LOCUS       NM_053529               5295 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Rattus norvegicus class II, major histocompatibility complex,
            transactivator (Ciita), transcript variant 1, mRNA.
ACCESSION   NM_053529
VERSION     NM_053529.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Fredlund,F., Jimenez-Ferrer,I., Grabert,K., Belfiori,L.F., Luk,K.
            and Swanberg,M.
  TITLE     Ciita Regulates Local and Systemic Immune Responses in a Combined
            rAAV-alpha-synuclein and Preformed Fibril-Induced Rat Model for
            Parkinson's Disease
  JOURNAL   J Parkinsons Dis 14 (4), 693-711 (2024)
   PUBMED   38728204
  REMARK    GeneRIF: Ciita Regulates Local and Systemic Immune Responses in a
            Combined rAAV-alpha-synuclein and Preformed Fibril-Induced Rat
            Model for Parkinson's Disease.
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Jensen,V.F., Swanberg,M., Herlin,M., McGuigan,F.E., Jorgensen,N.R.
            and Akesson,K.E.
  TITLE     Differential expression of the inflammatory ciita gene may be
            accompanied by altered bone properties in intact sex
            steroid-deficient female rats
  JOURNAL   BMC Res Notes 16 (1), 372 (2023)
   PUBMED   38115045
  REMARK    GeneRIF: Differential expression of the inflammatory ciita gene may
            be accompanied by altered bone properties in intact sex
            steroid-deficient female rats.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5295)
  AUTHORS   Jimenez-Ferrer,I., Backstrom,F., Duenas-Rey,A., Jewett,M.,
            Boza-Serrano,A., Luk,K.C., Deierborg,T. and Swanberg,M.
  TITLE     The MHC class II transactivator modulates seeded alpha-synuclein
            pathology and dopaminergic neurodegeneration in an in vivo rat
            model of Parkinson's disease
  JOURNAL   Brain Behav Immun 91, 369-382 (2021)
   PUBMED   33223048
  REMARK    GeneRIF: The MHC class II transactivator modulates seeded
            alpha-synuclein pathology and dopaminergic neurodegeneration in an
            in vivo rat model of Parkinson's disease.
REFERENCE   4  (bases 1 to 5295)
  AUTHORS   Jimenez-Ferrer,I., Jewett,M., Tontanahal,A., Romero-Ramos,M. and
            Swanberg,M.
  TITLE     Allelic difference in Mhc2ta confers altered microglial activation
            and susceptibility to alpha-synuclein-induced dopaminergic
            neurodegeneration
  JOURNAL   Neurobiol Dis 106, 279-290 (2017)
   PUBMED   28736195
  REMARK    GeneRIF: Study found that natural allelic differences in the Mhc2ta
            gene regulating MHCII expression levels impact microglial
            phenotype, antigen presentation capacity and dopaminergic pathology
            after overexpression of human alpha-syn. These results indicate
            that Mhc2ta is a key upstream regulator of the inflammatory
            response in Parkinson's Disease pathology.
REFERENCE   5  (bases 1 to 5295)
  AUTHORS   Yang,Y., Dai,W., Chen,Z., Yan,Z., Yao,Z. and Zhang,C.
  TITLE     Downregulating immunogenicity of Schwann cells via inhibiting a
            potential target of class II transactivator (CIITA) gene
  JOURNAL   Biosci Trends 7 (1), 50-55 (2013)
   PUBMED   23524893
REFERENCE   6  (bases 1 to 5295)
  AUTHORS   Suzuki,K., Mori,A., Ishii,K.J., Saito,J., Singer,D.S.,
            Klinman,D.M., Krause,P.R. and Kohn,L.D.
  TITLE     Activation of target-tissue immune-recognition molecules by
            double-stranded polynucleotides
  JOURNAL   Proc Natl Acad Sci U S A 96 (5), 2285-2290 (1999)
   PUBMED   10051633
REFERENCE   7  (bases 1 to 5295)
  AUTHORS   Sims,T.N., Goes,N.B., Ramassar,V., Urmson,J. and Halloran,P.F.
  TITLE     In vivo class II transactivator expression in mice is induced by a
            non-interferon-gamma mechanism in response to local injury
  JOURNAL   Transplantation 64 (12), 1657-1664 (1997)
   PUBMED   9422398
REFERENCE   8  (bases 1 to 5295)
  AUTHORS   Fontes,J.D., Jiang,B. and Peterlin,B.M.
  TITLE     The class II trans-activator CIITA interacts with the
            TBP-associated factor TAFII32
  JOURNAL   Nucleic Acids Res 25 (12), 2522-2528 (1997)
   PUBMED   9171108
REFERENCE   9  (bases 1 to 5295)
  AUTHORS   Bontron,S., Steimle,V., Ucla,C., Eibl,M.M. and Mach,B.
  TITLE     Two novel mutations in the MHC class II transactivator CIITA in a
            second patient from MHC class II deficiency complementation group A
  JOURNAL   Hum Genet 99 (4), 541-546 (1997)
   PUBMED   9099848
REFERENCE   10 (bases 1 to 5295)
  AUTHORS   Mann,D.M. and Yates,P.O.
  TITLE     A quantitative study of the glia of the Purkinje cell layer of the
            cerebellum in mammals
  JOURNAL   Neuropathol Appl Neurobiol 5 (1), 71-76 (1979)
   PUBMED   107465
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Aug 2, 2012 this sequence version replaced NM_053529.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF251305.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-365               JAXUCZ010000010.1  5693978-5694342     c
            366-512             JAXUCZ010000010.1  5673693-5673839     c
            513-608             JAXUCZ010000010.1  5673387-5673482     c
            609-671             JAXUCZ010000010.1  5671299-5671361     c
            672-752             JAXUCZ010000010.1  5671085-5671165     c
            753-797             JAXUCZ010000010.1  5668756-5668800     c
            798-932             JAXUCZ010000010.1  5668166-5668300     c
            933-1097            JAXUCZ010000010.1  5666417-5666581     c
            1098-1166           JAXUCZ010000010.1  5665737-5665805     c
            1167-2808           JAXUCZ010000010.1  5662820-5664461     c
            2809-2967           JAXUCZ010000010.1  5661870-5662028     c
            2968-3039           JAXUCZ010000010.1  5660719-5660790     c
            3040-3120           JAXUCZ010000010.1  5657067-5657147     c
            3121-3213           JAXUCZ010000010.1  5656281-5656373     c
            3214-3300           JAXUCZ010000010.1  5653635-5653721     c
            3301-3384           JAXUCZ010000010.1  5650785-5650868     c
            3385-3468           JAXUCZ010000010.1  5650590-5650673     c
            3469-3565           JAXUCZ010000010.1  5649330-5649426     c
            3566-5295           JAXUCZ010000010.1  5647085-5648814     c
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q11"
     gene            1..5295
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /note="class II, major histocompatibility complex,
                     transactivator"
                     /db_xref="GeneID:85483"
                     /db_xref="RGD:619813"
     exon            1..365
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..3544
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; MHC
                     class II transactivator type IV"
                     /codon_start=1
                     /product="MHC class II transactivator isoform 1"
                     /protein_id="NP_445981.2"
                     /db_xref="GeneID:85483"
                     /db_xref="RGD:619813"
                     /translation="MNHFQAILAQVQTLLSSQQPRQVRALLDGLLEEELLSREYHCAL
                     LHEPDGEALARKISLTLLEKGDLDLAFVSWVCNRLQVPEVERGPNCRDHGGHTLCATM
                     ELGSPEGSYLELLNSDADPLHLYHLYDQMDLAGEEEIELNSETDMDTINCDQFSKLLQ
                     DMEMDEETREAYANIAELDQYVFQDTQLEGLSKDLFIQHIGPEEGFGENVEGPAESGE
                     KPQKRRFPEEHAMDSKHRKLVPPSRTSLNYLDLPAGHIQIFTTLPQGLWQISGVGTGL
                     SSVLVYHGEMPQVNQLLSSSSPTVPSLPESPDRPGSTSPFAPSTADLPGMPEPALTSR
                     VNETEDTTPPSPCQEDPEPSIKLPKWPESVERFQHSLQDKYQALPGSPSGPLVAMELV
                     RARLERGSNKSQERELATPDWTERQLAHGGLAEVLRVVGDSRRSRETQVVAVLGKAGQ
                     GKSHWARTVSHIWAQGQLPQYDFVFYVPCQCLDRPGDTYHLRDLLCPPNLQPLAMDDE
                     VIDHIVRQPDRVLLILDAFEELEAQDSLLHGPCGPLSPEPCSLRGLLAGLFQRKLLRG
                     CTLLLTARPRGRLAQSLSKADAIFEVPSFSTKQAQIYMRHYFENSGTAGSHDKALGLL
                     EGQPFLLTHSHSPVLCSAVCQLSKALLDQGAEAQLPCTLTGLYVGLLGPAAQNSPPGA
                     LVELAKLAWELGRRHQSTLQETQFPSVEVKTWAVAQGLMQQTTEAQLAFSSFLLQCFL
                     GAVWLAQCNEMKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLVFQPRAHCLGALVE
                     PAMAAVADRKRKVLTRYLKRLKLGTLRAGRLLELLHCAHETQEPGIWKHVSHQLPGHL
                     SFLGTRLTAPDVYVLGRALETASQDFSLDLRQTGIEPSRLGDLVGLSCVTSFRASLSD
                     TMALWESLQHQGETQLLQAAEEKFTIEPFKAKSPKDVEDLDSLVQTQRLRNPSADAAK
                     DLPAIRDLKKLEFALGPVLGPQAFPTLAKILPAFSSLQHLDLDSLSENKIGDKGVSKL
                     SATFPQLKALETLNLSQNSITDVGACKLAEALPALAKSLLRLSLYNNCICDEGAKSLA
                     RVLPDMVSLRVMDVQFNKFTAVGAQQLTSSLQKCPQVETLAMWTPTIPFGVQEHLQQL
                     DARISLR"
     exon            366..512
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            513..608
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            609..671
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            672..752
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            753..797
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            798..932
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1097
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1166
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..2808
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            2809..2967
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            2968..3039
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            3040..3120
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            3121..3213
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            3214..3300
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            3301..3384
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            3385..3468
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3565
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
     exon            3566..5295
                     /gene="Ciita"
                     /gene_synonym="C2ta; Mhc2ta"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcagaggttc ctgggtctca cttcatgttt tggattctgc gagactggat gagaggcggc
       61 tggcctgcct cagagcactg ccatgaacca cttccaggcc atcctggccc aggtgcagac
      121 actgctgtcc agtcagcagc ccaggcaggt gcgggccctc ctggatggct tgctggaaga
      181 agagctgctc tcacgggaat accattgtgc cctgcttcat gagcctgatg gtgaggctct
      241 ggcccggaag atttccctca ccttgctgga gaaaggggac ctggacttgg cgtttgtgag
      301 ctgggtttgc aacaggctgc aggtccctga ggtagagagg ggccccaact gcagggacca
      361 tggaggccat actctgtgtg ccaccatgga gctggggtct ccagagggca gctacctgga
      421 gctccttaac agtgatgctg accccctaca cctctaccac ctctatgacc agatggacct
      481 tgctggggag gaagagatcg aactcaactc agagacagac atggacacca tcaactgtga
      541 ccagttcagc aagctgttgc aggatatgga aatggatgaa gagacccggg aggcctatgc
      601 caatatcgcg gaactggatc agtacgtgtt ccaggatacc cagctggagg gcctgagcaa
      661 ggacctcttc atacagcaca ttggaccaga ggaaggcttt ggtgagaacg tagagggccc
      721 tgcagaatca ggagagaagc ctcagaagag acgcttccca gaagagcatg ctatggactc
      781 aaagcacagg aaactagtgc ctccctctag gacctcactg aactatttgg atctccccgc
      841 tgggcatatc cagatcttca ccactctgcc ccagggactc tggcaaatct caggggttgg
      901 cacagggctc tccagtgtcc tagtctacca cggtgagatg ccccaggtca accaattact
      961 ctcttcaagc agccccactg tccccagtct ccccgagtcc ccagaccggc ctggctccac
     1021 cagccccttc gcgccatcca cagctgacct gcccggcatg cctgaacctg ctctgacctc
     1081 ccgtgtaaat gagacagagg acacaacacc cccctcccca tgccaagagg atcccgagcc
     1141 ttccatcaag cttccaaaat ggccagagtc cgtggagcgg ttccagcact ccctacagga
     1201 caagtaccaa gcactgcccg ggagcccaag tggtcctctg gtagccatgg agctggtgcg
     1261 ggccaggttg gaaagaggta gcaacaagag tcaggaaagg gagctggcca ctcctgactg
     1321 gacagagcgc cagctagccc atggcggtct ggccgaggta cttcgggttg tcggcgactc
     1381 caggagatca cgagagacac aggtggtcgc tgtgctgggc aaggctggtc agggcaagag
     1441 ccactgggcc aggacagtga gccacatttg ggcacaaggc cagttaccac aatatgactt
     1501 tgtcttctac gtcccctgtc aatgcttgga tcgccctggg gacacctacc acctgcggga
     1561 tctgctctgt cccccgaacc tgcagccact ggccatggat gacgaggtca ttgatcatat
     1621 tgtgaggcag ccggaccgtg ttctgctcat cctagatgct tttgaggagc tagaggccca
     1681 agacagcctc ctgcatggac cctgtggacc tctgtcccca gagccctgct ccctccgggg
     1741 actgctggct gggctcttcc agcgtaagct gctgcgaggc tgcaccctgc tcctcacagc
     1801 ccggccccgg ggccgcctgg ctcagagcct gagcaaggca gatgccatct tcgaggtgcc
     1861 cagcttctct acgaaacagg cccagatcta tatgaggcac tactttgaga actcagggac
     1921 agcagggagt cacgacaagg ccctgggcct cctggagggc cagccttttc tcctcaccca
     1981 cagtcacagc cctgttctgt gcagtgccgt gtgtcagctc tccaaggccc tgctggatca
     2041 gggcgcagag gcccagctac cttgcacact cacaggactc tatgttggcc tgctaggccc
     2101 tgcagctcag aacagtcctc ctggagcctt agttgagttg gccaagctag cctgggagct
     2161 gggacggagg caccaaagca ccttgcagga aacccagttt ccatccgtgg aagtgaagac
     2221 ctgggctgtg gcccaaggct tgatgcagca gaccacagag gcccaactgg ctttctccag
     2281 ttttctgcta cagtgcttcc tgggtgctgt gtggctggca cagtgcaatg agatgaaaga
     2341 caaggagctg ccacagtacc tggccttgac cccgaggaag aagcgaccct atgacaactg
     2401 gctggagggc gtaccacgct ttctggctgg cttagttttc cagcctcgag cccactgcct
     2461 gggagctctg gtggagcctg caatggctgc agtggcagat aggaaacgga aggttcttac
     2521 caggtacctg aagcgcttga agctggggac acttcgggcc gggaggttgc tggagctgct
     2581 acactgtgcc cacgaaacac aggaacctgg tatttggaag catgtttcac accagctccc
     2641 agggcacctc tccttcctgg gcacacggct cacagcccca gatgtgtatg ttctgggcag
     2701 ggccttggag acagccagcc aggacttctc cttggacctt cgtcagactg gcatcgagcc
     2761 ctccagactg ggagacctcg tgggactcag ctgtgtcacc agtttcaggg cctccttgag
     2821 tgacacaatg gcattatggg agtccctaca gcatcaggga gaaacccagc tactccaggc
     2881 ggcagaggag aagttcacca ttgagccatt taaggccaaa tccccaaagg atgtggaaga
     2941 cctggacagc cttgtgcaga cccagaggct gagaaacccc tcagcggatg cagccaagga
     3001 tcttcctgcc atccgggacc ttaagaagct agagtttgca ttgggccccg tcttgggccc
     3061 gcaggctttc cccacactgg ccaagatcct tccagcattc tcttccctgc agcacctgga
     3121 cctggactca cttagcgaga acaagatcgg ggacaagggt gtgtcgaagc tctcagccac
     3181 cttccctcag ctgaaggccc tggagacgct caacctgtcc cagaatagca tcaccgatgt
     3241 gggagcttgc aagcttgcag aagccctgcc ggccctggcc aagtccctcc taaggctgag
     3301 cttgtacaac aactgcatct gtgacgaagg ggccaagagc ctggcacgag tgcttccaga
     3361 catggtgtcc ctccgggtga tggatgtcca gttcaacaag ttcacggctg tcggggccca
     3421 gcagctgacc tccagccttc agaagtgccc tcaggtggag acactggcaa tgtggacacc
     3481 cactatcccc tttggggtcc aggaacacct gcagcagctg gatgcccgga ttagcctgag
     3541 atgaccctgc tgtaccctgg acaagccgta ctctgggaca ctgaccatgc tggaccttga
     3601 actggatact gtggactcag ctcttctgcg ggccatatcc caggagagtc agcatccgat
     3661 caccctgccc ctgcaggctc agagttgggc cctgcctaca tgaggactgg atacatgtct
     3721 ggttcctctg caggctccaa gaccagctgc aggctcttct ggcaccagct atcttcatgg
     3781 ataccttcat agatgccccg ttctgcatct gtgagcactg gcagacaggc ctggggctcc
     3841 ttcgtgtggg ttcacccagg aggccgaagg ccttctcttc gacattccag gccagtgagg
     3901 aactgaagca tccaagggcc tgccttcctt gtgagaaatc atgtcttccc actgctccca
     3961 gcggcctact ggaggctctg aagatgccaa gggcgatgct gttctgcgac ctctagaggt
     4021 gacagggagc ctatggcagc tctttctact tgtggcactg ttcataaact ttaggtcgaa
     4081 gctgagtcag gcctggccca ggccctgcat tgaaacaaga gccagggcac ggtccacctc
     4141 agggctgcag cgggcagctc ttctggcctc tccttcgcac tcactacact ttaaatgcac
     4201 caagtcccat ctggggacac cttctgtctc atctaatgca aaaacatgca aataaacaaa
     4261 acaagagaaa tcccaaacca gccaaaccac agtaacttaa aacagtccac atctgcccac
     4321 caggcaggct cagccgtctg tctccaccaa ccagaagtca ggctggagtc atcttgagtg
     4381 tggcatgtga cacaggttgc tggctgggag ccccgagtct ttactgggac agctggctgg
     4441 ggctcgtatt ttagctgtgt tataggggga ccacccagag aggagaaagt gaggagacac
     4501 aagccagcag agacagtctg tagactgttc agaacaggcc ttaaggccac acagtatggc
     4561 ttctaccacc ttccaccgca cccttgtgca taccatacag cccaccaccg ctgaggccct
     4621 gtcttcactg caaagatctt cgtagcttcc gaagatgcag tgaaaagctc ttactcccgg
     4681 acctgaggac tgaagcagtg agcaccagac agtggagtgt tctctcctgc tgccttgctg
     4741 catcctggga gcatctctgt agccaaggtg gccagccttg gctgcagcta gcccaaagag
     4801 cttggcagca cctgcgccat tgttcctgtt ttctctggcc accacctttg agctcaggca
     4861 ccattgatcc ctccatctgg acggtgacag cttgctttca tgtcttggca gagggagctg
     4921 tctgtgtgac tccagcatgc cagccctgat gccagcagga ctggcatgtc ccaggaggtg
     4981 ggaactgggc tagagggcct ggggtcgggg gtcaggggtc atggcctctc ggaggtgatt
     5041 cttgaagagg atcctgctgc agctctttac ataagacctg atagggcatc aggctcagcg
     5101 gtggcacatg gaagtcccat gtatgtgacc acaaggggac agcactgagg cactctccaa
     5161 ggcaggcacc tgctgtacct ctgctttgat ctgtctggag atgccaaaat gcctctcatt
     5221 gctgtgttga tgcctgtcat cttaatgagt tgattaggtg aaggcaggga catagtaaat
     5281 gctcaacaaa cctca
//
