LOCUS       NM_052806               2344 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Rattus norvegicus cholinergic receptor nicotinic beta 4 subunit
            (Chrnb4), mRNA.
ACCESSION   NM_052806
VERSION     NM_052806.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Forget,B., Icick,R., Robert,J., Correia,C., Prevost,M.S.,
            Gielen,M., Corringer,P.J., Bellivier,F., Vorspan,F., Besson,M. and
            Maskos,U.
  TITLE     Alterations in nicotinic receptor alpha5 subunit gene
            differentially impact early and later stages of cocaine addiction:
            a translational study in transgenic rats and patients
  JOURNAL   Prog Neurobiol 197, 101898 (2021)
   PUBMED   32841724
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Sun,Y., Li,J., Zheng,C. and Zhou,B.
  TITLE     Study on polymorphisms in CHRNA5/CHRNA3/CHRNB4 gene cluster and the
            associated with the risk of non-small cell lung cancer
  JOURNAL   Oncotarget 9 (2), 2435-2444 (2017)
   PUBMED   29416783
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2344)
  AUTHORS   Han,S., Yang,S.H., Kim,J.Y., Mo,S., Yang,E., Song,K.M., Ham,B.J.,
            Mechawar,N., Turecki,G., Lee,H.W. and Kim,H.
  TITLE     Down-regulation of cholinergic signaling in the habenula induces
            anhedonia-like behavior
  JOURNAL   Sci Rep 7 (1), 900 (2017)
   PUBMED   28420875
  REMARK    Erratum:[Sci Rep. 2017 Dec 1;7(1):17090. doi:
            10.1038/s41598-017-16991-1.. PMID: 29196669]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2344)
  AUTHORS   Chianello Nicolau,M., Pinto,L.F., Nicolau-Neto,P., de Pinho,P.R.,
            Rossini,A., de Almeida Simao,T. and Soares Lima,S.C.
  TITLE     Nicotinic cholinergic receptors in esophagus: Early alteration
            during carcinogenesis and prognostic value
  JOURNAL   World J Gastroenterol 22 (31), 7146-7156 (2016)
   PUBMED   27610024
REFERENCE   5  (bases 1 to 2344)
  AUTHORS   Zhang,X.L., Albers,K.M. and Gold,M.S.
  TITLE     Inflammation-induced increase in nicotinic acetylcholine receptor
            current in cutaneous nociceptive DRG neurons from the adult rat
  JOURNAL   Neuroscience 284, 483-499 (2015)
   PUBMED   25453771
  REMARK    GeneRIF: Results suggest the fast nAChR current in cutaneous dorsal
            root ganglia neurons was carried by homomeric alpha7 nAChRs while
            the slow current was carried by heteromeric (most likely
            alpha3beta4) nAChRs
REFERENCE   6  (bases 1 to 2344)
  AUTHORS   Forsayeth,J.R. and Kobrin,E.
  TITLE     Formation of oligomers containing the beta3 and beta4 subunits of
            the rat nicotinic receptor
  JOURNAL   J Neurosci 17 (5), 1531-1538 (1997)
   PUBMED   9030613
REFERENCE   7  (bases 1 to 2344)
  AUTHORS   Elliott,K.J., Ellis,S.B., Berckhan,K.J., Urrutia,A.,
            Chavez-Noriega,L.E., Johnson,E.C., Velicelebi,G. and Harpold,M.M.
  TITLE     Comparative structure of human neuronal alpha 2-alpha 7 and beta
            2-beta 4 nicotinic acetylcholine receptor subunits and functional
            expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and
            beta 4 subunits
  JOURNAL   J Mol Neurosci 7 (3), 217-228 (1996)
   PUBMED   8906617
REFERENCE   8  (bases 1 to 2344)
  AUTHORS   Boulter,J., O'Shea-Greenfield,A., Duvoisin,R.M., Connolly,J.G.,
            Wada,E., Jensen,A., Gardner,P.D., Ballivet,M., Deneris,E.S.,
            McKinnon,D. et al.
  TITLE     Alpha 3, alpha 5, and beta 4: three members of the rat neuronal
            nicotinic acetylcholine receptor-related gene family form a gene
            cluster
  JOURNAL   J Biol Chem 265 (8), 4472-4482 (1990)
   PUBMED   1689727
REFERENCE   9  (bases 1 to 2344)
  AUTHORS   Duvoisin,R.M., Deneris,E.S., Patrick,J. and Heinemann,S.
  TITLE     The functional diversity of the neuronal nicotinic acetylcholine
            receptors is increased by a novel subunit: beta 4
  JOURNAL   Neuron 3 (4), 487-496 (1989)
   PUBMED   2642007
REFERENCE   10 (bases 1 to 2344)
  AUTHORS   Isenberg,K.E. and Meyer,G.E.
  TITLE     Cloning of a putative neuronal nicotinic acetylcholine receptor
            subunit
  JOURNAL   J Neurochem 52 (3), 988-991 (1989)
   PUBMED   2918319
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Sep 22, 2022 this sequence version replaced NM_052806.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY574260.1, SRR26360176.1368583.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN17050745, SAMN17050746
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               JAXUCZ010000008.1  64332998-64333109   c
            113-261             JAXUCZ010000008.1  64328166-64328314   c
            262-306             JAXUCZ010000008.1  64321127-64321171   c
            307-416             JAXUCZ010000008.1  64320915-64321024   c
            417-1407            JAXUCZ010000008.1  64319196-64320186   c
            1408-2344           JAXUCZ010000008.1  64313669-64314605   c
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..2344
                     /gene="Chrnb4"
                     /note="cholinergic receptor nicotinic beta 4 subunit"
                     /db_xref="GeneID:25103"
                     /db_xref="RGD:2351"
     exon            1..112
                     /gene="Chrnb4"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1548
                     /gene="Chrnb4"
                     /note="neuronal acetylcholine receptor subunit beta-4;
                     N-alpha 2; neuronal acetylcholine receptor non-alpha-2
                     chain; Acetylcholine receptor beta 4; cholinergic
                     receptor, nicotinic, beta polypeptide 4; cholinergic
                     receptor, nicotinic, beta 4 (neuronal); cholinergic
                     receptor, nicotinic beta 4; nicotinic acetylcholine
                     receptor-related protein"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit beta-4
                     precursor"
                     /protein_id="NP_434693.1"
                     /db_xref="GeneID:25103"
                     /db_xref="RGD:2351"
                     /translation="MRGTPLLLVSLFSLLQDGDCRLANAEEKLMDDLLNKTRYNNLIR
                     PATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNIL
                     RIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFP
                     FDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSY
                     VDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFF
                     LLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKE
                     CFLHKLPTFLFMKRPGLEVSLVRVPHPSQLHLATADTAATSALGPTSPSNLYGSSMYF
                     VNPVPAAPKSAVSSHTAGLPRDARLRSSGRFREDLQEALEGVSFIAQHLESDDRDQSV
                     IEDWKFVAMVVDRLFLWVFVFVCILGTMGLFLPPLFQIHAPSKDS"
     exon            113..261
                     /gene="Chrnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            262..306
                     /gene="Chrnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            307..416
                     /gene="Chrnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            417..1407
                     /gene="Chrnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..2344
                     /gene="Chrnb4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgggacata cgctcactcg cggttccagt gtagagtgac cggctgccac ccggctggcc
       61 atgaggggta cgcccctgct cctcgtctct ctgttctctc tgcttcagga cggggactgc
      121 cgcctggcca acgcagagga gaagctgatg gatgacctcc tgaacaaaac ccggtacaac
      181 aacctgatcc gcccagccac cagctcctct cagctcatct ccatccgcct ggagctatca
      241 ctgtcccagc tcatcagtgt gaatgagcga gaacagatca tgaccaccag catctggctg
      301 aaacaggaat ggactgacta ccgcctggcc tggaacagct cctgctatga aggggtgaac
      361 attctgagga tccccgcaaa gcgtgtctgg ttgcctgaca tcgtgttgta caacaatgcc
      421 gatggcacct atgaggtgtc tgtctacacc aacgtgattg tgcgttccaa tggcagcatc
      481 cagtggctgc cccctgctat ctacaagagt gcctgcaaga ttgaggtgaa gcactttccc
      541 ttcgaccagc agaactgcac cctcaaattc cgctcctgga cctatgacca cacggagatt
      601 gacatggttc ttaagtcgcc cacggccatc atggatgact tcacccccag tggtgaatgg
      661 gacattgtgg ccctcccagg acggaggacg gtgaaccctc aggaccccag ctacgtggac
      721 gtgacctatg acttcatcat caagcgcaag ccgctcttct acaccatcaa tcttatcatt
      781 ccttgtgtgc tcatcacctc gctggctatc ctggtcttct acctgccctc cgactgtggg
      841 gagaagatga cgctctgcat ctctgtgctg ctggcactca cgttcttcct gctgctcatc
      901 tccaagatcg tgcctcccac ctcccttgac ataccgctca ttggcaagta cctcttgttc
      961 accatggtgc tggtcacctt ttccatcgtc accactgtgt gtgtcctcaa tgtgcaccac
     1021 cgctcaccca gcactcacac catggcatcc tgggtcaagg agtgcttcct gcacaaactg
     1081 cccaccttcc tcttcatgaa gcgtcccggt cttgaagtca gcctggtcag ggtccctcat
     1141 cccagccagc tgcacttggc cacagctgat actgcagcca cctctgcctt aggccccacc
     1201 agcccatcca acctctatgg gagttccatg tactttgtga accctgtccc tgccgctcct
     1261 aagtctgcag tcagctccca cacagcaggc ctccccaggg atgcccgtct gaggtcctcc
     1321 gggaggttcc gggaagatct acaggaagca ttagagggtg tcagcttcat cgcccagcat
     1381 ctggagagcg atgaccgaga tcaaagtgtc atcgaggact ggaagttcgt cgcgatggtt
     1441 gttgaccgcc tgttcctgtg ggtgttcgtg tttgtgtgta ttctgggcac catggggctc
     1501 ttcctgccac cccttttcca gatccacgca ccctccaagg actcctaggc tacccggcgt
     1561 gtctcgggcc cccgggttgt agtgagatga tatgagaagc cgtgtgggaa gctgactgtt
     1621 ccatttgggc catagctaat gaagccctaa gtaaagccat gactattggc aatcagccca
     1681 tcaaactagc cacagccacg agaaggacag agatagatgc cttctgctga tccctctgat
     1741 gccgtccagt aagtccctga aggaggagga tgaggctgct gaataccagc ctggcctcct
     1801 tccttccacg tgccaggcac cggagtcaga cagccgtgta tcatgccgtc acaccccagc
     1861 ccgccttccc atccatctcc ctctgattcg cagctaccca ctagattccc ccaccatctt
     1921 tcatttccat acacttctag attctcccaa gtcagaacct ttatatcttc tactgctacc
     1981 gtccaaacct cactgtaaac tcccaagctt ttcacgtggc tgcaccagtc agagctctgc
     2041 tccaatgcca cttccttgta taagccttcc catgatcctc tgctccagtg tcatttcctt
     2101 gtataagcct tccctagtca tgcattgcaa agcagtgtct cccataacca catcaaatct
     2161 ccagcttcct tgagtgttcc ccatcagtag ctgcagcctt tagttggtgg cacactagtc
     2221 accagcacct tgcccacaaa agtgtatgct ccataagggc tgggtgtgtg gattcacatt
     2281 cactccagag cttgggaccc agctctgcac acagaaggtg caaaataaat gtttattgaa
     2341 tgaa
//
