LOCUS       NM_031818               4056 bp    mRNA    linear   ROD 05-AUG-2024
DEFINITION  Rattus norvegicus chloride intracellular channel 4 (Clic4), mRNA.
ACCESSION   NM_031818
VERSION     NM_031818.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Yin,X., Zhao,J., Jiang,H., Li,L., Jiang,J., Xi,H., Peng,X., Yin,X.,
            Shi,X. and Zhang,L.
  TITLE     Impact of Xenon on CLIC4 and Bcl-2 Expression in Lipopolysaccharide
            and Hypoxia-Ischemia-Induced Periventricular White Matter Damage
  JOURNAL   Neonatology 113 (4), 339-346 (2018)
   PUBMED   29518790
  REMARK    GeneRIF: Xenon decreased neuronal apoptosis via Bcl-2- and
            CLIC4-mediated pathways in white matter damage
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Hatziioanou,D., Barkas,G., Critselis,E., Zoidakis,J.,
            Gakiopoulou,H., Androutsou,M.E., Drossopoulou,G., Charonis,A. and
            Vlahakos,D.V.
  TITLE     Chloride Intracellular Channel 4 Overexpression in the Proximal
            Tubules of Kidneys from the Spontaneously Hypertensive Rat: Insight
            from Proteomic Analysis
  JOURNAL   Nephron 138 (1), 60-70 (2018)
   PUBMED   29131056
  REMARK    GeneRIF: overexpression of CLIC4 suggests that alterations in the
            proximal tubular compartment during hypertension should be further
            examined and that CLIC4 may be a useful early marker of renal
            tubular alterations due to elevated blood pressure
REFERENCE   3  (bases 1 to 4056)
  AUTHORS   Ponnalagu,D., Gururaja Rao,S., Farber,J., Xin,W., Hussain,A.T.,
            Shah,K., Tanda,S., Berryman,M., Edwards,J.C. and Singh,H.
  TITLE     Molecular identity of cardiac mitochondrial chloride intracellular
            channel proteins
  JOURNAL   Mitochondrion 27, 6-14 (2016)
   PUBMED   26777142
  REMARK    GeneRIF: The authors report the presence of unique dimorphic
            chloride intracellular channel (CLIC) proteins namely CLIC1, CLIC4
            and CLIC5 as abundant CLICs in the rodent heart. Further, CLIC4,
            CLIC5, and an ortholog present in Drosophila (DmCLIC) localize to
            adult cardiac mitochondria.
REFERENCE   4  (bases 1 to 4056)
  AUTHORS   Wojciak-Stothard,B., Abdul-Salam,V.B., Lao,K.H., Tsang,H.,
            Irwin,D.C., Lisk,C., Loomis,Z., Stenmark,K.R., Edwards,J.C.,
            Yuspa,S.H., Howard,L.S., Edwards,R.J., Rhodes,C.J., Gibbs,J.S.,
            Wharton,J., Zhao,L. and Wilkins,M.R.
  TITLE     Aberrant chloride intracellular channel 4 expression contributes to
            endothelial dysfunction in pulmonary arterial hypertension
  JOURNAL   Circulation 129 (17), 1770-1780 (2014)
   PUBMED   24503951
  REMARK    GeneRIF: Increased CLIC4 expression is an early manifestation and
            mediator of endothelial dysfunction in pulmonary hypertension.
REFERENCE   5  (bases 1 to 4056)
  AUTHORS   Chuang,J.Z., Chou,S.Y. and Sung,C.H.
  TITLE     Chloride intracellular channel 4 is critical for the epithelial
            morphogenesis of RPE cells and retinal attachment
  JOURNAL   Mol Biol Cell 21 (17), 3017-3028 (2010)
   PUBMED   20610659
  REMARK    GeneRIF: CLIC4 plays an important role in retinal pigment
            epithelium-photoreceptor adhesion, perhaps by modulating the
            activity of cell surface channels/transporters.
REFERENCE   6  (bases 1 to 4056)
  AUTHORS   Suginta,W., Karoulias,N., Aitken,A. and Ashley,R.H.
  TITLE     Chloride intracellular channel protein CLIC4 (p64H1) binds directly
            to brain dynamin I in a complex containing actin, tubulin and
            14-3-3 isoforms
  JOURNAL   Biochem J 359 (Pt 1), 55-64 (2001)
   PUBMED   11563969
REFERENCE   7  (bases 1 to 4056)
  AUTHORS   Berryman,M. and Bretscher,A.
  TITLE     Identification of a novel member of the chloride intracellular
            channel gene family (CLIC5) that associates with the actin
            cytoskeleton of placental microvilli
  JOURNAL   Mol Biol Cell 11 (5), 1509-1521 (2000)
   PUBMED   10793131
REFERENCE   8  (bases 1 to 4056)
  AUTHORS   Fernandez-Salas,E., Sagar,M., Cheng,C., Yuspa,S.H. and
            Weinberg,W.C.
  TITLE     p53 and tumor necrosis factor alpha regulate the expression of a
            mitochondrial chloride channel protein
  JOURNAL   J Biol Chem 274 (51), 36488-36497 (1999)
   PUBMED   10593946
REFERENCE   9  (bases 1 to 4056)
  AUTHORS   Kloeker,S. and Wadzinski,B.E.
  TITLE     Purification and identification of a novel subunit of protein
            serine/threonine phosphatase 4
  JOURNAL   J Biol Chem 274 (9), 5339-5347 (1999)
   PUBMED   10026142
REFERENCE   10 (bases 1 to 4056)
  AUTHORS   Duncan,R.R., Westwood,P.K., Boyd,A. and Ashley,R.H.
  TITLE     Rat brain p64H1, expression of a new member of the p64 chloride
            channel protein family in endoplasmic reticulum
  JOURNAL   J Biol Chem 272 (38), 23880-23886 (1997)
   PUBMED   9295337
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Sep 5, 2022 this sequence version replaced NM_031818.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ222737.1, SRR26643286.18222.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676807 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-249               JAXUCZ010000005.1  152796870-152797118 c
            250-359             JAXUCZ010000005.1  152764434-152764543 c
            360-485             JAXUCZ010000005.1  152751264-152751389 c
            486-592             JAXUCZ010000005.1  152746258-152746364 c
            593-774             JAXUCZ010000005.1  152741565-152741746 c
            775-4056            JAXUCZ010000005.1  152737371-152740652 c
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..4056
                     /gene="Clic4"
                     /note="chloride intracellular channel 4"
                     /db_xref="GeneID:83718"
                     /db_xref="RGD:61857"
     exon            1..249
                     /gene="Clic4"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..939
                     /gene="Clic4"
                     /note="mitochondrial chloride intracellular channel 4;
                     glutaredoxin-like oxidoreductase CLIC4; chloride
                     intracellular channel 4 (mitochondrial); intracellular
                     chloride ion channel protein p64H1"
                     /codon_start=1
                     /product="chloride intracellular channel protein 4"
                     /protein_id="NP_114006.2"
                     /db_xref="GeneID:83718"
                     /db_xref="RGD:61857"
                     /translation="MALSMPLNGLKEEDKEPLIELFVKAGSDGESIGNCPFSQRLFMI
                     LWLKGVVFSVTTVDLKRKPADLQNLAPGTHPPFITFNSEVKTDVNKIEEFLEEVLCPP
                     KYLKLSPKHPESNTAGMDIFAKFSAYIKNSRPEANEALERGLLKTLQKLDEYLNSPLP
                     DEIDENSMEDIKSSTRRFLDGDEMTLADCNLLPKLHIVKVVAKKYRNFDIPKGMTGIW
                     RYLTNAYSRDEFTNTCPSDKEVEIAYSDVAKRLTK"
     exon            250..359
                     /gene="Clic4"
                     /inference="alignment:Splign:2.1.0"
     exon            360..485
                     /gene="Clic4"
                     /inference="alignment:Splign:2.1.0"
     exon            486..592
                     /gene="Clic4"
                     /inference="alignment:Splign:2.1.0"
     exon            593..774
                     /gene="Clic4"
                     /inference="alignment:Splign:2.1.0"
     exon            775..4056
                     /gene="Clic4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcgcgcgcg aggcgtgagg cggtgctgag gatctgagcc acgggtagct agacgcccgg
       61 actgtccagt gagcgagcga gccagcgagc cagcgagcca gaggcaggaa cccgcagcca
      121 ctgcagcccc ggagccacag ccgcagccgc cgtccgcgga gcccagccca actggccatg
      181 gcgctgtcga tgcccctgaa cggactgaag gaggaggaca aagagcccct catcgagctc
      241 ttcgtcaagg ccggcagtga tggtgagagc ataggaaact gccccttttc acagaggctc
      301 ttcatgatcc tttggctcaa aggagtcgtg ttcagtgtca ccaccgttga cctgaaaagg
      361 aagcctgcag atctgcagaa cttggctcct gggacccacc caccatttat aaccttcaac
      421 agcgaagtca aaacggatgt aaataagatt gaggaatttc ttgaagaagt cttgtgccca
      481 cccaagtact taaagctctc accaaaacac ccagagtcaa acactgctgg gatggacatc
      541 tttgccaagt tctctgcgta catcaagaac tcaagaccag aggctaatga agcactggag
      601 agggggctct tgaaaacgct gcagaagctg gatgagtacc tgaattctcc cctccctgac
      661 gaaatcgatg agaacagcat ggaggacatc aaatcgtcca cacgcaggtt tctggacggc
      721 gacgagatga cattagcaga ctgcaacctg ctgcccaagc ttcacattgt caaggtggtg
      781 gccaaaaaat accgcaactt tgacattccc aaaggcatga cgggcatctg gagatacctg
      841 acaaacgcct acagtaggga tgagttcacc aacacctgtc ccagcgacaa ggaggtggaa
      901 atcgcgtaca gtgacgtcgc caagagactt accaagtaga cagcactttt gaaagcaatg
      961 ccttcagagt cccccaagac tatgctttcc taacagacgg ctcctgcctc ctctaagcag
     1021 acgtgtattt tgcatgaaca cacagttact cgagactagg attaagggta gacaaagtag
     1081 gtctctccag cgcacactcc taagcagtat tattctaaac tcttctaccc agcccacccg
     1141 cccttccctg cccatccgct ggcctccact ttggagacac tttgccacaa gcctgtcacc
     1201 taagaagcag tttgctgtca ctctggagcc ccagccatca cgtccattca ctctgtagag
     1261 ggcagaactg gaggtgcagc gttcggccat tagaatagta gctgcgacat cagtggaccc
     1321 cacgctgctg tccatgcatg gcacaggaat gtttatgtag tgggagttgt aatattgagt
     1381 aaggaatctg gaaagcttgc taccgggtta atatgctgtt tcagcctagt ttgccctggg
     1441 gtatctaaga ctgcttctgc agagacgctg gtttcttagt agacacttaa ttttgcctaa
     1501 aatgtgtcat ttgtataaat tgtaacatcc aatactacat agaaagtgaa gggctgaggg
     1561 ttgggaattc attttcaaat gatgagatga ttggctgggt tctctgtcta ccttacctgc
     1621 tgtgaggggc tctcctaggg ccgctcttac atttacagct gccttctttg tgacagactt
     1681 tttcttttct cttttttgtg gtgcttggga tgggacccag ggctttgtgc aggcctggta
     1741 aggctctgtc cctgggctgc accgagcccc acctctctat gggtgaccgt atgtaatcct
     1801 gacaaagacc agcatgtgag ccactcagaa gttagccagg atcgggggag tttgccgaag
     1861 tgctacgtac actttactgt ggagaggggt gaagagtgtg ccaccttaaa ctccgtgacg
     1921 ctcaggctgc cacgggctcc tgtcccacct ccatttgcat cagaccgagt gaggggtcct
     1981 tcacttctct ggggataata gtggttgtca tagtgagtgt gtattttcca gttttgcttg
     2041 tcagtccccg caaagaagag gaagcctctg atttcttgct gtttggtttg agacaggatg
     2101 ttactctgga gccctggttg acctggagcg tcataagtag accacgctgg tctcaaactt
     2161 atggcacctc tcctgccttc gcctcctgca tgctggtgag gattatgggc ctgcacacca
     2221 tgtccgactt tttttttttt tcttcatttt ttaaaacagt ctaaactaag ttgtttgcag
     2281 tttaacaaat gagaaatgag ctttgtaaaa atagaggcca ctcccaccca aacaaaatgg
     2341 cctttctcag tagtccccca gagcctgctt gtgactgtct atatttgcta catttttttg
     2401 gtgatttttt ttttcttttt tgaagcatgt caggaagtgc tctgctcttt cttcgacttc
     2461 tccctcccca gttggctgct ttaaggtgcc tatgctttct ttccctttgg tgtgctgaca
     2521 cttaataaac gcctgcttta tggaagcatt ctgactcata agcagctccc tttaaaactt
     2581 aacaataatt ccttagcgcg ggtgtcagga aaagcttgac agtggcccct gtggctcagg
     2641 gagactcctt ggccgcaggc ttcacccttc ctgctgatct gggtgtgagc cccccatcac
     2701 ggtgcaaact gggtggtggt ctgcgttcca atgggaggca ctatcttttc tccttggaga
     2761 agtgaccctg cagtgtctct gtgggtggaa gtgtcccaac tttgcttggt gatgacgtgg
     2821 ccgtgggtgg ggttgagtct agaggtgtgt ctgttctttt tgtgagtggg ccaagcgatg
     2881 gctgctgtcc tcctcgcaac ggaggatatg caggacggag aagaggacgt gctttagtgg
     2941 gtgtgagatg cccacgcttg agtgtttatt ctctgtggct tcctaaattg actctcggtt
     3001 gttcttacta tctctggata tccaagggaa gtctgtctag gttttgcaaa ggcagaattg
     3061 tttttcttta tagagaggga cagttttata tcaagaaagt caaacagatt tgcgtttggc
     3121 agatggacag aagctataaa agaaagaccc cgcggcaagc accctgcact ctgcgaggtg
     3181 gtcatggctt acaatcttga catgggagat gtgagtgttt ccatgtggct gatgccgtgc
     3241 acgggctgac ggacgtccat gcgagtggct ccccctctgc tcttgtctaa cctccttttt
     3301 aagccctctg tcttggagca gggattctga acggaggctc cctattttct agtggatact
     3361 aagaaactat taagcagcgg gacgcctcgc agttttaagt atataacttc ctttttgata
     3421 tagtacagac tttgaataca atacaatttt cttggtaaca ttagctttca tagctctaga
     3481 ctcatgagca gtttatactg ttgagaggtt ctgactggtt tgtgcacagg cggaccggct
     3541 ctagaatcta ggcagttata cttccagaac ctctgtgttg catgtagata gcatttattt
     3601 ctgcatattt aaaaaaccca tttaggaaat aagaaaaaaa tgagaagtag aaattatttt
     3661 ttcatgaaga ttttggtcca cacctcactg tgttgtaacg gattcaccag tttcttgagc
     3721 tggcaataat caacatttgg ttcagataga gagagagaga gagagcaaga gagcatttgt
     3781 tagcaaaact cacagacttc tgaaagttca ttgttgtaat gatcaaagtg ggggaaacaa
     3841 attattttat caaggaaaaa aagttgagtc ccaagccata aaagttggac tcccaggcgg
     3901 taatgctgga cagccttgga gcatttcatg ggattccttg ggtaggaatg aaagctcttt
     3961 acaaaatgct tgcattttct tctctctgga acatcaacac cagtatattg ctggcagcta
     4021 ttgtattaaa aaaataaata tattttcact atcaaa
//
