LOCUS       NM_001389254            9638 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus clock circadian regulator (Clock), transcript
            variant 3, mRNA.
ACCESSION   NM_001389254 XM_017599362
VERSION     NM_001389254.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Xing,C., Zhai,B., Zhang,Y., Fang,Y., Zhang,M., Zhang,C., Wang,W.,
            Ding,M., Huang,X., Shen,B., Wang,R. and Song,L.
  TITLE     Sleep deprivation reduced LPS-induced IgG2b production by
            up-regulating BMAL1 and CLOCK expression
  JOURNAL   Biochem Biophys Res Commun 691, 149326 (2024)
   PUBMED   38035406
  REMARK    GeneRIF: Sleep deprivation reduced LPS-induced IgG2b production by
            up-regulating BMAL1 and CLOCK expression.
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   He,M., Li,L., Li,J., Chen,S. and Shi,H.
  TITLE     rs2253820 Variant Controls Blood Pressure Dip After Stroke by
            Increasing CLOCK-BMAL1 Expression
  JOURNAL   Transl Stroke Res 14 (4), 472-489 (2023)
   PUBMED   35870088
  REMARK    GeneRIF: rs2253820 Variant Controls Blood Pressure Dip After Stroke
            by Increasing CLOCK-BMAL1 Expression.
REFERENCE   3  (bases 1 to 9638)
  AUTHORS   Huang,A., Xiao,G., Chen,Y., Hu,Z., Lee,P.H., Huang,Y., Zhuang,Z.,
            Zhang,Y., Qing,P. and Zhao,C.
  TITLE     Ziwuliuzhu Acupuncture Modulates Clock mRNA, Bmal1 mRNA and
            Melatonin in Insomnia Rats
  JOURNAL   J Acupunct Meridian Stud 16 (3), 109-118 (2023)
   PUBMED   37381033
  REMARK    GeneRIF: Ziwuliuzhu Acupuncture Modulates Clock mRNA, Bmal1 mRNA
            and Melatonin in Insomnia Rats.
REFERENCE   4  (bases 1 to 9638)
  AUTHORS   Tian,L., Li,X., Ding,Y., Li,M., Tang,Y. and Li,D.
  TITLE     The CLOCK protein regulates insulin secretion related with L-type
            calcium channels in rat pancreatic beta cells
  JOURNAL   Biochem Biophys Res Commun 589, 116-122 (2022)
   PUBMED   34906901
  REMARK    GeneRIF: The CLOCK protein regulates insulin secretion related with
            L-type calcium channels in rat pancreatic beta cells.
            Erratum:[Biochem Biophys Res Commun. 2022 Mar 5;595:96. doi:
            10.1016/j.bbrc.2022.01.031.. PMID: 35101272]
REFERENCE   5  (bases 1 to 9638)
  AUTHORS   Saad,L., Kalsbeek,A., Zwiller,J. and Anglard,P.
  TITLE     Rhythmic Regulation of DNA Methylation Factors and Core-Clock Genes
            in Brain Structures Activated by Cocaine or Sucrose: Potential Role
            of Chromatin Remodeling
  JOURNAL   Genes (Basel) 12 (8), 1195 (2021)
   PUBMED   34440369
  REMARK    GeneRIF: Rhythmic Regulation of DNA Methylation Factors and
            Core-Clock Genes in Brain Structures Activated by Cocaine or
            Sucrose: Potential Role of Chromatin Remodeling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9638)
  AUTHORS   Namihira,M., Honma,S., Abe,H., Tanahashi,Y., Ikeda,M. and Honma,K.
  TITLE     Circadian rhythms and light responsiveness of mammalian clock gene,
            Clock and BMAL1, transcripts in the rat retina
  JOURNAL   Neurosci Lett 271 (1), 1-4 (1999)
   PUBMED   10471199
REFERENCE   7  (bases 1 to 9638)
  AUTHORS   Nakagami,K., Uchida,T., Ohwada,S., Koibuchi,Y., Suda,Y., Sekine,T.
            and Morishita,Y.
  TITLE     Increased choline kinase activity and elevated phosphocholine
            levels in human colon cancer
  JOURNAL   Jpn J Cancer Res 90 (4), 419-424 (1999)
   PUBMED   10363580
REFERENCE   8  (bases 1 to 9638)
  AUTHORS   Abe,H., Honma,S., Namihira,M., Tanahashi,Y., Ikeda,M., Yu,W. and
            Honma,K.
  TITLE     Phase-dependent induction by light of rat Clock gene expression in
            the suprachiasmatic nucleus
  JOURNAL   Brain Res Mol Brain Res 66 (1-2), 104-110 (1999)
   PUBMED   10095082
REFERENCE   9  (bases 1 to 9638)
  AUTHORS   Hogenesch,J.B., Gu,Y.Z., Jain,S. and Bradfield,C.A.
  TITLE     The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally
            active complexes with circadian and hypoxia factors
  JOURNAL   Proc Natl Acad Sci U S A 95 (10), 5474-5479 (1998)
   PUBMED   9576906
REFERENCE   10 (bases 1 to 9638)
  AUTHORS   Vitaterna,M.H., King,D.P., Chang,A.M., Kornhauser,J.M.,
            Lowrey,P.L., McDonald,J.D., Dove,W.F., Pinto,L.H., Turek,F.W. and
            Takahashi,J.S.
  TITLE     Mutagenesis and mapping of a mouse gene, Clock, essential for
            circadian behavior
  JOURNAL   Science 264 (5159), 719-725 (1994)
   PUBMED   8171325
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On Dec 5, 2020 this sequence version replaced XM_017599362.1.
            
            Summary: The protein encoded by this gene plays a central role in
            the regulation of circadian rhythms. The protein encodes a
            transcription factor of the basic helix-loop-helix (bHLH) family
            and contains DNA binding histone acetyltransferase activity. The
            encoded protein forms a heterodimer with Arntl (Bmal1) that binds
            E-box enhancer elements upstream of Period (Per1, Per2, Per3) and
            Cryptochrome (Cry1, Cry2) genes and activates transcription of
            these genes. Per and Cry proteins heterodimerize and repress their
            own transcription by interacting in a feedback loop with
            Clock/Arntl complexes. Polymorphisms in this gene may be associated
            with behavioral changes, obesity, and metabolic syndrome. Two
            transcripts encoding different isoforms have been found for this
            gene. [provided by RefSeq, Jan 2014].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643289.34766.1,
                                           SRR26643287.30163.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-138               JAXUCZ010000014.1  32262750-32262887
            139-218             JAXUCZ010000014.1  32264044-32264123
            219-298             JAXUCZ010000014.1  32291269-32291348
            299-388             JAXUCZ010000014.1  32300545-32300634
            389-448             JAXUCZ010000014.1  32301007-32301066
            449-597             JAXUCZ010000014.1  32301330-32301478
            598-689             JAXUCZ010000014.1  32304337-32304428
            690-779             JAXUCZ010000014.1  32309413-32309502
            780-900             JAXUCZ010000014.1  32313750-32313870
            901-1014            JAXUCZ010000014.1  32316563-32316676
            1015-1133           JAXUCZ010000014.1  32316812-32316930
            1134-1216           JAXUCZ010000014.1  32318948-32319030
            1217-1323           JAXUCZ010000014.1  32319222-32319328
            1324-1471           JAXUCZ010000014.1  32320085-32320232
            1472-1547           JAXUCZ010000014.1  32320751-32320826
            1548-1689           JAXUCZ010000014.1  32324199-32324340
            1690-1790           JAXUCZ010000014.1  32325327-32325427
            1791-1880           JAXUCZ010000014.1  32325831-32325920
            1881-2033           JAXUCZ010000014.1  32329247-32329399
            2034-2240           JAXUCZ010000014.1  32330391-32330597
            2241-2446           JAXUCZ010000014.1  32331208-32331413
            2447-2765           JAXUCZ010000014.1  32336419-32336737
            2766-9638           JAXUCZ010000014.1  32340000-32346872
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p11"
     gene            1..9638
                     /gene="Clock"
                     /note="clock circadian regulator"
                     /db_xref="GeneID:60447"
                     /db_xref="RGD:620271"
     exon            1..138
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            139..218
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            219..298
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            299..388
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     CDS             342..2936
                     /gene="Clock"
                     /EC_number="2.3.1.48"
                     /note="isoform 3 is encoded by transcript variant 3;
                     circadian locomoter output cycles protein kaput; rCLOCK;
                     circadian locomoter output cycles kaput"
                     /codon_start=1
                     /product="circadian locomoter output cycles protein kaput
                     isoform 3"
                     /protein_id="NP_001376183.1"
                     /db_xref="GeneID:60447"
                     /db_xref="RGD:620271"
                     /translation="MLFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEK
                     KRRDQFNVLIKELGSMLPGNARKMDKSTVLQKSIDFLRKHKEITAQSDASEIRQDWKP
                     TFLSNEEFTQLMLEALDGFFLAIMTDGSVVYVSETVTSLLEHLPSDLVDQSIFNFIPE
                     GEHSEVYKILSTHLLESDSLTPEDLKSKNQLEFCCHMLRGTIDPKEPSTYEYVRFIGN
                     FKSLNSVSTSTHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEF
                     TSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLESLAKCHEHLMQYGKG
                     KSCYYRFLTKGQQWIWLQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRRELGIE
                     ESLPETAADKSQDSGSDNRINTVSLKEALERFDHSPTPSASSRSSRKSSHTAVSDPSS
                     TPTKIPTDTSTPPRPHLPAHEKMTQRRSSFSSQSINSQSVGSSLTQPAMSQAANLPIP
                     QGMSQFQLSAQLGAMQHLKDQLEQRTRMIEANIHRQQEELRKIQEQLQMVHGQGLQMF
                     LQQSNPGLNFGSVQLSSGNSNIQQLTPINMQGQVVPVNQIQSGVNAGHVSTGQHMIQQ
                     QTLQSTSTQQSQQSVMSGHSQPTSLPNQTPSTLTAPLYNTMVISQPAAGSMVPIPSSM
                     PQNSTQSATVTTFTQDRQIRFSQGQQLVTKLVTAPVACGAVMVPSTMLMGQVVTAYPT
                     FATQQQQAQALSVTQQQQQQQQQQQQQQQQQPQQAQPQPQSQQSSQDQPHPSVQQPAQ
                     LTQPPQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPPSHHQQHQQQQLHRHRTDSLT
                     DPSKVQPQ"
     exon            389..448
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            449..597
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            598..689
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            690..779
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            780..900
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1014
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1133
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1216
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1323
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1471
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1547
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1689
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1790
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..1880
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..2033
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2240
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2446
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2765
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
     exon            2766..9638
                     /gene="Clock"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggagccctg cgcccacgac gcatgcgccg ggagggagcg caatcacgga ctgggctggc
       61 ggctaccggc tggagagagg aaatcccgga cggcgagagc gcgaaggaaa tctggccgcc
      121 gccgcgcgcg cgctcccgtg aacgaaaagc acaagaagaa acttttatag gcattgttga
      181 tttgactagg gcaccgatat cccgaactca ccggaaaggg ttctgattgt cctaagaaga
      241 aggccttaag gttcgtgggt tttggagaat ccgttctgca gacacagaaa gtgaaaagga
      301 gaagtacaaa tgtctaccac aagacgaaaa cataatgtgt tatgttgttt accgtaagct
      361 gtagtaaaat gagctcaatt gttgacagag atgacagtag tatttttgat ggattggtgg
      421 aagaagatga caaggacaaa gcaaaaagag tatctagaaa caaatcagaa aagaaacgta
      481 gagatcagtt caatgtactc attaaggaac tggggtctat gcttcctggt aatgctagaa
      541 agatggacaa gtctactgtt ctacagaaga gcattgattt tttacgcaaa cataaagaga
      601 tcactgcaca gtcagatgct agtgaaattc gacaggactg gaaacctaca ttccttagta
      661 atgaagagtt tacacaatta atgttagagg ctcttgatgg ttttttttta gcgatcatga
      721 cagatggaag tgtagtatat gtatctgaga cggtaacttc gttacttgaa cacttaccat
      781 ctgatcttgt ggatcaaagt atatttaatt ttatcccaga gggagaacat tcagaggttt
      841 ataagatact atctactcac ctgctggaaa gtgactcctt aacccccgag gacttaaaat
      901 caaaaaatca gttagaattc tgttgtcaca tgcttcgagg aacaatagac ccaaaggagc
      961 catccaccta tgaatatgtg agatttatag gaaattttaa atctttaaac agtgtatcaa
     1021 cttcaacaca taatggtttc gaaggaacta tacagcgcac acacaggcct tcttatgaag
     1081 atagagtttg tttcgtagct actgtcagat tagctacccc tcagttcatc aaggaaatgt
     1141 gtactgttga agaaccgaat gaagagttca catctagaca cagtttagaa tggaagtttc
     1201 tatttctaga tcacagggcg ccgccaatca taggctatct gccgtttgaa gtcctgggaa
     1261 catcaggcta tgattactat catgtggacg acctagaaag tctggcaaaa tgtcatgagc
     1321 acttaatgca atatggaaaa ggcaaatcat gttactatag gttcttgacc aaaggacagc
     1381 agtggatttg gcttcagact cattactata tcacttacca tcagtggaac tcaaggccag
     1441 agttcatcgt ttgtactcac actgtagtaa gttatgcaga agttagggct gaaagacggc
     1501 gagaacttgg cattgaggag tctcttcccg agacagctgc tgacaaaagc caagattctg
     1561 ggtctgacaa tcgtataaac acagtgagtc tcaaggaagc actggaaagg ttcgatcaca
     1621 gcccaactcc ttctgcctcc tccagaagct caagaaagtc ctcgcacaca gcagtctcag
     1681 acccttcctc aacgccgaca aagatcccca ctgatactag cactcctccc agaccgcacc
     1741 tgccagctca tgagaagatg acacagcgga ggtcatcctt cagtagtcag tccataaact
     1801 cccagtcagt tggttcatca ttaacacagc cagcgatgtc tcaagctgca aatttaccaa
     1861 tcccacaagg catgtcacag tttcagcttt cagctcagtt aggagccatg cagcatctca
     1921 aagaccagct agagcagcgg acacgcatga tagaagcaaa tattcatcgg cagcaagaag
     1981 aactaaggaa aattcaagaa caacttcaaa tggtccatgg tcaagggcta cagatgtttt
     2041 tgcagcaatc aaaccctgga ctgaattttg gttctgttca actttcctct ggaaattcca
     2101 atatccagca actcacacct ataaatatgc aaggccaagt tgtccctgtt aaccagattc
     2161 agagtggggt gaatgctggc catgtcagca caggccagca catgatccag cagcagactt
     2221 tacaaagtac atcaactcag cagagtcaac agagtgtaat gagtggacac agtcagccaa
     2281 cgtctcttcc aaaccagacg ccgagcactc tgacagcccc actgtacaat acgatggtga
     2341 tctctcagcc tgcagctggg agcatggtcc cgattccatc cagtatgccg cagaacagca
     2401 cccagagtgc tacagtcacc acgttcactc aggacagaca gataaggttt tctcaaggtc
     2461 agcaacttgt gaccaaatta gtgactgctc ctgtagcttg tggggccgtc atggtgccaa
     2521 gtaccatgct tatgggtcag gtagtgactg cctatcctac ctttgccaca caacagcagc
     2581 aggcacaggc cttgtcagta acacagcaac agcagcagca gcagcagcag cagcaacagc
     2641 agcagcagca gcagccgcag caggcgcagc cgcagcccca gtcacagcag agttcccagg
     2701 accagccgca tccttcagtt cagcagccag ctcagctgac ccagccacca caacagttct
     2761 tacagacatc tcggttgctc catgggaatc cttcgacaca gctcatcctc tccgctgcct
     2821 ttccactaca acagagcact ttccctcctt cgcaccacca gcaacaccag caacagcagc
     2881 tccatcggca caggactgac agcctgactg acccttccaa ggtccagcca cagtagcaca
     2941 cgcttcctca gacactggag aggaagggga tggccagaga gactcgctca gatggcatgc
     3001 ggtcagaagt tcaacagttt tgtgagggtc gtcttgagtg ctccagttcc ttgagtagtt
     3061 ggtagggaaa tgctgcccgg cgcttcagaa gtccattata taccagccag tgggaaatgg
     3121 ttatagggac acagccaatt ctgacagttt ctttgcccag gtattttttg atagaaagag
     3181 tatattgcca aatgctaaca agctatcaac catgatcttt actgagtcgg aaaagcacta
     3241 acaatgttgg tagctttaag tgggtctgtg cctgtatcaa atattataga ggacacacca
     3301 ctgccagggg tttgcttcga atgccatgaa gatagtccag tagttagtag tcccacccca
     3361 aactcctccc cctgttccga tagtgatgga accgtgatta cgttgagaat gttgtgcagg
     3421 tttgaattct ctgtgtacag atgctgtaag atgatgtatc gtgtgtctgg atggaggcga
     3481 gaaagcagct ttgtgtgtgc agcgtgccct ccctcacagg taccagtaca ttcattagac
     3541 tcaccaccgt gtgcagactg agaacagctc tcgtctcgtc tcgtttacaa cactgtggag
     3601 ttgtagatct tctccagggc acaagtcttc ctgtgcgtgc tctggttgtg atgtcacagt
     3661 ttgttcatgt gctgctggga atggattgtt tttaaggtta aattattcta catttccatt
     3721 tattcagaaa tatcccttat ttcattattt ttcaattatg cttgagagaa ttgcactgct
     3781 ttattatttt agatggttgg ttgagagttt gatctcatat tttgatatat ttcatagttg
     3841 gaatatttat gtaaatggtt ttcaacaagc ctgaaagtga tttcaagaat gtttcagtta
     3901 taagagtaaa gtttgcacac aaaacatttt aggcactttt ttaacattct cagaggtggg
     3961 aattttaact tttaggattt gttggaatct tttttattat ccttaaaaat ttcaatgctt
     4021 cttttagtca gaaatgattc agggttattt gagggggaaa aaaacccata gtgccttgat
     4081 tttaattcag gtgataattc gccatcttga attcatcgtc tggtttcagc agcagttttg
     4141 aaaccttagt acatttttag cagtgtgtgg tctcagtgtc actctcaagt ccccgtgagg
     4201 acggctgagc ctcttggact accccaacag caccacagaa ttattccctg gtcgcatctt
     4261 cctttaaagc taagtgagtc tttggagaat caccagtaaa tgcgtgctca gagagagggg
     4321 gacacgttgt cttgaaccct caccgcagca ctagaataat gtaactcaca ggcttttaaa
     4381 catacattaa gatttcattt ggcaatatcc ttctctacag gtaataaact ccaacaatgc
     4441 tacatacatt ttaaaagcat ttttttagat tttatggtac taataatgga tttttcaatg
     4501 atgggacaaa agattgtaga aaatagagta tgaagtgcta cagagaaagg gggtcgagtg
     4561 tggcagatga gaattgacct aaaagtgaag tgtgcgatac taaggctgct aaaagaggtg
     4621 ttcagatacc caaggcacag ttgctaggta agggcccggg agctcagagt ccctgtctgt
     4681 cctcagtctc actctcatcc tatgtctttg cttccctgtt cacctggctg caactgagtt
     4741 agaaagtcag tgatgaaaag taaacaacca aagaaaattg gtacctacct tctgcaaaag
     4801 aagtgtggct agataccagt cagtaactca catatcacag agtcctccta gctgacattc
     4861 ttatggacac caggagtagt tgtgagaata cgtgtttatg caggtgtgta cacacatgat
     4921 gacgtcatgt aagtagagca cccacattgc ttttctccct tccaaattgc cttcttctct
     4981 tacgccattc catgtcatct gagttgtgcc tcatttgtct ttttgcattg catagtgata
     5041 agcatttagc tacaggagat acaacacgct agttatgtaa ttcctgttgt tcttcaccat
     5101 tcagcttcct gcttacgagt cgcagttgat aaaggcgctc ctagctgctg acgttgctgt
     5161 ccacaaggct gcacagcagt tgtgtgcacg agctgccgct tagctcctta actcttggtt
     5221 tctttggatg gcaaactgtc tctgtctgct ccccacaaga ctgcagtatt ctgacaaaag
     5281 ttcctcttct gcctgttcat ttctgtagcc aaagctgact gaaaccccaa atctaaatca
     5341 tgaaaagata ccaaatagaa acacttctca gcttcttaga aactttacct tcccctgctc
     5401 tatttcatgg ggattcaagt ttttttgaaa attttgattc tgggcagcgc tttttagtta
     5461 agaaactgtt agggtaaagg ccacacaggt tctggttgtc ctgctgcagg ttccttgcct
     5521 ggactgcttc tacatggggt gacacaggat tagaacagga agcacaaagc tgctcttgag
     5581 gctgccatca aaccagagcc tcgatattga aaagtagtcc tcttttccac cttagaggct
     5641 gcgtgggagg acagcaactg tggcagtggg caggcttgca aatgagcact aaggccttcc
     5701 caagctcaat cctagtcagg gtagcattga gtgctgtgct ctgctgtcac tagacagggc
     5761 ttaggcgtgt actaataaaa gatgccagtg ctttgaatag tttgtgatag aagactccat
     5821 gctaaccagc aagtgtttga attctgtgtg ctgtgtagtt ggtccttgcc ttaaagcagt
     5881 ctcttggaag ttaggagcac tggggcagtc cagccatatg tgggcatcat gttgagggag
     5941 atgagccttg ttcaagcctt agaaaggacc cttagtctac acaggtagat tcttttcact
     6001 tgggtattac tgtgtttaaa atgtgtccac tatgttgagg caatttaagt ggaacacaga
     6061 tagcattttt agtatttctt ttttctgttt ctttggtgat taaaggtttg ttggtaaaca
     6121 tttgtgtaaa agttgttcaa gcctctcatc tttccagtac ctgtggtcct gttgttagaa
     6181 ccagaatcca cgatggaaag tataaacact ggatattaac attgctgagg gcgcatagcc
     6241 aggagcgagc tgactgcaac ttctcagtgg tgaagaaaca gcacaacacg gcacttgcca
     6301 ttttcatagt gattgcatag agaccttcta agattgtctg gattgagtga atactcttct
     6361 aagaggagct tctcaagtaa atgcaaagga aaagagttga ctatttttat agcatattta
     6421 atatatttgt atatatctat gagtgtagta ggaacccttc acaggcctcc cacttttcta
     6481 atcccctccc cttttaccat agcctagtac agcctcatcc gcatgggtaa tgtgcctatc
     6541 gtcagcctac ctaccaaaag atagtgctgc tgctttctga gacaggtgag atcagactct
     6601 catgcctggg gatcctttat gggaggaata gcacacttag aacaacacac cacagtttaa
     6661 aagcatcatt ttgaaaggta ataagcactt tattgcaatt attcatttag ataaagtttg
     6721 tatcttaggc attaaccatt tttaaaggat cccctaatca tcacttaggt gaaattataa
     6781 atgacacatt tctgagaaat gtttagatcc agtgaaccgt agcaggttta tgggagtgat
     6841 ttcaaggtag ccaaataaac tcggacttct gttttgaatg tggtggagtc aggagattgt
     6901 agttgcgtag tttgatttaa acactattgt aaacctatct tgcctattgt gtggacacca
     6961 aaagagacca atgagcctgt ttattttcag aggtctagga atatgcatct gtctgagtag
     7021 atatacagaa ctaatctata aacggttggt agtaatattt taggatacag taatttcaag
     7081 aattattgag tgttttaaat gtgccctgaa atgttggcat gtcatttcag cgttcccatt
     7141 tgagttgctc ttgtaatatt tttgcacaaa aaggactgag aacaagactg ctttggttga
     7201 agaaaactat aatttggtct tattttaatg tctcctgtgg aaacactgga ggtaaattgg
     7261 ttggcatagt tactaattca ggatatttaa aacagtgttg aacagctcat cagaaattaa
     7321 gcaaacttat atatttaaaa attaaaattc tttttttcca cgtgactgtt ttgcatattt
     7381 tttccgttgt cataccacta cattccttgt ttttctgata taatactttg taaaacattc
     7441 aagtattcaa caggactttg taagatgcct gggttctccc aggaagacgt cctagtgaac
     7501 tgagttatag ttgtacagtc tgtgaagact gttgagattc catcaccacc accagcaaac
     7561 actgaggtgc tgagctaagc gacaatcacc attgtaagta tttacaggac gtaggagagg
     7621 ctgcgggaac acttgttccc aaagcagttc tctgggtttc ttacataaaa tggccatgta
     7681 tttgtatctc tacattgtct agttagtgat ttggtttcct gtatttagtt tattgcatca
     7741 ttatcttgtt cttggagaac tggctcttgt ttggggcagt cactacaccc caccctccag
     7801 tgactctcca ctgacagctc attggggtcc attgtacgtc gatggtggtg cgggtgtact
     7861 tccctcctgc tctgaactca tttgaggaaa gaacattaaa agctagcttc tttcagccct
     7921 gctcgcacta ctcagccttt tctagtgtca cttgttgcct tccctgattc atgggcactt
     7981 tttatacggt tgttccccca atagtcaaag gccagtatga cagaaccaag ggcatatgtt
     8041 gcctgttttt gcccatttgg ggaaatgcta gcaaagtaat atctattaat ggctgccagt
     8101 catccttata tccatacata aatgtcctta tatatatagt ttaacaatca atgtatgcac
     8161 actgaaaact ttgaaaagtt ggctatcttt agatcctcca tagtccagca aagtggtgct
     8221 taaacatggg aagccgagct gaggggctcc ttcctgctca cacacactgg gaaatgacct
     8281 ccttcagcac taccgtggct ctggagcccc acaggcagag tctagcacac acccctggag
     8341 gacagttacg aaaatactgt cagacacaaa atgggtttaa tgacctttaa ggcatttaac
     8401 tctgaggggg gtaaattcaa aatataccat tttttgatgt ttggttttaa atctgagaaa
     8461 acaaactgtg acatcgcttt aagaaatgac atccaaatgt tttatgtttg agttggcctg
     8521 tactggttca catttattgc tggcaatggt tttaagagta ttacaaagct aatatacaga
     8581 ggaaactgtt aagttgtata actagacaat cagcttcaag caaagacatt gccttcaaaa
     8641 agtccattat taagcaatgg gtgtgtcagc cagaactgga atttatacct ttctaaaatg
     8701 attttaaatc tattttcctg tggtttaagt tggggaaagc tccagtatag cactttcata
     8761 tttaccctgt gtaaagatta aagcccaaag acaaattagt gctgtattaa aaccagttag
     8821 tgcaaaaaaa aaatatatgt tttatctttg taaagtttaa ttgttaaatt ggttagtagt
     8881 tgtacaaaca tgcaccaggt ttttctaagt tccgtgtgtc ttgctgtgct gttcttgaca
     8941 ttgctgtcct tactgcttgg ttctgggttt aaactgaaac aataatagac agagcccatc
     9001 gtttctggct gccgatctcg gataagcatt cttcccctcc taagcaaaca tgtgattgaa
     9061 ataagcttaa ttcatagaac tgagactgac gttctttcag gtatcaccac tttgccaaaa
     9121 ccagaactat gaaggattag gaatggtttc aatggtgcta cattaggttc cctggttttc
     9181 ataagctacg tttggaaata cagtattgaa taaaatgtag actcttgtta acagctgaat
     9241 attgccattt taattgggtc caatataatt ggttattctt atcaaagtta acctttggag
     9301 aagtaaaact gttacatcag cacgcctcac tcaggcttat gctgcatgac gggcacagct
     9361 ctaacttagt ctcaacatgc catctaacac tctcttctct agaccttcga gtgtattcag
     9421 aacctaaagc tacagcagaa gagccaagca gtagcaatgt ttgttttatt tatttttttt
     9481 gtaaatgtaa aaataactag atccagacct gtttcctttg atttaaaata aagaatacct
     9541 cagtgctgct tctcagaatc acctgcttca tgtggcacat aatgtgtaag agtcccattt
     9601 ggatttacaa agactattaa atgtttttaa atctgtca
//
