LOCUS       NM_173103               2313 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Rattus norvegicus chloride voltage-gated channel Kb (Clcnkb), mRNA.
ACCESSION   NM_173103
VERSION     NM_173103.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Luan,H., Wu,P., Wang,M., Sui,H., Fan,L. and Gu,R.
  TITLE     Effects of adenosine stimulation on the mRNA expression of CLCNKB
            in the basolateral medullary thick ascending limb of the rat kidney
  JOURNAL   Mol Med Rep 14 (5), 4391-4398 (2016)
   PUBMED   27748841
  REMARK    GeneRIF: The present study showed that adenosine affected the mRNA
            expression of CLCNKB, initially through the cAMPPKA pathway and
            then the PLA2AA pathway.
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Capasso,G., Rizzo,M., Garavaglia,M.L., Trepiccione,F., Zacchia,M.,
            Mugione,A., Ferrari,P., Paulmichl,M., Lang,F., Loffing,J.,
            Carrel,M., Damiano,S., Wagner,C.A., Bianchi,G. and Meyer,G.
  TITLE     Upregulation of apical sodium-chloride cotransporter and
            basolateral chloride channels is responsible for the maintenance of
            salt-sensitive hypertension
  JOURNAL   Am J Physiol Renal Physiol 295 (2), F556-F567 (2008)
   PUBMED   18480177
  REMARK    GeneRIF: In salt-sensitive hypertension, there is a strong
            upregulation, both of NCC and ClC-K along the distal convoluted
            tubules, which explains the persistence of hypertension.
REFERENCE   3  (bases 1 to 2313)
  AUTHORS   Speirs,H.J., Wang,W.Y., Benjafield,A.V. and Morris,B.J.
  TITLE     No association with hypertension of CLCNKB and TNFRSF1B
            polymorphisms at a hypertension locus on chromosome 1p36
  JOURNAL   J Hypertens 23 (8), 1491-1496 (2005)
   PUBMED   16003175
REFERENCE   4  (bases 1 to 2313)
  AUTHORS   Jeck,N., Waldegger,S., Lampert,A., Boehmer,C., Waldegger,P.,
            Lang,P.A., Wissinger,B., Friedrich,B., Risler,T., Moehle,R.,
            Lang,U.E., Zill,P., Bondy,B., Schaeffeler,E., Asante-Poku,S.,
            Seyberth,H., Schwab,M. and Lang,F.
  TITLE     Activating mutation of the renal epithelial chloride channel ClC-Kb
            predisposing to hypertension
  JOURNAL   Hypertension 43 (6), 1175-1181 (2004)
   PUBMED   15148291
REFERENCE   5  (bases 1 to 2313)
  AUTHORS   Hayama,A., Rai,T., Sasaki,S. and Uchida,S.
  TITLE     Molecular mechanisms of Bartter syndrome caused by mutations in the
            BSND gene
  JOURNAL   Histochem Cell Biol 119 (6), 485-493 (2003)
   PUBMED   12761627
REFERENCE   6  (bases 1 to 2313)
  AUTHORS   Waldegger,S., Jeck,N., Barth,P., Peters,M., Vitzthum,H., Wolf,K.,
            Kurtz,A., Konrad,M. and Seyberth,H.W.
  TITLE     Barttin increases surface expression and changes current properties
            of ClC-K channels
  JOURNAL   Pflugers Arch 444 (3), 411-418 (2002)
   PUBMED   12111250
REFERENCE   7  (bases 1 to 2313)
  AUTHORS   Jeck,N., Konrad,M., Peters,M., Weber,S., Bonzel,K.E. and
            Seyberth,H.W.
  TITLE     Mutations in the chloride channel gene, CLCNKB, leading to a mixed
            Bartter-Gitelman phenotype
  JOURNAL   Pediatr Res 48 (6), 754-758 (2000)
   PUBMED   11102542
REFERENCE   8  (bases 1 to 2313)
  AUTHORS   Kieferle,S., Fong,P., Bens,M., Vandewalle,A. and Jentsch,T.J.
  TITLE     Two highly homologous members of the ClC chloride channel family in
            both rat and human kidney
  JOURNAL   Proc Natl Acad Sci U S A 91 (15), 6943-6947 (1994)
   PUBMED   8041726
REFERENCE   9  (bases 1 to 2313)
  AUTHORS   Adachi,S., Uchida,S., Ito,H., Hata,M., Hiroe,M., Marumo,F. and
            Sasaki,S.
  TITLE     Two isoforms of a chloride channel predominantly expressed in thick
            ascending limb of Henle's loop and collecting ducts of rat kidney
  JOURNAL   J Biol Chem 269 (26), 17677-17683 (1994)
   PUBMED   8021279
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from Z30663.1.
            
            Summary: This gene is a member of the CLC family of voltage-gated
            chloride channels. The gene is located adjacent to a highly similar
            chloride channel gene on chromosome 5. This gene is syntenic with
            human CLCNKA (geneID:1187).[provided by RefSeq, Sep 2009].
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z30663.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132263,
                                           SAMD00132278 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..2313
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /note="chloride voltage-gated channel Kb"
                     /db_xref="GeneID:79430"
                     /db_xref="RGD:628639"
     exon            1..13
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            14..120
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..2084
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /note="chloride channel K2; chloride channel,
                     voltage-sensitive Kb; clC-K2; chloride channel Kb"
                     /codon_start=1
                     /product="chloride channel protein ClC-Kb"
                     /protein_id="NP_775126.1"
                     /db_xref="GeneID:79430"
                     /db_xref="RGD:628639"
                     /translation="MEEIVGLREGSPRKPVPLQELWRPCPRIRRNIQGSLEWLKERLF
                     RVGEDWYFLVALGVLMALISYAMNFAIGRVVRAHKWLYREIGDGHLLRYLSWTVYPVA
                     LLSFSSGFSQSITPSSGGSGIPEVKTILTGVILEDYLDIKNFGAKVVGLSCTLATGST
                     IFLGKLGPFVHLSVMIAAYLGRVRTKTVGEPENKTKEMELLAAGAAVGVATVFAAPIS
                     GVLFSIEVMSSHFSVWDYWRGFFAATCGAFMFHLLAVFNSEQETITSIYKTSFPVDIP
                     FDLPEIFFFVALGAICGILSCGYNYCQRTSLFFLKSNGFTSKLLATSKPLYSALAAVV
                     LASITYPPGVGRFMASRLSMSEYLETLFDNNSWALMTKNSSPPWSAEPDPQNLWLEWC
                     HPQMTVFGTLVFFLVMKFWMLILATTIPIPAGYFLPIFVYGAAIGRLFGEVLSLAFPE
                     GIVAGGKVSPIMPGAYALAGAAAFSGAVTHTLSTALLAFEVSGQIVHALPVLMAVLAA
                     NAICQSYQPSFYDGTIIVKKLPYLPWIRGRKIGSHLVTVGHFMNCTLTTLAKDMPLEE
                     VIKVVISTDVTQYPLVETTESQVLVGIVKRTHLVQSLHTDSASWAPGQQPCLQDILAN
                     GCPTQPVTLQLSTETSLHETHNLFELLNLQLLFVTSRGRAVGSVSWVELKKAISTLTN
                     PPAPK"
     exon            121..249
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            250..378
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            379..518
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            519..596
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            597..675
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            676..801
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            802..886
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            887..988
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1073
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1247
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1317
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1428
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1642
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1776
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1865
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1949
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2036
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2305
                     /gene="Clcnkb"
                     /gene_synonym="ClC-K2L; Clck2; Clcnk1l"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcacgctga cagagaaacc atggaggaaa tagtggggct tcgagagggc tcgcccagga
       61 agccagtgcc tctgcaagaa ctctggaggc cgtgcccacg aatccgcaga aacatccagg
      121 ggagcctaga gtggctgaaa gagcggctgt tccgtgtggg tgaggactgg tacttcctgg
      181 tggctctcgg ggtgctcatg gctctgatca gctatgccat gaactttgct attggacgtg
      241 tggtcagagc gcataagtgg ctgtacaggg agattgggga cggccacctg ctccggtacc
      301 tctcttggac cgtgtacccc gtggctcttc tgtccttctc ctccggcttc tcacagagca
      361 tcacaccatc ctctggaggg tctgggatcc ccgaggtgaa aaccatcttg acaggtgtga
      421 tcctggagga ctacctagac attaagaact tcggggccaa ggtggtgggc ctctcctgca
      481 ccctggcaac aggcagtacc atcttcctgg gaaaactggg cccctttgtg cacctgagcg
      541 tgatgatcgc tgcttacctg ggccgcgtgc gcaccaagac cgtcggggaa cctgagaaca
      601 agaccaaaga aatggaattg ttggctgcag gagcagcagt gggtgtggcc acggtcttcg
      661 ccgccccaat cagtggtgtt ctgttcagca tcgaggtcat gtcctctcac ttctccgtct
      721 gggattactg gaggggcttc ttcgctgcca cctgcggggc cttcatgttt cacctcctgg
      781 cggtcttcaa cagtgaacag gagaccatca cctccattta caagaccagc ttcccagtgg
      841 acataccctt tgacttacca gagatcttct tttttgtggc cttgggggcc atctgtggca
      901 tcctgagttg cgggtacaac tactgccagc ggacttcctt atttttcctc aagtccaatg
      961 ggttcacctc caaactgctg gctaccagca agcccttgta ctcagctctg gctgctgtgg
     1021 tcctggcctc catcacctat ccacctggtg tgggtcgctt catggcttcc cggctgtcta
     1081 tgtcagagta cttggagaca ctgtttgaca acaactcttg ggcactgatg accaagaact
     1141 cgtccccacc ctggtcagcg gagcctgatc cccagaacct gtggttggaa tggtgtcatc
     1201 cacagatgac tgtctttggg acactagtct tcttcctggt catgaagttc tggatgctga
     1261 ttttggccac caccatcccc atccccgcag gatacttttt gcccatcttt gtctacggag
     1321 ctgccatcgg gcgcctcttt ggggaggttc tatctttggc cttcccagag ggcattgtgg
     1381 ctggaggcaa ggtcagtccc atcatgcccg gggcctatgc tctggcaggt gctgctgcct
     1441 tctcaggggc ggtgacccac accctctcca cagcattgct ggcctttgag gtatccggcc
     1501 agatcgttca tgcactgcct gtgctgatgg ccgtgctggc ggccaatgcc atctgtcaga
     1561 gctaccagcc ctctttctac gacggcacca tcattgtcaa gaaactgccg tacctgccgt
     1621 ggatccgtgg tcgtaagatt ggttcccacc ttgtgactgt gggtcacttc atgaactgta
     1681 ccctcaccac gctggccaag gacatgcccc tggaggaagt gatcaaagtt gtgatctcca
     1741 cggatgtgac tcagtacccc ctggtggaga cgacagagtc tcaggtcctg gtgggcattg
     1801 tcaaaaggac ccacttggtg cagtccctcc ataccgattc agcttcctgg gctccaggcc
     1861 agcagccctg tctccaggat atcttggcta acggctgccc cacccagcca gtgacacttc
     1921 agctgtccac ggagacctcc ctgcatgaga cgcacaacct ctttgagctg ctgaaccttc
     1981 agctgctgtt cgtgacgtca cgaggcagag ctgtgggctc tgtgtcctgg gtggagctga
     2041 agaaagcaat ttcaaccctg accaatccgc ccgctcccaa gtgagtcagc ctgggagtcg
     2101 gcagcctggc gcccagtcgc tctcagctgc tgcagggcat ctccactttg cagaggagag
     2161 gactccacat tcacctctcc ccaccctgaa ggagctggtt gtaaatgaag acaccaatgc
     2221 gttctgtcac atcgggaccc ttggtccttc tagggttgtc tgctgctaac caagacttct
     2281 gagaaaaata aaactgattt ctggaaaaaa aaa
//
