LOCUS       NM_053327               2395 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Rattus norvegicus chloride voltage-gated channel Ka (Clcnka), mRNA.
ACCESSION   NM_053327 XM_001072716
VERSION     NM_053327.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Kim,Y.M., Kim,W.Y., Lee,H.W., Kim,J., Kwon,H.M., Klein,J.D.,
            Sands,J.M. and Kim,D.
  TITLE     Urea and NaCl regulate UT-A1 urea transporter in opposing
            directions via TonEBP pathway during osmotic diuresis
  JOURNAL   Am J Physiol Renal Physiol 296 (1), F67-F77 (2009)
   PUBMED   18945830
  REMARK    GeneRIF: Changes in CLC-K1, UT-A1, UT-A3, and TonEBP play important
            roles in the renal response to osmotic diuresis in an attempt to
            minimize changes in plasma osmolality and maintain water
            homeostasis.
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Markovic,S. and Dutzler,R.
  TITLE     The structure of the cytoplasmic domain of the chloride channel
            ClC-Ka reveals a conserved interaction interface
  JOURNAL   Structure 15 (6), 715-725 (2007)
   PUBMED   17562318
REFERENCE   3  (bases 1 to 2395)
  AUTHORS   Scholl,U., Hebeisen,S., Janssen,A.G., Muller-Newen,G., Alekov,A.
            and Fahlke,C.
  TITLE     Barttin modulates trafficking and function of ClC-K channels
  JOURNAL   Proc Natl Acad Sci U S A 103 (30), 11411-11416 (2006)
   PUBMED   16849430
REFERENCE   4  (bases 1 to 2395)
  AUTHORS   Schlingmann,K.P., Konrad,M., Jeck,N., Waldegger,P., Reinalter,S.C.,
            Holder,M., Seyberth,H.W. and Waldegger,S.
  TITLE     Salt wasting and deafness resulting from mutations in two chloride
            channels
  JOURNAL   N Engl J Med 350 (13), 1314-1319 (2004)
   PUBMED   15044642
REFERENCE   5  (bases 1 to 2395)
  AUTHORS   Wolf,K., Meier-Meitinger,M., Bergler,T., Castrop,H., Vitzthum,H.,
            Riegger,G.A., Kurtz,A. and Kramer,B.K.
  TITLE     Parallel down-regulation of chloride channel CLC-K1 and barttin
            mRNA in the thin ascending limb of the rat nephron by furosemide
  JOURNAL   Pflugers Arch 446 (6), 665-671 (2003)
   PUBMED   12759757
REFERENCE   6  (bases 1 to 2395)
  AUTHORS   Matsumura,Y., Uchida,S., Kondo,Y., Miyazaki,H., Ko,S.B., Hayama,A.,
            Morimoto,T., Liu,W., Arisawa,M., Sasaki,S. and Marumo,F.
  TITLE     Overt nephrogenic diabetes insipidus in mice lacking the CLC-K1
            chloride channel
  JOURNAL   Nat Genet 21 (1), 95-98 (1999)
   PUBMED   9916798
REFERENCE   7  (bases 1 to 2395)
  AUTHORS   Uchida,S., Sasaki,S., Nitta,K., Uchida,K., Horita,S., Nihei,H. and
            Marumo,F.
  TITLE     Localization and functional characterization of rat kidney-specific
            chloride channel, ClC-K1
  JOURNAL   J Clin Invest 95 (1), 104-113 (1995)
   PUBMED   7814604
REFERENCE   8  (bases 1 to 2395)
  AUTHORS   Uchida,S., Sasaki,S., Furukawa,T., Hiraoka,M., Imai,T., Hirata,Y.
            and Marumo,F.
  TITLE     Molecular cloning of a chloride channel that is regulated by
            dehydration and expressed predominantly in kidney medulla
  JOURNAL   J Biol Chem 269 (29), 19192 (1994)
   PUBMED   8034678
  REMARK    Correction to:[J Biol Chem. 1993 Feb 25;268(6):3821-4.. PMID:
            7680033]
REFERENCE   9  (bases 1 to 2395)
  AUTHORS   Kieferle,S., Fong,P., Bens,M., Vandewalle,A. and Jentsch,T.J.
  TITLE     Two highly homologous members of the ClC chloride channel family in
            both rat and human kidney
  JOURNAL   Proc Natl Acad Sci U S A 91 (15), 6943-6947 (1994)
   PUBMED   8041726
REFERENCE   10 (bases 1 to 2395)
  AUTHORS   Uchida,S., Sasaki,S., Furukawa,T., Hiraoka,M., Imai,T., Hirata,Y.
            and Marumo,F.
  TITLE     Molecular cloning of a chloride channel that is regulated by
            dehydration and expressed predominantly in kidney medulla
  JOURNAL   J Biol Chem 268 (6), 3821-3824 (1993)
   PUBMED   7680033
  REMARK    Erratum:[J Biol Chem. 1994 Jul 22;269(29):19192.. PMID: 8034678]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Nov 26, 2020 this sequence version replaced NM_053327.1.
            
            Summary: This gene is a member of the CLC family of voltage-gated
            chloride channels. The gene is located adjacent to a highly similar
            chloride channel gene on chromosome 5. This gene is syntenic with
            human CLCNKB (geneID:1188).[provided by RefSeq, Sep 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D13927.1, SRR26643287.14834.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                JAXUCZ010000005.1  158989110-158989148 c
            40-144              JAXUCZ010000005.1  158988412-158988516 c
            145-273             JAXUCZ010000005.1  158987696-158987824 c
            274-402             JAXUCZ010000005.1  158986984-158987112 c
            403-542             JAXUCZ010000005.1  158986085-158986224 c
            543-620             JAXUCZ010000005.1  158985480-158985557 c
            621-699             JAXUCZ010000005.1  158985319-158985397 c
            700-825             JAXUCZ010000005.1  158984439-158984564 c
            826-910             JAXUCZ010000005.1  158982943-158983027 c
            911-1012            JAXUCZ010000005.1  158982750-158982851 c
            1013-1097           JAXUCZ010000005.1  158982092-158982176 c
            1098-1271           JAXUCZ010000005.1  158981667-158981840 c
            1272-1341           JAXUCZ010000005.1  158980452-158980521 c
            1342-1452           JAXUCZ010000005.1  158980187-158980297 c
            1453-1666           JAXUCZ010000005.1  158979278-158979491 c
            1667-1800           JAXUCZ010000005.1  158978068-158978201 c
            1801-1889           JAXUCZ010000005.1  158976672-158976760 c
            1890-1973           JAXUCZ010000005.1  158976456-158976539 c
            1974-2060           JAXUCZ010000005.1  158974848-158974934 c
            2061-2395           JAXUCZ010000005.1  158974191-158974525 c
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..2395
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /note="chloride voltage-gated channel Ka"
                     /db_xref="GeneID:79425"
                     /db_xref="RGD:68435"
     exon            1..39
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            40..144
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..2108
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /note="chloride channel K1; clC-K1; chloride channel Ka;
                     chloride channel, voltage-sensitive Ka"
                     /codon_start=1
                     /product="chloride channel protein ClC-Ka"
                     /protein_id="NP_445779.2"
                     /db_xref="GeneID:79425"
                     /db_xref="RGD:68435"
                     /translation="MEELVGLREGSSGKPVTLQELWGPCPRIRRGVRRGLEWLKERLF
                     RVGEDWHFLVALGVLMALISYAMNFAIGRVVRAHKWLYREVGDGHLLRYLSWTVYPVA
                     LLSFSSGFSQSISPFSGGSGLPELKTMLSGVVLEDYLDIKNFGAKVVGLSCTLATGST
                     IFLGKVGPFVHLSVMISAYLGRVRAKTIGETENKAKEIEMLSAAAAVGVATVFAAPFS
                     GVLFSIEVMSSHFSVWNYWRGFFAATCGAFMFRLLGVFNSEQETITSIYKTRFRVDVP
                     FDLPEIFFFVALGFICGVLSCAYLFCQRTFLCFIKTNRYTSRLLATSKPSYAALVALV
                     LASITYPPGVGRFMASRLSMAEHLHSLFDNNSWALMTRNSSPPWPAEPDPQNLWLEWC
                     HPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFIIGAAIGRLLGEALSVAFPE
                     GIVAGREVNPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAA
                     NAISQNCQPSFYDGTIMAKKLPYLPWIRGRQIGSYPVTVEHFMNCNLTTLAKDTPLEE
                     VVKVVTSTEVSQYPLVETRESQTLVGIVERTHLVQALQTQPASWAPGQERFLQDILAG
                     GCPTQPVTLQLSPETSLYQAHSLFERLTLQSLFVTSRGKAVGSVSWAELKKAISTLIN
                     PPAPK"
     exon            145..273
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            274..402
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            403..542
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..620
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..699
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            700..825
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..910
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1012
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1097
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1271
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1341
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1452
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1666
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1800
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1889
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1973
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2060
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2395
                     /gene="Clcnka"
                     /gene_synonym="Clcnk1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgccagcca cgcaggacct ctgcagggtg gactgagagg acggatggaa gaactcgtgg
       61 gactgcgtga gggctcctct gggaagccag taactctgca agagctatgg gggccgtgtc
      121 cacgcatccg ccgaggtgtc agaaggggcc tagagtggct gaaagagcgg ttgttccgtg
      181 tgggtgagga ctggcacttc ctggtggctc tcggggtgct catggctctg atcagctatg
      241 ccatgaactt tgccattgga cgtgtggtca gagcacacaa atggctatac cgggaggttg
      301 gggacggcca cctgctccgg tacctctcct ggaccgtcta ccctgtggct cttctgtcct
      361 tctcctccgg cttctcgcag agcatctcac ccttctctgg agggtctgga ctcccagagc
      421 tgaagaccat gctgtccggt gtggtcctgg aggactacct agacatcaag aacttcgggg
      481 ccaaggtggt gggcctctcc tgcaccctgg caacaggcag taccatcttc ctaggcaaag
      541 tgggcccctt cgtgcacctg agcgtgatga tttctgctta tctgggccga gtgcgcgcca
      601 agaccattgg ggaaactgag aacaaggcca aggaaattga aatgctctcg gcggcagcgg
      661 cagtgggcgt ggccacagtt ttcgcagccc ccttcagcgg tgtcctgttc agcatcgagg
      721 tcatgtcctc tcacttctcc gtctggaatt actggagggg cttctttgcc gccacatgcg
      781 gggccttcat gttccgtctc ctaggggtct tcaacagtga acaggagacc atcacctcca
      841 tctacaagac cagattccga gtggatgtgc cctttgacct gcctgaaatc ttcttctttg
      901 tggctctggg gttcatctgc ggggtcctga gctgcgcgta cctgttctgt cagagaactt
      961 ttctctgctt tatcaagacc aatcggtaca cctccagact gttggctaca agcaagccgt
     1021 cctacgcagc tctggttgcc ctggtcctgg cctccatcac ctatccacct ggtgtgggtc
     1081 gcttcatggc ctcccggctg tccatggcag agcatttgca ctctctgttt gacaacaact
     1141 cttgggcact gatgacccga aactcatccc caccctggcc tgcggagccg gacccccaga
     1201 acctgtggtt ggaatggtgt cacccacggt tcaccatctt tgggactctg gccttcttcc
     1261 tggtcatgaa gttctggatg ctgattctgg ctaccacgat ccccatgcct gctgggtatt
     1321 tcatgcccat attcatcatt ggagctgcca ttgggcgcct cttgggagag gccctgtctg
     1381 tcgccttccc agagggcatt gtggctggca gagaggtcaa ccccatcatg cctgggggct
     1441 atgctctggc aggtgctgct gccttctcgg gggcggtgac ccacaccatc tccacagcac
     1501 tgctggcctt tgagctgacc ggccagatcg ttcatgcact gcctgtgctg atggccgtgc
     1561 tggcggccaa tgccatctct caaaactgtc agccgtcttt ctacgatggc accatcatgg
     1621 ccaagaaact gccgtacctg ccgtggatcc gtggccgaca gattggctcc taccctgtga
     1681 ctgtggaaca cttcatgaac tgtaacctca ccacgctggc caaggacacg cccctggagg
     1741 aggtggtcaa agttgtgacc tctacagaag tgtctcaata tcccttggtg gagaccagag
     1801 agtctcagac cctggtgggc attgtggaac ggacccactt ggtgcaagcc ctccagaccc
     1861 agccagcttc ctgggctcca ggccaagagc gctttctcca ggacatcttg gcaggtggct
     1921 gccccacaca gccagtgacc ctgcagctgt ccccagagac ctccctgtat caggcacaca
     1981 gcctctttga gcggctgacc cttcagtcgc tgttcgtgac gtcacggggc aaagctgtgg
     2041 gctctgtgtc ctgggcggag ctgaagaaag ccatttccac cttgatcaac ccaccagccc
     2101 ccaagtgagc cagcgttggg gtcagaacag gaccccagct aagagctgca gaggctgcac
     2161 agagccccac cccatttccc tccctacccc acccctccag cccagcccgg ctccccagtg
     2221 cctcaggcag ctctacccag ctgaacggga ctccgtattc atcatggcct acaccgagag
     2281 cacaacatct ctctggtctg gaagggacaa atctcaccct ggtgcatgtc ccttctggga
     2341 ttatctattc acaaccagta gctcctgaga aaataaaagt aaagctggtt cccag
//
