LOCUS       NM_001109472            2274 bp    mRNA    linear   ROD 14-NOV-2024
DEFINITION  Rattus norvegicus calmegin (Clgn), transcript variant 1, mRNA.
ACCESSION   NM_001109472 XM_001063487
VERSION     NM_001109472.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Itcho,K., Oki,K., Gomez-Sanchez,C.E., Gomez-Sanchez,E.P., Ohno,H.,
            Kobuke,K., Nagano,G., Yoshii,Y., Baba,R., Hattori,N. and Yoneda,M.
  TITLE     Endoplasmic Reticulum Chaperone Calmegin Is Upregulated in
            Aldosterone-Producing Adenoma and Associates With Aldosterone
            Production
  JOURNAL   Hypertension 75 (2), 492-499 (2020)
   PUBMED   31865789
  REMARK    GeneRIF: Endoplasmic Reticulum Chaperone Calmegin Is Upregulated in
            Aldosterone-Producing Adenoma and Associates With Aldosterone
            Production.
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Yamaguchi,R., Fujihara,Y., Ikawa,M. and Okabe,M.
  TITLE     Mice expressing aberrant sperm-specific protein PMIS2 produce
            normal-looking but fertilization-incompetent spermatozoa
  JOURNAL   Mol Biol Cell 23 (14), 2671-2679 (2012)
   PUBMED   22621904
REFERENCE   3  (bases 1 to 2274)
  AUTHORS   Tokuhiro,K., Ikawa,M., Benham,A.M. and Okabe,M.
  TITLE     Protein disulfide isomerase homolog PDILT is required for quality
            control of sperm membrane protein ADAM3 and male fertility
            [corrected]
  JOURNAL   Proc Natl Acad Sci U S A 109 (10), 3850-3855 (2012)
   PUBMED   22357757
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5905]
REFERENCE   4  (bases 1 to 2274)
  AUTHORS   Ikawa,M., Nakanishi,T., Yamada,S., Wada,I., Kominami,K., Tanaka,H.,
            Nozaki,M., Nishimune,Y. and Okabe,M.
  TITLE     Calmegin is required for fertilin alpha/beta heterodimerization and
            sperm fertility
  JOURNAL   Dev Biol 240 (1), 254-261 (2001)
   PUBMED   11784061
REFERENCE   5  (bases 1 to 2274)
  AUTHORS   Yoshinaga,K., Tanii,I. and Toshimori,K.
  TITLE     Molecular chaperone calmegin localization to the endoplasmic
            reticulum of meiotic and post-meiotic germ cells in the mouse
            testis
  JOURNAL   Arch Histol Cytol 62 (3), 283-293 (1999)
   PUBMED   10495883
REFERENCE   6  (bases 1 to 2274)
  AUTHORS   Ohsako,S., Janulis,L., Hayashi,Y. and Bunick,D.
  TITLE     Characterization of domains in mice of calnexin-t, a putative
            molecular chaperone required in sperm fertility, with use of
            glutathione S-transferase-fusion proteins
  JOURNAL   Biol Reprod 59 (5), 1214-1223 (1998)
   PUBMED   9780330
REFERENCE   7  (bases 1 to 2274)
  AUTHORS   Ikawa,M., Wada,I., Kominami,K., Watanabe,D., Toshimori,K.,
            Nishimune,Y. and Okabe,M.
  TITLE     The putative chaperone calmegin is required for sperm fertility
  JOURNAL   Nature 387 (6633), 607-611 (1997)
   PUBMED   9177349
REFERENCE   8  (bases 1 to 2274)
  AUTHORS   Ohsako,S., Hayashi,Y. and Bunick,D.
  TITLE     Molecular cloning and sequencing of calnexin-t. An abundant male
            germ cell-specific calcium-binding protein of the endoplasmic
            reticulum
  JOURNAL   J Biol Chem 269 (19), 14140-14148 (1994)
   PUBMED   8188695
REFERENCE   9  (bases 1 to 2274)
  AUTHORS   Watanabe,D., Yamada,K., Nishina,Y., Tajima,Y., Koshimizu,U.,
            Nagata,A. and Nishimune,Y.
  TITLE     Molecular cloning of a novel Ca(2+)-binding protein (calmegin)
            specifically expressed during male meiotic germ cell development
  JOURNAL   J Biol Chem 269 (10), 7744-7749 (1994)
   PUBMED   8126001
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473972.1.
            
            On Oct 4, 2007 this sequence version replaced XM_001063487.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760383,
                              SAMEA5760434 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q11"
     gene            1..2274
                     /gene="Clgn"
                     /note="calmegin"
                     /db_xref="GeneID:685504"
                     /db_xref="RGD:1592643"
     exon            1..215
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            216..365
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     CDS             225..2060
                     /gene="Clgn"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /codon_start=1
                     /product="calmegin isoform 1 precursor"
                     /protein_id="NP_001102942.1"
                     /db_xref="GeneID:685504"
                     /db_xref="RGD:1592643"
                     /translation="MHFQGVGLCLGLLFITAKADFVDDGVEVEDFSESSDESDFKDEP
                     SSGTIKYKTPQPIGEVYFTETFDSGNLSGWVLSKAKKDDMDSEISIYDGRWEIEELKE
                     NQVPGDRGLVLKSKAKHHAISAVLEKPFIFADKPLIVQYEVNFQDGIDCGGAYVKLLA
                     DTGDLILENFYDKTSYTIMFGPDKCGEDYKLHLIFRHKHPKTGVFEEKHAKPPDVDLK
                     EFFTDRKTHLYTLVMNPDDTFEILIDQKVVNQGTLLEDVVPPINPPREIDDPNDRKPE
                     EWDDRAKIPDPTAVKPEDWDEKEPIQIEDPSAVKPDGWLDDEPKFIPNPKAEKPEDWS
                     DSMDGEWEAPHIPNPACQIGCGEWKPPMIDNPKYKGIWRPPMINNPNYQGIWSPQKIP
                     NPDYFEDDHPFLLTSFSALGLELWSMTPDIYFDNFIICSEKEVADQWAADGWELKIMV
                     ANANEPGVLRQLTTAAEERPWLWLMYLVMAGLPIALVTSFCWPRKVKKKYEDIGPKKA
                     DLCQLPSKAALEQEPEEEKAPEKPEDLQEEKKPSETEVVIVEKEVIGEPEEKSEEDAE
                     TIEGQEEMTKLSKSGSEDEMKDADESTGSGDAPVKSLRKRRVRKD"
     exon            366..442
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            443..501
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            502..643
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            644..725
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            726..918
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1108
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1222
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1373
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1589
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1715
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1878
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..1979
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2274
                     /gene="Clgn"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcggctgcc ggcgggatcg gtctggggac gcgcccggca ccgagtagcg gccagtcgga
       61 cacctgagct gccgctgctg gacacaagcc cgcaggacag gtgtgtagac cgcgggccgc
      121 cttcctgcgg cttgctctct gggagcgggg tgagaaatcg gatccaggtg cccggcagca
      181 tcctcttctg agactagaag gctcagcttg gtgcggtagt caatatgcat ttccagggtg
      241 ttggactgtg cttgggtctc ctgttcatca cggccaaggc ggattttgtg gatgatggtg
      301 ttgaggtgga agacttcagt gagagttcag acgagagtga ttttaaagac gaaccttcct
      361 cggggactat taaatataag acacctcaac ctataggaga agtatatttt acagaaacat
      421 ttgatagtgg aaatctgtct gggtgggtct tatcaaaagc aaagaaggat gatatggatt
      481 cagaaatttc tatatatgat ggaagatggg aaatagagga gctgaaggaa aaccaggtgc
      541 ctggtgacag agggctggtt ctgaagtcta aggccaagca ccatgcgata tctgctgtcc
      601 tcgagaaacc ctttattttt gccgataaac ctttgatagt tcaatatgaa gtaaattttc
      661 aagatggtat tgactgtgga ggcgcctacg ttaagctgct agcagacact ggtgacttga
      721 ttctggaaaa cttttatgat aaaacatcgt ataccatcat gtttggacca gataaatgtg
      781 gagaagacta taagcttcat ttaatcttca gacacaaaca tcccaaaacc ggagtttttg
      841 aagaaaagca cgccaaacct ccagatgtgg atctgaaaga gttctttacg gaccggaaga
      901 ctcacctgta taccctcgtg atgaatccgg atgacacgtt tgaaatatta atcgatcaga
      961 aagttgtgaa ccaagggacc ttgctagagg acgtggtccc acctatcaat ccacccagag
     1021 aaattgatga cccgaatgac agaaagccag aggagtggga tgacagagcc aagatccccg
     1081 accccaccgc cgtcaagcca gaggactggg atgaaaagga acccatacaa atcgaagacc
     1141 cgagtgctgt taagcctgac ggctggctcg atgatgaacc aaaatttatc cccaatccta
     1201 aagctgagaa acccgaagac tggagtgaca gcatggatgg agaatgggag gcgccacaca
     1261 ttcctaaccc agcctgtcag atcggatgtg gagaatggaa acctcccatg atagataacc
     1321 caaaatacaa agggatctgg agaccaccga tgatcaataa ccctaactac cagggaatct
     1381 ggagtcctca gaaaattcct aacccggatt attttgaaga tgaccaccca ttccttctga
     1441 cttctttcag tgctctgggc ttagagcttt ggtccatgac tcctgacatc tactttgata
     1501 attttatcat ctgttcagaa aaggaggtgg cggatcagtg ggctgcagat ggctgggagc
     1561 tgaagataat ggtggcaaat gccaatgagc ccggcgtcct ccgacagtta acaacagcag
     1621 cagaggaacg cccctggctc tggctcatgt acctggtgat ggctgggttg cccatcgctt
     1681 tagttacttc attttgttgg ccaagaaaag taaagaaaaa atatgaagac atagggccta
     1741 aaaaagctga cttatgtcaa ctgccaagca aggccgctct ggaacaggaa cctgaggagg
     1801 agaaggcccc agagaagccc gaagatttgc aagaggaaaa gaagccaagt gaaactgaag
     1861 ttgtcatcgt ggaaaaggaa gtaattggag aaccggaaga gaagagtgag gaagacgcag
     1921 aaactataga agggcaagag gaaatgacca agttgagtaa atctggatca gaggatgaga
     1981 tgaaggatgc tgatgagagc acaggatctg gagacgcccc agtgaagtca ttgcgcaaga
     2041 ggagggtacg aaaggactga cacacgcacg tcaagtgttg caaatcccaa agcgagatct
     2101 ggcatcgaga gttaggatgc ccaaaaggac agtgaccgcg catctgcaca aactctaagt
     2161 atctagcaat gttactttca ttctttcagg gtggggggaa ggggatgcag gggaagtaac
     2221 tttgaagtta acaggtcttt ggatttagaa taaaaagaaa aatggcacct caca
//
