.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085867.1, SRR26643287.20382.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-270               JAXUCZ010000008.1  74700700-74700969
            271-431             JAXUCZ010000008.1  74708913-74709073
            432-549             JAXUCZ010000008.1  74710876-74710993
            550-704             JAXUCZ010000008.1  74718142-74718296
            705-864             JAXUCZ010000008.1  74720410-74720569
            865-906             JAXUCZ010000008.1  74721964-74722005
            907-1083            JAXUCZ010000008.1  74727140-74727316
            1084-1248           JAXUCZ010000008.1  74727796-74727960
            1249-1337           JAXUCZ010000008.1  74729270-74729358
            1338-1502           JAXUCZ010000008.1  74731120-74731284
            1503-1802           JAXUCZ010000008.1  74733117-74733416
            1803-1895           JAXUCZ010000008.1  74734546-74734638
            1896-2002           JAXUCZ010000008.1  74736853-74736959
            2003-2456           JAXUCZ010000008.1  74739791-74740244
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..2456
                     /gene="Clpx"
                     /note="caseinolytic mitochondrial matrix peptidase
                     chaperone subunit X"
                     /db_xref="GeneID:300786"
                     /db_xref="RGD:1304883"
     exon            1..270
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..2093
                     /gene="Clpx"
                     /EC_number="3.6.4.10"
                     /note="ATP-dependent Clp protease ATP-binding subunit
                     clpX-like, mitochondrial; ATP-dependent clpX-like
                     chaperone, mitochondrial; caseinolytic protease X;
                     caseinolytic peptidase X; ClpX caseinolytic protease X
                     homolog; ClpX caseinolytic peptidase X homolog"
                     /codon_start=1
                     /product="ATP-dependent clpX-like chaperone, mitochondrial
                     precursor"
                     /protein_id="NP_001007804.1"
                     /db_xref="GeneID:300786"
                     /db_xref="RGD:1304883"
                     /translation="MSSCGACTCGAAAARLLTTSLTSAQRGISCGRIHVPVLGRLGTL
                     DTQILRRAPLRTFSETPAYFASKDGTNKDGSGDGNKKSVTEGSSKKSGSGNSGKGGNQ
                     LRCPKCGDLCTHVETFVSSTRFVKCEKCHHFFVVLSEADSKKSIIKEPESAAEAVKLA
                     FQQKPPPPPKKIYNYLDKYVVGQSFAKKVLSVAVYNHYKRIYNNIPANLRQQAEVEKQ
                     TSLTPRELEIRRREDEYRFTKLLQIAGISPHGNALGASMQQQGSQQMPQEKRGGEVLD
                     SPHDDIKLEKSNILLLGPTGSGKTLLAQTLAKCLDVPFAICDCTTLTQAGYVGEDIES
                     VIAKLLQDANYNVEKAQQGIVFLDEVDKIGSVPGIHQLRDVGGEGVQQGLLKLLEGTI
                     VNVPEKNSRKLRGETVQVDTTNILFVASGAFNGLDRIISRRKNEKYLGFGTPSNLGKG
                     RRAAAAADLANRSGESNTHQDIEEKDRLLRHVEARDLIEFGMIPEFVGRLPVVVPLHS
                     LDEKTLVQILTEPRNAVIPQYQALFSMDKCELNVTEDALKAIARLALERKTGARGLRS
                     IMEKLLLEPMFEVPNSDIVCVEVDKEVVEGKKEPGYIRAPSKESSEEDYDSGVEEDGW
                     PRQADAANS"
     exon            271..431
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            432..549
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            550..704
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            705..864
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            865..906
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1083
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1248
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1337
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1502
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            1503..1802
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1895
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2002
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2456
                     /gene="Clpx"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 actccggtga gtagaaggcg gtgggacgct gcgatctccc gggcctgact gcctagtggg
       61 aaaaacgctg cgccagcata gaagcggcgg aacggagtgg tgccagtcag cgtaacactc
      121 gagcggcaca atcgggccgc tgagccctct cgatcatcag ggcgcgaagc cccggcctag
      181 gccccgcgga gatgtccagt tgcggcgctt gtacctgcgg cgctgcggct gcccggcttc
      241 tcaccacctc gctcacctcc gcgcagagag gtatttcttg tgggcgcatt catgtgccag
      301 ttttaggaag actggggacc ttggacactc agattctgcg cagagctcct cttagaacct
      361 tttcagaaac accagcatac tttgcctcaa aagatgggac aaacaaggat ggctccggag
      421 atggaaataa gaaatcagtg actgaaggaa gcagcaaaaa atcaggctct gggaattccg
      481 ggaaaggtgg aaaccagctt cgctgtccta aatgtggcga cttgtgcaca cacgtagaga
      541 cctttgtgtc ttccacgcgt tttgttaagt gtgaaaaatg tcatcatttt ttcgttgtgc
      601 tgtcggaagc ggactcaaag aaaagcataa ttaaagaacc agagtcagcc gcagaggccg
      661 tgaagttggc attccagcag aaaccacctc cacccccaaa aaagatctat aactacctcg
      721 acaagtatgt ggttggccag tcgtttgcta agaaagtgct ttcagttgcc gtgtataatc
      781 attataagag aatatataat aatattccag ctaatctgag acagcaagca gaggttgaga
      841 agcagacatc attaacacct agagagttag aaataagaag acgggaggat gagtacagat
      901 ttacaaagtt gcttcagatt gctggaatta gcccacatgg caatgccttg ggagcatcta
      961 tgcagcagca gggaagtcag caaatgcctc aggaaaaacg aggaggcgaa gtactagatt
     1021 ctcctcacga tgatataaaa cttgaaaaaa gtaatattct gctgcttgga ccaactggat
     1081 caggtaaaac cctgctggca caaactttag ctaaatgcct tgatgttcct tttgctatct
     1141 gtgactgtac aactttgact caggctggat atgtaggtga agatattgaa tctgtgattg
     1201 ccaaactact ccaggatgcc aattacaatg tggagaaagc acaacaagga attgtctttc
     1261 tggatgaagt agataagatt ggcagtgtac caggcattca tcagttacgg gatgtaggtg
     1321 gagaaggcgt tcagcaaggt ttactgaaac ttctagaagg cacaatagtc aatgttccag
     1381 aaaagaattc tcgaaagcta cgtggagaga cagttcaagt cgatacaaca aatatcttgt
     1441 ttgtggcatc tggtgccttc aatggcttag acagaatcat cagtaggagg aaaaatgaaa
     1501 agtatcttgg atttggaaca ccatctaacc tggggaaggg cagaagggct gcagctgctg
     1561 ccgatcttgc taatcgaagt ggagaatcca acactcatca ggacattgaa gagaaagatc
     1621 ggttactccg ccatgtggaa gccagagatc ttattgagtt tggcatgata ccggagtttg
     1681 tgggacggct gcctgttgtg gttcctttgc acagcctaga tgaaaagacg cttgtgcaga
     1741 tactgactga gccacggaat gctgtgattc ctcaatatca ggccttattc agcatggata
     1801 agtgtgaatt gaatgttact gaggatgctt tgaaagccat agctagattg gcactggaaa
     1861 gaaaaacagg tgcacgaggt cttcggtcga taatggaaaa gctgttacta gaaccaatgt
     1921 ttgaagttcc taattctgac attgtatgtg tggaggttga caaagaagta gtagaaggca
     1981 aaaaggaacc aggatacatt cgggctccat cgaaagagtc ctcggaagag gactatgact
     2041 ctggagtgga ggaggacgga tggcctcgcc aagccgatgc tgcaaatagc taagccatca
     2101 gactcccacc tgtgcataca gccttccctc gttctctagg gtcataacag tttctgagtc
     2161 tggcattaaa ggcattgagt ctatcttgga tatcatacat ggtcagcagc ctttaagata
     2221 agaatcagat catgtatatt attgtaacat cacattgatt tatacaaaag acagtatatg
     2281 gactttaatg acacaatgtt tagagataaa aatgtacatt attttggttc tgttttttaa
     2341 aaatatgctt taacaaaatt cttaggaata cttttaagca atgcagatgt tgcaataact
     2401 gtggacttta caataatgtt attctaaaaa tgggaaaata aattctttaa agttga
//
