LOCUS       NM_017137               3153 bp    mRNA    linear   ROD 05-AUG-2024
DEFINITION  Rattus norvegicus chloride voltage-gated channel 2 (Clcn2), mRNA.
ACCESSION   NM_017137
VERSION     NM_017137.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Liu,Y., Yang,H., Zeng,R., He,L., Xiao,T., Peng,X., Kuang,Z. and
            Wu,L.
  TITLE     NEDD4-2 and the CLC-2 channel regulate neuronal excitability in the
            pathogenesis of mesial temporal lobe epilepsy
  JOURNAL   Sci Rep 14 (1), 4835 (2024)
   PUBMED   38418461
  REMARK    GeneRIF: NEDD4-2 and the CLC-2 channel regulate neuronal
            excitability in the pathogenesis of mesial temporal lobe epilepsy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Scholl,U.I., Stolting,G., Schewe,J., Thiel,A., Tan,H.,
            Nelson-Williams,C., Vichot,A.A., Jin,S.C., Loring,E., Untiet,V.,
            Yoo,T., Choi,J., Xu,S., Wu,A., Kirchner,M., Mertins,P., Rump,L.C.,
            Onder,A.M., Gamble,C., McKenney,D., Lash,R.W., Jones,D.P.,
            Chune,G., Gagliardi,P., Choi,M., Gordon,R., Stowasser,M., Fahlke,C.
            and Lifton,R.P.
  TITLE     CLCN2 chloride channel mutations in familial hyperaldosteronism
            type II
  JOURNAL   Nat Genet 50 (3), 349-354 (2018)
   PUBMED   29403011
REFERENCE   3  (bases 1 to 3153)
  AUTHORS   Hou,X., Zhang,R., Wang,J., Li,Y., Li,F., Zhang,Y., Zheng,X.,
            Shen,Y., Wang,Y. and Zhou,L.
  TITLE     CLC-2 is a positive modulator of oligodendrocyte precursor cell
            differentiation and myelination
  JOURNAL   Mol Med Rep 17 (3), 4515-4523 (2018)
   PUBMED   29344669
REFERENCE   4  (bases 1 to 3153)
  AUTHORS   He,F., Peng,Y., Yang,Z., Ge,Z., Tian,Y., Ma,T. and Li,H.
  TITLE     Activated ClC-2 Inhibits p-Akt to Repress Myelination in GDM
            Newborn Rats
  JOURNAL   Int J Biol Sci 13 (2), 179-188 (2017)
   PUBMED   28255270
  REMARK    GeneRIF: ClC-2 and caspase-3 were induced by GDM, which resulted in
            apoptosis and myelination inhibition. The effect was caused by
            repressing PI3K-Akt signaling pathway. Application of ClC-2
            inhibitor DIDS showed protective effects on cerebral white matter
            damage stimulated by high glucose concentration.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3153)
  AUTHORS   Jeworutzki,E., Lopez-Hernandez,T., Capdevila-Nortes,X., Sirisi,S.,
            Bengtsson,L., Montolio,M., Zifarelli,G., Arnedo,T., Muller,C.S.,
            Schulte,U., Nunes,V., Martinez,A., Jentsch,T.J., Gasull,X.,
            Pusch,M. and Estevez,R.
  TITLE     GlialCAM, a protein defective in a leukodystrophy, serves as a
            ClC-2 Cl(-) channel auxiliary subunit
  JOURNAL   Neuron 73 (5), 951-961 (2012)
   PUBMED   22405205
REFERENCE   6  (bases 1 to 3153)
  AUTHORS   Chu,S. and Zeitlin,P.L.
  TITLE     Alternative mRNA splice variants of the rat ClC-2 chloride channel
            gene are expressed in lung: genomic sequence and organization of
            ClC-2
  JOURNAL   Nucleic Acids Res 25 (20), 4153-4159 (1997)
   PUBMED   9321672
REFERENCE   7  (bases 1 to 3153)
  AUTHORS   Chu,S., Murray,C.B., Liu,M.M. and Zeitlin,P.L.
  TITLE     A short CIC-2 mRNA transcript is produced by exon skipping
  JOURNAL   Nucleic Acids Res 24 (17), 3453-3457 (1996)
   PUBMED   8811102
REFERENCE   8  (bases 1 to 3153)
  AUTHORS   Staley,K., Smith,R., Schaack,J., Wilcox,C. and Jentsch,T.J.
  TITLE     Alteration of GABAA receptor function following gene transfer of
            the CLC-2 chloride channel
  JOURNAL   Neuron 17 (3), 543-551 (1996)
   PUBMED   8816717
REFERENCE   9  (bases 1 to 3153)
  AUTHORS   Grunder,S., Thiemann,A., Pusch,M. and Jentsch,T.J.
  TITLE     Regions involved in the opening of CIC-2 chloride channel by
            voltage and cell volume
  JOURNAL   Nature 360 (6406), 759-762 (1992)
   PUBMED   1334533
REFERENCE   10 (bases 1 to 3153)
  AUTHORS   Thiemann,A., Grunder,S., Pusch,M. and Jentsch,T.J.
  TITLE     A chloride channel widely expressed in epithelial and
            non-epithelial cells
  JOURNAL   Nature 356 (6364), 57-60 (1992)
   PUBMED   1311421
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            X64139.1.
            
            On Nov 20, 2020 this sequence version replaced NM_017137.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X64139.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3153              X64139.1           52-3204
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q23"
     gene            1..3153
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /note="chloride voltage-gated channel 2"
                     /db_xref="GeneID:29232"
                     /db_xref="RGD:2361"
     exon            1..198
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..2841
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /note="chloride channel 2; chloride channel,
                     voltage-sensitive 2"
                     /codon_start=1
                     /product="chloride channel protein 2"
                     /protein_id="NP_058833.1"
                     /db_xref="GeneID:29232"
                     /db_xref="RGD:2361"
                     /translation="MAAATAAAATVAGEGMEPRALQYEQTLMYGRYTQELGAFAKEEA
                     ARIRLGGPEPWKGSPSARATPELLEYGQSRCARCRICSVRCHKFLVSRVGEDWIFLVL
                     LGLLMALVSWAMDYAIAVCLQAQQWMSRGLNTNILLQYLAWVTYPVVLITFSAGFTQI
                     LAPQAVGSGIPEMKTILRGVVLKEYLTLKTFVAKVIGLTCALGSGMPLGKEGPFVHIA
                     SMCAALLSKFLSLFGGIYENESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFF
                     AVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIG
                     IASGFGGALFVYLNRKIVQVMRKQKTINRFLMKKRLLFPALVTLLISTLTFPPGFGQF
                     MAGQLSQKETLVTLFDNRTWVRQGLVEDLGAPSTSQAWSPPRANVFLTLVIFILMKFW
                     MSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVV
                     GAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIK
                     KLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESP
                     ESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQMSPPSDQESPPSSETSIRFQ
                     VNTEDSGFPGAHGQTHKPLKPALKRGPSNATSLQEGTTGNMESAGIALRSLFCGSPPL
                     ESTTSELEKSESCDKRKLKRVRISLASDSDLEGKMSPEEILEWEEQQLDEPVNFSDCK
                     IDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQG
                     VKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKCQ"
     exon            199..355
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            356..487
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            488..616
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            617..750
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            751..828
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            829..907
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1033
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1118
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1220
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1305
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1461
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1531
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1642
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1856
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..1990
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2163
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2278
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2355
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2356..2415
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2454
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2559
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2560..2646
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..3153
                     /gene="Clcn2"
                     /gene_synonym="ClC-2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 actcggccgc ccgggctaag gggacaggac gggtgcgggc gcgggctcag cgagccggga
       61 gctgagtcaa ggccagagcc cgagaagcaa gaggaggcaa gaggacagtg caccgagatg
      121 gcggccgcaa cggccgcggc ggcgacggtg gcgggggaag ggatggagcc tcgagcgctg
      181 cagtacgagc agaccctgat gtatggccgg tatactcagg aactcggggc ctttgccaaa
      241 gaggaagctg ctcggattcg cctgggaggg cctgagccct ggaaggggtc cccttctgcc
      301 cgggctaccc cagagctcct agaatatgga cagagccgat gtgccagatg tcgcatttgt
      361 tctgtacgct gccacaagtt cctagtgtcc agggtcggtg aagattggat cttcctggtc
      421 ctgttggggc tcctcatggc attggtcagt tgggccatgg actatgccat cgctgtctgt
      481 ctacaggctc agcaatggat gtcccggggc ttaaacacca acatcttact ccagtacctg
      541 gcttgggtta cctaccctgt cgtcctcatc actttctctg ccggattcac ccagatcctg
      601 gccccgcagg ctgttgggtc tggcatcccc gagatgaaaa ccatccttcg gggagtggtg
      661 ctgaaagagt acctcaccct caagaccttt gtagctaagg tcatcgggct gacctgtgcc
      721 ctgggcagtg ggatgcccct tggcaaggag ggaccctttg tgcacattgc cagcatgtgt
      781 gctgcccttc tcagcaagtt cctctccctc tttgggggta tctatgagaa cgagtcccgg
      841 aacacagaga tgctagctgc cgcatgcgca gtaggagtgg gctgctgctt tgccgcacca
      901 atcggagggg tcctattcag cattgaggtc acctccacct tcttcgctgt taggaactac
      961 tggcggggct tctttgcggc caccttcagt gccttcatct ttcgggtctt ggcagtgtgg
     1021 aaccgtgatg aagaaaccat cacagctctc ttcaaaactc ggttccgact cgacttcccc
     1081 tttgacctgc aggagctgcc agcttttgct gtcattggta ttgctagtgg cttcggggga
     1141 gccctctttg tctacctgaa ccggaagatt gtccaggtga tgcggaagca aaaaaccatc
     1201 aaccgcttcc tcatgaagaa acggctgctc ttccccgcac tggtgactct gctcatctcc
     1261 actctgacct tcccccctgg ctttggacag ttcatggccg gacagctctc acagaaagag
     1321 accctggtca cactgtttga caaccggaca tgggtccgcc agggcctggt agaggatcta
     1381 ggagcaccta gcacttcaca ggcctggagc ccaccgcgcg ccaacgtctt cctgactctc
     1441 gtcatcttca tcctcatgaa gttctggatg tctgcactgg ccaccaccat cccagtgccc
     1501 tgtggtgcct tcatgcctgt ctttgtcatt ggagcagcat ttggccggct ggtgggcgag
     1561 agcatggctg cctggttccc agatgggatt cacacggata gcagtaccta ccggattgta
     1621 cctggaggct atgccgtggt cggggcagct gcacttgcag gagcagtgac acacacagtg
     1681 tccacagcag tgattgtctt cgagctcacc ggccagattg cccacattct gcccgtcatg
     1741 attgctgtca tcctggcgaa tgccgttgcc cagagcctgc agccctccct ctatgacagt
     1801 atcatccgca tcaagaagct gccctacctg cctgagctgg gctggggccg ccaccagcag
     1861 taccgggtac gagtagagga catcatggta cgggatgtac cccatgtagc cctcagctgc
     1921 acttttcggg acctgcgact ggcactgcac cgaaccaagg gccgtatgtt ggccctagtg
     1981 gagtcacctg agtccatgat tctgctgggc tccatcgaac gctcacaggt ggtagcgcta
     2041 ctgggagcac agctgagtcc agcgcgccgg cggcagcaca tgcaaaagct aaggaaagcc
     2101 cagatgtccc caccgtcaga tcaggagagc ccccccagct ctgagacgtc catccgcttc
     2161 caggtgaaca cagaggactc aggcttccct ggagcccatg ggcagactca caagcccctg
     2221 aagcctgctc taaagagagg gcccagcaac gccacaagcc tccaggaagg taccacgggc
     2281 aacatggaat cagcaggcat tgccctcaga agcctcttct gtggcagtcc acctctggag
     2341 tcgacgacgt cagaattgga aaagtcggaa tcctgtgaca aacgcaagct gaagcgggtc
     2401 cgaatctccc tggcgagtga ctcagacctg gaaggcaaga tgagtcccga ggagatctta
     2461 gagtgggaag agcagcagct agatgagcca gtcaacttca gtgactgcaa aatcgacccc
     2521 gcccccttcc agctagtgga gcggacttct ttgcacaaga cccacaccat cttctcgctg
     2581 ctgggagtgg accatgctta tgtcaccagc attggcagac tcattgggat tgtcacccta
     2641 aaggagctcc ggaaggccat tgaaggctct gtcacagcac agggtgtaaa agtcaggcca
     2701 ccccttgcca gtttccggga cagtgccacc agcagcagtg acacagagac caccgaggtg
     2761 catgcactct gggggccgag atcccgccac ggcctcccac gagagggtac cccctctgat
     2821 agtgatgaca agtgccagtg acccccatac tggacggcct gtcttccttc tgcaagccag
     2881 cagtgaccag aagctggaac ctgaggccat cctcactgtg ggctgccagc tcccccattt
     2941 tcaacacctc ccagccggag gaccagcaat gctctggcag gaggtggggt tctgtggccc
     3001 ctgcctcgtc ttttgagaaa agggcagaac taaaatgggt ttatactgga acctccaatg
     3061 acaagatgta tatagaaatt tacaaagatt tttatattaa tttaataaaa caataaatag
     3121 aataaacacc taattagcca ctcatgtata gaa
//
