LOCUS       NM_001077356            2899 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus chloride channel calcium activated 4-like
            (Clca4l), mRNA.
ACCESSION   NM_001077356 XM_001063517 XM_001079188
VERSION     NM_001077356.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Mura,C.V., Delgado,R., Delgado,M.G., Restrepo,D. and Bacigalupo,J.
  TITLE     A CLCA regulatory protein present in the chemosensory cilia of
            olfactory sensory neurons induces a Ca2+-activated Cl- current when
            transfected into HEK293
  JOURNAL   BMC Neurosci 18 (1), 61 (2017)
   PUBMED   28800723
  REMARK    GeneRIF: Cloned and expressed CLCA4L from rat olfactory epithelium
            in HEK293 cells. In the transfected HEK293 cells we measured a
            Cl(-)-selective Ca(2+)-activated current, blocked by niflumic acid,
            not present in the non-transfected cells. CLCA4L mimics the CLCA1
            current on its ability to induce the ANO1-dependent
            Ca(2+)-activated Cl(-) current endogenous to these cells. A CLCA
            protein was detected in olfactory receptor cell cilia
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Yoon,I.S., Jeong,S.M., Lee,S.N., Lee,J.H., Kim,J.H., Pyo,M.K.,
            Lee,J.H., Lee,B.H., Choi,S.H., Rhim,H., Choe,H. and Nah,S.Y.
  TITLE     Cloning and heterologous expression of a Ca2+-activated chloride
            channel isoform from rat brain
  JOURNAL   Biol Pharm Bull 29 (11), 2168-2173 (2006)
   PUBMED   17077509
  REMARK    GeneRIF: Using rbCLCA1 sequence, gene rbCLCA2 was cloned containing
            sites for signal peptide cleavage, N-linked glycosylation and
            phosphorylation by PKC, cAMP- and cGMP-dependent protein
            kinases,tyrosine kinase and casein II. rbCLCA2 current is Ca(2+)
            dependent.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB256513.1.
            
            On Aug 30, 2012 this sequence version replaced NM_001077356.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB256513.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132283,
                                           SAMEA5760383 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2899              AB256513.1         2-2900
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q44"
     gene            1..2899
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /note="chloride channel calcium activated 4-like"
                     /db_xref="GeneID:499721"
                     /db_xref="RGD:1591945"
     exon            1..178
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..2737
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /note="calcium-activated chloride channel"
                     /codon_start=1
                     /product="chloride channel calcium activated 4-like
                     precursor"
                     /protein_id="NP_001070824.1"
                     /db_xref="GeneID:499721"
                     /db_xref="RGD:1591945"
                     /translation="MVPVLKVLLFLTLHLLQDVKSSKVHLNNNGYEGVVIAINPSVPE
                     DERLIPSLKEMVTQASTYLFEATRRRFYFRNVSILVPITWKSKTEYLTPKQESYDQAD
                     VIVADPHLKYGDDPYTLQYGQCGDRGQYIHFTPNFLLIDNLIIYGPRGRVFVHEWAHL
                     RWGVFDEYNKERPFYLSRKNVVEATRCSTDITGTNVVHECQGGSCVTRKCRRDSKTGL
                     PEPKCTFIPNKSQTARASIMFLQSLDSVVEFCTEKTHNTEAPNLQNKICNGRSTWDVI
                     KESADFQHAPPMRGTEAPPPPTFSLLKSRQRVVCLVLDKSGSMSSGDPITRLTLMNQA
                     AELYLIQILEKESLVGLVTFDSIAIVQNNLIRMINDSSYLEISAKLPQEAAGGTSICN
                     GLRKGFETITSSDQSTCGSEIVLLTDGEDDQISSCFEEVKHSGAVIHTIALGPDAARE
                     LETLSDMTGGRRFYASEGINGLIDAFSGISSKSGSLSQQALQLESKALEIKEKEWLSG
                     TVPVDSTVGNDTFFVVTWTVQKPEIILQDPKGKNYRTSDFQEDKLNIFSVRLQIPGIA
                     ETGTWTYSLFNKGVKPQLLTVTVTTRARSPTTQPLMATAHMSQSAAQYPSPMIVYARV
                     SQGFLPVLGANVTAVIESETGHQVTLVLWDNGAGADTLRNDGIYSRYFTEFHGNGRYS
                     LKVSAQARKNITKLNLKQKNKALYIPGYVENDQIILNPPRPEVPEEATEAAVEDFSRL
                     TSGESFTVSGAPPDGGHAHVFPPSKVTDLEAEFKGDHIHLTWTAPGKVLDKGRAHRYV
                     IRMSRHPPGLQDFNDATLVNTSGLIPKEAGSEETFKFKPASFKIENGTQIYIAIQAEN
                     EAGLTSEVSNIAQAVNFIPPREPSNPNLGNKMPVPCLTVLVFVATLFIF"
     exon            179..319
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            320..467
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            468..576
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            577..754
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            755..982
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1216
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1394
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1495
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1720
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1991
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2159
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2399
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
     exon            2400..2885
                     /gene="Clca4l"
                     /gene_synonym="Rbclca2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caccatagca tgtgcgagca tggtgccagt gctgaaggtc cttctgttcc tcaccctgca
       61 tctcttgcag gatgtgaaaa gctccaaggt ccatctcaac aacaatggat acgagggtgt
      121 ggtcattgca attaacccca gtgtaccaga ggatgaaaga ctcatcccaa gcttaaagga
      181 aatggtaact caagcctcca cttacctgtt tgaagccacc aggagaagat tttacttcag
      241 gaacgtgagc attctagttc ccataacctg gaagtcaaaa actgagtact taacaccgaa
      301 acaagaatca tatgaccaag cagacgtcat agttgccgat cctcatctga aatatggaga
      361 cgatccctac acccttcaat atgggcagtg tggggacaga ggacagtaca tacatttcac
      421 tccaaacttc ctgctcattg ataacttgat tatctatgga ccccgaggca gagtctttgt
      481 ccatgagtgg gcccatctcc ggtggggagt atttgatgag tacaacaaag aacgtccctt
      541 ctacctatcg agaaagaatg ttgttgaagc aacaaggtgt tctactgaca tcactggcac
      601 caatgtggtc catgagtgtc agggaggcag ttgcgtgaca agaaagtgcc ggcgagattc
      661 aaagacagga ctaccggaac ccaagtgtac atttatccca aacaaatccc agacagccag
      721 ggcctccata atgttcctgc aaagtctgga ttctgtggtt gaattttgca cagaaaaaac
      781 ccacaatacg gaagccccaa acctacaaaa caaaatatgc aatggcagaa gcacgtggga
      841 cgtaatcaag gagtccgctg actttcagca tgcccctccc atgagaggaa cagaagcccc
      901 tcctccaccc acgttttcct tgctcaagtc cagacagcga gtggtctgct tggtgctcga
      961 taaatccgga agcatgagct caggagaccc catcactcgt ctcaccctaa tgaaccaagc
     1021 agcagagctg tacttaattc aaatccttga gaaggaatcc ttggtcggcc tggtcacgtt
     1081 tgatagcatt gctatagtcc aaaataacct cataagaatg atcaacgaca gctcctacct
     1141 agagatcagt gcaaagcttc cccaagaagc tgctggcgga acttcaattt gcaacggact
     1201 cagaaaagga tttgagacaa ttacctccag tgaccagagt acttgcggtt ctgagattgt
     1261 attgctaaca gatggggaag atgatcaaat aagttcatgc tttgaggagg tcaaacacag
     1321 cggtgccgtc attcacacca ttgccctggg gcctgacgct gcccgagaac tggagactct
     1381 gtcggacatg acaggagggc gtcgctttta tgccagtgaa ggcatcaacg gcctgatcga
     1441 cgctttcagt gggatttcat ccaaaagtgg cagcctgtcc cagcaggctc tgcagttgga
     1501 gagcaaagcc ctggagatta aggagaagga atggctaagt ggcacagttc ccgtggacag
     1561 taccgtcggc aacgacacgt tctttgttgt cacctggaca gtacaaaagc cagaaattat
     1621 ccttcaagat ccgaaaggga aaaactacag gacctcggat ttccaggaag ataagctaaa
     1681 tatcttctct gttcgccttc agatcccagg catcgccgag acaggaactt ggacttacag
     1741 cctcttcaat aagggggtca aacctcagtt gctaactgtg acagtgacca ctcgagccag
     1801 aagtcccacc acgcagcccc tcatggccac cgctcacatg agtcagagcg cagcccagta
     1861 ccctagcccg atgattgtgt atgcacgggt cagccaaggg tttttgcctg ttctgggagc
     1921 caatgtcaca gctgtcatag aatctgaaac tggacatcaa gtcaccctgg tgctctggga
     1981 caacggggca ggtgcggata cactcagaaa tgatggcatc tactcaagat atttcacgga
     2041 gtttcatgga aacggcagat acagcttaaa agtcagtgcc caggcaagaa aaaacatcac
     2101 gaaactaaat ctgaaacaaa agaataaggc tctttatata cctggctacg ttgaaaatga
     2161 tcaaattata ctgaatccac ccagaccgga agtcccagaa gaagctacag aagctgcagt
     2221 ggaagacttc agcagactaa cctctggaga gtcgtttact gtgtccggag cgccccctga
     2281 tggcggccac gctcatgtgt tcccacccag taaagttaca gacctggagg ccgagtttaa
     2341 aggtgatcat attcacctta cctggacggc ccctggcaag gttcttgata aaggaagagc
     2401 gcacagatac gtcatcagaa tgagccggca tcctcctggt ctccaagatt ttaacgacgc
     2461 tactttagtg aatacttccg gtctgatacc taaagaggct ggctcagaag aaacgtttaa
     2521 attcaaacca gcaagtttta aaatagaaaa cggcacccag atctacattg caattcaggc
     2581 cgaaaatgaa gccggtctca cctctgaggt ctccaacatc gcacaggctg tcaactttat
     2641 tcctccacgg gaacccagca accctaatct gggtaacaaa atgcctgtac cctgtttgac
     2701 agttttggta tttgttgcaa ctttattcat attttaaact agaaatttgc agtagagcta
     2761 aaattccatc tttgaattga aatttactct cctcgttgtc tataactctt tataacataa
     2821 aagggaatgt tcaatactgt caatttccta tctgagtcta ttaaaattat aagttgctat
     2881 atgtcaaaaa aaaaaaaaa
//
